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No products found
because this supplier's products are not listed.
Andrea Orús-Alcalde, Aina Børve, Andreas Hejnol,
bioRxiv - Immunology 2022
Quote:
... and 1-bromo-3-chloropropane (Sigma). cDNA was synthetized using SuperScript™ III First-Strand Synthesis System (Invitrogen) ...
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No products found
because this supplier's products are not listed.
Clara R. Stelman, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 5-Bromo-4-chloro-3-indolyl phosphate (BCIP, [Roche]) and nitro blue tetrazolium chloride (NBT ...
-
No products found
because this supplier's products are not listed.
Hanlin Zhang, et al.,
bioRxiv - Immunology 2024
Quote:
... and 3 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) anti-CD28 (MA110172, Thermo Fisher), and cultured in the pre-coated plate for 3 days ...
-
No products found
because this supplier's products are not listed.
Jaakko Haverinen, Minna Hassinen, Matti Vornanen,
bioRxiv - Physiology 2021
Quote:
... L-364,373 (R-L3 or 5-(2-Fluorophenyl)-1,3-dihydro-3-(1H-indol-3-ylmethyl)-1-methyl-2H-1,4-benzodiazepin-2-one) (Tocris Cookson; Bristol, UK) and mefenamic (dimethylphenylaminobenzoic ...
-
No products found
because this supplier's products are not listed.
John Ong, et al.,
bioRxiv - Bioengineering 2024
Quote:
... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
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No products found
because this supplier's products are not listed.
Krzysztof Mikołajczyk,
bioRxiv - Biochemistry 2024
Quote:
... and untransfected cells were cultured for 2 weeks in a complete medium containing 3 μM N-[(1R,2R)-1- (2,3-dihydro-1,4-benzodioxin-6-yl)-1-hydroxy-3-pyrrolidin-1-ylpropan-2-yl]nonanamide (Genz-123346) (Merck, Darmstadt, Germany), a glucosylceramide synthase inhibitor [26].
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No products found
because this supplier's products are not listed.
Matthew A. Jorgenson, Joseph C. Bryant,
bioRxiv - Microbiology 2020
Quote:
The mutant library was screened by preparing a 1 × 10-6 dilution in LB medium and plating on LB agar containing 60 µg ml-1 X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Abcam) and 500 µM IPTG ...
-
No products found
because this supplier's products are not listed.
Toshiaki Mishima, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 14-3-3 (H-8; Santa Cruz Biotechnology), MARK3 (G-7 ...
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No products found
because this supplier's products are not listed.
Yu Sugihara, et al.,
bioRxiv - Plant Biology 2022
Quote:
... and histidine (H) and containing 5-Bromo-4-Chloro-3-Indolyl α-D-galactopyranoside (X-α-gal) (Clontech) to detect interactions ...
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No products found
because this supplier's products are not listed.
Erica W. Carter, Orlene Guerra Peraza, Nian Wang,
bioRxiv - Systems Biology 2023
Quote:
... Each row of a 96-well plate is a single BD construct (For example, row A: BD construct #1, row B: BD construct #2, row C: BD construct #3… to row H: BD construct #8) and each column is a single AD construct (For example column 1 ...
-
No products found
because this supplier's products are not listed.
Saori Shinoda, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′-bisphosphate) (18:1 PI(3,4)P2) (850153P; Avanti Polar Lipids) dissolved in chloroform to 1 µM final concentrations were mixed in a glass tube at the indicated ratios in the figure legends ...
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No products found
because this supplier's products are not listed.
Magdalena A. Sutcliffe, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... and the NEBNext Multiplex Oligos from Illumina (Index Primers Set 1, 2, 3 and 4, NEB, E7335S, E7500S, E7710S, E7730S), following the manufacturer’s protocols ...
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No products found
because this supplier's products are not listed.
Sarah M Bernhardt, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... or Cleaved Caspase-3 (CC3; Cell Signaling, 1:150, 1 h at room temperature). Slides were washed thrice in TBST prior to incubation with a pre-diluted HRP-conjugated secondary antibody (Nacalai USA Histofine) ...
-
No products found
because this supplier's products are not listed.
Yun Deng, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... with 5-bromo-4-chloro-3-indolyl-phosphate nitro blue tetrazolium staining (Vector Laboratories, Burlingame, CA, USA). 10 ∼ 30 embryos were used for each probe ...
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No products found
because this supplier's products are not listed.
Melanie Werner-Klein, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Alkaline phosphatase was developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitroblue tetrazolium (BCIP/NBT; BioRad, Germany) as substrate ...
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No products found
because this supplier's products are not listed.
