-
No products found
because this supplier's products are not listed.
Amin Zargar, et al.,
bioRxiv - Synthetic Biology 2020
Quote:
... 5-methyl-6-(propan-2-yl)oxan-2-one was synthesized by Enamine (Cincinnati, USA) to greater than 95% purity.
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6-Chloro-7-hydroxy-4-methylcoumarin is a pharmaceutical intermediate.
Cat# S5760, SKU# S5760-5mg,
5mg, $107.00
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Liying Chen, et al.,
bioRxiv - Neuroscience 2023
Quote:
The D1 receptor antagonist R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepi ne hydrochloride (SCH-23390, Selleck chemicals, China) was dissolved in 0.9% saline and stored -80°C ...
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No products found
because this supplier's products are not listed.
Miquel Sendra, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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No products found
because this supplier's products are not listed.
Shaibu Oricha Bello, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... Neutral red (3-amino-7-dimethylamino-2-methyl-phenazine hydrochloride) (Solarbio, cat. N8160), Minimal Essential Medium/Earls Balance Salts (MEM/EBSS ...
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No products found
because this supplier's products are not listed.
Yohan S.S. Auguste, et al.,
bioRxiv - Neuroscience 2022
Quote:
... subcutaneous] before being anesthetized using isoflurane (SomnoSuite, Kent Scientific; 3-5% induction, 1-2% maintenance). Once anesthetic depth was achieved ...
-
No products found
because this supplier's products are not listed.
Tulsi Upadhyay, Vaibhav V Karekar, Ishu Saraogi,
bioRxiv - Biochemistry 2020
Quote:
... All GrpE variants were labeled with DACM (N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide) (AnaSpec) and DnaK was labeled with BODIPY-fluorescein-N-(2-aminoethyl)-maleimide (Invitrogen ...
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No products found
because this supplier's products are not listed.
Urszula Kozłowska, et al.,
bioRxiv - Cell Biology 2021
Quote:
... (TLR4, Bioss, 1:200, overnight, +4□C), (CD11b ...
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No products found
because this supplier's products are not listed.
Jennifer Schwarz, et al.,
bioRxiv - Biochemistry 2021
Quote:
... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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No products found
because this supplier's products are not listed.
Wei-Sheng Sun, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 4°C and incubated at 4 °C with Ni-NTA (Macherey-Nagel). The Ni-NTA column was washed with 10 column volumes of 20 mM HEPES/NaOH (pH 7.0) ...
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No products found
because this supplier's products are not listed.
Garrett D. Sheehan, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Slides were next gently rocked in a humidity chamber overnight at 4° C with Blocking Buffer containing either Mouse anti-Magi-2 (1:250, Abnova), Guinea Pig anti-PKCγ (1:500 ...
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No products found
because this supplier's products are not listed.
Aakash Basu, et al.,
bioRxiv - Neuroscience 2022
Quote:
... mice were first anaesthetized with isoflurane in oxygen (3-5% during induction, slowly lowered throughout the surgery to 1-2%) while placed in a stereotaxic apparatus (Stoelting). Eyes were lubricated with ophthalmic ointment ...
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No products found
because this supplier's products are not listed.
Matthias Fellner, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Caspase 3-like activity was measured fluorometrically by the addition of 50 µM acetyl-Asp-Glu-Val-Asp-7-amino-4-methylcoumarin (Ac-DEVD-AMC) at 37 °C in 96-well plates using a ClarioSTAR microplate reader (BMG LABTECH). The production of AMC (λEM = 390 nm ...
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No products found
because this supplier's products are not listed.
Daniel da Silva e Silva, et al.,
bioRxiv - Neuroscience 2022
Quote:
... post-fixed for 2 h in 4% PFA at 4°C and immediately sectioned in coronal slices (50 μm) using a vibratome (Ted Pella). Coronal sections were incubated with 10 mM sodium citrate (pH 6.0 ...
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No products found
because this supplier's products are not listed.
Alison Besse, et al.,
bioRxiv - Microbiology 2022
Quote:
... at 37°C in a TC-7 roller drum (New Brunswick) at 240 rpm for 16 h ...
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No products found
because this supplier's products are not listed.
Reza Nejat, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
Z-RLRGG-7-amino-4-methyl-courmarin (peptide-AMC) was purchased from Bachem. Ubiquitin−7-amino-4-methylcourmarin (Ub-AMC ...
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No products found
because this supplier's products are not listed.
Melody Li, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Patch pipettes of 3-5 MΩ (Harvard Apparatus) were made using a puller (P-1000 ...
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No products found
because this supplier's products are not listed.
