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Citations for GenScript :
1 - 50 of 51 citations for pET28a DsRed2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... bearing a pET28a plasmid (Genscript) that encoded either APE1 or Pol β ...
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bioRxiv - Biochemistry 2024Quote: ... were inserted into pET28a(+) plasmids (Genscript) and transformed into BL21 (DE3 ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into pET28a(+)-TEV vector (GenScript). Protein was expressed in E ...
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bioRxiv - Cell Biology 2022Quote: ... coli expression vector pET28a (GenScript, Piscataway, USA). The plasmid vector was transformed into E ...
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Adaptation of the Romanomermis culicivorax CCA-adding enzyme to miniaturized armless tRNA substratesbioRxiv - Molecular Biology 2020Quote: ... coli and synthesized in pET28a by GenScript® (Piscataway ...
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bioRxiv - Cell Biology 2022Quote: ... coli using the pET28a vector (GenScript, USA). BDNF was diluted either in saline for in vivo experiments or in DMEM for in vitro experiments ...
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bioRxiv - Biophysics 2021Quote: ... and subcloned into pET28a by GenScript (Piscataway, USA). Expression and purification of these constructs were previously described by the international Covid19-NMR consortium (https://covid19-nmr.de/ ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli and sub-cloned into pET28a(+) vectors (Genscript). Each ferredoxin was expressed in BL21(DE3 ...
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bioRxiv - Biochemistry 2022Quote: ... was synthesized and cloned into the pET28a vector between Nco I and Xho I restriction sites to create the construct pET28a-lsMSP2N2 by GenScript (Piscataway, NJ). The expression constructs for the mutant spike proteins were generated by standard PCR methods and verified by DNA sequencing of the entire coding regions.
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bioRxiv - Bioengineering 2021Quote: ... Genes were synthesized and cloned into pET28a(+) vector by Genscript, and resuspended to 0.2μg/μL with ddH2O.
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bioRxiv - Biochemistry 2024Quote: CeEngase (eng-1) gene was cloned into pET28a vector (Genscript). The constructed pET28a-eng-1 plasmid was transformed into E ...
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bioRxiv - Biochemistry 2021Quote: ... SMT (SUMO-like tag) fusion protein in a pET28a vector (Genscript). Quick Change mutagenesis was performed to generate the W611A mutant of hP13 ZnF5-WWE1-WWE2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... was ordered codon optimized and sub-cloned into pET28a(+) vectors (Genscript). TsrM was co-expressed together with the Btu operon in BL21(DE3 ...
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bioRxiv - Microbiology 2024Quote: ... into pET28a-His6 using NdeI and BamHI by GenScript (NJ, USA). pET28a-coaXDM_his was synthesized similarly by Twist Bioscience (CA ...
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bioRxiv - Biochemistry 2024Quote: The human SRSF3-pET28a clone was purchased from Genscript (Piscataway, NJ). Different SRSF3 variants were subcloned in pET28a and expressed as TEV protease-cleavable N-terminal 6xHis-tag fusions in E.coli Rosetta2(DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... human CSPβ fused to His6 in pET28a was from GenScript (NJ, USA), full-length human NSF fused to His6 in pET28 was a kind gift from Dr ...
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bioRxiv - Cell Biology 2022Quote: ... Human CSPβ fused to His6 in pET28a was from GenScript (NJ, USA). The rabbit polyclonal anti-CSP antibody (affinity-purified with the immunogen directed towards the amino acids 182-198 of rat CSP) ...
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bioRxiv - Microbiology 2022Quote: ... and cloned into the pET28a expression plasmid as custom orders by GenScript Biotech.
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bioRxiv - Biochemistry 2020Quote: ... all PDZ domain coding sequences were inserted into the pET28a+ vector (GenScript) with an N-terminal His-tag and cleavable PreScission site ...
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bioRxiv - Bioengineering 2022Quote: We obtained the genes encoding the designed proteins in pET28a vectors from GenScript (Genscript.com). We confirmed the sequences of all the constructs by DNA sequencing (Eton bioscience ...
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bioRxiv - Biochemistry 2021Quote: ... codon optimized human DHFR was produced as a 6His-SUMO1 fusion from pET28a (Genscript).
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bioRxiv - Biochemistry 2024Quote: ... All proteins were expressed as His6-SUMO fusion proteins using the pET28a(+) plasmid (Genscript). Expression and purification protocols were similar to those used previously for other SLiM-binding domains (66–68) ...
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bioRxiv - Biochemistry 2023Quote: ... The Galectin1 and Endo F3 gene were synthesized and cloned to pET28a plasmid by GenScript Corporation ...
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bioRxiv - Biochemistry 2024Quote: The gene for FGF1 was codon optimised for E.coli expression and ordered in pET28a (Genscript).
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bioRxiv - Biochemistry 2024Quote: The mTurq2-DNPKTGDE and GG-SYFP2 sequences were also inserted into the pET28a(+) plasmid (Genscript) and included N-terminal His6 and TEV cleavage site purification tags ...
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bioRxiv - Cancer Biology 2021Quote: ... FnEDB E42Q D68N E64Q) were synthesized and cloned into pET28a+ expression vectors (GenScript Biotech BV, Netherlands). The resulting plasmids were transformed into the E ...