Wenchao Li, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... SUMO 2/3 (Proteintech, cat. 11251-1-ap, 1:1000 for WB); Ubiquitin (Proteintech ...
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No products found
because this supplier's products are not listed.
Kim Doyon-Laliberté, et al.,
bioRxiv - Immunology 2022
Quote:
... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
-
No products found
because this supplier's products are not listed.
Meghan Robinson, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 0.5 mM RA and 1 mM 8-bromo-cAMP (Peprotech, 2354843) were added ...
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No products found
because this supplier's products are not listed.
Anthony Gurunian, Keren Lasker, Ashok A. Deniz,
bioRxiv - Biophysics 2024
Quote:
... 4-[2-[6-(dioctylamino)-2-naphthalenyl]ethenyl]-1-(3-sulfopropyl)-pyridinium (di-8-anepps) was purchased from Cayman Chemical (Ann Arbor, Michigan). Mineral Oil (USP grade ...
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No products found
because this supplier's products are not listed.
Subhash B. Arya, et al.,
bioRxiv - Immunology 2024
Quote:
Neutrophils migrating under the agarose for 1 h in 8 well chamber dishes were fixed using 4% paraformaldehyde (PFA, EMS #) in PHEM buffer for 20 min at 37 °C followed by agarose removal from the wells ...
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No products found
because this supplier's products are not listed.
Aleksandra J. Wierzba, et al.,
bioRxiv - Biochemistry 2024
Quote:
... or (1/8)NORAD-4xenv8- FL-3’antiPNA (Addgene plasmid #199209). For all experiments ...
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No products found
because this supplier's products are not listed.
Anthony Yan-Tang Wu, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 4°C for 1 h with MLS-55 rotor (Beckman Coulter). The pellets were lysed in 40 μL of radioimmunoprecipitation (RIPA ...
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No products found
because this supplier's products are not listed.
Annika Ullrich, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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No products found
because this supplier's products are not listed.
Päivi Ylä-Anttila, Maria G. Masucci,
bioRxiv - Cell Biology 2021
Quote:
... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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No products found
because this supplier's products are not listed.
Lisanne de Vor, et al.,
bioRxiv - Microbiology 2021
Quote:
... samples were further statically incubated for 1 h at 4°C with APC-conjugated polyclonal goat-anti-human IgG F(ab’)2 antibody (Jackson immunoresearch, 1:500). Fab fragments were detected with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech ...
-
No products found
because this supplier's products are not listed.
Sourabh Soni, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Total RNA was isolated at 6 time points (0, 0.5, 1, 2, 4, and 8 hours) using the RNeasy Mini kit (Qiagen) according to the manufacturer’s recommended protocol ...
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No products found
because this supplier's products are not listed.
Sara F. Costa, et al.,
bioRxiv - Microbiology 2023
Quote:
... with 100 μg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, VWR), or with 0.1 μM CdCl2 (Sigma-Aldrich) ...
-
No products found
because this supplier's products are not listed.
Wyatt E. Lanik, et al.,
bioRxiv - Cell Biology 2020
Quote:
... with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Corning) supplemented with 10% heat inactivated fetal bovine serum (Sigma) ...
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No products found
because this supplier's products are not listed.
Icaro Putinhon Caruso, et al.,
bioRxiv - Biophysics 2021
Quote:
... Samples of 20 μΜ N-NTD or N-NTD-SR consisting of varied protein:nucleic acid molar ratios (8:1, 4:1, 2:1, 1:1, 1:2) were prepared in low-binding microtubes (Eppendorf® LoBind) in the presence of 10% PEG-4000 (w/v ...
-
No products found
because this supplier's products are not listed.
Laust Bavnhøj, et al.,
bioRxiv - Plant Biology 2020
Quote:
... For all assays 1 μCi [3 H]-D-glucose (PerkinElmer, USA) was used as the radioactive tracer ...
-
No products found
because this supplier's products are not listed.
Larissa D. Kruse, et al.,
bioRxiv - Cell Biology 2024
Quote:
... cells were seeded on the fibronectin-coated Petri dish (TPP) and kept in the incubator (37 °C and 5% CO2) for 4-8 h in the EGM-2 (Lonza) containing 2% FBS ...
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No products found
because this supplier's products are not listed.
Huixia Ren, et al.,
bioRxiv - Systems Biology 2024
Quote:
... The samples were incubated for 2 h at 4℃ in a guinea pig anti-insulin antibody (1:200, A0564, Dako), a rabbit anti-somatostatin antibody (1:500 ...
-
No products found
because this supplier's products are not listed.
