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Citations for GenScript :
251 - 300 of 740 citations for SARS CoV 2 Spike Glycoprotein S1 RBD Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... PLA2G7 and SIAE from Chinese hamster ovary (CHO) cells were expressed in CHO-3E7 cells and purified (>90% purity) by GenScript (Piscataway, NJ).
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bioRxiv - Immunology 2020Quote: ... The S1-N-terminal domain (S1-NTD, amino acids 16-318) was custom synthesized by GenScript. Each protein was expressed with an N-terminal His6-Tag to facilitate purification ...
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bioRxiv - Biophysics 2022Quote: ... for 2 minutes followed by 20 μM biotinylated FLAG-tag antibody (A01429, GenScript) for 30 minutes ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: Recombinant SARS-CoV-2 wild-type S protein RBD-HRP fusion protein (RBD-HRP protein, cat. no. Z03594) and hACE2 protein (cat. no. Z03516) were purchased from GenScript Korea Ltd ...
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bioRxiv - Microbiology 2020Quote: ... interaction between CD147 and SP (RBD) (GenScript, China) was detected using Kinetic Analysis/Concentration Series/Direct Binding mode ...
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bioRxiv - Biochemistry 2020Quote: ... of SARS-CoV-2 (D614) and the ectodomain of human angiotensin converting enzyme 2 (ACE2; residue 1-615) were synthesized by GenScript (Piscataway, NJ). The S gene was fused with a C-terminal twin Strep tag [(GGGGS)2WSHPQFEK(GGGGS)2WSHPQFEK)] and cloned into a mammalian cell expression vector pCMV-IRES-puro (Codex BioSolutions ...
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bioRxiv - Immunology 2023Quote: ... expressing spike or STX-N_pLenti (Genscript) expressing nucleocapsid using Lipofectamine 3000 according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... expressing spike or STX-H3_pGenlenti (Genscript) at a ratio of 5:5:1 using Lipofectamine 3000 according to the manufacture’s instruction ...
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bioRxiv - Immunology 2021Quote: ... by subcutaneous injection with 50μL S1 (Genscript) (50μg/rat ...
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bioRxiv - Microbiology 2024Quote: ... Table S1) and cloned into pUC57 (GenScript). A previously published gfp sequence (Takacs et al. ...
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bioRxiv - Immunology 2023Quote: A commercially available kit to quantify the ability of the three pAbs to neutralize the binding of RBD to ACE-2 was obtained from GenScript, Piscataway NJ (kit #L00847) ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using polyethylenimine (PEI ...
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bioRxiv - Microbiology 2019Quote: ... and 1 µg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Immunology 2023Quote: ... and 1 μg of vesicular stomatitis virus-G glycoprotein expressing plasmid pMDG (Genscript) using Fugene 6 transfection reagent (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Immunizations were done on eight-to twelve-week-old H2L2 mice interperitoneally with 50-100 μg of a recombinant SARS-CoV2 Spike RBD319-591-Fc fusion protein generated from sequence from the original Wuhan seafood market pneumonia virus isolate (GenBank Accession# MN908947) and cloned in-frame into pcDNA vectors containing human IgG1 and mouse IgG2a Fc tags (GenScript USA Inc., Piscataway, NJ). Each mouse received a prime followed by 2 boosts ...
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bioRxiv - Immunology 2021Quote: Antibodies inhibiting virus binding to host cell was measured using a commercial RBD-human angiotensin-converting enzyme 2 (hACE2) binding inhibition assay called cPASS™ (GenScript). As per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: RBD protein expressed with AviTag was purchased from GenScript. Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity) ...
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bioRxiv - Immunology 2021Quote: ... The B.1.429 RBD gene was synthesized by GenScript into pCMVR with the same boundaries and construct details with a mutation at L452R ...
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bioRxiv - Bioengineering 2021Quote: RBD protein expressed with AviTag was purchased from GenScript. Site-specific biotinylation of the AviTag was performed using BirA Biotin-Protein Ligase Reaction kit (Avidity) ...
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bioRxiv - Cell Biology 2019Quote: ... and 10 μg repair plasmid (Genscript, Note S1), followed by electroporation using program B024 of Nucleofector™ 2b Device (Lonza #AAB-1001) ...
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bioRxiv - Biochemistry 2021Quote: pcDNA3.1-spike and pcDNA-3.1-spikeΔfurin (Genscript) were fused with V5 epitope at the C-terminus to make pcDNA3.1-spike-V5 and pcDNA-3.1-spikeΔfurin-V5.
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bioRxiv - Microbiology 2022Quote: ... Mutant spike plasmids were produced by Genscript. Pseudoviruses were mixed with 8 serial 4-fold dilutions of sera or antibodies in triplicate and then added to monolayers of 293T-hACE2 cells in triplicate ...
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bioRxiv - Microbiology 2020Quote: ... the cDNA for PbChiB2 (Fig. S1) and PbChiB4 (Fig. S1) without the signal peptide was synthesized and cloned into plasmid pET-14b (GenScript, USA). Plasmids were used to transform E ...
