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No products found
because this supplier's products are not listed.
Daniyal J Jafree, et al.,
bioRxiv - Pathology 2024
Quote:
... mouse monoclonal anti-platelet and endothelial cell adhesion molecule 1 (PECAM1/CD31 Aligent, GA610, clone: JC70A, 1:50) rabbit polyclonal anti-ssu-2 homolog (SSUH2, Atlas Antibodies, HPA049777, 1:100), rabbit polyclonal anti-fibulin 2 (FBLN2 ...
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No products found
because this supplier's products are not listed.
Ennio d’Amico, et al.,
bioRxiv - Biochemistry 2022
Quote:
The following Dynactin coding genes were codon-optimized for protein expression in insect cells and synthesized (Epoch Life Science): DCTN1 (Protein name p150 ...
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No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Constructs were amplified from insect cell codon-optimized DNA fragments (Bio Basic Inc., Canada) for protein production in High Five insect cells ...
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No products found
because this supplier's products are not listed.
Matthew D. Lauver, et al.,
bioRxiv - Immunology 2022
Quote:
... Virus dilutions were combined 1:1 with 0.45% sheep erythrocytes (Innovative Research) and incubated overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Christine Vazquez, Chin Yee Tan, Stacy M. Horner,
bioRxiv - Microbiology 2019
Quote:
... and rabbit anti-Sendai virus (SV) (1:1000, MBL International). Secondary antibody incubations were done with Alexa Fluor conjugated antibodies (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Anthony C. Fredericks, et al.,
bioRxiv - Microbiology 2019
Quote:
... at the time of cell seeding using TransIT-insect transfection reagent (Cambridge Biosciences, Cambridge, UK) as per manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... goat anti- xenotropic MLV virus antibody ABIN457298 (1:1000, antibodies-online); mouse anti- MLV gag ab100970 (1:1000 ...
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No products found
because this supplier's products are not listed.
Grant M. Zane, et al.,
bioRxiv - Microbiology 2022
Quote:
AAV4 (and AAV5) VLP preparations were validated using the serotype-specific monoclonal antibodies ADK4 (Progen, 610147) and ADK5b (Origene ...
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No products found
because this supplier's products are not listed.
Kranti Konganti, et al.,
bioRxiv - Genomics 2023
Quote:
... and serotype identification was determined following previously published protocols (Fitzgerald et al., 2007; McQuiston et al., 2011).
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No products found
because this supplier's products are not listed.
Joydeb Sinha, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Harvested virus was 0.4μM filtered (Celltreat 229749) and concentrated by centrifugation using a 100kdA cut-off PES protein concentrator (Pierce 88533 ...
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No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... baculovirus gp64 signal sequence under control of the very late polyhedrin promoter was inserted into the insect cell vector plasmid pACEBac1 (Addgene, LGC Standards Teddington, UK) by using another synthetic gene (VK18 ...
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No products found
because this supplier's products are not listed.
Jinsoo Seo, et al.,
bioRxiv - Neuroscience 2020
Quote:
Recombinant human Cdk5 and p25 with N-terminal GST-tags were expressed by Sf9 insect cells via a baculovirus expression system followed by GST affinity purification (BPS Bioscience, San Diego CA).
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No products found
because this supplier's products are not listed.
Bernarda Calla, et al.,
bioRxiv - Genomics 2019
Quote:
... DNA was extracted using an E.Z.N.A.® insect DNA kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Hunter K. Walt, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... insects were individually placed into tubes of RNAzol RT reagent (Molecular Research Center, Cincinnati, OH) and frozen at −80°C for total RNA isolation ...
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No products found
because this supplier's products are not listed.
Bettina P Mihalas, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were incubated in human polyclonal anti-centromere protein antibody (1:50; Antibodies Incorporated, 15-235), rabbit polyclonal anti-SGOL2 antibody (1:200 ...
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No products found
because this supplier's products are not listed.
CE Fain, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was delivered to the right hemisphere of the brain via automatic 1 mL Hamilton syringe (Hamilton Company, Reno, NV). Mice were euthanized for high-dimensional flow cytometry of tissues at 7-days post infection (dpi).
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No products found
because this supplier's products are not listed.
Weigao Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... 300 μL pure protein solution was added to a 1 mm pathlength quartz cuvette (Starna Cells, USA). The scanning wavelength starts from 300 nm and ends at 190 nm ...
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No products found
because this supplier's products are not listed.
