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Citations for GenScript :
351 - 400 of 656 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The codon-optimized sequence of His-tagged E2 (Genscript Biotech) based on an HCV variant of the genotype 1a H77 strain (Genbank ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoblotting was performed as reported (20) using anti-His (GenScript) or anti-carrot FBA (14 ...
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bioRxiv - Plant Biology 2019Quote: ... coli and affinity purified using Glutathione Resin (GenScript) and TALON® Metal Affinity Resin (Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... coli expression in pET-20b(+) plasmid from GenScript. We transformed One Shot™ BL21(DE3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli and sub-cloned into pET28a(+) vectors (Genscript). Each ferredoxin was expressed in BL21(DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... coli was obtained in pET22b vector from Genscript. LceB without the secretion signal was PCR amplified off the plasmid to include restriction sites NdeI and BamHI ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using Ni-NTA chromatography (Genscript). AiEvo2 protein was complexed with guide RNA (IDT ...
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bioRxiv - Bioengineering 2022Quote: ... coli K5 and codon optimization was done (GenScript). The primers used for amplifying different gene fragments and the associated restriction sites are shown in Table 3 ...
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bioRxiv - Immunology 2020Quote: ... labeling with 1:200 biotinylated anti-Strep tag antibody (GenScript) was followed by 1:500 BrilliantViolet 421 conjugated with Streptavidin (Biolegend) ...
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bioRxiv - Molecular Biology 2019Quote: ... A 3xFLAG-2xHA tag was synthesized by GenScript (Piscataway, USA) and PCR amplified using primer pair MT-A70_HA-F/HA_R for PfMT-A70 and YTH1_HA-F/HA_R for PfYTH (Supplementary Table 9) ...
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bioRxiv - Immunology 2023Quote: ... THETM V5 Tag antibody (both at 1:5000 dilution, GenScript), and CLIPA8 primary antibody (1:5000 dilution ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were transfected with pcDNA3.1-spike-V5 with or without pcDNA3.1-GALNTs-FLAG (Genscript) using Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... vaccines consisted of either SARS-CoV-2 Spike RBD WH-01 protein (GenScript; cat# Z03483) or SARS-CoV-2 WH-01 Spike protein (Acro Biosystems ...
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bioRxiv - Immunology 2021Quote: ... cells were immunostained using a rabbit anti-spike monoclonal antibody (mAb BS-R2B12, GenScript A02058), anti-rabbit IgG peroxidase conjugate ...
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bioRxiv - Microbiology 2021Quote: LAD2 cells (3×105) were incubated with Spike-RBD protein (5 μg/mL, Genscript, Z03483) in adherent buffer (1mM CaCl2 ...
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bioRxiv - Immunology 2022Quote: ... Delta B1.617.2 clone was generated by gene synthesis with a codon-optimized Spike ORF (GenScript). The Omicron ORF was amplified from an RNA as described for protein production with primers listed in Table 3 ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles for spike transduction were generated by transfecting 293T cells with pMD2.G (Genscript), psPAX2 (Genscript ...
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bioRxiv - Cell Biology 2022Quote: Human His-CLASP1 (NM_015282.2) in pFastBacHT vector was purchased from Genscript. The cDNA encoding full-length human CLASP2α was purchased from Dharmacon (Accession ...
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bioRxiv - Biochemistry 2023Quote: ... His-ERK was further purified using 1 mL glutathione resin (GenScript) to remove any residual GST-tagged MKK1.
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bioRxiv - Biochemistry 2020Quote: ... coli codon optimization was ordered from GenScript (Piscataway, NJ). The expression and purification of SARS-CoV-2 Mpro was described as previously.11
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bioRxiv - Neuroscience 2020Quote: ... Coli ArcticExpress and purified from inclusion bodies by GenScript. Recombinant RTN3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli or yeast and synthesized by GenScript (Piscataway, NJ) or Twist Biosciences (San Francisco ...
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bioRxiv - Cell Biology 2021Quote: ... coli lysates using glutathione-agarose or -sepharose (Sigma; GenScript) and Talon metal affinity resin (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: ... coli and synthesized (Genscript Inc, Integrated DNA Technologies Inc) with NdeI site on the 5’ end and a termination codon (TAA or TAG ...
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bioRxiv - Immunology 2022Quote: ... coli and purchased in the pUC57 vector from GenScript (GenScript ...
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bioRxiv - Cell Biology 2020Quote: ... coli optimal codons and cloned into pUC57 by GenScript USA, ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli codon optimization were ordered from GenScript (Piscataway, NJ). The Mpro gene was then subcloned into pE-SUMO vector as described previously7,8 ...
