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Citations for GenScript :
1 - 50 of 561 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and subsequently cloned into pLenti-CMV-MCS-GFP-SV-puro using XbaI and BamHI to replace GFP or cloned directly into pLenti-CMV-MCS-GFP-SV-puro by Genscript using the same sites ...
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bioRxiv - Molecular Biology 2021Quote: ... pBABE-Puro-6xAU_HOTAIR and pBabe-Puro-14xAU_HOTAIR were synthesized and cloned by GenScript.
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bioRxiv - Cancer Biology 2022Quote: ... plenti CRISPR v2 virus against sgRNA targeting c-MYC (Genscript) and plenti CRISPR v2 virus (control ...
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bioRxiv - Neuroscience 2022Quote: ... or pLenti-TDP-43ΔNLS/2KQL-C-mGFP (Origene, mutations by GenScript). Cells were seeded onto coverslips in 24-well plates at a density of 25,000 cells/well and incubated for 24 h ...
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bioRxiv - Plant Biology 2023Quote: The constructs CMV::ACD6Col-0 and CMV::MHA1L were synthesized in pcDNA3.1 myc-6xHis or pcDNA3.1 mGFP5-6xHis (GenScript Biotech, NJ, USA). Two codon-optimized ACD6Col-0 sequences were obtained and synthesized from gBlock synthesis (GenScript ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene-specific sgRNA oligos were cloned into a pLenti CRISPR v2 plasmid (Genscript), which bicistronically expresses Cas9 nuclease ...
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bioRxiv - Cell Biology 2020Quote: To generate pLenti-mTomm70A-EGFP the open reading frame of mouse Tomm70A (GenScript OMu13526) was amplified via PCR and inserted into pcDNA-EGFP introducing a ten amino acid long linker between mTomm70A and EGFP (GGSGDPPVAT) ...
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bioRxiv - Cell Biology 2020Quote: ... To generate pLenti-mTimm50-mRFP the open reading frame of mouse Timm50 (GenScript OMu13400) was amplified via PCR and inserted into pcDNA-mRFP introducing a ten amino acid long linker between mTimm50 and mRFP (GGSGDPPVAT) ...
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bioRxiv - Immunology 2023Quote: Guides were synthetized and cloned into pLenti-sgLib-U6-IT-EF1a-mKate2-P2A-Neo by Genscript. Plasmids were packed into lentiviral particles using the Lenti-X cell line and Lenti-X Packaging Single Shots (VSV-G ...
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bioRxiv - Immunology 2023Quote: ... and cloned into the CMV/R vector (Barouch et al. 2005) by Genscript with a C-terminal hexahistidine affinity tag ...
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bioRxiv - Cell Biology 2022Quote: ... gRNAs were expressed from pSpCas9 BB-2A-Puro (PX459) v2.0 plasmids (Genscript). POLE1 plasmid (41 ...
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bioRxiv - Cancer Biology 2024Quote: ... and FLAG-MTH1-LAPTM5 cDNA were cloned into pGenLenti-puro vector (GenScript). Lentivirus particles were used to transduce HEK293T ...
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bioRxiv - Cancer Biology 2022Quote: ... They were synthesized and cloned into the pLentiGuide-Puro vector (GenScript, Piscataway NJ) to be packaged into lentivirus using the Lenti-X HEK293 cells ...
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bioRxiv - Bioengineering 2023Quote: ... 13441-16236) was synthesized and inserted into the pcDNA vector with a CMV promoter by GenScript. To express the Rdrp in RPMI 1650 cell and in mice ...
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Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium as a carrier in ratbioRxiv - Microbiology 2020Quote: The pcDNA3.1(+)-CMV-SARS-CoV-2-S-GFP (pSARS-CoV-2-S) plasmid was purchased from Genscript Co. ...
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bioRxiv - Microbiology 2024Quote: ... Stable DPP4 expressing HEK293T cells were generated by transducing HEK293T cells with hDPP4-puro (Genscript) lentivirus followed by selection with puromycin for 7 days ...
