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1 - 50 of 1437 citations for 8' Bromo 2' 3' dihydro 1'h spiro cyclopropane 1 4' isoquinoline since 2020

• SARS-CoV-2 Omicron Envelope T9I adaptation confers resistance to autophagy

  Susanne Klute, et al., bioRxiv - Microbiology 2024

  Quote: ... the cells were stained with the following primary antibodies diluted in DPBS with 1% FBS for 2 h at 4 °C: Monoclonal mouse anti-StrepII-tag (1:200; Novus Biologicals, #NBP2-43735), monoclonal rabbit anti-LAMP1 (1:200 ...
• SNRK regulates TGFβ levels in atria to control cardiac fibrosis

  Karthikeyan Thirugnanam, et al., bioRxiv - Cell Biology 2024

  Quote: ... Smad 2/3 (Novus Biologicals, Cat# AF3797, 1:200 dilution), GAPDH (Santa Cruz ...
• Chromosome Integrity is Required for the Initiation of Meiotic Sex Chromosome Inactivation in Caenorhabditis elegans

  Yisrael Rappaport, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-HIM-8 (Novus Biological, 1:2000), rat anti-HIM-8 (1:100 ...
• Quantitative Cytogenetics Reveals Molecular Stoichiometry and Longitudinal Organization of Meiotic Chromosome Axes and Loops

  Alexander Woglar, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-REC-8 (1:10000) (Novus Biologicals), rabbit anti-HIM-3 (1:200 ...
• Condensin II activation by M18BP1

  Alessandro Borsellini, et al., bioRxiv - Cell Biology 2024

  Quote: ... CAP- H (NBP1-32573, Novus Biologicals, 1:1000), CAP-H2 (A302-275A ...
• The distance between the plasma membrane and the actomyosin cortex acts as a nanogate to control cell surface mechanics

  Sergio Lembo, et al., bioRxiv - Biophysics 2023

  Quote: ... The blot was blocked in 5% BSA in TBST and subsequently incubated with primary antibody (anti-mDia1, #20624-1-AP, Thermo Fischer Scientific, 1:1000, overnight at 4° C; GAPDH, #NB300-221, Novus Biologicals, 1:40.000, 1-2 hrs at RT). Secondary antibody staining was performed for 1 hour at RT in blocking solution (Donkey-Anti-Rabbit-HRP ...
• Inflammation-mediated Upregulation of VCAM-1 but not KIM-1 during Acute Kidney Injury to Chronic Kidney Disease Transition

  Isabel Melchinger, et al., bioRxiv - Pathology 2022

  Quote: ... Membrane was blocked with 5% non-fat milk in TBST for 2 hrs and probed overnight at 4°C with the primary antibody against TIM-1/KIM-1/HAVCR (#AF1817, Novus Biologicals). After washing the membrane with TBST ...
• PARG establishes a functional module with BRCA1-BARD1 that controls DNA repair pathway choice during gametogenesis

  Shalini Trivedi, Jitka Blazicková, Nicola Silva, bioRxiv - Genetics 2022

  Quote: ... rabbit anti-HIM-8 1:1000 (Novus Biologicals, # 41980002). All secondary antibodies were Alexa Fluor conjugates to 488 ...
• Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb

  Jane S. Huang, et al., bioRxiv - Neuroscience 2022

  Quote: ... were stained for GAP43 as a marker of immature OSNs (anti-GAP43 primary antibody, 1:1000 for 48 h at 4 °C, NB300-143, Novus Biologicals; donkey anti-rabbit-Alexa Fluor 546 secondary antibody ...
• Protective threshold of a potent neutralizing Zika virus monoclonal antibody in rhesus macaques

  Joseph P. Nkolola, et al., bioRxiv - Microbiology 2024

  Quote: ... ELISA plates were coated overnight at 4°C with 1 μg/mL of goat anti-human IgG (H+L) secondary antibody (monkey pre-adsorbed) (Novus Biologicals) and then blocked for 2 hours ...
• Enteropathogenic Escherichia coli (EPEC) Infection Induces Diarrhea, Intestinal Damage, Metabolic Alterations and Increased Intestinal Permeability in a Murine Model

  Solanka E. Ledwaba, et al., bioRxiv - Microbiology 2020

  Quote: ... 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600; rabbit anti-claudin-1, 1:1000, Novus biological, NBP1-77036) and secondary antibodies (Cy3-conjugated AffiniPure donkey anti-rabbit ...
• Computer Designed PRC2 Inhibitor, EBdCas9, Reveals Functional TATA boxes in Distal Promoter Regions

