-
No products found
because this supplier's products are not listed.
Fabrizio Clarelli, et al.,
bioRxiv - Biochemistry 2019
Quote:
... and CRP (Mouse α-E. coli CRP, 664304, Nordic Biosite antibodies) were diluted 1:250 ...
-
No products found
because this supplier's products are not listed.
MD Fahlberg, et al.,
bioRxiv - Immunology 2020
Quote:
... and Kynurenine ELISA commercial kits (Rocky Mountain Diagnostics, Colorado Springs ...
-
No products found
because this supplier's products are not listed.
Courtney L. Finch, et al.,
bioRxiv - Microbiology 2020
Quote:
... and blocked with ELISA diluent (5% nonfat milk [LabScientific, Danvers, MA, USA] in PBS-T) for 1 h at 37°C ...
-
No products found
because this supplier's products are not listed.
Estrela Neto, et al.,
bioRxiv - Cell Biology 2020
Quote:
... a multi-neurotrophin rapid screening ELISA kit (#BEK-2231, Tebu-bio, France) was used according to the manufacturers’ protocol ...
-
No products found
because this supplier's products are not listed.
Noelia Perez Diaz, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... bronchi and parenchyma from naïve rats was measured using Rat PPARβ/δ ELISA kit (Abbkine) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Danya Abazari, et al.,
bioRxiv - Neuroscience 2022
Quote:
Protein palmitoylation assay was performed using CAPTUREome S-palmitoylated protein kit (Badrilla, Leeds, UK), as described by manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Zachary JW Easton, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... PVDF membranes were blocked in 5% dry-milk protein or 5% Bovine Serum Albumin (BSA, BioShop Canada Inc., Burlington, Canada) and membranes were incubated overnight at 4℃ with respective antibody solutions (Supplementary Table 2) ...
-
No products found
because this supplier's products are not listed.
Christian M. Smolko, Kevin A. Janes,
bioRxiv - Molecular Biology 2019
Quote:
... and the plate incubated for 1 hr at 37°C on a Jitterbug mixer (Boekel Scientific #130000) with mix setting set to 1 ...
-
No products found
because this supplier's products are not listed.
Ajay Bhat, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... we added 2.5 mM or 5 mM paraquat to FUdR plates not containing antibiotic (40690016, Bioworld). Equal O.D of bacteria were seeded and allowed to dry for 48 hours prior to transferring worms to the plates ...
-
No products found
because this supplier's products are not listed.
Jing Li, et al.,
bioRxiv - Cell Biology 2019
Quote:
... the culture was maintained at 37°C/5% CO2 in Complete Fibroblast Medium (Cat. M2267, Cell Biologics Inc.) containing growth factor supplement and 10% fetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
Julia Fath, et al.,
bioRxiv - Cell Biology 2022
Quote:
Cells were cultured in a 12 well-plate and incubated with 1 μM TAMRA-C-ter BDNF (TAMRA-KKRIGWRFIRIDTSCVCTLTIKRGR-COOH; Proteogenix, France) for 40 minutes in incubation chamber at 37°C in a 5% CO2 atmosphere ...
-
No products found
because this supplier's products are not listed.
Ambre Guillory, et al.,
bioRxiv - Plant Biology 2024
Quote:
... root samples were incubated overnight in the dark at 28 °C in Z-buffer containing 2 mM Magenta-Gal (5-bromo-6-chloro-3-indoxyl-b-D-galactopyranoside; B7200; Biosynth) or X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside ...
-
No products found
because this supplier's products are not listed.
Mia P. Sams, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Samples were denatured at 100°C and separated on 10% SDS-PAGE gels with BLUeye Prestained Protein Ladder (FroggaBio, Concord ON). Protein was then semi-dry transferred to a PVDF membrane and incubated in Li-Cor Intercept® Blocking Buffer (Cedarlane ...
-
No products found
because this supplier's products are not listed.
Sherif Salah, et al.,
bioRxiv - Immunology 2022
Quote:
Different SARS-CoV-2 coronavirus antigen concentrations (5, 10, 20, 40, and 80 μg/ml) of spike protein (S1) (by ProSci Inc.), nucleocapsid protein ...
-
No products found
because this supplier's products are not listed.
Mohammed Samer Shaban, et al.,
bioRxiv - Immunology 2020
Quote:
... HuH7 and MRC-5 cells were confirmed to be free of mycoplasma using the Venor® GeM Classic kit (Minerva Biolabs).
-
No products found
because this supplier's products are not listed.
Rhett A. Snyder, et al.,
bioRxiv - Microbiology 2019
Quote:
... Quantification of c-di-GMP in sample extracts was determined using a standard curve generated from chemically synthesized c-di-GMP (AXXORA). The standard curve solutions were prepared using twofold serial dilutions of c-di-GMP (1.25 μM – 19 nM ...
-
No products found
because this supplier's products are not listed.
Zoila A. Lopez-Bujanda, et al.,
bioRxiv - Immunology 2019
Quote:
... Knock out clones were screened for IL-8 and Cxcl15 expression by ELISA and gene-editing confirmed by PCR amplification and Sanger sequencing (GENEWIZ) using primers ∼200bp away from the cut site (IL-8 Forward ...
