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Citations for GenScript :
401 - 450 of 819 citations for Glial fibrillary acidic protein GFAP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and human CDC73 sequences with an N-terminal Flag tag were synthesized and sequences confirmed by GenScript. The human ubiquitin sequence with an inserted N-terminal cysteine residue was synthesized by Eurofins ...
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bioRxiv - Biochemistry 2023Quote: ... The cDNA of human STK25 isoform 1 (RefSeq accession no. NM_001271977.2) was cloned into the pcDNA3.1+ vector (GenScript). The Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Cell Biology 2023Quote: The open reading frame of full-length human GPNMB (NM_001005340.2) cloned into pcDNA3.1-C-EGFP was purchased from GenScript. pcDNA3.1-GPNMB-EGFP-G375V ...
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bioRxiv - Biophysics 2023Quote: The codon optimized gene encoding the isoform 2 of full-length human HGSNAT was synthesized by GenScript. The synthesized gene was then cloned into the pEG BacMam expression vector (Addgene plasmid # 160683 ...
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bioRxiv - Immunology 2023Quote: ... pEF1-K6a (no tag) was constructed using a pUC57 vector encoding human K6a (codon-optimized by Genscript) as a PCR template to generate a full length human K6a DNA fragment terminated by a stop codon ...
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bioRxiv - Bioengineering 2021Quote: ... Codon-optimized cDNAs containing the effector protein RfxCas13d were synthesized (Genscript) and assembled into modified pET28 vectors by NEBuilder HiFi DNA assembly (E2621 ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were separated in 4-20% gradient precast PAGE gels (Genscript) and stained by Coomassie blue.
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bioRxiv - Biochemistry 2020Quote: ... Soluble protein was further purified via glutathione agarose chromatography (GenScript # L00206) with gravity flow at room temperature in fresh buffer B ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were precleared with protein A-sepharose beads (GenScript, Piscataway, NJ). Precleared supernatants were then incubated overnight at 4°C with 5μg of anti-NM IIA pAb or control rabbit immunoglobulin ...
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bioRxiv - Microbiology 2020Quote: ... and (3 µg) of GFP-tagged S protein (Genscript, MC 0101089). 48 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were resolved on 10% SDS-polyacrylamide gels (GenScript, M00666) and transferred to polyvinylidene fluoride membranes (Merck Millipore ...
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bioRxiv - Microbiology 2020Quote: ... centrifuged and purified by affinity chromatography with protein A resin (Genscript), and subjected to desalt in a composed buffer 50 mM NaPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... SMT (SUMO-like tag) fusion protein in a pET28a vector (Genscript). Quick Change mutagenesis was performed to generate the W611A mutant of hP13 ZnF5-WWE1-WWE2 ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were separated by 4– 12% gradient SDS-PAGE (GenScript) and blotted onto nitrocellulose or PVDF membranes ...
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bioRxiv - Evolutionary Biology 2022Quote: Protein G (5 μg/ml, 50 μl/well; Genscript, China, Z02007) was diluted to 5 μg/ml with PBS (0.01 M ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV (NP_828851.1) and MERS (AFS88936.1) Spike proteins were synthesized (GenScript) and cloned into pcDNA3.1 ...
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bioRxiv - Microbiology 2021Quote: LAD2 cells (3×105) were exposed to Spike-RBD protein (Genscript) (5 μg/mL) ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng/well Delta-S1 protein (GenScript, China) or 250 ng/well purified Delta-S protein in PBS and incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The DNA encoding this fusion protein was commercially synthesized (GenScript Inc) and cloned into pET15b bacterial expression vector at Nco I/BamH I cloning sites ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was transferred to a PVDF membrane using eBlot L1 (Genscript). Blocking was performed with 5% milk in PBST (PBS + 0.1% TritonX-100 ...
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bioRxiv - Microbiology 2022Quote: ... An anti-SARS-2 nucleocapsid protein rabbit antiserum (generated by GenScript) was used at a 1:1000 dilution to detect specific anti–SARS-2 immunoreactivity using the Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 N and S1 proteins were obtained from Genscript and Sino Biological.
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bioRxiv - Microbiology 2023Quote: ... The proteins were separated with an ExpressPlusTM PAGE gel (Genscript, M42012) and transferred to a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... smegmatis gene MSMEG_3935 encoding RafH protein was commercially synthesized from GenScript and cloned in the pET-28a(+ ...
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bioRxiv - Biochemistry 2023Quote: ... followed by staining using the eStain L1 Protein Staining System (GenScript). PAGE-MASTER Protein Standard Plus (GenScript ...
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bioRxiv - Immunology 2023Quote: CPG2 and β-Lac proteins were produced and purified by GenScript as previously described (7) ...
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bioRxiv - Physiology 2023Quote: ... Loaded protein samples were separated with Bis-Tris polyacrylamide gels (GenScript) and transferred to nitrocellulose membrane ...
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bioRxiv - Plant Biology 2024Quote: ... total protein extracts were loaded onto commercial precast gels (SurePage; GenScript) and immunoblotted with anti-GFP antibodies (Abcam Ab290 ...
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bioRxiv - Neuroscience 2024Quote: ... An additional plasmid encoding green fluorescent protein (GFP; pCAG-GFP; GenScript), was used to label successfully transfected cells ...
