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Citations for GenScript :
701 - 750 of 838 citations for Mouse Anti Clostridium Difficile Toxin A Antibody TA5 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and were batch bound to 1mL of anti-DYKDDDDK G1 affinity resin (Genscript) per L cells for 2hrs ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was flowed over an Anti-FLAG resin (GenScript, Piscataway, NJ, US, L00432) column at 1 mL/min ...
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bioRxiv - Molecular Biology 2020Quote: ... The first round of selection was performed with anti-FLAG affinity resin (GenScript) and captured complexes were eluted with 3xFLAG-peptide (ApexBio) ...
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bioRxiv - Immunology 2022Quote: His tag-IP was performed using anti-His affinity resin (GenScript L00439-1) and Myc tag-IP was performed using anti-Myc affinity resin ...
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bioRxiv - Cell Biology 2024Quote: ... Codon-optimized anti-cMPL scFV DNA was synthesized by GenScript (Piscataway, NJ, USA). DT390-biscFV(cMPL ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was incubated with anti-Flag affinity resin (GenScript Co., Ltd.) at 4 °C for 90 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and supernatants were incubated with anti-DYKDDDDK G1 Affinity beads (Genscript; L00432-10) or anti-GFP magnetic beads (Bio-Linkedin ...
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bioRxiv - Microbiology 2024Quote: ... (30) using anti-OROV nucleoprotein IgG (1:100 dilution; custom made by Genscript). Primary delete and no infection controls were performed to identify non-specific binding of the secondary detection antibody or all reagents ...
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bioRxiv - Immunology 2024Quote: ... anti-SARS nanobody NbS72-Biv (500 ng/ml) was preincubated with RBDmFc (Genscript) to serve as neutralization control ...
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bioRxiv - Microbiology 2024Quote: Sepharose 4B resin (SigmaAldrich, #4B200) and G1 anti-DYKDDDDK resin (GenScript, #L00432-10) were pre-washed four times with wash buffer (lysis buffer without protease inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was collected and incubated with anti-Flag G1 affinity resin (Genscript) at 4 °C for 40 min ...
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bioRxiv - Cell Biology 2024Quote: ... 2 ml of anti-His-tag affinity resin (GenScript Biotech, Piscataway, NJ, USA) was added into the concentrated culture medium and incubated at 4 °C overnight with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... and the resulting supernatants were incubated with anti-FLAG G1 Affinity Resin (GenScript) for 3 h with gentle agitation at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript, NJ, USA) which involves rapid amplification of cDNA ends ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This 338aa protein fragment was then expressed in E.coli and used to immunize two rabbits for antibody production by GenScript. ELISA titer > 1:128,000 and target protein fragment binding validation by western blot and cell line overexpression.
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bioRxiv - Immunology 2022Quote: ... 6 and 8 were analyzed with the cPass™ SARS-CoV- 2 neutralization antibody detection kit (GenScript, Cat #L00847) to detect any antibodies that neutralize the interaction between the RBDdelta and the ACE2 receptor ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used custom polyclonal antibodies raised against recombinant fragment antigens generated by rabbits’ immunization (GenScript, Piscataway Township, NJ, USA). Each recombinant fragment was injected into three rabbits ...
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bioRxiv - Microbiology 2023Quote: ... The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co ...
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bioRxiv - Microbiology 2023Quote: ... species-independent surrogate virus neutralization test (sVNT) (cPass™ SARS-CoV-2 Neutralization Antibody Detection Kit, GenScript, the Netherlands). The test was performed as prescribed by the manufacturer using a cut-off of ≥ 30 % for positivity and < 30 % for negativity ...
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bioRxiv - Microbiology 2023Quote: ... Electrophoresed material was transferred to nitrocellulose membranes and blots were developed with primary antibodies against the Strep-tag (Genscript) or RNA polymerase (RNAP ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-HRP (1:2000, GenScript, M00091), RL2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Electrophoresed material was transferred to nitrocellulose membranes and blots were developed with primary antibodies against the Strep-tag (Genscript) for detection of Strep-tagged TraD ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000; A01736-100, GenScript) and His tag (1:1,500 ...
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bioRxiv - Immunology 2024Quote: Antibodies were then purified using 1- to 2-mL of protein A or G resin (Genscript L00210 and L00209) with gravity columns (Bio-Rad 7321010) ...
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bioRxiv - Cell Biology 2024Quote: ... UK). AFF3ir-ORF2 (Cat. No. C0302HL300-4) and AFF3ir-ORF1 (Cat. No. C0302HL300) antibodies were from GenScript (NJ, USA). GAPDH (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... is a custom rabbit polyclonal antibody synthesized against the Best4 extracellular domain (290-454 aa) and purified by Genscript for the purposes of this study ...
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bioRxiv - Biochemistry 2020Quote: Secondary assessment of the RBD-ACE2 interaction blocking potential of the isolated nanobodies was performed using the Genscript SARS-CoV-2 Neutralization Antibody Detection Kit (#L00847, Genscript) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 20-minute washes with 33 ul of GluA1 peptide (20 ug/ul) representing the antibody epitope (GenScript Biotech, Piscataway, NJ). ATVs were then immediately used and continually stored on ice at 4 °C.