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bioRxiv - Immunology 2023Quote: Antibody heavy chain and light chain genes were synthesized and cloned into plasmids containing the CMV promoter (GenScript). Final heavy and light chain plasmids were amplified ...
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bioRxiv - Molecular Biology 2022Quote: ... SUMO4 knockout U2OSTrEx-FlpIn were generated using two different guide RNAs pSpCas9 (BB)-2A PURO (GenScript) plasmids that target the SUMO4 gene at nucleotides (relative to start codon ...
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bioRxiv - Molecular Biology 2022Quote: ... Full-length mouse IMPG1 variants were synthesized and inserted in a pcDNA3.1(+)-P2A-eGFP vector under a CMV promotor by Genscript. All IMPG1 proteins were expressed in HEK293 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... The spliced HOTAIR transcript (NR_003716.3) was synthesized and cloned into the pBABE-puro retroviral vector by GenScript. An antisense transcript of the firefly luciferase gene (AntiLuc ...
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bioRxiv - Cell Biology 2021Quote: ... which was synthesized to contain 7 modified TetO elements flanked by two minimal CMV promoter sequences based on pTet-T2 sequences (GenScript) (49) ...
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bioRxiv - Cell Biology 2022Quote: ... the mAp-PFN1 cassette was synthesized and inserted into pmApple-C1 behind the CMV promoter at the Ndel and BamHI restriction sites (Genscript). The amino acid linker and PFN1 was synthesized into the backbone of a pcDNA-Halo expression vector (provided by Gunther Hollopeter ...
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bioRxiv - Microbiology 2022Quote: ... a gene SIRT7 (C-terminally 3xFLAG tagged) under the control of a CMV promoter in pcDNA3.1 was commercially synthesized (GenScript, USA). For transfection experiments ...
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bioRxiv - Immunology 2020Quote: ... Genes for expression of HA fusions to nanoparticle trimeric components were codon optimized for expression in human cells and cloned into the CMV/R (VRC 8400) mammalian expression vector by Genscript. All HA fusions to the I53_dn5B trimer contained full-length HA ectodomains including native secretion signals ...
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bioRxiv - Molecular Biology 2021Quote: ... Variants of CMV-ubi-nsP4-RRV and CMV-ubi-nsP4-SINV containing mutations listed in supplementary figure S6 were constructed using synthetic DNA fragments (Genscript). Sequences of all plasmids were verified using Sanger sequencing and are available from the authors upon request.
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bioRxiv - Synthetic Biology 2022Quote: ... This gene was codon optimized for human cell expression and made in the CMV/R mammalian expression vector by Genscript. Transient transfection into HEK293F cells was carried out using PEI MAX ...
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bioRxiv - Biochemistry 2024Quote: ... fused to consecutive C-terminal HA (hemagglutinin)- and FLAG-tags and cloned into pCDNA-3.1 plasmid for expression in HEK293 cells by the CMV (cytomegalovirus) promoter (GenScript Biotech). Mutant variants were generated by site directed mutagenesis (GenScript Biotech).
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bioRxiv - Immunology 2023Quote: ... and were codon-optimized for human cell expression and made in the CMV/R vector (Barouch et al. 2005) by Genscript with a C-terminal hexahistidine affinity tag ...
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bioRxiv - Cell Biology 2020Quote: ... AAV production protocols were modified to include polyethylenimine co-transfection of an AAP-6 expression plasmid (ORF under the control of the CMV promoter, synthesized by GenScript Biotech), the variant 5 AAV cap plasmid ...
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bioRxiv - Physiology 2023Quote: ... expression plasmid consisting of a cytomegalovirus (CMV) promoter/enhancer and SV40 poly-A region flanked by AAV2 terminal repeats (pAAV2) by Genscript (Piscataway, USA) described previously38 ...