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bioRxiv - Biochemistry 2022Quote: The human SNX25 open reading frame (ORF) cloned into the pcDNA3.1+-C DYK was obtained from Genscript (NM_031953). This expresses a C-terminally tagged SNX25 (SNX25-FLAG ...
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bioRxiv - Molecular Biology 2019Quote: ... the human DDI2 sequence was amplified from pET16_wt_Ddi2 (Siva et al. 2016) using primers P45 and P46 (human DDI1 gene NM_001001711 was synthesized by GenScript and the coding sequence was amplified using primers P43 and P44) ...
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bioRxiv - Biochemistry 2020Quote: Full-length wild-type human ASPA cDNA with an N-terminal RGS6xHis-tag was expressed from pcDNA3.1 (Genscript). The C152W variant was generated by Genscript ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length sequences of human PLEKHA5 (NM_019012) and PLEKHA6 (XM_011509297) isoforms identified by Y2H screen were synthesized by Genscript (https://www.genscript.com/gene_synthesis.html ...
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bioRxiv - Biophysics 2020Quote: ... The codon optimized genes were synthesized for expression in human epithelium kidney cells (HEK293) (GenScript, Piscataway, NJ, USA), but were also found to prone to recombination upon insertion into pCDNA3.1 ...
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bioRxiv - Cancer Biology 2022Quote: ... and a portion was taken for replating (2×10^4 cells per replicate) with human (GenScript Z03034-50) or mouse (GenScript Z02767-10 ...
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bioRxiv - Biophysics 2022Quote: The full length human KCNQ2 construct (NCBI Reference Sequence: NP_742105.1; GI: 26051264) was synthesized (GenScript USA, Piscataway, NJ) and ligated between the BamHI and XbaI sites in the multiple cloning site of into the pGEM-HE vector ...
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bioRxiv - Cell Biology 2022Quote: ... ferritin L (Ft-L) made by using purified human ferritin L subunit as antigen by GenScript (Nanjing, China).
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bioRxiv - Microbiology 2020Quote: ... GenBank: MN908947) construct for preparation of RBD-based nanoparticle vaccine were human codon-optimized and synthesized by Genscript, and further cloned into mammalian expression vector VRC8400 with a N-terminal Kozak consensus sequence ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The plasmid encoding human FPR2 with an N-terminal SNAP-tag was obtained from Genscript (Piscataway, NJ, USA); it was constructed by replacing GLP1R in the previously described pcDNA3.1(+)-Flag-SNAP-GLP1R plasmid [26] with human FPR2 ...
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bioRxiv - Cancer Biology 2022Quote: Human RET-GFP and RET712-1114-GFP were cloned by PCR using a cDNA clone (GenScript Biotech; NM_020975.6) as template ...
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bioRxiv - Molecular Biology 2020Quote: Full-length wild-type human FLCN cDNA carrying an N-terminal RGS6xHis-tag was expressed from pcDNA3.1 (Genscript). USP7 was expressed with an N-terminal myc-tag from pcDNA3.1 (Genscript) ...
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bioRxiv - Genetics 2020Quote: ... and CH3 genes of human immunoglobulin isotype IgG1 were added to the heavy chain variable region (Genscript, USA). Vectors that encoded the anti-FAM19A5-IgG1 antibody were transfected into HEK293F cells and the recombinant antibody was purified using Protein A beads (RepliGen) ...
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bioRxiv - Molecular Biology 2022Quote: MFcS2: Modified human ACE2 (Sequence-Supplementary Material S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid encoding human CB2 with three amino-terminal HA-tags was custom-synthesized by GenScript (Rijswijk, Netherlands). The plasmids encoding human C5a1 ...
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bioRxiv - Cell Biology 2023Quote: The full length human arpin sequence cloned into the pET-32a(+) expression vector was from GenScript (Piscataway, NJ). The Arpin-pET-32a(+ ...
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bioRxiv - Biochemistry 2024Quote: The APN ectodomain from galline (Genbank ACZ95799.1) and human residues 66-967 was cloned into pcDNA3.1+ plasmid by GenScript with an N-terminal mu-phosphatase signal peptide sequence and C-terminal short linker GGS ...
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bioRxiv - Immunology 2023Quote: ... 50 μl of phycoerythrin (PE)– conjugated human angiotensin-converting enzyme 2 (ACE2) (hACE2; 1 μg per milliliter; GenScript) was added to the well and incubated for 30 minutes at 37°C with agitation ...
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bioRxiv - Biophysics 2024Quote: The full length human KCNQ3 construct (NCBI Reference Sequence: NP_004510.1; GI:4758630) was synthesized (GenScript USA, Piscataway, NJ) and ligated between the BamHI and XbaI sites in the multiple cloning sites of into the pGEM-HE vector ...
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bioRxiv - Immunology 2024Quote: ... the light chain immunoglobulin variable region sequences were inserted into the human kappa constant region sequence (Genbank accession number OM584289.1) and synthesised into mammalian vector pcDNA3.1 (Genscript). Human J chain was also synthesised into mammalian vector pcDNA3.1 ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides encoding residues 166–181 of human CHMP6 or various truncations of HCMV pUL71 were purchased from Genscript at >95% purity and the dry peptides were resuspended in ITC buffer to the desired concentration ...