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bioRxiv - Biophysics 2020Quote: ... His-tag and Strep-tag was codon optimized for mammalian expression (Genscript) and inserted into pFastBac1 (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His tag (Genscript) or anti-FLAG (Genscript ...
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bioRxiv - Genetics 2021Quote: ... we stained the yeast with recombinant CoV2 RBD-His6 (Genscript) at diminishing concentrations through the rounds (5nM ...
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bioRxiv - Immunology 2024Quote: ... 293F cells were transfected with plasmids containing Spike protein (ATUM plasmid pD2528-CMV with insert QHD43416.1) and Spike-2P protein (site-directed mutagenesis to generate the 2P mutation on the plasmid containing Spike protein, GenScript) by 293fectinTM transfection reagent (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... recombinant human ACE2-IgG-Fc fragments (r-hACE2-Fc) (GenScript, Z03516) were firstly incubated with the Protein-G agarose (Millipore ...
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bioRxiv - Immunology 2023Quote: ... Peptides (Table S1) were synthesised at >90% purity (GenScript), stored lyophilised at −80°C and then reconstituted in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Treatments with either Spike (100nM, Cat#Z03485, Genscript) or the NRP-1 inhibitor EG-00229 (30µM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SARS CoV-2 Mpro and PLpro expression plasmids pcDNA3.1 SARS2 Mpro and pcDNA3.1 SARS2 PLpro was ordered from Genscript (Piscataway NJ) with codon optimization.
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bioRxiv - Microbiology 2023Quote: ... Dabie Bandavirus (DBV) glycoprotein Gn gene (GenBank NC_018138.1) was codon-optimized for human codon usage (Genscript) and cloned into the expression vector ...
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bioRxiv - Biochemistry 2021Quote: RBD inhibition assay: HEK-293T cells stably expressing hACE2 (GenScript M00770) were seeded into a 24-well plate at an initial density of 6 x 104 cells per well ...
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding for RBDs of different sarbecoviruses were synthetized by Genscript and cloned in the phCMV1 vector ...
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bioRxiv - Biochemistry 2023Quote: ... and α-CBP tag (GenScript, catalog # A00635 ...
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bioRxiv - Biochemistry 2021Quote: ... and subcloned into pET-28a(+) vector (Genscript, supplementary table s1). Expression of the recombinant protein harbouring an N-terminal His6 tags was carried out in E ...
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bioRxiv - Systems Biology 2020Quote: ... The codon-optimized kmERG1 were ordered from GenScript (Table S1), and the PrimerSTAR HS polymerase was utilized for gene amplification through PCR ...
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bioRxiv - Microbiology 2020Quote: ... A human chimeric anti-S1 antibody (Genscript; 1:200 dilution) followed by an Alexa647-conjugated goat anti-human IgG (Jackson Laboratories ...
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bioRxiv - Biochemistry 2021Quote: The codon optimized cDNA of full-length spike (Genscript) was amplified by PCR and digested by HindIII and NotI ...
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bioRxiv - Immunology 2022Quote: ... or were created by spike gene synthesized by GenScript using the spike sequence in VRC7480 as template ...
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bioRxiv - Immunology 2022Quote: EAE was induced by injection of 200 μg of myelin oligodendrocyte glycoprotein epitope MOG35-55 (#RP10245, Genscript) emulsified in complete Freund’s adjuvant (CFA ...
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bioRxiv - Biochemistry 2024Quote: The PDCoVIL121_2014 S glycoprotein ectodomain (Genbank KJ481931.1) and SD2018/300 (Genbank KJ481931.1) were cloned into pcDNA3.1+ plasmid by GenScript with the host N-terminal signal peptide sequence ...
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bioRxiv - Evolutionary Biology 2021Quote: BtKY72 RBD construct (BtKY72 S residues 318-520) was synthesized by GenScript into a CMVR plasmid with a N-terminal mu-phosphatase signal peptide and a C-terminal hexa-histidine tag (-HHHHHHHH ...
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bioRxiv - Microbiology 2021Quote: The cDNAs encoding ACE2 orthologs (Table S1) were synthesized by GenScript and cloned into pLVX-IRES-zsGreen1 vectors (Catalog No ...
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bioRxiv - Immunology 2021Quote: ... Mice receiving oral vaccine were administered a combination of S1 (GenScript), LTB-NN ...
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bioRxiv - Immunology 2021Quote: ... Plates were coated overnight with 100 ng/well of S1 (Genscript) in coating buffer (0.015M carbonate/bicarbonate buffer ...
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bioRxiv - Microbiology 2021Quote: The cDNAs encoding ACE2 orthologs (Table S1) were synthesized by GenScript and cloned into pLVX-IRES-zsGreen1 vectors (Catalog No ...
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bioRxiv - Immunology 2022Quote: ... were coated with 100 ng/well Delta-S1 protein (GenScript, China) or 250 ng/well purified Delta-S protein in PBS and incubated at 4 °C overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... a multi-tag protein standard (Genscript) was serially diluted in 1xTBS with 0.5% Tween-20 buffer ...