-
No products found
because this supplier's products are not listed.
Sven Klumpe, et al.,
bioRxiv - Biophysics 2021
Quote:
... 5 ug/ml Insulin (Cell Applications), 10 mM HEPES (pH 7.4) ...
-
No products found
because this supplier's products are not listed.
Jessica Rodgers, et al.,
bioRxiv - Neuroscience 2020
Quote:
... As this plate reader has a single photomultiplier tube (PMT), light emitted by fluorescent Venus and bioluminescent Nanoluc were measured sequentially using 535nm (30nm FWHM with gain set to 4095 ...
-
No products found
because this supplier's products are not listed.
Carina C D Joe, et al.,
bioRxiv - Bioengineering 2021
Quote:
Residual host-cell protein (HCP) was quantified using the HEK293 HCP ELISA kit (Cygnus Technologies) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Stefan Haug, et al.,
bioRxiv - Genetics 2022
Quote:
... polyclonal rabbit primary antibodies reactive against POU3F3 (Atlas Antibodies, HPA067151) were applied to the tissue sections at 0.5 µg/ml overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... Cell supernatants were screened by ELISA using recombinant YFV E protein (Meridian Life Sciences). Wells with positive reactivity were fused to a human-mouse myeloma cell line (HMMA 2.5 ...
-
No products found
because this supplier's products are not listed.
Razieh Rafieenia, et al.,
bioRxiv - Microbiology 2022
Quote:
... Glyphosate concentrations were measured using a glyphosate ELISA kit (Abraxis, Eurofin Technologies, Hungary).
-
No products found
because this supplier's products are not listed.
Laura N. Puentes, et al.,
bioRxiv - Neuroscience 2020
Quote:
... BioPORTER Protein Delivery Reagent “QuikEase Kit” (Genlantis, Cat#BP502424) was prepared according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Elena A. Andreeva, et al.,
bioRxiv - Biophysics 2022
Quote:
... the protein solution (∼ 5 ml) was circulated with a peristaltic pump (Gilson Minipuls 3) through a 2 mm X-ray quartz capillary in a closed loop ...
-
No products found
because this supplier's products are not listed.
Jonathan H Massey, et al.,
bioRxiv - Genetics 2019
Quote:
... 5 flies were placed in a single glass vial (Wheaton 224740 E−C Clear Glass Sample Vials) on ice ...
-
No products found
because this supplier's products are not listed.
Julian G. Dishart, et al.,
bioRxiv - Neuroscience 2023
Quote:
... L4 populations were washed off plates in M9 and seeded onto pre-treated (+)-5-Fluorodeoxyuridine (FUDR; Spectrum Chemical) OP50 plates ...
-
No products found
because this supplier's products are not listed.
El Batoul Djouani-Tahri, et al.,
bioRxiv - Plant Biology 2023
Quote:
... Total cellular proteins were labeled by adding 5 μCi mL-1 of [35S] Na2SO4 (American Radiolabeled Chemicals) for 10 min ...
-
No products found
because this supplier's products are not listed.
Jessy Carol Ntunzwenimana, et al.,
bioRxiv - Genetics 2021
Quote:
... Stable shRNA DUSP16 knockdown cell lines were derived in triplicate for each shRNA vector following selection with 3 ug/ml puromycin and were maintained at 37°C with 5% atmospheric CO2 in McCoy’s 5A (Modified) medium (cat no 317-010-CL, Wisent Bioproducts) supplemented with 10% FBS (cat F1051-500mL ...
-
No products found
because this supplier's products are not listed.
Megan Garland, et al.,
bioRxiv - Microbiology 2019
Quote:
... Frozen stocks were cultured on CDMN agar plates (C. difficile agar base (Oxoid CM0601) supplemented with 7% (v/v) defibrinated horse blood (Lampire Biological Laboratories), 32 mg/L moxalactam (Santa Cruz Biotechnology) ...
-
No products found
because this supplier's products are not listed.
Ghada Elfayres, et al.,
bioRxiv - Microbiology 2023
Quote:
HEK293T cells were seeded into 10-cm plates 24 h prior to transfection with pEZY3 plasmids expressing SARS-CoV-2 proteins using polyethyleneimine (PEI; PolyScience). For this ...
-
No products found
because this supplier's products are not listed.
Mark S. Lee, et al.,
bioRxiv - Immunology 2023
Quote:
... 5×104 transduced 58α-β- T cell hybridomas were cocultured with 1×105 transduced I-Ek+ M12 cells in triplicate in a 96 well round bottom plate in RPMI with 5% FBS (Omega Scientific), Pen-Strep+L- Glutamine (Cytiva) ...
-
No products found
because this supplier's products are not listed.
M. Kyle Cromer, et al.,
bioRxiv - Genetics 2021
Quote:
... The sgRNA modifications added were the 2’-O-methyl-3’-phosphorothioate at the three terminal nucleotides of the 5’ and 3’ ends described previously38. All Cas9 protein (SpyFi S.p. Cas9 nuclease) was purchased from Aldevron, LLC (Fargo ...