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bioRxiv - Microbiology 2021Quote: ... coli AW1.7 were also synthesized and cloned by Genscript. All synthesized sequences are presented in Table S5 ...
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bioRxiv - Microbiology 2023Quote: ... coli KTE188 (Genbank: ANTE01000038)26 was synthesized by Genscript together with its native promoter and terminator sequences and cloned into plasmid pSG1 35 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli and synthesized in vitro by GenScript (Piscataway, NJ). The resulting gene cassettes were digested with restriction enzymes and tandemly ligated into a custom Hb expression vector (Natarajan et al. ...
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bioRxiv - Immunology 2021Quote: Biotinylated SARS-CoV-2 S1 protein and biotinylated SARS-CoV-2 N protein were purchased from GenScript. The biotinylated proteins were combined with different streptavidin (SA ...
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bioRxiv - Immunology 2020Quote: ... were coated in 1 carbonate buffer (0.1 M at pH 9.6) with 1.0 ug/ml S1 protein (GenScript). The plates were incubated overnight at 4°C in a humidified chamber and then blocked in PBS plus 0.05% Tween 20 (PBST ...
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bioRxiv - Cell Biology 2021Quote: cDNA constructs with the indicated epitope tags were synthesized (Genscript, USA) and provided as entry clones ...
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bioRxiv - Biochemistry 2021Quote: ... SMT (SUMO-like tag) fusion protein in a pET28a vector (Genscript). Quick Change mutagenesis was performed to generate the W611A mutant of hP13 ZnF5-WWE1-WWE2 ...
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bioRxiv - Biochemistry 2020Quote: ... containing a TEV protease cleavage site and a His6 tag (Genscript). All constructs were verified with DNA sequencing of both strands.
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bioRxiv - Molecular Biology 2023Quote: ... Peptides containing an N-terminal biotin tag were purchased from GenScript and dissolved according to manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2023Quote: ... a rabbit anti-Strep-tag IgG antibody (Genscript, Piscataway, NJ, USA) and a goat anti-rabbit IgG Alexa 488 conjugate as secondary antibody were used ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were anti-strep tag (GenScript, A01732, 1/1000), anti-PHF21A (in house ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding human codon-optimized spike genes with the desired mutations were purchased (GenScript, Piscataway, NJ). Supernatants containing pseudoviruses were collected 48 h post-transfection ...
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bioRxiv - Immunology 2021Quote: ... The plasmid encoding the Spike of theB.1.1.7 variant was codon-optimized and synthesized by Genscript.
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bioRxiv - Immunology 2022Quote: ... vaccines consisted of 10 µg SARS-CoV-2 Spike RBD WH-01 protein (GenScript, cat# Z03483), combined with 1 nmol AMP-CpG-7909 (AMP-CpG ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 Spike gene from plasmid pUC57-2019-nCoV-S-Hu (GenScript, Piscataway, USA) was truncated in its cytoplasmic tail of 19 amino acids ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein (ECD) and RBD were purchased from GenScript (Piscataway, NJ, USA).
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2-RBD-his protein was purchased from GenScript (GenScript, Nanjing), and GPC5-his protein was purchased from R&D (Minneapolis ...
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bioRxiv - Microbiology 2019Quote: ... Wild-type MreB and a series of amber codon mutants (Table S1) were synthesized by Genscript (Piscataway, NJ) and used to replace the IPTG-inducible YFP to create a plasmid encoding Plac-MreB and Para-AiMB (pMreBXL1-26) ...
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bioRxiv - Molecular Biology 2022Quote: MFcS2: Modified human ACE2 (Sequence-Supplementary Material S1) was codon optimized for HEK293 expression and synthesized from Genscript USA in pUC57 vector ...
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bioRxiv - Plant Biology 2020Quote: ... coli (Supplementary Table 4) and synthesized by GenScript (Piscataway, NJ). The Arabidopsis THI4 sequence was the native cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... coli expression (Supplementary Table 3) and synthesized in vitro (Genscript). For protein expression ...
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bioRxiv - Microbiology 2020Quote: ... coli situated in pET28b were purchased from Genscript (Piscataway, NJ). Sequence data for these constructs are provided as supplemental data ...
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bioRxiv - Biochemistry 2024Quote: ... coli expressed saposins: We purchased from Genscript (Piscataway, NJ, USA) the codon-optimized SapB and SapD (UniProt P07602 ...