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Citations for GenScript :
401 - 450 of 587 citations for Mouse ZNF583 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... This synthetic DNA was cloned into the DIs vector plasmid pSMART-DIs-L3-GPTF (purchased from GenScript, Nanjing, China), which harbors the Escherichia coli gpt gene (encoding xanthine-guanine phosphoribosyltransferase ...
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bioRxiv - Synthetic Biology 2021Quote: Coding sequences of all tested IspG proteins were ordered codon optimized and cloned in a pET28a(+) plasmid by Genscript. A Tobacco etch virus (TEV ...
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bioRxiv - Synthetic Biology 2021Quote: The human TDF HMG-box (hereinafter called TDF) was cloned into the plasmid pET-24c (+) (KanR) by GenScript (USA) using the sequence from the position 313 to 528 of the mRNA ...
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bioRxiv - Immunology 2020Quote: ... plasmids encoding cDNAs for the heavy and light chain sequences of 101F,64 MPE8,49 and DS747 were synthesized (GenScript), and cloned into vectors encoding human IgG1 and lambda or kappa light chain constant regions ...
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bioRxiv - Immunology 2021Quote: ... The full-length native chicken ovalbumin (OVA) gene inserted in a pcDNA3.1+ plasmid (clone ID: OGa28271) was purchased from GenScript. Gene inserts from pcDNA3.1+ plasmids were cloned into the pAM2AA backbone incorporating the liver-specific human α-1 antitrypsin promoter and human ApoE enhancer flanked by AAV2 inverted terminal repeats ...
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bioRxiv - Cancer Biology 2022Quote: pcDNA3.1+C-HA Trp53 (OMu22847) and pcDNA3.1+C-FLAG Ppia (OMu14516) plasmids used in mammalian over-expression experiments were purchased from Genscript. The detailed information regarding plasmid constructions is available by request ...
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bioRxiv - Bioengineering 2022Quote: ... by means of overhang PCR and was cloned via traditional restriction/ligation into a pET28a(+) plasmid (ordered from GenScript) coding for the C-terminal flexible linker and small BiT (SB ...
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bioRxiv - Microbiology 2022Quote: The pspA1 and pspA2 genes of SF35 were synthesized and cloned in pET-28a(+)-Tev plasmid (GenScript Biotech (Netherlands) BV) ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 spike constructs were generated and cloned into the pcDNA3.1 plasmid backbone using KpnI and BamHI restriction enzyme cloning by GenScript Biotech (Piscataway ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... thus introducing the cloning artifact ‘GPLGS’.37 The pGEX-6p-1-GST-OSBP377-807 plasmid was obtained from Genscript. The pET22b-His6-STARD166-284 plasmid was a gift from James H ...
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bioRxiv - Neuroscience 2023Quote: ... An IgG2b expression plasmid encoding the K89/34 IgG2b R-mAb was generated to order by Genscript (https://www.genscript.com/) by replacing the mouse IgG2a (ψ2a ...
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bioRxiv - Bioengineering 2023Quote: The L1 gene fragment sequences of human papillomavirus (HPV) types 16 and 18 were incorporated into the pCDNA3.1(+) plasmid by Genscript. To amplify the specific regions of interest ...
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bioRxiv - Cell Biology 2023Quote: DNA sequences used for plasmid construction were either amplified from the cDNA of HEK293T cells or purchased from GenScript and WZ Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... The following plasmids were used in this study: FLAG tagged ORFs in pcDNA3.1+/C-(K)DYK were purchased from Genscript: CDK1 (NM_001786.5) ...
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bioRxiv - Cell Biology 2024Quote: ... The PLCε-FLAG-P2A-mCherry plasmid was synthesized and subsequently cloned into a pCMV-script EX vector by GenScript. For rescue experiments involving PLCε KO chromaffin cells ...
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bioRxiv - Cell Biology 2021Quote: ... and a mouse monoclonal antibody to detect GAPDH (clone 3B1E9, GenScript A01622–40) were used at a dilution of 1:1,000 in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Horseradish peroxidase (HRP) labeled-mouse anti-human IgG-Fc specific (GenScript No. A01854) diluted 1:10,000 in PBST was added (100μl/well ...
