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Citations for GenScript :
151 - 200 of 779 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... followed by affinity purification of sera over columns containing the immobilized peptide (Genscript). All antibodies used for IP and WB are listed in Supplementary Table 13.
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bioRxiv - Immunology 2021Quote: ... pcDNA3.1/CARD19-FLAG was obtained from GenScript (Refseq NM_026738.2). CARD19 was amplified and inserted into MSCV2.2 using Gibson cloning ...
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bioRxiv - Developmental Biology 2022Quote: ... a Flag-tagged FGF8b clone was obtained from Genscript CloneID ...
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bioRxiv - Immunology 2021Quote: The pcDNA3-mouseENPP1-FLAG plasmid was synthesized by Genscript. The Xac NPP-MBP-pMAL plasmid was a gift from Dr ...
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bioRxiv - Immunology 2021Quote: ... Flag-tag specific mouse antibody (Genscript, catalog no: A100187) and HA-tag specific rabbit antibody (Cell Signaling ...
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bioRxiv - Biochemistry 2021Quote: ... 60 µL of 300 µg/mL FLAG peptide (GenScript) was used to elute protein from the beads.
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bioRxiv - Cell Biology 2021Quote: ... or Wt or mutated ELOF1-Flag were synthesized (Genscript) and inserted in a pLenti-CMV-puro-DEST plasmid 65 ...
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bioRxiv - Immunology 2023Quote: ... 293T cells were transfected with pcDNA3.1-ELKS-Flag (Genscript), pEF1-K6a (no tag) ...
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bioRxiv - Microbiology 2023Quote: ... Full-length and C-terminal constructs were purified by Ni-IDA affinity chromatography (GenScript) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted fractions were analyzed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE; Figure S1) gels run under denaturing conditions using SurePAGE Bis-Tris gels (GenScript) and MES-SDS running buffer (GenScript ...
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bioRxiv - Cell Biology 2021Quote: ... BamHI-mCherry-FLAG-HA-MKAKU41-BamH1 was synthesized by Genscript and cloned in pUC18 at the BamHI restriction site ...
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bioRxiv - Microbiology 2022Quote: ... HA or flag-tagged nsp5,7,8,12 constructs (synthesized from Genscript. Inc.) in a 12-well plate ...
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bioRxiv - Neuroscience 2019Quote: ... sapiens thyrotropin receptor (TSHR-FLAG) (Genscript USA Inc., Clone OHu18318D), H ...
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bioRxiv - Biochemistry 2019Quote: ... and eluted with 0.5 mg/mL 3X FLAG peptide (Genscript) in base lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... DYKDDDDK (Flag)-tagged human FAM57B plasmid was purchased from Genscript. Hemagglutinin (HA)-tagged human CerS plasmids were generated as described76.
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bioRxiv - Immunology 2023Quote: ... YAP1 and Mid1 with C-terminal Flag were synthesized (GenScript) and cloned into pcDNA3.1 plasmid using In-Fusion cloning (Clontech) ...
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bioRxiv - Biochemistry 2023Quote: ... and 500 μg/mL synthesized FLAG peptide (GenScript Co., Ltd.). The eluted protein was further purified by SEC using a Superose 6 increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... C-terminally FLAG tagged constructs in pcDNA: PFD3 (GenScript, NM_003372), PFD5 (GenScript ...
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bioRxiv - Developmental Biology 2024Quote: ... 8% surePAGE gels (GenScript) were used for electrophoresis and Immobilon-FL PVDF membranes (Millipore ...
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bioRxiv - Immunology 2021Quote: ... GST-tagged NSUN2 proteins were purified by affinity chromatography using reduced glutathione resin (GenScript, L00206) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and was purified across two columns equipped with Ni2+ affinity resin (Ni-NTA Resin, GenScript) and MBP (PurKine MBP-Tag Dextrin Resin 6FF ...
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bioRxiv - Immunology 2024Quote: All constructs contained a C-terminal histidine affinity tag and were codon optimized by GenScript for mammalian cell expression ...
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bioRxiv - Molecular Biology 2019Quote: ... and pcDNA3.1 Flag-tagged MCM subunits (ORF cDNA clones from Genscript). After 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids expressing C-terminally Flag-tagged Cav2.3 were obtained from GenScript. Cultured human embryonic kidney 293 (HEK293 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pcDNA3-SHP-Flag vector for SHP was synthesized by GenScript. The primers and sequence information of all reporter constructs is provided in Supplemental table S1 (https://figshare.com/s/650749d0f09c1c5d4b2e) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plasmid pcDNA3 encoding ACE2-Flag was obtained from GenScript (OHu20260) and pcDNA3 encoding ACE2-SBP-6xHis was obtained from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2023Quote: ... and NPR3 genes from the FLAG-tagged plasmids purchased from GenScript into the appropriate vectors from the NanoBiT® PPI MCS Starter System (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Flag- tagged IRF7 and phospho-mimetic mutant were purchased from GenScript Biotech Corporation (Piscataway ...
