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Citations for GenScript :
451 - 500 of 809 citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The codon-optimized sequence of His-tagged E2 (Genscript Biotech) based on an HCV variant of the genotype 1a H77 strain (Genbank ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoblotting was performed as reported (20) using anti-His (GenScript) or anti-carrot FBA (14 ...
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bioRxiv - Plant Biology 2019Quote: ... coli and affinity purified using Glutathione Resin (GenScript) and TALON® Metal Affinity Resin (Clontech) ...
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bioRxiv - Microbiology 2020Quote: ... coli expression in pET-20b(+) plasmid from GenScript. We transformed One Shot™ BL21(DE3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli and sub-cloned into pET28a(+) vectors (Genscript). Each ferredoxin was expressed in BL21(DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... coli was obtained in pET22b vector from Genscript. LceB without the secretion signal was PCR amplified off the plasmid to include restriction sites NdeI and BamHI ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using Ni-NTA chromatography (Genscript). AiEvo2 protein was complexed with guide RNA (IDT ...
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bioRxiv - Bioengineering 2022Quote: ... coli K5 and codon optimization was done (GenScript). The primers used for amplifying different gene fragments and the associated restriction sites are shown in Table 3 ...
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bioRxiv - Immunology 2020Quote: ... labeling with 1:200 biotinylated anti-Strep tag antibody (GenScript) was followed by 1:500 BrilliantViolet 421 conjugated with Streptavidin (Biolegend) ...
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bioRxiv - Molecular Biology 2019Quote: ... A 3xFLAG-2xHA tag was synthesized by GenScript (Piscataway, USA) and PCR amplified using primer pair MT-A70_HA-F/HA_R for PfMT-A70 and YTH1_HA-F/HA_R for PfYTH (Supplementary Table 9) ...
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bioRxiv - Immunology 2023Quote: ... THETM V5 Tag antibody (both at 1:5000 dilution, GenScript), and CLIPA8 primary antibody (1:5000 dilution ...
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bioRxiv - Cell Biology 2022Quote: Human His-CLASP1 (NM_015282.2) in pFastBacHT vector was purchased from Genscript. The cDNA encoding full-length human CLASP2α was purchased from Dharmacon (Accession ...
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bioRxiv - Biochemistry 2023Quote: ... His-ERK was further purified using 1 mL glutathione resin (GenScript) to remove any residual GST-tagged MKK1.
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bioRxiv - Immunology 2021Quote: The SARS-CoV2 RBD-L452R construct California-B.1.429 (L452R) was synthesized by GenScript into CMVR with an N-terminal mu-phosphatase signal peptide and a C-terminal octa-histidine tag (GHHHHHHHH ...
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bioRxiv - Microbiology 2020Quote: Mouse mAb 10G6H5 against SARS-COV2 S protein was purchased from GenScript (Piscataway, NJ). Rabbit antisera against the S1 subunit ...
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bioRxiv - Biochemistry 2020Quote: ... coli codon optimization was ordered from GenScript (Piscataway, NJ). The expression and purification of SARS-CoV-2 Mpro was described as previously.11
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bioRxiv - Neuroscience 2020Quote: ... Coli ArcticExpress and purified from inclusion bodies by GenScript. Recombinant RTN3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli or yeast and synthesized by GenScript (Piscataway, NJ) or Twist Biosciences (San Francisco ...
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bioRxiv - Cell Biology 2021Quote: ... coli lysates using glutathione-agarose or -sepharose (Sigma; GenScript) and Talon metal affinity resin (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: ... coli and synthesized (Genscript Inc, Integrated DNA Technologies Inc) with NdeI site on the 5’ end and a termination codon (TAA or TAG ...
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bioRxiv - Immunology 2022Quote: ... coli and purchased in the pUC57 vector from GenScript (GenScript ...
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bioRxiv - Cell Biology 2020Quote: ... coli optimal codons and cloned into pUC57 by GenScript USA, ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli codon optimization were ordered from GenScript (Piscataway, NJ). The Mpro gene was then subcloned into pE-SUMO vector as described previously7,8 ...
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bioRxiv - Microbiology 2021Quote: ... coli AW1.7 were also synthesized and cloned by Genscript. All synthesized sequences are presented in Table S5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... coli and synthesized in vitro by GenScript (Piscataway, NJ). The resulting gene cassettes were digested with restriction enzymes and tandemly ligated into a custom Hb expression vector (Natarajan et al. ...
