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Citations for GenScript :
401 - 450 of 656 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The tag was removed by histidine tagged Bovine Enterokinase (GenScript Biotech Corporation) before using the proteins for DNA binding assays.
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1:200 rabbit anti-S tag (Genscript A00625) overnight and 1:1000 mouse anti GFP (Thermo Fisher A-11120 ...
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bioRxiv - Bioengineering 2021Quote: Monoclonal antibodies against GAA and GILT-tag sequence were generated by Genscript. Mouse plasma samples were screened for the presence of GAA protein using JessTM ProteinSimple and following manufacturer’s protocol (ProteinSimple ...
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bioRxiv - Microbiology 2022Quote: ... and pangolin) with a C-terminal V5 tag were synthesized by GenScript as described previously 42 ...
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bioRxiv - Synthetic Biology 2020Quote: DNA sequences encoding proteins with 6xHis tags were codon-optimized by Genscript and cloned into pET28b+ or pET29b+ vector under the control of a T7 promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... USP7 was expressed with an N-terminal myc-tag from pcDNA3.1 (Genscript). All mutations were generated by Genscript ...
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bioRxiv - Biochemistry 2021Quote: ... a mouse monoclonal DKY-Tag antibody diluted 1:1,000 (GenScript, Cat# A00187) and a mouse monoclonal anti-β-Actin antibody diluted 1:1,500 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... which contains an N-terminal pelB signal sequence and hexahistidine tag (Genscript). The construct was transformed into chemically competent C41 (DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... gene blocks containing A5 fused with alternative purification tags (Genscript; Table 1) were inserted via standard cloning techniques and digested with NcoI and NotI (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... using an anti-Histidine-tag primary antibody (Genscript A00186; 1:3000 dilution) and an IR800 conjugated secondary antibody (Li-Cor Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 B.1.617 and B.1.1.7 variant Spikes were codon-optimized and synthesized by GenScript Inc (Nanjing ...
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bioRxiv - Microbiology 2021Quote: ... GenBank accession no. NC_004718.3) and WIV1-CoV spike (GenBank accession no. KC881007.1) expression plasmids were synthesized by Genscript and codon-optimized for human expression ...
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bioRxiv - Microbiology 2021Quote: ... N501Y.V1 (Variant 1) mutant Spike proteins of SARS-CoV-2 were codon-optimized and synthesized by GenScript Inc (Nanjing ...
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bioRxiv - Immunology 2020Quote: ... or 1-10 μg of Spike RBD protein (Sino Biological, Cat: 40592-V08H or GenScript, Cat: Z03483). “Mock” groups received PBS alone ...
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bioRxiv - Immunology 2022Quote: ... The Delta B1.617.2 variant clone was generated by gene synthesis with a codon optimized Spike ORF (GenScript). The final constructs encode the Spike ectodomains ...
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bioRxiv - Immunology 2023Quote: Lentiviral vectors for expression of SARS-CoV-2 spike (Delta variant B.1.617.2, NCBI accession # OX014251.1) were designed and synthesized from Genscript together with the two packaging plasmids (pMD2.G and psPAX2) ...
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bioRxiv - Immunology 2021Quote: ... An E gene DNA standard (pUC57-2019-nCoV-PC:E, GenScript, Piscataway, NJ) was also run at the same time for conversion of Ct value to genomic copies ...
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bioRxiv - Immunology 2021Quote: ... the E subgenomic mRNA sequence was inserted into a pcDNA3.1 vector (Genscript) and transcribed using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA probes (Table S1) were synthesized and labeled with 6-FAM at the 5’ end by GenScript (Nanjing, China). For the RNA electrophoresis mobility shift assays (REMSAs) ...
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bioRxiv - Microbiology 2020Quote: ... was used to generate a cDNA that was codon-optimized (see Fig. S1) and synthesized by GenScript (Piscataway, NJ) for expression in E ...
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bioRxiv - Molecular Biology 2022Quote: IgGFc: Human IgG Fc Sequence (Supplementary Material Figure S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
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bioRxiv - Microbiology 2022Quote: ... The primary antibody for Western blot is Mouse-anti-His mAb (GenScript, Cat.No.A00186). The concentration was determined by BCA protein assay with BSA as a standard ...
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bioRxiv - Biochemistry 2024Quote: ... 0.01-0.08 picomoles of FLAG-Fzd4 were probed by THE anti-His (GenScript) and M1 anti-FLAG antibodies respectively ...
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bioRxiv - Biochemistry 2019Quote: ... cloned into pET11a vector and included a C-terminal His6-tag (GenScript, USA). The AncCP-6An was also designed similarly to the caspase-6 CT (constitutive two-chain ...
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bioRxiv - Genetics 2022Quote: ... rabbit anti-OLLAS tag pre-absorbed against untagged animals 1:150 (Genscript, #A01658), mouse anti-FLAG pre-absorbed against untagged animals 1:400 (Sigma, ...