  Shiri Levy, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Oct 4 (Novus Biologicals, NB11- 1:150), P16 (Invitrogen MA5-14260 ...
• Mimicking the breast metastatic microenvironment: characterization of a novel syngeneic model of HER2⁺ breast cancer

  Aaron G. Baugh, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-AE-1/AE-3 (1:200 dilution; Novus Biologicals, cat. NBP2-29429). Slides were then incubated with HRP-conjugated anti-rabbit secondary antibody (Leica Microsystems ...
• Stabilization of GTSE1 by cyclin D1-CDK4/6 promotes cell proliferation: relevance in cancer prognosis

  Nelson García-Vázquez, et al., bioRxiv - Biochemistry 2024

  Quote: ... LC-3 (1:5,000, Novus Biological NB100–2220), p21 (1:1,000 ...
• Shared and divergent phase separation and aggregation properties of brain-expressed ubiquilins

  Julia E. Gerson, et al., bioRxiv - Neuroscience 2020

  Quote: ... Membranes were then probed overnight at 4℃ in anti-Ubiquilin-2 (Novus Biologicals, cat. #NBP2-25164; 1:2000), anti-Ubiquilin-1 (Novus biologicals ...
• SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta

  Lissenya B. Argueta, et al., bioRxiv - Cell Biology 2021

  Quote: ... for 1 hour at room temperature and then incubated overnight at 4°C in a humid chamber with primary antibodies (SARS-CoV-2-N, GeneTex GTX635679, at 1:100; CD163,Novus Biologicals NBP2-48846, 1:250) diluted in 1% BSA/0.1% Triton-X PBS (PBST) ...
• Retrograde tracing of breast cancer-associated sensory neurons

  Svetllana Kallogjerovic, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by overnight incubation (PMSNs) or 96 h (DRGs) at 4°C with the mouse monoclonal anti-CGRP (1:200, Novus Biologicals, Cat# NB036540), chicken polyclonal to Neurofilament heavy polypeptide NF200 (1:250 ...
• Cerebral Organoids In Primary Progressive Multiple Sclerosis Reveal Stem Cell Disruption And Failure To Produce Oligodendrocytes

  Nicolas Daviaud, et al., bioRxiv - Neuroscience 2022

  Quote: ... Slides were then incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: rabbit anti-cleaved caspase 3 (1:200, Novus Biologicals), rat anti-CTIP2 (1:500 ...
• Skp1 proteins are structural components of the synaptonemal complex in C. elegans

  Joshua Blundon, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-HIM-3 (rabbit polyclonal, 1:200 or 1:100; Novus Biologicals #53470002) or anti HTP-3 (chicken polyclonal ...
• Python nidoviruses, more than respiratory pathogens

  Eva Dervas, et al., bioRxiv - Pathology 2020

  Quote: ... followed by a 15 min incubation with DAPI (4′, 6-diamidino-2-phenylindole, Novus Biologicals; 1:10,000 in PBS). Sections were washed twice with distilled water ...
• Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation

  Allison K. Meyers, et al., bioRxiv - Immunology 2021

  Quote: ... LC-3 (Novus Biologicals, no. NB100-2220; 1:500), P62 (Novus Biologicals ...
• Met/HGFR triggers detrimental reactive microglia in TBI

  Rida Rehman, et al., bioRxiv - Neuroscience 2021

  Quote: ... Mouse anti-Integrin beta 3 (1:50, Novus biologicals), Guinea pig anti-Iba1 (1:500 ...
• Disruption of augmin-mediated microtubule nucleation in neural stem cells causes p53-dependent apoptosis and aborts brain development

  Ricardo Viais, et al., bioRxiv - Cell Biology 2020

  Quote: ... cleaved caspase-3 (Novus Biologicals #MAB835, dilution 1:500), γ-tubulin (ExBio ...
• TFAP2C and HNRNPK control mTOR cell metabolism and prion propagation

  Stefano Sellitto, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-Caspase 3 1:1000 (Novus Biologicals, NB100-56708), anti-GPX4 1:1000 (ab125066) ...
• Inhibitory concentrations of ciprofloxacin induce an adaptive response promoting the intracellular survival of Salmonella Typhimurium