Jared M. Fischer, et al.,
bioRxiv - Bioengineering 2024
Quote:
... plates were incubated at 37 °C under 5% CO2 for 2 hours before measuring the MTS absorbance at 490 nm using a microplate reader (TECAN Spark 20M).
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No products found
because this supplier's products are not listed.
Vivian K. Rojas, et al.,
bioRxiv - Microbiology 2024
Quote:
... The chromogenic molecule X-Phos (5-bromo-4-chloro-3-indolyl phosphate, Chem-Impex) was added to agar plates with the purpose of detecting S ...
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Building Block
Sold for research purposes only.
Cat# 2592.0, SKU# 2592-1000 mg,
1000mg, US $165.00 / EA, EURO, €150 / EA
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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No products found
because this supplier's products are not listed.
Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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No products found
because this supplier's products are not listed.
Subash Godar, et al.,
bioRxiv - Biophysics 2021
Quote:
... We detected the bound alkaline phosphatase labeled antibodies by incubating the membrane in BCIP/NBT (5-bromo, 4-chloro, 3- indolylphosphate/nitro-blue tetrazolium, AMRESCO, LLC.) substrate for 30–45 minutes ...
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No products found
because this supplier's products are not listed.
Martineau Éric, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Muscles were incubated overnight at 4°C with a rat IgG2a anti-MAC-2 (Galectin-3) antibody (1:250; clone M3/38; Cedarlane, cat# CL8942AP), then 2h at RT with a rabbit IgG anti-S100β antibody (1:250 ...
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No products found
because this supplier's products are not listed.
Helmut Bischof, et al.,
bioRxiv - Cancer Biology 2023
Quote:
Glucose uptake was assessed using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2- NBDG) (Biomol GmbH). Therefore ...
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No products found
because this supplier's products are not listed.
Juan Yang, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sulfo-Cyanine 3 Azide (2-5 uM final, Lumiprobe, #D1330), and fresh Sodium Ascorbate (100 mM final ...
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No products found
because this supplier's products are not listed.
W. Grant Ludlam, et al.,
bioRxiv - Biochemistry 2019
Quote:
The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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No products found
because this supplier's products are not listed.
H. Theobald, et al.,
bioRxiv - Immunology 2022
Quote:
... centrifuged at 1350 rpm for 5 min at 4°C and resuspended in 1 ml RPMI1640 (PAN Biotech) supplemented with 10% FCS (Sigma-Aldrich) ...
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No products found
because this supplier's products are not listed.
Emily A. Rex, et al.,
bioRxiv - Microbiology 2023
Quote:
... IP of total SUMOylated protein fractions used either SUMO-1 or SUMO-2/3 Ab included with the Signal Seeker SUMOylation 1 or 2/3 Detection Kit (Cytoskeleton) and IPs were carried out according to the manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Timothy N. Hoang, et al.,
bioRxiv - Immunology 2020
Quote:
... The sections were incubated with Thyroid Transcription Factor-1 (Clone 8G7G3/1) for overnight at 4°C followed by a detection polymer system (MACH 2™; Biocare Medical). Labeled antibody was visualized by development of the chromogen (DAB Chromogen Kits ...
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No products found
because this supplier's products are not listed.
Lindsey N. Block, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The blots were blocked at 4°C overnight in 5% nonfat dry milk (RPI, Cat # M17200 ...
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No products found
because this supplier's products are not listed.
Negm Ahmed, et al.,
bioRxiv - Neuroscience 2021
Quote:
... for 5 min at 4°C in Micro tube 1.1ml Z-Gel (Sarstedt, Germany). Samples were kept on ice at all times ...
-
No products found
because this supplier's products are not listed.
Shaohe Wang, Kazue Matsumoto, Kenneth M. Yamada,
bioRxiv - Developmental Biology 2020
Quote:
... 4 mL 5× PEG reagent (System Biosciences, LV825A-1) was added and mixed by pipetting ...
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No products found
because this supplier's products are not listed.
Megan B. Borror, et al.,
bioRxiv - Pathology 2019
Quote:
R-loop formation in worms was quantified using αS9.6 primary antibody (Kerafast, 1:2000 in 5% Blotto, 16 hours, 4°C), previously shown to be selective for DNA::RNA hybrids 50 ...
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No products found
because this supplier's products are not listed.
Uljana Kravchenko, et al.,
bioRxiv - Microbiology 2020
Quote:
... The fixed cells were harvested (8000 g, 5 min, 4 °C) and resuspended in 1 mL of ExtractRNA Reagent (Evrogen, Russia) and the subsequent procedures were performed according to manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Andrea Bernardini, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 1× PIC at 4°C using DiaEasy dialyzers (BioVision K1013-10) and protein concentration was measured using Bradford assay (BioRad ...
