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Citations for GenScript :
301 - 350 of 910 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... The coding sequences of both domains were optimized for codon usage of the host (E. coli BL21) using OptimumGeneTM (GenScript, USA) and a His-Tag was added at the carboxyl end for further purification ...
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bioRxiv - Microbiology 2022Quote: ... Heterologous expression was detected by electroblotting SDS-PAGE Tris-glycine gels onto nitrocellulose membranes and immunostaining with primary rabbit anti-His-tag antibody and secondary goat anti-rabbit IgG phosphatase alkaline conjugated antibody (GenScript, Piscataway, NJ, USA) (Suppl ...
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bioRxiv - Bioengineering 2024Quote: Genes encoding the designed protein sequence were synthesized and cloned into pET-24a(+) E.coli plasmid expression vectors (Genscript, C-terminal 6X His tag). Plasmids were then transformed into chemically competent BL21(DE3 ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL of reporter (1000 nM, GenScript Biotech Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... of SARS-CoV-2 (D614) and the ectodomain of human angiotensin converting enzyme 2 (ACE2; residue 1-615) were synthesized by GenScript (Piscataway, NJ). The S gene was fused with a C-terminal twin Strep tag [(GGGGS)2WSHPQFEK(GGGGS)2WSHPQFEK)] and cloned into a mammalian cell expression vector pCMV-IRES-puro (Codex BioSolutions ...
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bioRxiv - Immunology 2023Quote: ... expressing spike or STX-N_pLenti (Genscript) expressing nucleocapsid using Lipofectamine 3000 according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... expressing spike or STX-H3_pGenlenti (Genscript) at a ratio of 5:5:1 using Lipofectamine 3000 according to the manufacture’s instruction ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using polyethylenimine (PEI ...
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bioRxiv - Microbiology 2019Quote: ... and 1 µg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and F4 (RAPH1 aa 868-1062) constructs were purchased from GenScript. Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... coli (GenScript) and included a Gly-Ser linker (encoded by an in-frame BamHI site ...
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bioRxiv - Biochemistry 2022Quote: ... coli (Genscript) and subsequently cloned into pRSF-Duet1 (Novagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... coli (GenScript) and placed in the pSY5 vector3,26 ...
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bioRxiv - Biophysics 2022Quote: ... coli (GenScript). In each case ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (GenScript) and cloned into pET28-Sumo3 vector (EMBL ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Genscript) and subcloned using ligation-independent cloning into pMCSG7 (61) ...
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bioRxiv - Plant Biology 2024Quote: ... coli (Genscript) and cloned into the pET-22b expression vector (Novagen ...
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bioRxiv - Biochemistry 2021Quote: pcDNA3.1-spike and pcDNA-3.1-spikeΔfurin (Genscript) were fused with V5 epitope at the C-terminus to make pcDNA3.1-spike-V5 and pcDNA-3.1-spikeΔfurin-V5.
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bioRxiv - Microbiology 2022Quote: ... Mutant spike plasmids were produced by Genscript. Pseudoviruses were mixed with 8 serial 4-fold dilutions of sera or antibodies in triplicate and then added to monolayers of 293T-hACE2 cells in triplicate ...
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bioRxiv - Biochemistry 2023Quote: ... The gene encoding for the GAF2 domain from All1280 of Nostoc PCC 7120 (NCBI protein ID BAB73237.1, UniProtKB Q8YXD3, amino acids 562-727) was ordered from Genscript (codon optimized for E. coli) in the pUC18 cloning vector ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-RAPH1F2 construct (RAPH1 aa 535-868) was purchased from GenScript.
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bioRxiv - Cell Biology 2023Quote: The cDNA sequence for the 151-aa of ORF2 were synthesized (Genscript) with FLAG tag sequence inserted downstream the ATG start codon and cloned to pShuttle 2 vector ...