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bioRxiv - Cell Biology 2024Quote: ... we first obtained a pcDNA3.1-based plasmid containing both the human NCAPD2 cDNA under the control of the constitutive CMV promoter and the G418 resistance gene for selection in vertebrate cells (Genscript; pcDNA3.1-NCAPD2).
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bioRxiv - Developmental Biology 2020Quote: ... The LV-AEP vector was constructed by custom synthesizing the continuous open reading frame of ATOH7f-P2A-EGFP-T2A-Puro by GenScript, and replacing the EGFP gene in the LV-GFP vector ...
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bioRxiv - Cell Biology 2022Quote: The pSpCas9 BB-2A-Puro (PX459) plasmid backbone was used to create the following guide RNA (gRNA) plasmids (created by Genscript®): BNIP3 CRISPR gRNA plasmid (gRNA targeting sequence ...
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bioRxiv - Genetics 2020Quote: ... Notch 2 (1:100; LSBio) and Presenillin-1 (1:100; Genscript) overnight at 4°C ...
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bioRxiv - Pathology 2022Quote: ... Jagged-1 peptide (1 uM, Genscript), Y-27632 (10 uM ...
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bioRxiv - Genetics 2021Quote: ... rat anti-RAD-51 (1:500, (20)), guinea pig anti-SUN-1 S24pi (1:700, (72)), chicken anti-GFP (1:500, (A01694, Genscript)) ...
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bioRxiv - Cell Biology 2021Quote: ... Anti α-Tubulin (Genscript, 1:50-1:100); Anti α-Tubulin (proteintech ...
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CRISPR-based environmental biosurveillance assisted via artificial intelligence design of guide-RNAsbioRxiv - Molecular Biology 2024Quote: ... 1 µL of 1 µM crRNA (GenScript, custom) and 1 µL of 280 mM MgOAc (TwistDx) ...
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bioRxiv - Microbiology 2021Quote: ... anti-SpoVAD64 (1:10,000) and anti-His (1:4,000) (GenScript) antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... Dkk-1 (GenScript) was added to BM at final concentration of 100 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... FAT-1 (Genscript), and FAT-2 (Genscript ...
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bioRxiv - Microbiology 2024Quote: ... 1 (Genscript Biotech). Additionally ...
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bioRxiv - Genomics 2021Quote: ... Ada2b (rabbit polyclonal, 1:1000; GenScript anti-amino-acid 1-330); anti-Flag-horseradish peroxidase (mouse ...
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bioRxiv - Neuroscience 2021Quote: ... plko.1-CMV.Puro-tGFP-shFoxg1 or plko.1-CMV.Puro-tGFP-shLuciferase (Genscript) plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... 1:100 (A00487, Genscript). All secondary antibodies were purchased from Invitrogen and used at 1:700 and were Alexa Fluor-488 Donkey anti-rabbit (A21206) ...
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bioRxiv - Immunology 2022Quote: ... β-Actin (GenScript, 1:15,000), T-bet (clone 4B10 Santa Cruz ...
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bioRxiv - Immunology 2022Quote: ... β-actin (GenScript, 1:15,000), goat anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Biophysics 2024Quote: ... named EKAR-1 (GenScript). To create entry vectors ...
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bioRxiv - Biochemistry 2020Quote: ... 1-932 and EAV nsp9 1-693 was synthesized with codon optimization (Genscript) and cloned into pFastBac with an N-terminal MG addition and C-terminal TEV protease site and two Strep tags ...
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bioRxiv - Microbiology 2024Quote: ... Western blot membranes were probed with primary antibody (either 1:4000 rabbit anti-FLAG [Sigma Aldrich, St. Louis MO] or 1:4000 or 1:2000 mouse anti-StrepII [Genscript, Piscataway NJ]) for 1 hour at room temperature or overnight at 4°C and with secondary antibody (either 1:5000 goat anti-rabbit or anti-mouse respectively conjugated to horseradish peroxidase (HRP ...