-
No products found
because this supplier's products are not listed.
Alyssa R. Phillips, et al.,
bioRxiv - Plant Biology 2023
Quote:
... and 1.25% pectolyase Y-23 (Desert Biologicals) in citrate buffer (10 mM sodium citrate ...
-
No products found
because this supplier's products are not listed.
Charlène Iltis, et al.,
bioRxiv - Immunology 2021
Quote:
... membranes were stripped at 4°C in agitation using Antibody stripping buffer 1X for 10 minutes (Gene Bio-Application). Protein bands were quantified using ImageJ software ...
-
No products found
because this supplier's products are not listed.
Narayan Pokhrel, et al.,
bioRxiv - Evolutionary Biology 2022
Quote:
... were collected from a commercial farm and stored at 18°C or 12°C up to 28 d in a cooled incubator (SN 265959, VELP SCIENTIFICA, Italy). The temperature and relative humidity of cooled incubator was continuously monitored throughout the experiment ...
-
No products found
because this supplier's products are not listed.
Bettina M. Fuglerud, et al.,
bioRxiv - Genomics 2021
Quote:
... at 4 °C overnight in CHAPS immunoprecipitation buffer (Fivephoton Biochemicals). After washing in TBST ...
-
No products found
because this supplier's products are not listed.
Jason Yang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... supplemented with 100 μg/mL carbenicillin (AG Scientific C-1385) in 96-well polypropylene plates overnight at 37 °C ...
-
No products found
because this supplier's products are not listed.
Kévin Leguay, et al.,
bioRxiv - Cell Biology 2020
Quote:
Coelenterazine 400a (Deep Blue C) and methoxy e-CTZ (Prolume Purple) were purchased from NanoLight Technology (#340 and #369 ...
-
No products found
because this supplier's products are not listed.
Christian S. Stevens, et al.,
bioRxiv - Microbiology 2022
Quote:
... and fluorescent probe (5’-6FAM-ACAGACGTTGTATA+C+CAT+G-TMR) (TIB MOLBIOL) RT-qPCR was performed using the following cycle ...
-
No products found
because this supplier's products are not listed.
Helen J. von Richthofen, et al.,
bioRxiv - Immunology 2022
Quote:
... Cells were treated 2h at 37°C with neutrophil elastase (Elastin Products Company), cathepsin G (Biocentrum) ...
-
No products found
because this supplier's products are not listed.
Erika Riederer, et al.,
bioRxiv - Biophysics 2021
Quote:
... 1 µM valinomycin at 30 °C using 100 nM of 14C Asp (Moravek Biochemicals) as previously described.(33 ...
-
No products found
because this supplier's products are not listed.
John Marshall, et al.,
bioRxiv - Neuroscience 2023
Quote:
... A microsyringe with a 23 gauge flat tip needle (Hamilton Company, Reno, NV) with a manual stereotaxic injector (Stoelting ...
-
No products found
because this supplier's products are not listed.
Armand O. Brown, et al.,
bioRxiv - Microbiology 2020
Quote:
... inflammatory chemokines and cytokines were additionally analyzed using a Mouse Cytokine ELISA Plate Array III Colorimetric Assay (Signosis). The data represent an average of at least 3 independent experiments for each strain and were analyzed using Student’s two-tailed t-test.
-
No products found
because this supplier's products are not listed.
Eva Gracia Villacampa, et al.,
bioRxiv - Genetics 2020
Quote:
... the slides were dried at 40°C in a hybridization oven (HB-1000 Hybridizer, LabRepCo) for 1 hour and 45 min ...
-
No products found
because this supplier's products are not listed.
Luis F. Queme, et al.,
bioRxiv - Neuroscience 2019
Quote:
... 20 mg/kg of xx-650-23 (GLXC-12046, Glixx lab, Hopkinton, MA, USA) or an equivalent volume of vehicle (0.1% DMSO ...
-
No products found
because this supplier's products are not listed.