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bioRxiv - Immunology 2024Quote: ... 293F cells were transfected with plasmids containing Spike protein (ATUM plasmid pD2528-CMV with insert QHD43416.1) and Spike-2P protein (site-directed mutagenesis to generate the 2P mutation on the plasmid containing Spike protein, GenScript) by 293fectinTM transfection reagent (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Wells were washed 3 times in PBS and incubated with 1:1000 anti-His HRP antibody (GenScript, A00612, Lot. 19K001984) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Treatments with either Spike (100nM, Cat#Z03485, Genscript) or the NRP-1 inhibitor EG-00229 (30µM ...
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bioRxiv - Biochemistry 2022Quote: ... from various snake species were selected from NCBI databases and cloned into a mammalian expression vector containing a C-terminal Avi™ tag followed by a WELQut™ cleavage tag and a rabbit Fc tag by Genscript. Plasmid DNA was prepped and co-transfected with a plasmid expressing the BirA enzyme for in vivo biotinylation into Expi293 cells using FectoPRO® (Polyplus Transfection) ...
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bioRxiv - Biophysics 2019Quote: ... coli expression (Genscript) and further subcloned in pET28a vector using NDE1 and Xho1 sites ...
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bioRxiv - Biochemistry 2020Quote: ... coli (Genscript, NJ) were incorporated into the multi-cloning site in the pET21a(+ ...
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bioRxiv - Biochemistry 2020Quote: ... coli by GenScript and subcloned into a pET30 vector (Novagen ...
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bioRxiv - Biochemistry 2019Quote: ... coli by Genscript Corporation ...
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bioRxiv - Microbiology 2020Quote: ... coli lysate (Genscript) at final concentration of 10 mg/mL to block anti-E ...
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bioRxiv - Biochemistry 2023Quote: ... coli by GenScript. These genes were prefixed with HRV 3C cleavage sites and cloned into the corresponding vectors between the NdeI and XhoI sites ...
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bioRxiv - Microbiology 2023Quote: ... coli by GenScript. This gene was cloned into the NdeI and XhoI cut sites of a pET28a(+ ...
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bioRxiv - Microbiology 2023Quote: ... coli by Genscript. The sequence encoding the C-terminal LysM domain (401-460 ...
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bioRxiv - Bioengineering 2022Quote: ... coli codons (Genscript). A second version ...
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bioRxiv - Cancer Biology 2023Quote: ... coli (GenScript Biotech) and cloned into the modified pET-49b(+ ...
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bioRxiv - Cancer Biology 2023Quote: ... coli (GenScript Biotech). Cloning and expression of 367-771 fragment (413 aa ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SARS CoV-2 Mpro and PLpro expression plasmids pcDNA3.1 SARS2 Mpro and pcDNA3.1 SARS2 PLpro was ordered from Genscript (Piscataway NJ) with codon optimization.
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 sequences (Genbank accession number MN908947) were codon-optimized for Chinese Hamster Ovary (CHO) cells and synthesized by GenScript. Within the construct ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His-HRP (Genscript, A00612), Avidin-HRP (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... Anti-His-HRP (Genscript, A00612), Avidin-HRP (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (1:4,000) (GenScript), anti-GFP (1:10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged Enterokinase (Z03004, Genscript) was added to the dialyzed ...
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bioRxiv - Immunology 2020Quote: ... anti-S2 (SinoBiologicals #40590-D001) and anti-NC (Genscript #HC2003). Human and rhesus IgG antibodies were both detected in these calibration assays using biotinylated affinity-purified goat anti-human IgG γ chain polyclonal antibody (SBA #2048-08) ...
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bioRxiv - Microbiology 2023Quote: ... Dabie Bandavirus (DBV) glycoprotein Gn gene (GenBank NC_018138.1) was codon-optimized for human codon usage (Genscript) and cloned into the expression vector ...
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bioRxiv - Biochemistry 2023Quote: ... and α-CBP tag (GenScript, catalog # A00635 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged AtxA was detected using anti-His antibody (GenScript USA Inc., Piscataway, NJ, USA). RNA polymerase subunit β was used as a loading control and detected using anti-RNAP antibody (Thermo fisher ...
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bioRxiv - Biochemistry 2021Quote: The codon optimized cDNA of full-length spike (Genscript) was amplified by PCR and digested by HindIII and NotI ...