Susanne Klute, et al.,
bioRxiv - Microbiology 2024
Quote:
... the cells were stained with the following primary antibodies diluted in DPBS with 1% FBS for 2 h at 4 °C: Monoclonal mouse anti-StrepII-tag (1:200; Novus Biologicals, #NBP2-43735), monoclonal rabbit anti-LAMP1 (1:200 ...
-
No products found
because this supplier's products are not listed.
Elisabeth Seebach, et al.,
bioRxiv - Immunology 2023
Quote:
... the cells were pre-incubated with the respective antagonist as suggested by the manufacturer’s protocol (STING: H-151 for 1-2 hours and cGAS: RU.521 for 3 hours, both InvivoGen, USA) in cell growth medium ...
-
No products found
because this supplier's products are not listed.
Lucie Peskova, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... dissolved in a mixture of CUBIC-2 and PBS (3:1) on the bottom of Ibidi μ-Slide 8 well dishes (80826, Ibidi) and imaged on a confocal microscope ZEISS LSM 880 using 10x objective ...
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No products found
because this supplier's products are not listed.
Colin Reveley, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Then the section was incubated for 60 h at 4°C with a 1:200 dilution of a rabbit anti MAP-2 primary antibody (188 003, Synaptic Systems, Germany) and for 3 h with a 1:250 dilution of a goat anti-rabbit secondary antibody conjugated to DyLight 680 fluorophore (35569 ...
-
No products found
because this supplier's products are not listed.
Megha Sehgal, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Adult (3–8 months old) male and female Thy1-YFP-H mice (Jackson Laboratories, Stock No: 003782) were used for structural imaging experiments ...
-
No products found
because this supplier's products are not listed.
Wenyu Zhang, et al.,
bioRxiv - Microbiology 2023
Quote:
... anti-IL-8/IL-8 (MAB208, R&D Systems, 1:100), anti-CD45 (2403794 ...
-
No products found
because this supplier's products are not listed.
Anthony J. Snyder, Andrew T. Abad, Pranav Danthi,
bioRxiv - Microbiology 2021
Quote:
... The purified PCR products were then processed and sequenced using the NextSeq 75 – High Output (82 cycles in read 1, 8 cycles in index 1, and 8 cycles in index 2 SE reads) (Illumina). The sequencing data was analyzed using the Model-Based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK ...
-
No products found
because this supplier's products are not listed.
Jiefu Wang, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Tissues were fixed with 4% PFA for 1 h prior to vibratome (Leica) sectioning at 100 μm ...
-
No products found
because this supplier's products are not listed.
Sophie Dürauer, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and cell numbers were recorded every 8 h for 3 days using Cytation 5 (BioTek) equipped with a 4x objective and the Gen5 software (ver ...
-
No products found
because this supplier's products are not listed.
Biren M. Dave, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, cat# 05790), 1X N2 ...
-
No products found
because this supplier's products are not listed.
Christophe Royer, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 3 to 4 week old CD-1 females (Charles River UK) were injected intraperitoneally with 5 IU of PMSG (Intervet ...
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No products found
because this supplier's products are not listed.
Leticia Peris, et al.,
bioRxiv - Neuroscience 2021
Quote:
... at 4°C overnight and 1 h with appropriate secondary antibodies (LI-COR Biosciences). Image acquisition was performed with an Odyssey infrared imaging system (LI-COR Biosciences ...
-
No products found
because this supplier's products are not listed.
Rianna Vandergaast, et al.,
bioRxiv - Immunology 2020
Quote:
... and incubated for 1 h at room temperature with primary antibody mouse α-SARS-CoV-2 Spike (1:1000, GeneTex #GTX632604) or rabbit polyclonal α-VSV (1:5000 ...
-
No products found
because this supplier's products are not listed.
Sara Lago, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... pRPA32 1:1000 (Bethyl, A300-246A-8) and 53BP1 1:100 (Millipore ...
-
No products found
because this supplier's products are not listed.
Qiufeng Liu, et al.,
bioRxiv - Biophysics 2021
Quote:
... CCKAR–G protein complexes were assembled at room temperature (RT) for 1 h by the addition of 10 μM CCK-8 (GenScript) and 25 mU/mL apyrase ...
-
No products found
because this supplier's products are not listed.
Zachary Beine, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The primary antibody (2-3% serum, 0.4% PBST, 1:500 Rockland 600-401-379) was applied and incubated for ninety minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Jonathan C. Brown, et al.,
bioRxiv - Microbiology 2021
Quote:
... Cells were treated with methanol 0.6% H2O2 and stained for 1 h with a 1:3000 dilution of 40143-R019 rabbit mAb to SARS-CoV-2 nucleocapsid protein (Sino Biological). A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma ...
-
No products found
because this supplier's products are not listed.
Wenjian Lv, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...