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bioRxiv - Microbiology 2021Quote: ... Mouse anti-CodY IgG monoclonal antibody (IgG) was generated and purified (Genscript, USA).
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bioRxiv - Microbiology 2022Quote: ... The primary antibody for Western blot is Mouse-anti-His mAb (GenScript, Cat.No.A00186). The concentration was determined by BCA protein assay with BSA as a standard ...
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bioRxiv - Physiology 2021Quote: The sequence encoding mouse like-acetylglucosaminyltransferase-1 (Large1) was synthesized (Genscript, Piscataway, NJ) and cloned into the AAV backbone under the transcriptional control of the ubiquitous CMV promoter ...
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bioRxiv - Immunology 2022Quote: ... then 3.5μL of 1 mg/ml normal mouse IgG (mIgG) (GenScript, Cat# A01007) was added ...
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bioRxiv - Biochemistry 2022Quote: The sequence encoding mouse like-acetylglucosaminyltransferase-1 (Large1) was synthesized (Genscript, Piscataway, NJ) and cloned into the AAV backbone under the transcriptional control of the ubiquitous CMV promoter ...
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bioRxiv - Biochemistry 2022Quote: ... The sequence encoding mouse like-acetylglucosaminyltransferase-1 (Large1) was synthesized (Genscript, Piscataway, NJ) and cloned into the AAV backbone under the transcriptional control of the muscle-specific MCK promoter (gift from Jeff Chamberlain) ...
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bioRxiv - Biophysics 2023Quote: ... human/mouse codon-optimized genes coding for the identified ChRs were ordered (GenScript, Piscataway ...
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bioRxiv - Genomics 2023Quote: CUT&Tag was performed with mouse anti-HA antibodies (1:100, Genscript #A01244), rabbit anti-H3K4me3 antibodies (1:100 ...
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bioRxiv - Biochemistry 2023Quote: Sequences encoding mouse WT and site-mutated DAG1 were synthesized (GenScript, Piscataway, NJ) and cloned into adeno-associated virus 2/9 (AAV2/9 ...
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bioRxiv - Cell Biology 2024Quote: Protein lysates were incubated with 1 µg mouse anti-V5 antibody (Genscript A01724) for 2 h at 4°C ...
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bioRxiv - Genetics 2024Quote: ... HRP-conjugated mouse monoclonal antibody against FLAG was obtained from GenScript (Cat# A01428).
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bioRxiv - Plant Biology 2020Quote: ... the 500 bp 3’sequences from AT1G76560 (wild-type and mutated) and AT1G03160 flanked by attR1 and attR2 sites were synthesized and inserted in the pUC57 plasmid by Genscript. The DNA insert was then shuttled by Gateway cloning into the dual-luciferase vector nLucFlucGW (Genbank MH552885 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and cloned into plasmid pBAD24 (79) between EcoRI and SphI restriction sites with an NdeI site at the start codon by GenScript. SNARES involving ClyA ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 300–600 bp homology arms were synthesized as gBlocks (IDT) and cloned into a pUC19 plasmid inhouse or purchased directly from Genscript Biotech ...
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bioRxiv - Cancer Biology 2021Quote: ... XPA expression plasmids contain full-length human XPA (NM_000380) with the indicated mutations in the pcDNA3.1(+) backbone (GenScript custom order).
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bioRxiv - Cell Biology 2020Quote: ... The resulting intermediate 2 was complemented with a copia promoter-blasr cassette after amplifying the cassette using JB011 and JB012 from a plasmid with a corresponding synthetic DNA insert obtained from GenScript. The resulting PCR fragment was digested with NcoI and SpeI ...
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bioRxiv - Biochemistry 2022Quote: ... the expression plasmid encoding the sequence of 3D7 PfAMA1(DI+DII) (104-438) was obtained commercially from GenScript (NI, USA) having N-terminal hexa-His tag along with one TEV-protease cleavage site ...
