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Citations for GenScript :
451 - 500 of 649 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: RBD and NP end-point titers were determined using standard ELISA and plates coated with 500 ng/mL SARS-CoV-2 Spike-protein Receptor-Binding Domain (RBD) (GenScript, Piscataway NJ) or 1ug/mL SARS-CoV-2 nucleocapsid protein (NP) ...
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bioRxiv - Immunology 2021Quote: ... resuspended at a density of 15 million/mL in complete RPMI and 100 μL of cell suspension containing 1.5 million cells was added to each well of a 96-well round-bottomed tissue culture plate and stimulated ex vivo with a peptide pool consisting of 15mer peptides overlapping by 11 amino acids spanning the S protein (GenScript, Piscataway, NJ), at a concentration of 1.2 μg/mL of each peptide in the presence of 1 μg/mL anti-CD28 ...
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bioRxiv - Immunology 2021Quote: RBD end-point titers were determined using standard ELISA and plates were coated with 500 ng/mL SARS-CoV-2 Spike-protein Receptor-Binding Domain (RBD) (GenScript, Piscataway NJ) Heat inactivated plasma (1:50 in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... flanking the EVT region (intron is between the N and G residues) and the KLH-conjugated antibody was purified by protein G column (GenScript USA Inc.). Samples were mounted in VECTASHIELD (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... A DNA fragment for a floxed transcription stop transcription site (3 copies of SV40 late poly A sequence) followed by a H2B protein fused to mPlum was synthesized by Genscript (Piscataway, NJ) and inserted into pUC57-Kanamycin plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... and then subjected to denaturation at 100 °C for 10 min after the addition of 4X protein loading buffer (GenScript Biotech, China). Then ...
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bioRxiv - Immunology 2022Quote: 15-mer peptides overlapping by 10 amino acids spanning the entire protein sequence of SARS-CoV-2 Spike were synthesized (GenScript; see Table S1). To stimulate whole blood or PBMC ...
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bioRxiv - Cell Biology 2020Quote: The endotoxin level of FHL-1 recombinant protein preparations was measured using the Toxin Sensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript, NJ, USA), according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... the recombinant N protein was constructed by inserting the N gene of SARS-CoV-2 into the pGEX-6P vector (GenScript Japan, Tokyo, Japan). Next ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2-specific T cell responses in rhesus macaque PBMCs were assessed by an IFN-γ ELISPOT assay using recombinant SARS-CoV-2 S1 protein (GenScript, Nanjing, China). Ninety-six-well plates (Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids used to express the linked-BchL-proteins carrying glycine linkers of various lengths were synthesized as codon-optimized genes (Genscript Inc., Piscataway, NJ). The longest iteration of the linked-BchL-protein was generated as described in the supplemental section.
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bioRxiv - Microbiology 2020Quote: ... glutamicum open reading frames were amplified from genomic DNA by PCR and cloned in pET-28a vector by restriction free cloning 39, while expression constructs for the other proteins (CmE2p, MtE2p, MtE2b) were provided by Genscript (Leiden, the Netherlands). In all cases ...
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bioRxiv - Bioengineering 2024Quote: Genes encoding the designed protein sequence were synthesized and cloned into pET-24a(+) E.coli plasmid expression vectors (Genscript, C-terminal 6X His tag). Plasmids were then transformed into chemically competent BL21(DE3 ...
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bioRxiv - Biophysics 2022Quote: ... was linked to cell-penetrating peptides (CPP): HK2p-CPP(R9) and HK2p-CPP (TAT) for cell penetration and synthesized by Genscript, Inc Control peptide ScrP-CPP was synthesized by Peptide2.0 ...
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bioRxiv - Immunology 2021Quote: ... One million cells per well were added to a U-bottom 96-well plate and were stimulated with 5 μg/ml of pools of overlapping SARS-CoV-2 S protein peptides (GenScript USA Inc, Piscataway, NJ). The stimulation was performed by incubation for 6 h at 37°C and 5% CO2 in the presence of Protein Transport Inhibitor Cocktail (brefeldin A ...
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bioRxiv - Biochemistry 2023Quote: ... The gene encoding for the GAF2 domain from All1280 of Nostoc PCC 7120 (NCBI protein ID BAB73237.1, UniProtKB Q8YXD3, amino acids 562-727) was ordered from Genscript (codon optimized for E. coli) in the pUC18 cloning vector ...
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bioRxiv - Biochemistry 2020Quote: ... Cell supernatant from hybridoma clones and subclones (GenScript) was used 1:10 ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of cell lysate was saved as an input control and the remaining cell lysate was incubated with Glutathione MagBeads (GenScript, L00327) overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... PLA2G7 and SIAE from Chinese hamster ovary (CHO) cells were expressed in CHO-3E7 cells and purified (>90% purity) by GenScript (Piscataway, NJ).
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently transfected with a custom plasmid (Genscript) expressing hsTRPA1 and dTom fluorescent reporter as for screening experiments ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with mouse anti-HA antibody (Genscript) diluted 1:500 in PBS supplemented with 0.5% goat serum and 0.01% Tween-20 (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... cells and purified using the His6–affinity resin (GenScript). These proteins were labeled at the single Cys site (C459 ...