Y Audrey Hay,
bioRxiv - Neuroscience 2021
Quote:
... France) mice temperature was monitored and maintained at 34-36 °C using a heating blanket (Basi). A small burr hole was drilled at LDTg coordinates AP = -4.92 mm and ML = 1 mm and an optical fiber (300 μm ...
-
No products found
because this supplier's products are not listed.
Adrian S. Monthony, et al.,
bioRxiv - Plant Biology 2020
Quote:
... The callus induction medium (hereafter referred to as LT-C) consisted of MS (M524; Phytotechnology Laboratories) nutrients ...
-
No products found
because this supplier's products are not listed.
Kelsey M. Tyssowski, Jesse M. Gray,
bioRxiv - Neuroscience 2019
Quote:
... The temperature was maintained by putting the plate on a 37°C warming plate (Bel-Art). The CO2 was maintained at 5% throughout the duration of the recording using the base provided with the Axion Lumos system ...
-
No products found
because this supplier's products are not listed.
Tamás Bakos, et al.,
bioRxiv - Immunology 2024
Quote:
... Soluble terminal C complex (sTCC, sC5b9) was measured with an ELISA kit from Svar Life Science AB (Malmö ...
-
No products found
because this supplier's products are not listed.
Rubika Balendra, et al.,
bioRxiv - Neuroscience 2022
Quote:
Poly(PR) 20-mer dipeptide repeat protein with a C-terminal FLAG tag was purchased from CSBio and verified by mass spectrometry ...
-
No products found
because this supplier's products are not listed.
Tomoki Togashi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... hPC antigen (hPC:Ag) was measured using the Human Protein C AssayMaxTM ELISA Kit (Assaypro, St. Charles, MO) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Tobias Butelmann, et al.,
bioRxiv - Bioengineering 2021
Quote:
... an LM was taken out from the medium (30 °C) and weighed on a precision scale (Adam Equipment, UK). To determine the dry weight of the cells ...
-
No products found
because this supplier's products are not listed.
Justin L. Tosh, et al.,
bioRxiv - Neuroscience 2020
Quote:
... ranging from 3678 to 3.6 pg/ml of a synthetic dimer with the Aβ1-11 sequence (DAEFRHDSGYE) C-terminally linked via a cysteine residue (28) was ordered from rPeptide and supplied by Stratech.
-
No products found
because this supplier's products are not listed.
Alex Quintero-Yanes, Aurélie Mayard, Régis Hallez,
bioRxiv - Microbiology 2022
Quote:
... Temperature (30 °C) was maintained stable during microscopy analysis using the Tempcontrol 37-analog 1 channel equipment (HemoGenix®) coupled to the Axio Imager Z1 microscope ...
-
No products found
because this supplier's products are not listed.
Zi Ye, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Defibrinated sheep blood was stored at 4°C and used within 2 weeks of purchasing (Hemostat Laboratories, Dixon, CA). Human foot odorants were provided as cloth strips cut from socks that had been continually worn for 5 days by a 30-year-old male volunteer and thereafter incubated overnight at 37°C in a sealed Ziploc plastic bag (SC Johnson ...
-
No products found
because this supplier's products are not listed.
Zahra Hosseinzadeh, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The insulin of each sample was measured with the C-Peptide & Insulin AccuBind VAST ELISA Kits (Monobind Inc., USA). Three independent experiments were performed for each group ...
-
No products found
because this supplier's products are not listed.
Zachary D. Whiddon, et al.,
bioRxiv - Neuroscience 2023
Quote:
... or 23 days with vehicle (PEG 400:5% dextrose in water) or LDE225 (NVP-LDE225, ChemieTek, Indianapolis, IN) dissolved in vehicle at a dose of 20 mg/kg ...
-
No products found
because this supplier's products are not listed.
Peter A. van der Ley, et al.,
bioRxiv - Immunology 2021
Quote:
The prefusion stabilized HexaPro spike protein27 with and without a 3xGGGS spacer and mCRAMP sequence added to the C-terminus were produced by Celonic AG (Basel ...