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bioRxiv - Microbiology 2019Quote: Mammalian expression plasmids (pcDNA3.1+/C-(K)DYK) for HLA-DRA (NM_019111) and HLA-DRB1 (NM_001243965) were purchased from GenScript (Piscataway, NJ; USA). HEK293T/17 cells at sub-confluence in 6-well plates or 100 mm dishes were transfected with HLA-DRA plasmid or HLA-DRB1 plasmid or a 1:1 combination of both using the Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The HNF4A-targeting gRNA is from the genome-scale CRISPR knock-out (GeCKO) v2 library [106] (purchased as lentiviral plasmid from Genscript) and the FMR1-targeting gRNA from the Jaenisch lab was obtained from Addgene (pgRNA-CGG ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid encoded an N-terminal 8X His-SUMO-tagged hNatD in the pET-21a vector was obtained from Genscript. Library of Pharmaceutically Active Compounds (LOPAC ...
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bioRxiv - Microbiology 2020Quote: ... were synthetized and cloned in plasmid pSG1-RFP (between the AscI and NotI sites of the multiple cloning sites) by Genscript Corp ...
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bioRxiv - Synthetic Biology 2021Quote: All gBlocks fragment containing 5 sgRNA expression cassettes with high fidelity four-base overhang pair2 after cutting with type IIS restriction enzyme BbsI restriction enzyme were designed and directly sent to be synthesized into PUC57 cloning plasmid by GenScript. Two oligos with BbsI cutting sites were annealed and cloned into backbone vector with CMV promoter drive fluorescent protein expression using SpeI-HF ...
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Comparison of the neuraminidase antigenicity in recently circulating influenza A and vaccine virusesbioRxiv - Microbiology 2021Quote: ... The genes corresponding the NAs from the following strains were synthesized and inserted into the pHW2000 plasmid [47] by Genscript: A/H1N1/Guangdong-Maonan/SWL1536/2019 (N1-GD19) ...
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bioRxiv - Systems Biology 2021Quote: ... The +1 nucleotide was mutated to all other nucleotides (G, C or T) and these 3 mutant plasmids were synthesized into DNA oligos and cloned by Genscript.
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bioRxiv - Cell Biology 2021Quote: ... the base mNeon-pDM304 expression plasmid for N-terminal fusions was generated by restriction enzyme cloning a codon-optimized synthesized mNeon gene (GenScript) into the extrachromosomal expression plasmid pDM304 (Veltman et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The base Scarlet I-pDM304 expression plasmid for C-terminal fusions was generated by restriction enzyme cloning a codon-optimized synthesized Scarlet I gene (GenScript) into the extrachromosomal expression plasmid pDM304 (Veltman et al. ...
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bioRxiv - Biophysics 2021Quote: pET-DUET expression plasmids containing the genes for barnase and barstar under the control of their own T7 promoter were obtained from GenScript Biotech Corporation (Piscataway ...
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bioRxiv - Biophysics 2021Quote: A plasmid expressing mature OmpA without the 22 amino acid signal sequence in the pET303 vector was purchased from Genscript for cloning of the modified loop constructs ...
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bioRxiv - Bioengineering 2022Quote: ... knock-out was constructed by cloning a chloramphenicol marker to the pUC57 plasmid containing the sequences flanking acr1 (here named as pJL8, purchased from Genscript). The plasmid pJL10 was constructed by cloning the PT5-acr1-kanr cassette (Luo et al. ...
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bioRxiv - Biochemistry 2022Quote: The gene encoding SARS-CoV-2 NSP3 Mac1 (residues 3-169) was cloned into a pET-22b(+) expression plasmid with a TEV-cleavable N-terminal 6-His tag (Genscript). The protein was expressed and purified as described previously (14).
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bioRxiv - Immunology 2022Quote: ... Matched pairs of antibody VH and Vλ/Vκ sequences were commercially cloned into plasmids containing an IgG1 or relevant light chain backbone and expressed as recombinant antibody (Genscript). mAbs were also expressed in-house by transient transfection of Expi293 cells (Gibco ...
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bioRxiv - Neuroscience 2020Quote: TDP-43 BiFC constructs: Synthesis and subcloning of the gene fragments into pCS2+ plasmids were performed by GenScript (NJ, USA). Wild-type human TDP-43 was N-terminally fused to either VN155 or CC155 via a (GGGS)3 flexible linker ...
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bioRxiv - Microbiology 2021Quote: ... coli K-12 MG1655 thymidylate kinase alleles (WT, Q45P, and A69T) were amplified and cloned into the plasmid expression vector pRSFDuet-1 (GenScript). Expression is under control of the T7 lac promoter ...