-
No products found
because this supplier's products are not listed.
Yusuke Ohno, et al.,
bioRxiv - Cell Biology 2023
Quote:
... was incubated at 25 °C for 5 min and subjected to 1H NMR spectrometry at 400 MHz (JNM-ECZ400; JEOL, Tokyo, Japan).
-
No products found
because this supplier's products are not listed.
Florian J. Bock, et al.,
bioRxiv - Cell Biology 2020
Quote:
... S63845 (Chemgood, C-1370), Sytox Green (Thermo ...
-
No products found
because this supplier's products are not listed.
Yang-Xue Dai, et al.,
bioRxiv - Biophysics 2021
Quote:
... Crystallization screening was carried out at 20 °C using commercial screening kits (Hampton Research, Molecular Dimensions and Rigaku Reagents), where the ToPif1-DNA complex was mixed at a 1:1 ratio with the reservoir solution ...
-
No products found
because this supplier's products are not listed.
Aleksi Isomursu, et al.,
bioRxiv - Biophysics 2020
Quote:
Cells that were collected for protein lysates were cultured on commercial hydrogel-coated 6-well plates (Matrigen, SW6-EC-0.5/SW6-EC-8/SW6-EC-50). These gels were similarly coated with 10 μg/ml of fibronectin before use.
-
No products found
because this supplier's products are not listed.
Tongcui Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
... or conjugated in-house with X8 antibody-labeling kits (Standard BioTools) and stored at 4°C in Antibody Stabilizer (Boca Scientific) supplemented with 0.05% sodium azide ...
-
No products found
because this supplier's products are not listed.
Shuang Fu, et al.,
bioRxiv - Biophysics 2022
Quote:
... Ara-C (2 mM, TargetMol, T1272) was added to the medium to inhibit neural stem cell proliferation ...
-
No products found
because this supplier's products are not listed.
Armin Bayati, et al.,
bioRxiv - Cell Biology 2022
Quote:
... was then conjugated with 5 nm gold beads (Cytodiagnostics, cat# CGN5K-5-2), immediately before experimental use ...
-
No products found
because this supplier's products are not listed.
Bo Fan, et al.,
bioRxiv - Bioengineering 2020
Quote:
... post-baked (65 °C and 95 °C for 1 min and 4 min, respectively) and developed in SU-8 developer (MicroChem). The SU-8 was hard-baked at 180 °C for 1 hour after development ...
-
No products found
because this supplier's products are not listed.
Donggi Paik, et al.,
bioRxiv - Immunology 2021
Quote:
... for 96-well plate cultures or a turbovap (Biotage) for bacterial tube cultures or eppendorf tube lysates ...
-
No products found
because this supplier's products are not listed.
Ved Mehta, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Precipitated sodium deoxycholate was removed by filtering through a Corning® 2 µM PVDF plate and samples were further desalted on a 96-well MacroSpin plate (The Nest Group). Peptides were eluted with 80% acetonitrile ...
-
No products found
because this supplier's products are not listed.
Ian C. Miller, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 5% human AB serum (Valley Biomedical #HP1022), 10 mM N-acetyl L-Cysteine (Sigma #A9165) ...
-
No products found
because this supplier's products are not listed.
Kristine L Trotta, et al.,
bioRxiv - Microbiology 2023
Quote:
... Protein was transferred to nitrocellulose (0.2µm; GVS) via semi-dry transfer with a TransBlot Turbo transfer system (BioRad ...
-
No products found
because this supplier's products are not listed.
Maria Alexandra Rujano, et al.,
bioRxiv - Developmental Biology 2021
Quote:
Measures of fluorescence intensities over time (Fig. 5 and Supp. Fig. 5-1c) were performed on Volocity 6.3 (Quorum technologies). For each region (GFP only ...
-
No products found
because this supplier's products are not listed.
Hyun Jun Woo, et al.,
bioRxiv - Microbiology 2019
Quote:
... was grown on Brucella agar plates (Becton-Dickinson, Braintree, MA, USA) supplemented with 10% bovine serum (BRL Life Technologies ...
-
No products found
because this supplier's products are not listed.
Alejandra Mondino, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Injections were performed at a rate of 5 nL/min using a Hamilton Neuros Syringe 7000 (5 mL; Hamilton Company, Reno, NV, USA) mounted on a microinjection syringe pump ...
-
No products found
because this supplier's products are not listed.
M. Martinez, et al.,
bioRxiv - Microbiology 2023
Quote:
... at 4°C and loaded 3 times in a CellD disrupter (Constant Systems). The lysate was centrifuged for 15min at 12000 x g at 4°C to remove cell debris and the supernatant was centrifuged again for 1h at 100000 x g at 4°C ...
-
No products found
because this supplier's products are not listed.
Garth Blackler, et al.,
bioRxiv - Physiology 2023
Quote:
... overnight at 4°C and decalcified in Decal Stat (StatLab, PC-1212-1) for 24 hours ...