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bioRxiv - Biophysics 2022Quote: ... for 2 minutes followed by 20 μM biotinylated FLAG-tag antibody (A01429, GenScript) for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... leaving two extra GS residues between the tag and the M125 residue (GenScript). Wild-type and the associated LonP1 mutants were expressed in BL21 (DE3 ...
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bioRxiv - Genomics 2023Quote: CUT&Tag was performed with mouse anti-HA antibodies (1:100, Genscript #A01244), rabbit anti-H3K4me3 antibodies (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... hAPN with a C-terminal Flag tag was cloned into pcDNA3.1+ by GenScript. HEK293T cells seeded at 16E6 cells in 100 mm dishes coated with poly-D-Lysine and incubated overnight at 37 °C with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... FAM19A5 (NM_001252310.1) without tags was subcloned and inserted into the pcDNA3.1 vector (GenScript). The ectodomain (30-1263 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... all with a C-terminus FLAG tag epitope (all from Genscript, Piscataway, NJ). Transfections were performed using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: 15-mer peptides that are overlapping by 10 amino acids (AA) spanning the entire SARS-CoV-2 Spike protein (GISAID EPI_ISL_410713) were synthesized (Genscript) and pooled into 7 pools of approximately 40 peptides in each pool (Supplementary Table 1) ...
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bioRxiv - Immunology 2021Quote: ... and B.1.617.1 spike mutations (T95I, G142D, E154K, L452R, E484Q, D614G, P681R and Q1071H) was synthesised by GenScript into pCMV with a C-terminal foldon and avi tag followed by an octa-histidine tag ...
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bioRxiv - Microbiology 2020Quote: ... coli DHFR enzyme (FolA) was custom purified by Genscript (Piscataway, NJ). The enzymatic activity of DHFR with and without SCH-79797 treatment was measured using the Dihydrofolate Reductase Assay kit from Sigma ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coli codon optimization in the pET28b-(+) vector was ordered from GenScript. Then the SARS-CoV PLpro gene (ORF 1ab 1541-1855 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coli codon optimization in the pET28b-(+) vector was ordered from GenScript. Then MERS-CoV PLpro gene (ORF 1ab 1482-1803 ...
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bioRxiv - Molecular Biology 2020Quote: ... coli K12 nanR (UniProt accession-P0A8W0) was commercially synthesized by GenScript and subcloned into expression vector pET28a ...
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bioRxiv - Immunology 2022Quote: ... coli codon usage preference and synthesized by GenScript (Piscataway, NJ, USA), followed by subcloning into the pET41a using NdeI/XhoI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... coli expression (IDT) and a synthetic DNA construct was obtained (GenScript). The synthetic sglPP7 DNA was amplified using primers KC94 and KC116 and the resulting PCR product was gel purified (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coli codon optimization in the pET28b(+) vector was ordered from GenScript. The pET28b(+ ...
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bioRxiv - Microbiology 2020Quote: ... coli expression (Stothard, 2000) and synthesized by GenScript (New Jersey, USA) (E ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 (DE3) and cloned into pET-26b(+) vector (Genscript, USA) with a C-terminal 6X His-tag incorporated for immobilized metal affinity chromatography.
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bioRxiv - Immunology 2021Quote: ... One million of freshly isolated splenocytes were seeded into the precoated plates and stimulated with S1 and S2 peptides pools (GenScript) with a final concentration of 1 μg/ml of each peptide diluted in RPMI-1640 supplemented with 10% FBS and incubated for 48 hours at 37°C with 5% CO2 ...
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bioRxiv - Systems Biology 2022Quote: ... Primer and probes (see below in Table S1) were designed using Real-time PCR (TaqMan) Primer and Probes Design Tool (online tool) from GenScript and synthesized by BioTez ...
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bioRxiv - Cell Biology 2023Quote: All peptides used for binding assays (Data S1) were synthesized with a N-terminal 5-carboxyfluorescien (5-FAM) at >85% purity (GenScript); peptides used for competition studies did not have 5-FAM ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted fractions were analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE; Figure S1) gels run under denaturing conditions using SurePAGE Bis-Tris gels (GenScript) and MES-SDS running buffer (GenScript ...
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bioRxiv - Microbiology 2021Quote: ... codon-optimized SARS-CoV-2 ORF3 and E genes were synthesized by GenScript Biotech (Piscataway ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein complexes were detected by western blot with anti-His antibody (Genscript, A00186) and anti-Flag antibody (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... which were coated in anti-HIS antibody [Biotin] (GenScript A00613, mouse IgG1k clone 6G2A9) at 2.5 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... GrgA and mutants were detected using a monoclonal anti-His antibody (Genscript, Cat. A00186) and a mouse anti-GrgA antibody (35).