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No products found
because this supplier's products are not listed.
Wim J. de Jonge, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... on a heated stir plate (IKA C-MAG HS 7) set to 30°C and ~250 rpm ...
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No products found
because this supplier's products are not listed.
Caelan E Radford, et al.,
bioRxiv - Immunology 2023
Quote:
... 1 µL of 10 µM 3’ PacBio round 2 reverse primer (PacBio_3pri_RND2), and 25 µL KOD Hot Start Master Mix ...
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No products found
because this supplier's products are not listed.
Diones Bueno, et al.,
bioRxiv - Neuroscience 2023
Quote:
Denatured total cellular protein samples (95°C, 5 min) from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels ...
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No products found
because this supplier's products are not listed.
Brian T. Do, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... with a 4:2:1:1 ratio of the target:pMDLg:pMD2.G:pRSV-REV plasmids using Transit 293T reagent (Mirus). After 48 hours ...
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No products found
because this supplier's products are not listed.
Simon Schäper, et al.,
bioRxiv - Microbiology 2023
Quote:
... 5-bromo-4-chloro-3-indolyl β-d-galactopyranoside (X-Gal, Apollo Scientific) was used at 100 μg/ml ...
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No products found
because this supplier's products are not listed.
Grace I. Borlee, et al.,
bioRxiv - Microbiology 2021
Quote:
... supplemented with 100 nM of 2-chloro-adenosine-5’-O-monophsphate (2-Cl-5’-AMP, Axxora, LLC) for internal standardization ...
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No products found
because this supplier's products are not listed.
Andrew Barszczyk, et al.,
bioRxiv - Cell Biology 2023
Quote:
... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
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No products found
because this supplier's products are not listed.
Victoria A. Bonnell, et al.,
bioRxiv - Genomics 2023
Quote:
... Sonicate the chromatin until sufficiently sheared (130µL, 5% duty cycle, 75W peak incident power, 200 cycles per burst, 7°C, for 5 minutes using Covaris Focus-Ultrasonicator M220). The immunoprecipitation step included ...
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No products found
because this supplier's products are not listed.
Li Zhenyu, Li Tian, Liu Meisui, Ivanovic Tijana,
bioRxiv - Microbiology 2022
Quote:
... sn-(1-oleoyl-2-hydroxy)-glycerol-3-phospho-sn-3′-(1′-oleoyl-2’-hydroxy)-glycerol (ammonium salt) (18:1 BMP (R,R); Avanti Polar Lipids) ...
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No products found
because this supplier's products are not listed.
Camilla Schinner, et al.,
bioRxiv - Cell Biology 2021
Quote:
... The following primary antibodies were incubated in antibody buffer (Intercept blocking buffer diluted 1:1 in TBS containing 0.2 % tween 20) at 4 °C overnight: Mouse anti-DSG1/2 (61002, Progen, Heidelberg, Germany), mouse anti-DSP (61003 ...
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No products found
because this supplier's products are not listed.
Cherrelle Dacon, et al.,
bioRxiv - Immunology 2022
Quote:
... 7 μm streptavidin beads (Spherotech, SVP-60-5) coated in tandem with 10 μg/mL MERS-CoV spike and 10 μg/mL OC43-CoV spike were re-suspended in a cocktail of 2.5 μg/mL goat anti-human IgG-Alexa Fluor 647 (Jackson ImmunoResearch ...
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No products found
because this supplier's products are not listed.
Emilia Neuwirt, et al.,
bioRxiv - Immunology 2022
Quote:
... 3 - 5 μM MCC950 (Adipogen), 50 - 200 nM MG132 (Sigma) ...
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No products found
because this supplier's products are not listed.
Desirée Böck, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Brain sections were incubated with primary antibodies overnight at 4°C (rabbit-NeuN, 1:1’000, abcam 177487; mouse-TH; 1:1’000, Immunostar 22941 ...
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No products found
because this supplier's products are not listed.
F. Abrar, et al.,
bioRxiv - Neuroscience 2023
Quote:
... and heavy isotopes (4, 4, 5, 5-D4 L-lysine and 13C6 L-arginine, Cambridge Isotope Laboratories (CIL)) for the K4/R6 population ...
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No products found
because this supplier's products are not listed.
Qi Feng, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and incubated overnight at 4°C with primary antibodies against DCBLD1 (1:30, Atlas antibody, Bromma, Sweden) and CD31 (1:100 ...
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No products found
because this supplier's products are not listed.
Sahil Nagpal, et al.,
bioRxiv - Biophysics 2023
Quote:
U-2 OS and COS-7 cells were obtained from American Type Culture Collection, Manassas ...