  Sushmita Sridhar, et al., bioRxiv - Microbiology 2021

  Quote: ... then for 1 h with CSA-Alexa-647 (Novus Biologicals, NB110-16952AF647) at 1:1000 in BSA ...
• Computer Designed PRC2 Inhibitor, EBdCas9, Reveals Functional TATA boxes in Distal Promoter Regions

  Shiri Levy, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Oct-4 (Santa Cruz sc-5279, 1:1000, Novus Biologicals NB110-90606, 1:500), H3K27me3 (Active Motive 39155 1:1000) ...
• SNRK regulates TGFβ levels in atria to control cardiac fibrosis

  Karthikeyan Thirugnanam, et al., bioRxiv - Cell Biology 2024

  Quote: ... for 15 min and blocked with 4% BSA in PBS for 1 h and overnight incubation of α-SMA antibody (Novus biologicals, Cat#NB300-978) diluted (1:1000 ...
• Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

  Maria K. Lagou, et al., bioRxiv - Immunology 2024

  Quote: ... Nuclear counterstaining was performed for 5min with 4′,6-diamidino-2-phenylindole (DAPI, dilution 1:1000 in PBST 0,05%; Novus Biologicals), or with Deep Red Anthraquinone 5 (DRAQ5 ...
• Identification of novel myokines and putative protein targets that mediate functional adaptations in response to chronic contractile activity induced skeletal muscle-extracellular vesicle treatment

  Patience O. Obi, et al., bioRxiv - Cell Biology 2024

  Quote: ... mouse anti-ATP7A (S60-4, Novus Biologicals, 1:200), rabbit anti-ITGA6 (3750 ...
• Myosin-1b interacts with UNC45A and controls intestinal epithelial morphogenesis

  Céline Revenu, et al., bioRxiv - Developmental Biology 2021

  Quote: ... Sections were incubated for 30 min at room temperature in blocking buffer (3% BSA in PBS) and then overnight at 4°C with anti Myo1b antibody (1:200, Novus Biologicals NBP1-87739) in blocking ...
• 3D microarchitecture of the human tuberculous granuloma

  Gordon Wells, et al., bioRxiv - Microbiology 2020

  Quote: ... 3-mercaptopyruvate sulfurtransferase (MPST; NBP1-82617, Novus Biologicals, 1:100), CD68 (M0814-CD68-KP1,DAKO,1:3000) ...
• Condensate formation of the human RNA-binding protein SMAUG1 is controlled by its intrinsically disordered regions and interactions with 14-3-3 proteins

  John Fehilly, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-14-3-3ζ (Novus Biologicals, NBP2-24593SS, 1:1000). Following primary antibody incubation ...
• APOE4 alters the lipid droplet proteome and modulates droplet dynamics

  Cassi M. Friday, et al., bioRxiv - Physiology 2024

  Quote: ... The membranes were incubated overnight at 4° Celsius in 1:1000 PLIN-2 primary antibody solution (Novus Biologicals, Centennial, CO, USA). After incubation ...
• HDAC1 SUMOylation promotes Argonaute directed transcriptional silencing in C. elegans

  Heesun Kim, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-HPL-2 (1:1000) (Novus Biologicals, 38630002), anti-LIN-53 (1:1000 ...
• Misaligned chromosomes that satisfy the spindle assembly checkpoint are a strong predictor of micronuclei formation in dividing cancer cells

  Ana Margarida Gomes, et al., bioRxiv - Cell Biology 2022

  Quote: ... rabbit anti-Septin-2 (1:500; Novus Biologicals), rabbit anti-CEP90/PIBF1 (1:1000 ...
• GASOTRANSMITTER MODULATION OF HYPOGLOSSAL MOTONEURON ACTIVITY

  Brigitte Browe, et al., bioRxiv - Neuroscience 2022

  Quote: ... HO-2 (1:200, Novus Biologicals; # NBP1-52849) and CSE (1:250 ...
• Microfluidic organotypic device to test intestinal mucosal barrier permeability ex vivo

  Amanda E Cherwin, et al., bioRxiv - Bioengineering 2023

  Quote: ... 1:500 and anti-MUC-2 (Novus Biologicals) at 3 μg/mL ...
• CHCHD2 mediates glioblastoma cell proliferation, mitochondrial metabolism, hypoxia-induced invasion, and therapeutic resistance

  Jan C. Lumibao, et al., bioRxiv - Cancer Biology 2022

  Quote: ... GPx-4 (1:100, NBP2-75511, Novus Biologicals, Centennial, CO), and matrix metalloproteinase 2 (MMP-2 ...
• RbAp46/48^LIN-53 and HAT-1 are required for initial CENP-A^HCP-3 deposition and de novo centromere formation in Caenorhabditis elegans embryos