-
No products found
because this supplier's products are not listed.
Marissa M. Gaskill, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... attP40{nos-Cas9}/CyO for the N-terminal line and y1 M{vas-Cas9.RFP-}ZH-2A w1118 (BDSC#55821) for the C-terminal line by BestGene Inc ...
-
No products found
because this supplier's products are not listed.
Cornelia Blume, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... Data were normalised to the geometric mean of the housekeeping genes (ubiquitin C and glyceraldehyde 3-phosphate dehydrogenase, probe-based duplex primer mix, PrimerDesign) and fold change in gene expression relative to controls was determined using the ΔΔCt method ...
-
No products found
because this supplier's products are not listed.
Yongchan Lee, et al.,
bioRxiv - Biochemistry 2019
Quote:
... UniProt ID Q01650) and CD98hc isoform c (SLC3A2 isoform 1; UniProt ID P08195-1) were amplified from human universal reference cDNA (ZYAGEN) and cloned individually into the pEG BacMam vector (84) ...
-
No products found
because this supplier's products are not listed.
Jugal Mohapatra, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and heated to 90 °C for 2 min while bubbling with nitrogen gas in N,N-dimethylformamide (DMF, Oakwood Chemical). Fmoc deprotection was carried out with 20% piperidine (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Isabelle Petit-Härtlein, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 0.1 µL of protein solution and 0.1 µL of reservoir were mixed to equilibrate against 45 µL reservoir solution at 20°C in 96-well CrystalDirect plates (MiTeGen) and automatically imaged in RockImager robot (Formulatrix) ...
-
No products found
because this supplier's products are not listed.
Harsimranjit Sekhon, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... All sensor and ligand proteins except for SH2 were purified by nickel-nitrilotriacetate chromatography according to the manufacturer’s instructions (Molecular Cloning Laboratories). SH2 did not have a His-Tag and was purified using an SP Sepharose column as described37 ...
-
No products found
because this supplier's products are not listed.
Samuel Schmidt, et al.,
bioRxiv - Cell Biology 2020
Quote:
... incubated 24h at 4 °C) were used as substrates and prepared in Willco dishes (35 mm, Willco Wells B.V., HBSt-3522) as described46 ...
-
No products found
because this supplier's products are not listed.
Tomasz Zieliński, et al.,
bioRxiv - Biophysics 2022
Quote:
... and kept in the CO2 (37°C and 5%CO2/95% air atmosphere) for 24 hours in the DMEM (ATCC, LGC Standards) supplemented with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Anna Vilalta, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Proteins in storage buffer were concentrated to between 5-10 mg/ml for use and tested for endotoxin with the EndoZyme recombinant Factor C assay (Hyglos GmbH). Endotoxin levels in all assays were maintained at < 0.1 EU/ml.
-
No products found
because this supplier's products are not listed.
Henriette Frikke-Schmidt, et al.,
bioRxiv - Physiology 2020
Quote:
... The plates for the islets were placed on a mat heated at 37 °C on a H101A ProScan motorized stage (Prior Scientific Instruments Ltd ...
-
No products found
because this supplier's products are not listed.
Alan P. R. Lorenzetti, et al.,
bioRxiv - Systems Biology 2022
Quote:
... Cell pellets were resuspended in Milli-Q water and disrupted at 4°C using ceramic beads (Mo Bio Laboratories) and a Precellys 24 homogenizer (Bertin Corp). Protein content was determined by bicinchoninic acid assay (BCA ...
-
No products found
because this supplier's products are not listed.
W. Coles Keeter, et al.,
bioRxiv - Immunology 2023
Quote:
... 8-12-week-old male mice were placed on a high fat diet with 0.15% added cholesterol (HFD-C, fat: 60% kcal; carbohydrate: 26% kcal, Bio-Serv F3282) for 28 weeks until sacrifice.
