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Citations for GenScript :
301 - 350 of 922 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: A human Kif15 motor domain and neck linker construct (Kif15_MD residues 1-375) in a pET21a vector with a C-terminal 6 x His-tag was generated by chemical synthesis (GenScript, Piscataway, NJ). Six of the eight cysteine residues (C5S ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids for codon-optimized pET-28a- His6-nsp7-8 and pET-28a-His6-nsp7-11 (with an HRV 3C protease cleavage site between the 6x- His tag and the coding sequence) were obtained from GenScript (Piscataway, NJ). Primers used for cloning and mutagenesis ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid containing the full-length Arabidopsis thaliana Atm3 (AtAtm3) gene with a C-terminal 6x-His tag was purchased from Genscript (Genscript, NJ). Mutagenesis reactions generating the N-terminal 60 ...
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bioRxiv - Biophysics 2021Quote: ... coli optimized codons for the C0-C2 portion of human cMyBP-C with N-terminal 6x His tag and TEV protease cleavage site were obtained from GenScript (Piscataway, NJ). For TR-FRET binding assays ...
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bioRxiv - Evolutionary Biology 2021Quote: ... His-PFOR over-expression in the 6-L cultures was assessed by SDS PAGE analysis followed by immunoblotting with a His-tag specific antibody (GenScript; Fig S7). A specific band could be detected in the over-expression mutant ...
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bioRxiv - Biochemistry 2021Quote: ... The cDNAs were then cloned into pET-15b such that the expressed proteins would contain an N-terminal His-Tag (GenScript, Piscataway, NJ). Transformation of competent DH5α E ...
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bioRxiv - Microbiology 2022Quote: ... codon optimized genes encoding each PcaLOOL were obtained as subcloned in pPICZαA plasmids with a C-terminal 6 x His tag (GenScript, Piscataway, NJ, USA). P ...
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bioRxiv - Immunology 2020Quote: ... The S1-N-terminal domain (S1-NTD, amino acids 16-318) was custom synthesized by GenScript. Each protein was expressed with an N-terminal His6-Tag to facilitate purification ...
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bioRxiv - Biophysics 2022Quote: ... for 2 minutes followed by 20 μM biotinylated FLAG-tag antibody (A01429, GenScript) for 30 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... The coding sequences of both domains were optimized for codon usage of the host (E. coli BL21) using OptimumGeneTM (GenScript, USA) and a His-Tag was added at the carboxyl end for further purification ...
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bioRxiv - Microbiology 2022Quote: ... Heterologous expression was detected by electroblotting SDS-PAGE Tris-glycine gels onto nitrocellulose membranes and immunostaining with primary rabbit anti-His-tag antibody and secondary goat anti-rabbit IgG phosphatase alkaline conjugated antibody (GenScript, Piscataway, NJ, USA) (Suppl ...
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bioRxiv - Bioengineering 2024Quote: Genes encoding the designed protein sequence were synthesized and cloned into pET-24a(+) E.coli plasmid expression vectors (Genscript, C-terminal 6X His tag). Plasmids were then transformed into chemically competent BL21(DE3 ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: Recombinant SARS-CoV-2 wild-type S protein RBD-HRP fusion protein (RBD-HRP protein, cat. no. Z03594) and hACE2 protein (cat. no. Z03516) were purchased from GenScript Korea Ltd ...
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bioRxiv - Microbiology 2020Quote: ... interaction between CD147 and SP (RBD) (GenScript, China) was detected using Kinetic Analysis/Concentration Series/Direct Binding mode ...
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bioRxiv - Biochemistry 2020Quote: ... of SARS-CoV-2 (D614) and the ectodomain of human angiotensin converting enzyme 2 (ACE2; residue 1-615) were synthesized by GenScript (Piscataway, NJ). The S gene was fused with a C-terminal twin Strep tag [(GGGGS)2WSHPQFEK(GGGGS)2WSHPQFEK)] and cloned into a mammalian cell expression vector pCMV-IRES-puro (Codex BioSolutions ...
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bioRxiv - Immunology 2023Quote: ... expressing spike or STX-N_pLenti (Genscript) expressing nucleocapsid using Lipofectamine 3000 according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... expressing spike or STX-H3_pGenlenti (Genscript) at a ratio of 5:5:1 using Lipofectamine 3000 according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... by subcutaneous injection with 50μL S1 (Genscript) (50μg/rat ...
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bioRxiv - Microbiology 2024Quote: ... Table S1) and cloned into pUC57 (GenScript). A previously published gfp sequence (Takacs et al. ...
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bioRxiv - Immunology 2023Quote: A commercially available kit to quantify the ability of the three pAbs to neutralize the binding of RBD to ACE-2 was obtained from GenScript, Piscataway NJ (kit #L00847) ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using polyethylenimine (PEI ...