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bioRxiv - Plant Biology 2020Quote: ... The pellet was discarded and the supernatant employed for Co-IP analysis using polyclonal specific antibody against the whole FBN2 protein (produced by GENSCRIPT) and Dynabeads Co-Immunoprecipitation Kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The anti-Mps1 antibodies were generated in rabbits against a recombinant Mps1 protein fragment (residues 440-764) of the protein by Genscript. The company provided affinity purified antibodies that we validated by purifying kinetochores from yeast strains with Mps1 or Mps1-13Myc and confirming that the antibody recognized a protein of the correct molecular weight that migrated more slowly with the 13Myc epitope tags ...
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bioRxiv - Immunology 2022Quote: ... Matched pairs of antibody VH and Vλ/Vκ sequences were commercially cloned into plasmids containing an IgG1 or relevant light chain backbone and expressed as recombinant antibody (Genscript). mAbs were also expressed in-house by transient transfection of Expi293 cells (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody against Ft-L was made using recombinant human Ft-L subunit as antigen by GenScript (Nanjing, China).
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bioRxiv - Biochemistry 2021Quote: ... The supernatant fraction was then incubated with 5 μg of the indicated antibodies and protein A-agarose beads (GenScript L00210) at 4°C on a nutator for 5 h ...
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bioRxiv - Genetics 2022Quote: ... potentially due to their age. We additionally attempted to raise new antibodies using the previously published epitopes (Bickel et al. 2010) (synthesized by GenScript) in chickens (SMC-5 ...
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bioRxiv - Physiology 2021Quote: ... whereas the β-subunit of VHA was immunodetected using a custom-made polyclonal rabbit antibody (epitope: AREEVPGRRGFPGYC; GenScript, Piscataway, USA). These antibodies have been previously used in the inner ear of the Pacific chub mackerel (Scomber japonicus ...
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bioRxiv - Immunology 2021Quote: ... the VH and VL regions of 2H1 and 2H1-hybrid antibodies were codon-optimized using a proprietary algorithm and synthetized by GenScript and then cloned into the commercially available pFUSE vectors (InvivoGen) ...
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bioRxiv - Immunology 2020Quote: ... A second serum aliquot was tested with a surrogate virus neutralization test that detects isotype-independent RBD neutralizing antibodies (cPass, GenScript) (18) ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-peptide antibodies against CHMP7 pSer3 (3891 and 3892) were generated by immunisation of rabbits with KLH-conjugated peptides (MWpSPEREAEAPAGGC) by GenScript Biotech (Netherlands ...
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bioRxiv - Plant Biology 2023Quote: ... Colonies exhibiting VENUS fluorescence and an AphVII cassette knock-in at the CAS9 target site were examined for accumulation of the CreTPT3 protein by immunodetection using CreTPT3 antibodies generated by GenScript USA Inc (Piscataway ...
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bioRxiv - Biophysics 2023Quote: ... The anti-Scm3 antibodies were generated in rabbits against a recombinant Scm3 protein fragment (residues 1-28) of the protein by Genscript. The company provided affinity-purified antibodies that we validated by immunoprecipitating Scm3 from yeast strains with Scm3-V5 and confirming that the antibody recognized a protein of the correct molecular weight that was also recognized by α-V5 antibody (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... LY-CoV-1404/bebtelovimab antibody variable domain sequences were acquired from the structure reported in (45) and recombinant antibody was cloned and produced by Genscript. mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Custom rabbit polyclonal antibodies recognising CG2233 were generated using PolyExpressTM Premium antigen-specific affinity purified pAb package provided by GenScript. Antibodies were generated against recombinant CG2233 that lacks its N-terminal signal sequence (MFSINAVILGILVTSVMA ...
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bioRxiv - Neuroscience 2023Quote: ... while the Copiagag antibodies were generated against a Copia peptide antigen (see Figure 1) by immunizing rabbits with the peptide LMVVKNSENQLADIC (GenScript).
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bioRxiv - Synthetic Biology 2023Quote: ... Light chain of CTX/ATZ antibody and heavy chain fused with EndoTag at C-term constructs were ordered in CMVR from Genscript. Antibody-EndoTag fusions were then expressed via transient co-transfection of the EndoTag-heavy and light chains into Expi293F cells (Life Technologies ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A custom-made antibody directed against peptide CKVGEPRGVSPEDMG of the sialic acid synthase (NCBI accession # XP_021429200) was purchased (GenScript U881EER070) and used at a dilution of 1/2000 ...
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bioRxiv - Biophysics 2024Quote: ... TTR type was determined by transferring the gel contents to a 0.2 µm nitrocellulose membrane and probing with a primary antibody (1:1000) directed against the C-terminal region of the wild type TTR sequence (GenScript). Horseradish peroxidase-conjugated goat anti-rabbit IgG (Invitrogen ...
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bioRxiv - Genetics 2023Quote: An antibody against CENP-C made in guinea pig was made by generating a clone expressing amino acids 502-939 (Genscript). This guinea pig anti-CENP-C was used at 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... The recombinant monoclonal antibodies of IgG subtypes were purified after incubation of filtered Expi293F cell supernatant with Protein G resin beads (GenScript) at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Purified rGdhA was used to immunize New Zealand Rabbits and antibody titer in serum was checked after 3rd immunization (followed by a booster dose if required) by ELISA (GenScript). The rabbits were sacrificed once the antibody titer was >1:512,000 ...
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bioRxiv - Developmental Biology 2024Quote: The antibodies used in this study were as follows: A custom antibody against ExoA was generated in rabbits using purified full-length protein as the antigen (GenScript). Mouse Polyglycylated-tubulin antibody (1:1000 ...