-
No products found
because this supplier's products are not listed.
Cameron Durfee, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... They were then baked at 65°C for 20 minutes to deparaffinize and then rehydrated with three consecutive washes in CitriSolv (Decon Labs) for 5 min each followed by graded alcohols as above ...
-
No products found
Tao Cheng, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 15 minutes at 4°C) from blood isolated by sub-mandibular bleeding and used for the BUN assay (BUN-Urea, BioAssay Systems) following the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Philippe Dehio, et al.,
bioRxiv - Cell Biology 2024
Quote:
... The slides were first plasma cleaned and PEG-coated at 4 °C overnight with 200µg/ml poly(L-lysine)-PEG (SuSoS, PLL(20)-g[3.5]-PEG(2) ...
-
No products found
because this supplier's products are not listed.
Lauren Rylaarsdam, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Organoid pieces were incubated for 75 minutes on a 95 RPM orbital shaker in a 37°C incubator and triturated using wide bore tips (Rainin, 30389218) every 30 minutes to help break up pieces ...
-
No products found
because this supplier's products are not listed.
Stijn Mouton, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... the overnight cultures were used both to create glycerol stocks for long-term storage at -80°C and for plasmid DNA extractions using the Plasmid DNA Extraction Mini Kit (Favorgen®). The extracted plasmids were used for Sanger sequencing using a universal T444T forward sequencing primer (Table S1).
-
No products found
because this supplier's products are not listed.
Chloe C. Ence, et al.,
bioRxiv - Microbiology 2024
Quote:
... The cultures were incubated at 37°C with 5% CO2 and routinely checked for mycoplasma contamination using the e-Myco Plus kit from Intron Biotechnology. Human hepatocellular carcinoma cells (HepG2 ...
-
No products found
because this supplier's products are not listed.
Sarah Mondoloni, et al.,
bioRxiv - Neuroscience 2022
Quote:
... slices (250 μm thick) were cut in SB-aCSF at 0-4°C using a Compresstome slicer (VF-200, Precisionary Instruments Inc.). Slices were then transferred to the same solution at 35°C for 10 min ...
-
No products found
because this supplier's products are not listed.
Thekla Cordes, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The cell suspension was centrifuged at 20,000 g for 10 min at 4 °C and 20 µl of the supernatant was used for protein quantification using BCA protein assay kit (Cat. #G1002, Lamda Biotech. Inc) as per manufacturer’s instruction.
-
No products found
because this supplier's products are not listed.
Abdoulie O. Touray, et al.,
bioRxiv - Microbiology 2023
Quote:
... Membranes were probed for 2 h at RT (or overnight at 4°C) with mAb α-V5 (BioShop Canada Inc., catalog number TAG006.100) 1:2,500 in 6% milk in PBS 0.05% Tween (PBS-T) ...
-
No products found
because this supplier's products are not listed.
M Sadeq Saleh, et al.,
bioRxiv - Neuroscience 2019
Quote:
Gold electroplating was carried out using in Earthcoat Cyanide Free Gold Plating Solution (24K) heated to 60 °C on a hot plate with stirrer (Model MS300HS, Medline Scientific, Oxon, United Kingdom). The solution (filled to an adequate depth for submersion in a 1 L thick glass beaker ...
-
No products found
because this supplier's products are not listed.
Bryce A. Jones, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... Litter-to-litter variation was controlled for by balancing treatment groups across litters. Nicotinamide riboside (CAS No. 1341-23-7) was obtained from ChromaDex (Los Angeles, CA) through participation in their External Research Program ...
-
No products found
because this supplier's products are not listed.
Dominik Schäfer, et al.,
bioRxiv - Bioengineering 2019
Quote:
... Hydrolysis was carried out at 180 rpm min−1 and 30°C for 138 h in sterile and closed 250 ml glass bottles (DWK Life Sciences GmbH, Mainz, Germany) to prevent evaporation and contamination ...