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Citations for GenScript :
1 - 50 of 823 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Codon-optimized cDNAs containing the effector protein RfxCas13d were synthesized (Genscript) and assembled into modified pET28 vectors by NEBuilder HiFi DNA assembly (E2621 ...
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bioRxiv - Biochemistry 2023Quote: ... thirteen 601 repeats were synthesized (GenScript) in which naturally occurring AvaI and AluI sites in the canonical 601 sequenced were replaced with unique restriction sites ...
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bioRxiv - Immunology 2021Quote: ... biotinylated detection antibody (GenScript, Cat# 5E10G8-Biotin) was added at 1 µg/mL final concentration in blocking buffer and plate was incubated at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The 24 PP7 repeats were synthesized (GenScript) along with Hba-a1 coding sequences between two KflI sites at the 5’ end of the gene and subsequently exchanged by standard restriction enzyme digestion and re-ligation with sequence in the above p15 plasmid to obtain the final p15A-loxP-Hba-a1-PP7-lox511 construct to be used as the RMCE donor vector.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides containing an N-terminal biotin tag were purchased from GenScript and dissolved according to manufacturer’s recommendation ...
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bioRxiv - Developmental Biology 2021Quote: ... or the truncated version of Npnt containing only the N-terminal EGF-like repeats domain (Npnt-EGF) were commercially synthesized (Genscript) and cloned into the modified RCAS vector ...
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bioRxiv - Neuroscience 2022Quote: ... we obtained an expression plasmid containing a C-terminal 3Xflag tagged BioID2 sequence with a 198bp (13X “GGGGS” repeat) linker sequence upstream of BioID2 (Genscript). For lentiviral expression ...
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bioRxiv - Neuroscience 2022Quote: ... we obtained an expression construct containing a 198bp (13x “GGGGS” repeat) linker sequence upstream of a C-terminal 3xFLAG-tagged BioID2 sequence with BioID2 (Genscript). For lentiviral expression ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids containing the N171 N-terminal fragment sequence of human HTT bearing either 85 CAG repeats (HTT85Q, pathological HTT fragment) or 10 CAG repeats (HTT10Q, control HTT fragment) were manufactured by GenScript and subsequently cloned into a transgene cassette flanked by viral inverted terminal repeats (ITRs) ...
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bioRxiv - Biophysics 2024Quote: 5X repeat Nck SH3 domain 2 (polySH3) and 5X repeat Abl PRM (polyPRM) codon-optimized genes were purchased from GenScript and cloned into a pMal plasmid to generate fusion proteins with an N-terminal Maltose Binding Protein (MBP ...
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bioRxiv - Immunology 2019Quote: ... the cells were stained with Biotin-Protein L (Genscript) followed by fluorescently-conjugated streptavidin.
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bioRxiv - Microbiology 2020Quote: ... 21 crRNA-encoding spacers separated by repeats were synthesized by GenScript and provided on a pUC57 vector (pMME1170) ...
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bioRxiv - Biophysics 2024Quote: ... a plasmid encoding 26X repeats of the miR-34a transcript (Genscript) was transcribed via T7 RNA polymerase (T7 RNAP ...
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bioRxiv - Cell Biology 2024Quote: ... T4E in every other repeat) were ordered as synthetic genes (Genscript), amplified ...
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bioRxiv - Microbiology 2021Quote: Mutant csp repeat region oligonucleotides were commercially synthesized by Genscript (Piscataway, NJ) and provided in the pUC57 plasmid ...
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bioRxiv - Biophysics 2022Quote: The peptide library spanning the tau repeat domain residues was synthesized from GenScript, ensuring each peptide was of sufficient purity (>90% ...
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bioRxiv - Microbiology 2022Quote: ... which were coated in anti-HIS antibody [Biotin] (GenScript A00613, mouse IgG1k clone 6G2A9) at 2.5 μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... Biotin-labeled mouse monoclonal antibody against the Strep-tagII (“NWSHPQFEK”) was purchased from Genscript (GenScript Cat# A01737 ...
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bioRxiv - Biophysics 2020Quote: The peptide with 25 repeats of proline-arginine (PR25) was purchased from GenScript (Hong Kong). PR25 was dissolved in water and then mixed with polyU at final concentration 100 μM and 1 μg/μl respectively to induce the LLPS.
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bioRxiv - Cancer Biology 2022Quote: ... or 25 GGAA-repeats upstream of the recently described minimal activity promoter YB-TATA (Genscript) into the newly designed MCS by PacI-EcoRV restriction digest23.
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bioRxiv - Plant Biology 2020Quote: ... we obtained DNA fragments matching the coding sequence of the effector domains by gene synthesis (GenScript, Pistacaway, USA) including PacI and NotI restriction sites on 5’ and 3’ ends ...
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bioRxiv - Plant Biology 2022Quote: ... the aphid (Myzus persicae) effector Mp10 was used as a positive control 72 Flg22 (cat. no. RP19986, GenScript) was used as an inducer of ROS production ...
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bioRxiv - Cell Biology 2020Quote: ... a PshAI/XhoI cDNA fragment encoding normal CEL with 16 VNTR repeats was synthesized by Genscript and used to replace the corresponding segment in pcDNA3/CEL-WT 14R ...
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bioRxiv - Immunology 2024Quote: ... The biotin-K417N peptide (VRQIAPGQTGNIADYNYKLP ; GenScript) was mixed with the streptavidin-AF647 in a 4:1 ratio at room temperature for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... The nucleotide sequences of nine tandem repeats were synthesized and cloned into pET14b vector (GenScript, Piscataway, NJ), which was used to express protein with N’ 6xHistidine-tagging (6xHis ...
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bioRxiv - Immunology 2020Quote: ... Biotin-Protein L was purchased from GenScript. BsiWI was purchased from New England Biolabs ...