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bioRxiv - Biochemistry 2024Quote: N-terminally biotinylated synthetic FLAG peptide variants were ordered from Genscript. These were dissolved in SPR buffer (150 mM NaCl ...
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bioRxiv - Developmental Biology 2021Quote: ... Then gel electrophoresis was conducted at 120V for 90min using page gels (Genscript, M81615C), protein were then transferred to PVDF membranes (Bio-Rad ...
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bioRxiv - Biophysics 2023Quote: ... Samples were visualized by SDS-PAGE (4-20% gradient gel, Sure Page Gels, GenScript), and band intensity was determined using Image Lab (Bio-Rad) ...
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bioRxiv - Physiology 2019Quote: ... The primary antibody was prepared using a custom affinity-purified rabbit polyclonal antibody (Genscript, Piscataway, NJ) produced against Rhodnius prolixus RhoprCAPA-2 (EGGFISFPRV-NH2 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged Ni-affinity column (2 mL Ni-NTA, Genscript) and contaminant proteins were removed with wash buffer (50 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Gradient gels (4-12% GenScript) were used in all experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-12% gel (GenScript, #M00654) and separated by MOPS buffer (GenScript ...
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bioRxiv - Biochemistry 2024Quote: ... Tris-MOPS-SDS gels (GenScript). The resolved proteins were then transferred to PVDF membranes (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... Bis-Tris Protein Gel (GenScript), and blotted ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids expressing FLAG-tagged hRpn13 or hRpn131-279 were generated commercially (GenScript) by inserting synthesized coding sequence for full length hRpn13 (NM_007002.3 ...
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bioRxiv - Cell Biology 2022Quote: ... The human FLAG-tagged EMC3 plasmid was purchased from GenScript (catalog #: OHu03021D). The human FLAG-tagged EMC5 plasmid (catalog # ...
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bioRxiv - Cell Biology 2019Quote: ... Flag-tagged TRMT6 and TRMT61A mammalian expression vectors were purchased from Genscript. Two additional Flag tags where inserted into the vector coding TRMT61A ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 spike protein (ECD, His & Flag Tag) (GenScript Z03481). Proteins were biotinylated using EZ-Link™ Sulfo-NHS-Biotin ...
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bioRxiv - Cell Biology 2020Quote: ... Bound protein was eluted using an excess of FLAG peptide (Genscript, China). The eluate was concentrated using Sartorius spin columns with a cutoff of 30kDa (Sartorius) ...
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bioRxiv - Biophysics 2020Quote: ... and the protease inhibitor cocktail plus 200 μg/ml FLAG peptide (GenScript). The eluent was then concentrated using a 50-kDa cut-off Centricon (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... the FLAG-tagged Vigilin cDNA was purchased from Genscript (Clone ID: OHu17734) and the Myc-tagged SERBP1 cDNA was purchased from Genscript (Clone ID ...
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bioRxiv - Molecular Biology 2023Quote: The following commercial antibodies were used: FLAG (A00187, GenScript, mouse, 1:1000); HA (11867423001 ...
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bioRxiv - Cancer Biology 2023Quote: ... mENPP1-WT sequence was amplified from pcDNA3-mENPP1-FLAG (synthesized by Genscript) using pLenti_mENPP1_fwd and pLenti_mENPP1_rev primers in Table S1 and inserted into the XbaI-BamHI sites of pLenti-CMV-GFP-Puro (Addgene) ...
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bioRxiv - Immunology 2023Quote: Mammalian expression vectors employed in this study include pcDNA3.1-ELKS-Flag (Genscript), pcDNA3.1-IKKε-Flag (OHu2671 ...
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bioRxiv - Microbiology 2023Quote: ... cell-based expression system and RBD proteins were purified using Protein A affinity resin (Genscript Cat#: L00210). Protein purities were assessed by SDS-PAGE ...
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KNL1 and NDC80 represent new universal markers for the detection of functional centromeres in plantsbioRxiv - Genetics 2023Quote: ... immunization of rabbits and peptide affinity purification of antisera were performed by GenScript (KNL1; Piscataway, NJ, USA) and Biomatik (NDC80 ...
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bioRxiv - Molecular Biology 2023Quote: SDS-PAGE gels were run under denaturing conditions using SurePAGE™ Bis-Tris 12% gels (GenScript) and Tris-MES-SDS Running buffer (GenScript) ...