  Zhongyang Lin, Karen Wing Yee Yuen, bioRxiv - Cell Biology 2020

  Quote: ... using rabbit (Rb)-anti-HCP-3 (1:1000; Novus biologicals 29540002), Rb-anti-KNL-2 (1:500 ...
• Disease-Associated Non-Coding Variants Alter NKX2-5 DNA-Binding Affinity

  Edwin G. Peña-Martínez, et al., bioRxiv - Biochemistry 2022

  Quote: ... Membranes were blocked using 5% milk in TBST buffer for 1 h in orbital shaking and incubated overnight with 1:10,000 dilution of Anti-His mouse monoclonal antibodies (Novus Biologicals, AD1.1.10). The SDS-PAGE and Western Blot Analysis results were imaged using Azure® Biosystems Imager.
• Cardiovascular Disease-Associated Non-Coding Variants Disrupt GATA4-DNA Binding and Regulatory Functions

  Edwin G. Peña-Martínez, et al., bioRxiv - Genomics 2024

  Quote: ... Membranes were blocked using 5 % milk in 1× TBST buffer for 1 h in orbital shaking and incubated overnight with 1:10,000 dilution of Anti-His mouse monoclonal antibody (Novus Biologicals, AD1.1.10). The SDS-PAGE and Western Blot Analysis results were imaged using Azure Sapphire Bio-molecular Imager (Azure Biosystems ...
• Resurrecting essential amino acid biosynthesis in a mammalian cell

  Julie Trolle, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... for 1 h at room temperature prior to incubation in primary antibody (Novus Biologicals, NBP2-59627 [1:1000 dilution] ...
• Single-nucleus and spatial transcriptomics of archival pancreatic cancer reveals multi-compartment reprogramming after neoadjuvant treatment

  William L. Hwang, et al., bioRxiv - Genomics 2020

  Quote: ... 1:40 anti-panCK-Alexa Fluor 532 (clone AE-1/AE-3; Novus Biologicals, cat. no. NBP2-33200AF532), 1:40 anti-CD45-Alexa Fluor 594 (clone 2B11 + PD7/26 ...
• Enhanced Complement Expression in the Tumor Microenvironment Following Neoadjuvant Therapy: Implications for Immunomodulation and Survival in Pancreatic Ductal Adenocarcinoma

  Xiaofei Zhang, et al., bioRxiv - Genomics 2023

  Quote: ... 1:20 anti-panCK-Alexa Fluor 532 (clone AE-1/AE-3; Novus Biologicals, cat. no. NBP2-33200AF532), and 1:100 anti-αSMA-Alexa Fluor 647 (clone 1A4 ...
• Nose-only Exposure to Cherry and Tobacco Flavored E-cigarettes Induced Lung Inflammation in Mice in a Sex-dependent Manner

  Thomas Lamb, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... Ly6B.2 (Novus Biological Cat # NBP2-13077, 1:250), CD4 (Invitrogen Cat # 25-0041-82 ...
• Nuclear envelope assembly relies on CHMP-7 in the absence of BAF-LEM-mediated hole closure

  Sarah R. Barger, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1 μg/mL rabbit α-LEM-2 (Novus Biologicals), 1 μg/mL rabbit α-CHMP-7 (Shankar et al. ...
• Differentiation of Peritubular Myoid-Like Cells from Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 Beta- And Steroid Delta-Isomerase 1 (HSD3β, Novus Biologicals, NB110-7844) 1:200 ...
• A Novel Organoid Model of In Vitro Spermatogenesis Using Human Induced Pluripotent Stem Cells

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 Beta- And Steroid Delta-Isomerase 1 (HSD3β, Novus Biologicals, NB110-7844) 1:200 ...
• Microfluidic bioprinting for the in vitro generation of novel biomimetic human testicular tissues

  Meghan Robinson, et al., bioRxiv - Bioengineering 2021

  Quote: ... 3 Beta- And Steroid Delta-Isomerase 1 (HSD3β, Novus Biologicals, NB110-7844) 1:200 ...
• Human TRMT2A methylates tRNA and contributes to translation fidelity

  Monika Witzenberger, et al., bioRxiv - Biochemistry 2023

  Quote: ... Primary antibody used: mouse anti-BirA (1:10; Novus Biologicals, #5B11c3-3). Secondary antibody used ...