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bioRxiv - Biophysics 2022Quote: ... Tandem repeat BtuC-BtuC-BtuD2 constructs (EE, EQ and QQ)16 were made by gene synthesis (Genscript, Piscataway, NJ), adding a (Gly4Ser)5 linker at the C terminus of BtuD ...
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bioRxiv - Immunology 2023Quote: ... supernatants containing monoclonal antibodies were purified using Protein A magnetic beads (Genscript), and the purified samples were verified by SDS-PAGE.
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bioRxiv - Synthetic Biology 2019Quote: A gene encoding a smaller version of MpIBP (Genbank accession: ABL74378.1 and ABL74377.1) with only 15 central repeats was synthesized and purchased from GenScript (https://www.genscript.com). The gene was codon-optimized for expression in E ...
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bioRxiv - Synthetic Biology 2020Quote: ... A synthetic promoter (PLexA) consisting of eight repeats of the LexA operator and a minimal GAL1 promoter was also synthesised (GenScript). LexA-DAD2 ...
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bioRxiv - Immunology 2023Quote: ... 2 µg/mL biotin conjugated Env peptides (GenScript, customized) were added to plates and incubated for 1 hour at RT ...
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bioRxiv - Bioengineering 2019Quote: ... a DNA block composed of the leader sequence of the chromosomal CRISPR2 as a promoter and two CRISPR repeats spaced by two BsaI restriction sequences in opposite orientation was synthesized from GenScript (Nanjing, China), and used as a template for PCR amplification with the primer pair of L2R-XbaI-F/L2R-EcoRI-R (Table 3) ...
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bioRxiv - Physiology 2023Quote: ... expression plasmid consisting of a cytomegalovirus (CMV) promoter/enhancer and SV40 poly-A region flanked by AAV2 terminal repeats (pAAV2) by Genscript (Piscataway, USA) described previously38 ...
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bioRxiv - Genetics 2023Quote: ... Plasmids pML107 11 or PAEF5 12 carrying gRNA were co-transformed with 200bp DNA repair recombinant donor sequences carrying telomeric repeats seeds (Genscript Biotech Netherlands) (Figure Supp 1A).
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bioRxiv - Immunology 2023Quote: ... supernatants containing the monoclonal antibodies were purified using protein A magnetic beads (Genscript, L00695). The purified samples were determined by SDS-PAGE.
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bioRxiv - Neuroscience 2023Quote: ... were reacted with equimolar cell-degradable peptide containing sequence KCGPQGIWGQCK (GenScript), 3.0 mM CRGDS basement membrane-mimicking peptide (GenScript) ...
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bioRxiv - Immunology 2021Quote: ... Antibody VH or VL sequences were cloned into plasmids containing an IgG1 or relevant light chain backbone (Genscript) and used to transfect Expi293 cells (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Antibody heavy chain and light chain genes were synthesized and cloned into plasmids containing the CMV promoter (GenScript). Final heavy and light chain plasmids were amplified ...
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bioRxiv - Immunology 2023Quote: ... Antibody VH or VL sequences were cloned into plasmids containing an IgG1 or relevant light chain backbone (GenScript) and transfected into Expi293 cells (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with mouse anti-HA antibody (Genscript) diluted 1:500 in PBS supplemented with 0.5% goat serum and 0.01% Tween-20 (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: K562 cells expressing luciferase were transfected with a plasmid containing full-length EGFR (#OHu25437D, GenScript) using lipofectamine 2000 according to the manufacturer’s protocol (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... Phospho-peptides conjugated to biotin for ELISA assay were synthesized by GenScript (Hong Kong). Antibodies against the IR β-subunit (sc-57342 ...
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bioRxiv - Immunology 2022Quote: ... Matched pairs of antibody VH and Vλ/Vκ sequences were commercially cloned into plasmids containing an IgG1 or relevant light chain backbone and expressed as recombinant antibody (Genscript). mAbs were also expressed in-house by transient transfection of Expi293 cells (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The expression of each anti-HIV-1 CAR was detected by protein L-biotin (GenScript) and Alexa488-conjugated anti-human Fc antibody or APC-conjugated anti-human Fc antibody as described elsewhere [78] ...
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bioRxiv - Biochemistry 2023Quote: N-terminally biotinylated synthetic MUC1 peptide with the sequence biotin-GGS-APDTRPAPG was ordered from Genscript. This was dissolved in PBS and printed on a planar streptavidin-coated SPR chip (P-Strep ...
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bioRxiv - Microbiology 2021Quote: ... pH 7.5) containing 3 % (w/v) skim milk powder and incubated with a rabbit anti-SLPMh 133-147 primary antibody (GenScript, Leiden, Netherlands), diluted 1:200 in TBS (10 mM TRIS ...
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bioRxiv - Biochemistry 2022Quote: ... 0.2mg/ml BSA with either fixed or varying concentrations of either the peptide substrate (Biotin-GGGENWYNTLKRKK-NH2) (Genscript) or ATP spiked with 0.8μCi [γ 32P]-ATP (Perkin Elmer) ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were commercially synthesized and biotin-labeled on the N-terminus using Fmoc chemistry (GenScript, Piscataway, NJ, USA). Sequences of these peptides are shown in Table 1.
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bioRxiv - Cell Biology 2023Quote: Samples containing 1.2X Sample Buffer (Genscript) were separated via SDS-PAGE using 4- 12% Bis-Tris (Genscript ...
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bioRxiv - Synthetic Biology 2023Quote: ... This was then back diluted 1:10 into DMEM-FBS media used for growing LLC-sppIP cells or fresh media containing synthetic sppIP peptide (Genscript). Media from LLC-sppIP cells was collected after 24 h of growth in 96 well plates.