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bioRxiv - Microbiology 2023Quote: ... coli KTE188 (Genbank: ANTE01000038)26 was synthesized by Genscript together with its native promoter and terminator sequences and cloned into plasmid pSG1 35 ...
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bioRxiv - Cell Biology 2021Quote: cDNA constructs with the indicated epitope tags were synthesized (Genscript, USA) and provided as entry clones ...
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bioRxiv - Biochemistry 2021Quote: ... SMT (SUMO-like tag) fusion protein in a pET28a vector (Genscript). Quick Change mutagenesis was performed to generate the W611A mutant of hP13 ZnF5-WWE1-WWE2 ...
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bioRxiv - Biochemistry 2020Quote: ... containing a TEV protease cleavage site and a His6 tag (Genscript). All constructs were verified with DNA sequencing of both strands.
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bioRxiv - Biochemistry 2023Quote: ... a rabbit anti-Strep-tag IgG antibody (Genscript, Piscataway, NJ, USA) and a goat anti-rabbit IgG Alexa 488 conjugate as secondary antibody were used ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides containing an N-terminal biotin tag were purchased from GenScript and dissolved according to manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were anti-strep tag (GenScript, A01732, 1/1000), anti-PHF21A (in house ...
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bioRxiv - Neuroscience 2020Quote: ... SARS-CoV2 Spike protein (100 nM, S1 domain aa16-685, Cat# Z03485, Genscript, Piscataway, NJ), EG00229 (Cat#6986 ...
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bioRxiv - Plant Biology 2020Quote: ... coli (Supplementary Table 4) and synthesized by GenScript (Piscataway, NJ). The Arabidopsis THI4 sequence was the native cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... coli expression (Supplementary Table 3) and synthesized in vitro (Genscript). For protein expression ...
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bioRxiv - Microbiology 2020Quote: ... coli situated in pET28b were purchased from Genscript (Piscataway, NJ). Sequence data for these constructs are provided as supplemental data ...
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bioRxiv - Biochemistry 2024Quote: ... coli expressed saposins: We purchased from Genscript (Piscataway, NJ, USA) the codon-optimized SapB and SapD (UniProt P07602 ...
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bioRxiv - Molecular Biology 2021Quote: ... The tag was removed by histidine tagged Bovine Enterokinase (GenScript Biotech Corporation) before using the proteins for DNA binding assays.
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1:200 rabbit anti-S tag (Genscript A00625) overnight and 1:1000 mouse anti GFP (Thermo Fisher A-11120 ...
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bioRxiv - Bioengineering 2021Quote: Monoclonal antibodies against GAA and GILT-tag sequence were generated by Genscript. Mouse plasma samples were screened for the presence of GAA protein using JessTM ProteinSimple and following manufacturer’s protocol (ProteinSimple ...
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bioRxiv - Microbiology 2022Quote: ... and pangolin) with a C-terminal V5 tag were synthesized by GenScript as described previously 42 ...
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bioRxiv - Synthetic Biology 2020Quote: DNA sequences encoding proteins with 6xHis tags were codon-optimized by Genscript and cloned into pET28b+ or pET29b+ vector under the control of a T7 promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... USP7 was expressed with an N-terminal myc-tag from pcDNA3.1 (Genscript). All mutations were generated by Genscript ...
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bioRxiv - Biochemistry 2021Quote: ... a mouse monoclonal DKY-Tag antibody diluted 1:1,000 (GenScript, Cat# A00187) and a mouse monoclonal anti-β-Actin antibody diluted 1:1,500 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... gene blocks containing A5 fused with alternative purification tags (Genscript; Table 1) were inserted via standard cloning techniques and digested with NcoI and NotI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... which contains an N-terminal pelB signal sequence and hexahistidine tag (Genscript). The construct was transformed into chemically competent C41 (DE3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... using an anti-Histidine-tag primary antibody (Genscript A00186; 1:3000 dilution) and an IR800 conjugated secondary antibody (Li-Cor Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... An E gene DNA standard (pUC57-2019-nCoV-PC:E, GenScript, Piscataway, NJ) was also run at the same time for conversion of Ct value to genomic copies ...
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bioRxiv - Immunology 2021Quote: ... the E subgenomic mRNA sequence was inserted into a pcDNA3.1 vector (Genscript) and transcribed using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The primary antibody for Western blot is Mouse-anti-His mAb (GenScript, Cat.No.A00186). The concentration was determined by BCA protein assay with BSA as a standard ...