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Citations for GenScript :
51 - 95 of 95 citations for ssc mir 369 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Capture antibodies: affinity purified rabbit anti-L1 (anti-ORF1p or anti-ORF2p (RT fragment)) polyclonal antibodies were ordered from GenScript (Custom Polyclonal Antibody Production Service) ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplifications were performed with Taq DNA Polymerase (GenScript) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Receptor was detected by primary rabbit anti-SNAP antibody (50 μL/well of 1:2000 dilution, GenScript, 1 h at RT) and anti-rabbit HRP antibody (50 μL/well of a 1:1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... except the coding sequence of TaDCX was amplified using primers S4 and AS4 using ptub-mEmeraldFP-TaDCX (synthesized by GenScript Inc, NJ) as the template.
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bioRxiv - Bioengineering 2020Quote: ... melanogaster U6:3 UTR that was amplified with primers 1045.C13 and 1045.C14 from a gene synthesized vector (GenScript, Piscataway, NJ) was cloned using EA cloning ...
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bioRxiv - Synthetic Biology 2020Quote: ... CCT isoforms were PCR amplified from synthetic vectors from GenScript. SpCas9 was PCR amplified from px4591 ...
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bioRxiv - Molecular Biology 2021Quote: ... Point mutations were introduced by the PCR CloneEZ method (GenScript). Plasmids were transformed in acrB-deficient MG1655 E ...
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bioRxiv - Plant Biology 2021Quote: ... The purified PCR amplicons were sequenced using Sanger sequencing (Genscript, NJ). The overlapping PCR amplicon sequences were aligned and comparative analysis with the WT sequences was used to identify mutations for each of the independent nec3 mutants.
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were generated by PCR or DNA synthesis (Genscript, New Jersey) containing ∼500-1000 bp DNA flanking each gene and fused together ...
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bioRxiv - Microbiology 2020Quote: ... hnRNPUL1 (NM_007040) and PEG10 (NM_015068.3) were PCR amplified from ORF clones (hnRNPL, GenScript #OHu14072 ...
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bioRxiv - Biochemistry 2020Quote: ... Templates for sgRNA transcription were generated by PCR amplifying synthesized fragments (IDT and Genscript) or by annealing a T7 primer oligo to a single stranded template oligonucleotide ...
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bioRxiv - Biochemistry 2024Quote: ... the coding sequences for the PINK1 constructs were PCR amplified using clone OHu25380D (Genscript) as a template and cloned into the vector pFB-6HZB (SGC ...
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bioRxiv - Biochemistry 2023Quote: ... the PCR amplified sfGFP sequence was first subcloned into vector pUC57 containing synthesized (GenScript) C-terminus for 3C-STREP-KKCKK-GFP11 (pFMP249) ...
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bioRxiv - Genetics 2024Quote: ... and cloning into the pJR1-41XL vector116 with the CloneEZ PCR Cloning Kit (GenScript). Plasmids were checked by PCR for correct insert size and also by Sanger sequencing (Eurofins Genomics ...
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bioRxiv - Biophysics 2021Quote: ... cyaA DNA was amplified from genomic DNA by PCR and cloned in pET-15b (GenScript) using AsuII and NcoI enzymes to generate plasmid pME14 ...
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bioRxiv - Microbiology 2023Quote: ... The resulting DNA was used as template for PCR amplification using Taq DNA polymerase (Genscript). DNA fragments resolved with electrophoresis of 1.2% Agarose gel purified using the Gel Extraction Kit (Qiagen ...
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bioRxiv - Genomics 2023Quote: The ACE2 coding sequence was synthesized by PCR amplification from the pUC57-ACE2 plasmid (GenScript). We included a NheI restriction site at the 5’ terminus and a PmeI restriction site at the 3’ terminus ...
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bioRxiv - Microbiology 2019Quote: ... cdeR and cseR were made by introducing PCR-generated amplicons or synthetic gene fragments (IDT or Genscript) into pEX18Gm-derived delivery plasmid (39) ...