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bioRxiv - Microbiology 2019Quote: ... and 1 µg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Immunology 2021Quote: Antibodies inhibiting virus binding to host cell was measured using a commercial RBD-human angiotensin-converting enzyme 2 (hACE2) binding inhibition assay called cPASS™ (GenScript). As per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: RBD protein expressed with AviTag was purchased from GenScript. Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity) ...
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bioRxiv - Immunology 2021Quote: ... The B.1.429 RBD gene was synthesized by GenScript into pCMVR with the same boundaries and construct details with a mutation at L452R ...
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bioRxiv - Bioengineering 2021Quote: RBD protein expressed with AviTag was purchased from GenScript. Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity) ...
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bioRxiv - Biochemistry 2020Quote: ... coli (GenScript) and included a Gly-Ser linker (encoded by an in-frame BamHI site ...
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bioRxiv - Biochemistry 2022Quote: ... coli (Genscript) and subsequently cloned into pRSF-Duet1 (Novagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... coli (GenScript) and placed in the pSY5 vector3,26 ...
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bioRxiv - Biophysics 2022Quote: ... coli (GenScript). In each case ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (GenScript) and cloned into pET28-Sumo3 vector (EMBL ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Genscript) and subcloned using ligation-independent cloning into pMCSG7 (61) ...
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bioRxiv - Plant Biology 2024Quote: ... coli (Genscript) and cloned into the pET-22b expression vector (Novagen ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μg repair plasmid (Genscript, Note S1), followed by electroporation using program B024 of Nucleofector™ 2b Device (Lonza #AAB-1001) ...
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bioRxiv - Biochemistry 2021Quote: pcDNA3.1-spike and pcDNA-3.1-spikeΔfurin (Genscript) were fused with V5 epitope at the C-terminus to make pcDNA3.1-spike-V5 and pcDNA-3.1-spikeΔfurin-V5.
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bioRxiv - Microbiology 2022Quote: ... Mutant spike plasmids were produced by Genscript. Pseudoviruses were mixed with 8 serial 4-fold dilutions of sera or antibodies in triplicate and then added to monolayers of 293T-hACE2 cells in triplicate ...
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bioRxiv - Biochemistry 2023Quote: ... The gene encoding for the GAF2 domain from All1280 of Nostoc PCC 7120 (NCBI protein ID BAB73237.1, UniProtKB Q8YXD3, amino acids 562-727) was ordered from Genscript (codon optimized for E. coli) in the pUC18 cloning vector ...
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bioRxiv - Microbiology 2020Quote: ... the cDNA for PbChiB2 (Fig. S1) and PbChiB4 (Fig. S1) without the signal peptide was synthesized and cloned into plasmid pET-14b (GenScript, USA). Plasmids were used to transform E ...
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bioRxiv - Genetics 2021Quote: ... we stained the yeast with recombinant CoV2 RBD-His6 (Genscript) at diminishing concentrations through the rounds (5nM ...
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bioRxiv - Immunology 2024Quote: ... 293F cells were transfected with plasmids containing Spike protein (ATUM plasmid pD2528-CMV with insert QHD43416.1) and Spike-2P protein (site-directed mutagenesis to generate the 2P mutation on the plasmid containing Spike protein, GenScript) by 293fectinTM transfection reagent (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Peptides (Table S1) were synthesised at >90% purity (GenScript), stored lyophilised at −80°C and then reconstituted in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Treatments with either Spike (100nM, Cat#Z03485, Genscript) or the NRP-1 inhibitor EG-00229 (30µM ...
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bioRxiv - Biochemistry 2022Quote: ... from various snake species were selected from NCBI databases and cloned into a mammalian expression vector containing a C-terminal Avi™ tag followed by a WELQut™ cleavage tag and a rabbit Fc tag by Genscript. Plasmid DNA was prepped and co-transfected with a plasmid expressing the BirA enzyme for in vivo biotinylation into Expi293 cells using FectoPRO® (Polyplus Transfection) ...
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bioRxiv - Biophysics 2019Quote: ... coli expression (Genscript) and further subcloned in pET28a vector using NDE1 and Xho1 sites ...
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bioRxiv - Biochemistry 2020Quote: ... coli (Genscript, NJ) were incorporated into the multi-cloning site in the pET21a(+ ...
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bioRxiv - Biochemistry 2020Quote: ... coli by GenScript and subcloned into a pET30 vector (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... coli by Genscript Corporation ...
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bioRxiv - Microbiology 2020Quote: ... coli lysate (Genscript) at final concentration of 10 mg/mL to block anti-E ...