Melissa H. Bergeman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Image segmentation and 3D rendering of HSV-1 virus particle undergoing exocytosis from Rab6a vesicle was done in Imaris (Oxford Instruments) by constructing spots and surfaces for the objects at respective image slices ...
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No products found
because this supplier's products are not listed.
Zihao Wang, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
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No products found
because this supplier's products are not listed.
Paul Renauer, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Cellular protein was extracted by treating 1e7 cells / mL in ice-cold 1 x RIPA buffer (Boston BioProducts), containing protease inhibitors (Roche ...
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No products found
because this supplier's products are not listed.
Paul K. LaFosse, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected unilaterally with a stereotactic syringe pump (Stoelting; Wood Dale, IL) through a pulled glass pipette tip cut to an opening of 10–15 μm diameter ...
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No products found
because this supplier's products are not listed.
Chiara Galante, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Red Fluorescent Protein (RFP, rabbit, 1:500, Biomol, 600401379S); SRY-Box 10 (Sox10 ...
-
No products found
because this supplier's products are not listed.
Samuel Herberg, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Defects received 1) three hMSC condensations for a total of 6.0×106 cells with bone morphogenetic protein-2 (BMP-2)-loaded mineral-coated hydroxyapatite (MCM ...
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No products found
because this supplier's products are not listed.
Yuma Nishikawa, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... Protein was extracted from the cells using E.coli/Yeast Protein Extraction Buffer (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), and the lysate fraction was used for analysis.
-
No products found
because this supplier's products are not listed.
Ksenia S. Anufrieva, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... CT26 cells stably expressing near-infrared fluorescent protein eqFP650 (FP731, Evrogen) were injected subcutaneously into female Balb/c mice (12-14 weeks old ...
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WB, IHC,ELISA
Cat# A5233, SKU# A5233-100ul,
100ul, $157.00
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Qiong Wang, et al.,
bioRxiv - Microbiology 2022
Quote:
... Virus quantification by real-time PCR was performed using the QPCR Mix (Bimake, Cat#B21202), following the supplier’s instruction ...
-
No products found
because this supplier's products are not listed.
Chenyan Ma, et al.,
bioRxiv - Neuroscience 2023
Quote:
... starting at day 3 post virus injection (250 mg tamoxifen per kg of chow, Research Diets). Mice were gavaged with two additional doses of tamoxifen (250 mg/kg body weight ...
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No products found
because this supplier's products are not listed.
Callum J. Fraser, et al.,
bioRxiv - Microbiology 2022
Quote:
1 µg of GST tagged protein was added to 1 µg of biotin labelled peptide (EpiCypher) in 300 µL pulldown binding buffer (50 mM Tris [pH 7.5] ...
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No products found
because this supplier's products are not listed.
Jeroen M. Bugter, et al.,
bioRxiv - Cell Biology 2024
Quote:
... followed by 1 h incubation with 25 µL Protein A-agarose beads (RepliGen). Beads were washed five times with 0,1 x PBS after which beads were eluted in SDS sample buffer and incubated for 30 min at 37°C.
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No products found
because this supplier's products are not listed.
Yoshiaki Nishimura, et al.,
bioRxiv - Microbiology 2020
Quote:
... Cells were grown to ~90% confluency in a 96-well plate prior to addition of serially diluted virus to achieve luminescence signals of 30,000-1,000,000 (BMG Labtech PolarStar Optima plate reader at maximum gain) ...
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No products found
because this supplier's products are not listed.
Lakshmi E. Batachari, et al.,
bioRxiv - Immunology 2024
Quote:
... samples were incubated with Quasar 670-conjugated FISH probes that hybridize to Orsay virus RNA1 and RNA2 (Biosearch Technologies). Following a 6-8 h incubation at 47 °C ...
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No products found
because this supplier's products are not listed.
Jennine M. Dawicki-McKenna, et al.,
bioRxiv - Molecular Biology 2022
Quote:
Total protein extraction from cells was performed using 1.5x Laemmli buffer [15% glycerol (Amresco #M152), 3% SDS (Sigma #L5750) ...
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No products found
because this supplier's products are not listed.
Kevin J. Kramer, et al.,
bioRxiv - Immunology 2021
Quote:
... values at the endpoint (48 h after incubation with the virus) were determined using the RTCA software version 2.1.0 (ACEA Biosciences Inc.). Results are expressed as percent neutralization in a presence of respective antibody relative to control wells with no CPE minus CI values from control wells with maximum CPE ...
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CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor...