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bioRxiv - Physiology 2022Quote: ... The purified PCR products and linearized vectors were assembled using a GenBuilder Plus kit (Genscript, L00744-10) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 N gene was PCR amplified from plasmids pUC57-SARS-CoV-2-N (GenScript) using forward primers with T7 promoter and reverse primers with poly(T)34 sequences ...
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bioRxiv - Microbiology 2023Quote: ... The SARS-CoV-2 N gene was PCR amplified from plasmids pUC57-SARS-CoV-2-N (GenScript) using forward primers with T7 promoter and reverse primers with poly(T)34 sequences ...
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bioRxiv - Bioengineering 2019Quote: ... The resulting PCR products were analyzed by agarose gel electrophoresis and DNA sequencing (GenScript Biotech Corp., Nanjing, China).
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bioRxiv - Cancer Biology 2022Quote: Human RET-GFP and RET712-1114-GFP were cloned by PCR using a cDNA clone (GenScript Biotech; NM_020975.6) as template ...
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bioRxiv - Plant Biology 2019Quote: ... PCR products were run in 2% agarose gel electrophoresis using a 100 bp DNA ladder (GenScript; CAT: M102O).
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR products were analysed by agarose gel electrophoresis and confirmed by Sanger sequencing (GenScript, Nanjing, China). The genome editing plasmids were cured following the method we previously developed (Zheng et al ...
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bioRxiv - Neuroscience 2019Quote: ... The GtACR1-EYFP fragment from the p7-GtCAR1 plasmid was swapped in using CloneEZ® PCR Cloning Kit (GenScript) for the myr::GFP fragment in pJFRC177 and the sequence was verified (GenScript) ...
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bioRxiv - Molecular Biology 2019Quote: ... MCM7 or CDC45 were amplified by PCR using Phusion polymerase (Thermo) from pcDNA3.1 plasmids encoding the cDNA of each protein (MCM plasmids from Genscript). The PCR products were then cloned into the pGBKT7-BD or pGADT7-AD plasmids ...
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bioRxiv - Microbiology 2020Quote: ... hnRNPUL1 (NM_007040) and PEG10 (NM_015068.3) were PCR amplified from ORF clones (hnRNPL, GenScript #OHu14072; hnRNPUL1, Origene #RC200576; PEG10, GenScript #OHu101111) and the products were cloned into the mammalian expression plasmid pCAGGS ...
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bioRxiv - Microbiology 2022Quote: All constructs were PCR amplified from a codon optimized gene block encoding the coding sequence of human DYRK1A (GenScript) using Q5 High-Fidelity DNA Polymerase with GC enhancer buffer (New England Biolabs) ...
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bioRxiv - Bioengineering 2022Quote: ... by means of overhang PCR and was cloned via traditional restriction/ligation into a pET28a(+) plasmid (ordered from GenScript) coding for the C-terminal flexible linker and small BiT (SB ...
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bioRxiv - Genetics 2021Quote: ... The predicted full-length lincRNA sequences were amplified by PCR and cloned into the pJR1-41XL vector (Moreno, Durán and Ribas, 2000) using the CloneEZ® PCR Cloning Kit (GenScript). Each plasmid was checked by PCR for correct insert size ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF was subcloned into the BamH1 site of pCS2+ (pCS2+-sobp) using the Clone EZ PCR cloning kit (GenScript). pCS2+-5’HA-sobp and pCS2+-sobp-3’HA were generated using the QuikChange lightning Site-directed mutagenesis kit (Agilent) ...