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bioRxiv - Cancer Biology 2020Quote: MC38 cells were transfected with the idAdpgkG plasmid (Genscript) using the piggyBAC vector/transposase system with Lipofectamine 2000 (Thermo ...
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bioRxiv - Immunology 2022Quote: ... cells were given 100 ng/ml SIITFEKL peptide (Genscript) for 3 hours.
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bioRxiv - Cancer Biology 2021Quote: ... the melanoma cells were spiked with gp100+ antigens (GenScript) just before addition of T-cells ...
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bioRxiv - Immunology 2023Quote: ... 293T cells were transfected with pcDNA3.1-ELKS-Flag (Genscript), pEF1-K6a (no tag) ...
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bioRxiv - Microbiology 2021Quote: ... A549 cells were transduced with codon-optimized human ACE2 (Genscript) cloned into pBABE-puro [54] (Addgene) ...
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bioRxiv - Immunology 2019Quote: ... 1 × 106 cells were incubated with influenza Gp33 peptide (Genscript) at a final concentration of 05μg/mL in the presence of fluorescence-conjugated antibodies against CD107a and CD107b together with Brefeldin A for 5h before additional flow cytometric staining.
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bioRxiv - Bioengineering 2019Quote: ... Cell adhesive peptides (GRGDSPC, or scrambled GRDGSPC; >95% purity; GenScript) were dissolved in PBS containing 10 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK 293T cells were transfected with pLentiCRISPR V2 vectors (GenScript) expressing S ...
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bioRxiv - Cancer Biology 2023Quote: Cell lysates were separated on an SDS-polyacrylamide gel (Genscript) and transferred to a nitrocellulose membrane using a wet transfer system (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... OCRL1-/-(OCRL KO) HK2 and HEK293T cells were prepared by GenScript Inc ...
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bioRxiv - Biochemistry 2021Quote: RBD inhibition assay: HEK-293T cells stably expressing hACE2 (GenScript M00770) were seeded into a 24-well plate at an initial density of 6 x 104 cells per well ...
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bioRxiv - Microbiology 2019Quote: ... cstK coding sequence was also optimized for mammalian cell expression (GenScript), amplified by PCR and cloned into pDXA-3C (46 ...
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bioRxiv - Biochemistry 2019Quote: ... Surface expression was measured by incubating cells with 200uL TMB (Genscript) per well and reaction was stopped by transferring 100uL of developed colored solution to a 96-well plate already containing 100uL of 1M H2SO4 ...
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bioRxiv - Biochemistry 2020Quote: ... 1+/c-(k) - dyk expression vector for mammalian cells by GenScript Corporation (Piscataway ...
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bioRxiv - Genetics 2020Quote: ... cells were stimulated with increasing concentrations of agonist GIP (RP10795, GenScript) for 45 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... were reacted with equimolar cell-degradable peptide containing sequence KCGPQGIWGQCK (GenScript), 3.0 mM CRGDS basement membrane-mimicking peptide (GenScript) ...
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bioRxiv - Immunology 2023Quote: ... Cells were stimulated with 2.5 μM TSKB20 (ANYKFTLV) peptide (Genscript Inc.) or 50 ng/mL PMA plus 1 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... were codon-optimized for human cell expression (GenScript Codon Optimization Tool). Gene fragments were each cloned into a pαH eukaryotic expression vector with a C-terminal HRV 3C protease cleavage site ...
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bioRxiv - Biochemistry 2024Quote: ... plasmids used for mammalian cell over-expression were purchased from GenScript. NLuc-WT-CCNE1-3xFLAG ...
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bioRxiv - Cell Biology 2019Quote: ... cells were pretreated with interleukin 4 (IL-4) (Cat. # Z02925-10, GenScript) at a concentration of 5 ng/ml for 24h ...
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bioRxiv - Cell Biology 2019Quote: ... cells were incubated with 1:200 rabbit anti-S tag (Genscript A00625) overnight and 1:1000 mouse anti GFP (Thermo Fisher A-11120 ...
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bioRxiv - Systems Biology 2019Quote: ... half of the cells were stimulated with erythropoietin (2 ng/mL; GenScript) 24 hours after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T stable transfected with ACE2 (GenScript M00770, 8000 cells per assay), Caco2 cells (40,000 cells per assay) ...
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bioRxiv - Cancer Biology 2021Quote: Peptides for spectra validation and T cell stimulation were synthesized by GenScript with >98% purity and TFA removal.
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bioRxiv - Immunology 2020Quote: ... cells were cultured in the presence of LCMV GP33-41 peptide (GenScript) and Protein Transport Inhibitor Cocktail (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... variable genes were optimized for human cell expression and synthesized by GenScript. VH and VL were inserted separately into plasmids (gWiz or pcDNA3.4 ...
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bioRxiv - Microbiology 2020Quote: ... variable genes were optimized for human cell expression and synthesized by GenScript. VH and VL were inserted separately into plasmids (gWiz or pcDNA3.4 ...
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bioRxiv - Systems Biology 2023Quote: ... B16 cells were pulsed for 0.5h with 5μg/ml OVA257-264 (Genscript), and then washed twice in RPMI ...