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bioRxiv - Biochemistry 2021Quote: ... The wild-type protein and the mutant K96A cloned in pET28a vector were ordered from GenScript. The N-terminally truncated constructs were cloned by amplifying the sequence from the original vector and subcloning into BsaI-cleaved plasmid pNIC28_Bsa4 by SLiCE cloning (83) ...
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bioRxiv - Biochemistry 2022Quote: Codon optimized human wild-type full-length (FL) APE1 in a pet28a vector was purchased from GenScript. The E96A/D210N ...
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bioRxiv - Biochemistry 2022Quote: ... Codon-optimized versions of the following protein sequences were synthesized and subcloned into the pET28a vector (GenScript): Different syntaxin-1a (1– 262 ...
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bioRxiv - Genomics 2022Quote: ... Codon-optimized versions of the following SNARE protein sequences were synthesized and subcloned into the pET28a vector (GenScript): Acanthamoeba polyphaga mimivirus ...
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bioRxiv - Synthetic Biology 2021Quote: Coding sequences of all tested IspG proteins were ordered codon optimized and cloned in a pET28a(+) plasmid by Genscript. A Tobacco etch virus (TEV ...
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bioRxiv - Bioengineering 2022Quote: ... by means of overhang PCR and was cloned via traditional restriction/ligation into a pET28a(+) plasmid (ordered from GenScript) coding for the C-terminal flexible linker and small BiT (SB ...
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bioRxiv - Biochemistry 2024Quote: Codon-optimized RdRp and VPg genes of HuNoV GII.4 Sydney strain (GenBank: AFV08794.1) were synthesized and cloned in the pET28a vector for bacterial expression by Genscript U.S.A ...
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bioRxiv - Biophysics 2024Quote: ... RdRp and MTase domains (New Guinea C strain, GenBank no AF038403.1) were codon-optimized and cloned into the pET28a(+)-TEV vector (GenScript) with a hexahistidine tag ...
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bioRxiv - Molecular Biology 2020Quote: Codon optimized Gcn5 (S. pombe) with 1 × FLAG was cloned into pET28a in frame with N terminal 6 × HIS tag by GenScript to generate JP-2587.
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bioRxiv - Microbiology 2020Quote: The I53-50B.4PT1 was codon-optimized and synthesized and then cloned into a modified pET28a* vector with a C-terminal hexahistidine tag by Genscript.
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bioRxiv - Biochemistry 2022Quote: ... synthesized and subcloned into the pET28a(+) vector with restriction cleavage by NdeI/XhoI to carry both N- and C-terminal His6 tags (Genscript). Primers carrying a G155A or G225V mutation were designed for site-directed mutagenesis using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Biochemistry 2022Quote: cDNA encoding His6-TEV-pro-conA sequence was synthesized and sub-cloned into pET28a(+) in framed with the N-terminal His-tag (Genscript), followed by transformation into Rosetta PLysS competent cells (Novagen) ...
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bioRxiv - Biophysics 2024Quote: ... A pET28a(+) plasmid containing an open reading frame for human hnRNPA1 with an N-terminal 6xHis tag was synthesized by GenScript. The 6xHis-hnRNPA1 was expressed in E ...
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bioRxiv - Biochemistry 2023Quote: ... wild-type APE1 was expressed in the absence of any tags from a pet28a codon optimized clone purchased from GenScript. The plasmid was transformed into One Shot BL21(DE3)plysS E ...
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bioRxiv - Biophysics 2023Quote: The plasmids for the expression of codon optimized versions of G4Ps and variants with N-terminal FLAG-His tags in the pET28a backbone were synthesized by GenScript.
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bioRxiv - Biochemistry 2023Quote: The open reading sequences encoding TcdB1-GTD to TcdB8-GTD were synthesized with a C-terminal His6 tag and cloned into pET28a(+) by Genscript. Sequence length and strain information is shown in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: Human Mb constructs (WT Mb, Mb K46M) were cloned into pET28a(+) vectors with bacterially optimized codons and purchased from GenScript Biotech Corp ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids containing the gene of interest fused to an N-terminal His6 tag in the pet28A vector were obtained from Genscript and transformed into BL21-DE3 competent cells (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... Codon-optimized versions of the following SNARE domain sequences from Mus musculus were synthesized and subcloned into the pET28a vector (GenScript): Syntaxin 5 (aa 199-280 ...
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bioRxiv - Biochemistry 2024Quote: ... a plasmid containing the target gene in the pet28A vector fused to an N-terminal His6 tag (twenty additional residues added) was acquired from Genscript and transformed into BL21-DE3 competent cells (New England Biolabs) ...
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bioRxiv - Biochemistry 2021Quote: ... The cDNAs encoding mCer-mCit sensor or CRONOS were synthesized and subcloned into pET28a vector containing His6 tag (Genscript, NJ, USA).
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bioRxiv - Biochemistry 2022Quote: The coding sequence of MtDPP was cloned into plasmid pET28a(+)in frame with an N-terminal 6×His tag (GenScript™). BL21 (DE3 ...
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bioRxiv - Plant Biology 2022Quote: ... Sequences starting after the residue corresponding to butelase-1-L26 or after the signal peptide predicted using SignalP5.0 were cloned into the pET28a(+) vector at Ndel/Xhol restriction sites to generate a His6-fusion protein construct (Genscript, USA). Point mutations were generated using a Q5 mutagenesis kit (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... coding sequence was synthesised and cloned into a pET28a plasmid with N-terminal His6-tev purification tag (supplied by Genscript Ltd).