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bioRxiv - Biochemistry 2022Quote: The C-terminally His-tagged construct encoding human UGGT1 residues 43-1551 was PCR-amplified from the commercially sourced vector UGGT1-pUC57 (GenScript) with primers ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... and SUN2-N-2 (AA 1-226) fragments by PCR from pcDNA3.1+/C-(K)DY-SUN2 vector (OHu01874,GenScript # NM_001199579.1) and subsequent cloning into MP029-CRY2-mCherry lentiviral vector using the NheI/XbaI restriction sites ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... The construct consisting of nucleoplasmic and transmembrane domains of SUN2 and the luminal domain of SUN1 was created by amplifying SUN1 C-terminal fragment (AA 245-511) by PCR from pcDNA3.1+/C-(K)DY-SUN1 vector (OHu26731,GenScript # NM_001130965.3) using 5’-CTGTTCTAGAATGTTGGCTGGCCGTGG-3’forward and 5’-CTGTCTCGAGGCCCTGACTTGCACGTCCA-3’ reverse primers and subsequent cloning into MP029-CRY2-mCherry-SUN2-N-2 vector using the XbaI/XhoI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... was assembled from a PCR performed on a codon-optimized SARS-CoV-2 Omicron BA.1 sequence synthesized by Genscript. Fragments were assembled with a PCR fragment containing the fpl and mNG2(11 ...
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bioRxiv - Molecular Biology 2022Quote: DNA encoding aa 403-1390 of ATAD2 was PCR amplified from the full-length codon-optimized synthetic ATAD2 gene (Genscript), and subcloned into an in-house modified pFastBac1 vector with an N-terminal 3xFlag tag and Tev protease cleavage site using EcoRI and XhoI restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: The inducible L1 reporter plasmid used to generate HeLa tet-L1/GLucAI cells (pBH001) was generated through a series of successive PCR-based cloning steps performed by GenScript. pBH001 is comprised of a tetracycline-regulated bidirectional promoter for inducible expression of both firefly luciferase (a fragment cloned from the pTRE3G-BI-Luc control plasmid (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: PLIN1 and APEX2-V5 fragments were amplified by PCR from PLIN1 cDNA ORF clone in pcDNA3.1+/C-(K)-DYK vector (GenScript: OHu22113) and Twinkle-APEX2-V5 vector (Addgene ...
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bioRxiv - Biochemistry 2024Quote: Cloning of hPINK1 constructs for insect cell expression and test purifications The coding sequences for the PINK1 constructs were PCR amplified using clone OHu25380D (Genscript) as a template and cloned into the vector pFB-6HZB (SGC ...
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bioRxiv - Cell Biology 2024Quote: The human K6a sequence flanked by an amino-terminal HA tag and a carboxyl-terminal Flag tag (HA-hK6a-FLAG) was generated by PCR using pUC57-hK6a as a template which is codon-optimized for mammalian cell expression (Genscript). The sequence was inserted into lentiviral expression vector pCDH533-IRES-Neo (System Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... a GlySer-linker and the C-tag flanked by PmeI sites was amplified by PCR from a synthetic fragment provided by GenScript (USA), codon-optimized for expression in a low protease background C1 ...
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bioRxiv - Microbiology 2020Quote: ... The DNA fragment was then inserted into the KpnI and HindIII digested pLJ-Ssc70-Myc backbone using the CloneEZ™ PCR Cloning Kit (GenScript) to get pLJ-Hsp70-Myc plasmid ...
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bioRxiv - Microbiology 2023Quote: ... or with the mutated or truncated cdeA promoter regions were made by either PCR amplification or by DNA synthesis (Genscript, USA). The DNA products were made with incorporated kpnI or xbaI enzyme recognition sites and were digested with KpnI and XbaI and ligated to KpnI-XbaI-digested pMP220 [15] upstream of the lacZ reporter ...
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bioRxiv - Biochemistry 2022Quote: ... random mutagenesis library was generated by error-prone PCR (~8-10 mutations per kb) from the wildtype ecDHFR cDNA template (GenScript, Piscataway, NJ). The library was cloned into a pENTR1A plasmid backbone with a C-terminal YFP HA reporter tag using Gibson Assembly (HiFi DNA Assembly Cloning Kit ...