Cat# CSC 2F0 V,
1.0 Units, $405.0
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Ruth Haverty, et al.,
bioRxiv - Microbiology 2023
Quote:
... supplemented with 1% CultureBoost™ (Cell Systems), and 0.05% Bac-Off® (Cell Systems) ...
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No products found
because this supplier's products are not listed.
Shafali Gupta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Rabbit antibodies against the following proteins were used: GAPDH (Trevigen 2275-PC-100; 1:5000), p114 RhoGEF (Abcam ab96520 ...
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No products found
because this supplier's products are not listed.
Derek C. Rookhuizen, et al.,
bioRxiv - Immunology 2021
Quote:
... Cells were cross-linked with 1% formaldehyde (Euromedex) for 8 minutes at room temperature and the reaction quenched in 150mM glycine for 10 minutes ...
-
No products found
because this supplier's products are not listed.
Hafsa Munir, et al.,
bioRxiv - Cancer Biology 2020
Quote:
1×105 neutrophils were treated with CAF CMed diluted 1:1 in complete EC media (Cell Biologics) with or without Cl-amidine and incubated for 3h to generate NETs ...
-
No products found
because this supplier's products are not listed.
Qimeng Li, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Proteins were produced as periplasmic components in 1 L of the NZY Auto-Induction TB medium (NZytech) according to the manufacturer’s recommendations and were purified by immobilized metal affinity chromatography on a HiTrap TALON® crude 1 mL column (Cytiva) ...
-
No products found
because this supplier's products are not listed.
Brunno R. Levone, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Proteins were quantified using a validated BCA (Bicinchoninic Protein Assay Kit, EuroClone) protocol ...
-
No products found
because this supplier's products are not listed.
Tao Zou, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... cells were treated with increasing doses of recombinant human IFN-beta 1a (mammalian) protein (PBL Assay Science, #114151) or sterile water as the vehicle control ...
-
No products found
because this supplier's products are not listed.
Sarah K. Schultz, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Cell opening and fluorescent labeling of L-AHA-containing proteins with DBCO-PEG4-Carboxyrhodamine 110 (Click Chemistry Tools) were performed as described 29 ...
-
No products found
because this supplier's products are not listed.
Flavia Ferrantelli, et al.,
bioRxiv - Microbiology 2022
Quote:
... virus dilutions and number of deaths/group after challenge were used to calculate the LD50 (Quest Graph™ LD50 Calculator, AAT Bioquest, Inc.). The Kaplan-Meier curve was used to show survival rate differences among groups of animals challenged with different doses of SARS-CoV-2 virus ...
-
No products found
because this supplier's products are not listed.
Yafei Liu, et al.,
bioRxiv - Microbiology 2020
Quote:
A pME18S expression plasmid containing the full-length or subdomain spike protein was transiently transfected into HEK293T cells using PEI max (PolyScience); the pMx-GFP expression plasmids were used as the marker of transfected cells ...
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With the RapidClean protein removal kit, completely remove protein from aqueous solutions of...
Cat# K-01001-010,
10 reactions, USD $145.00/ea
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L. Perrin, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... antibodies diluted in 5% non-fat milk/TBST for 1 h at room temperature and proteins were visualized using chemiluminescence detection reagents (WesternBright, Advansta) and blot scanner (C-DiGit ...
-
No products found
because this supplier's products are not listed.
Logan R. Hurst, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Twenty microliters of this cell suspension was then mixed 1:1 with 0.6% low-melt agarose (RPI) warmed to 50°C ...
-
No products found
because this supplier's products are not listed.
Stefano Musardo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cells were incubated with primary antibody (Oxytocin, 1/10000 dilution, Immunostar #20068 ...
-
No products found
because this supplier's products are not listed.
Paul R. Marshall, et al.,
bioRxiv - Neuroscience 2019
Quote:
... Lysate from cells or tissue was fixed in 1% formaldehyde and cross-linked cell lysates were sheared by Covaris in 1% SDS lysis buffer to generate chromatin fragments with an average length of 300bp ...
-
No products found
because this supplier's products are not listed.
Ryan M. Carr, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... incubated in protein block (Rodent Block M, Biocare) for 30 minutes ...
-
No products found
because this supplier's products are not listed.
Stefan Dannenmaier, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Polyclonal antibodies were raised against the fusion protein (Eurogentec). Immuno-reactive complexes were detected using horseradish-peroxidase coupled anti-rabbit or anti-mouse antibodies (Sigma-Aldrich/Merck ...
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No products found
because this supplier's products are not listed.
Kimberley R.G. Cortenbach, et al.,
bioRxiv - Immunology 2021
Quote:
... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...