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Citations for Click Chemistry Tools :
1 - 50 of 76 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... Cell opening and fluorescent labeling of L-AHA-containing proteins with DBCO-PEG4-Carboxyrhodamine 110 (Click Chemistry Tools) were performed as described 29 ...
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bioRxiv - Biochemistry 2020Quote: ... Enrichment of alkyne-modified proteins was conducted using the Click-&-Go Protein Enrichment Kit (Click Chemistry Tools) and the protocols thereof ...
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bioRxiv - Microbiology 2020Quote: ... to AHA containing proteins was carried out using Click-&-Go™ Protein Reaction Buffer Kit (Click Chemistry Tools) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... L-AHA-containing proteins were labeled with Cy7 by strain-promoted azide-alkyne click-chemistry employing 1 μM dibenzylcyclooctyne-Cy7 (DBCO-Cy7) (Click Chemistry Tools, USA, Scottsdale). After methanol-chloroform extraction ...
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bioRxiv - Physiology 2023Quote: ... Enriched proteins were eluted for mass spectrometry analysis according to the Click-&-GoTM Dde Protein Enrichment Kit (Click Chemistry Tools, 1153).
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bioRxiv - Biochemistry 2022Quote: ... Fluorophores for protein labeling were from Click Chemistry Tools Inc.
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bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
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bioRxiv - Microbiology 2023Quote: ... using the Click-&-Go protein enrichment kit (Click Chemistry Tools) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Alkyne-conjugated biotin and Protein Reaction Buffer kit were from Click Chemistry Tools.
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bioRxiv - Biochemistry 2021Quote: Protein pellets functionalized with the TAMRA-Azide-Desthiobiotin (Click Chemistry Tools, Scottsdale, AZ) were precipitated by methanol/chloroform as described above and resuspended in 500 μL of 6M Urea + 25 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... was conducted using the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools 1262) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
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bioRxiv - Cell Biology 2020Quote: ... The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools) and AF488 alkyne (Click Chemistry Tools ...
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bioRxiv - Immunology 2023Quote: ... beads (now containing bound protein) were washed and incubated with 5 μM DBCO-488 (Click Chemistry Tools) for 30 min at RT or overnight at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Molecular Biology 2021Quote: ... brucei cells (0.7-0.9 × 106 cells/ml) were incubated with 150 µM 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools) for 3 hrs before genomic DNA was isolated ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were labeled with 20μM EdU (Click Chemistry Tools) for 20 min and EdU was clicked to a mixture of Alexa488 and biotin azides at molar ratio of 1 to 50 ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
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bioRxiv - Bioengineering 2022Quote: ... and Click-&-Go Cell Reaction Buffer Kit (Click Chemistry Tools), and 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Cell Biology 2020Quote: Immortalized myeloid cells were treated with 10µM OPP (Click Chemistry tools) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 24 μM carboxyrhodamine 110 Alkyne (Click Chemistry Tools, TA106-1). Cells were washed 3x in PBS before acquisition via FACS or ImageStream.
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bioRxiv - Physiology 2023Quote: ... Cells were then lysed and clicked to either rhodamine azide (Click Chemistry Tools) for in-gel fluorescence ...
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bioRxiv - Neuroscience 2020Quote: ... with 25 μM Alkyne Alexa fluor 405 (Click Chemistry tools # 1309-1), CuSO4 and additive overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and 100× molar equivalents of DBCO-AF647 (Click Chemistry Tools, 1302-1) in DMSO were added and the reaction was mixed at 500 rpm in the dark for 2 hours rt ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with 5 μM picolyl azide dye (Click Chemistry Tools, Scottsdale, AZ) for 10 minutes at room temperature.
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bioRxiv - Physiology 2023Quote: ... Cells selectively labeled with 5 μM picolyl azide dye (Click Chemistry Tools, Scottsdale, AZ) were incubated for 10 minutes at room temperature ...
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... cell lysates prepared as above were labeled with azido-PEG3-biotin (Click Chemistry Tools, AZ104), incubated with high-capacity neutravidin beads (Thermo Scientific Pierce) ...
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bioRxiv - Microbiology 2022Quote: ... 5-EU was labeled in permeabilized cells using the Click & Go Kit (Click Chemistry Tools #1263) with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275 ...
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bioRxiv - Bioengineering 2020Quote: ... incubated for 40 minutes in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... both transfected and untransfected control cells were treated with 50 µM O-Propargyl Puromycin (OPP, Click Chemistry Tools) for 10 min at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed in PBS then subjected to CuAAC with picolyl azide-AF488 or picolyl azide-TAMRA (Click Chemistry Tools) as described (67 ...
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bioRxiv - Cell Biology 2023Quote: ... cells are incubated overnight in culture medium with 5 μM of 5-ethynyl 2’-deoxyuridine (EdU, #1149, Click Chemistry Tools) diluted from a 10 mM stock in DMSO ...