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Citations for Singer Instruments :
1 - 50 of 77 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... The working plate (96-well plate) was used as a source plate for robotic spotting using a ROTOR HDA benchtop robot (Singer Instruments, United Kingdom) on rectangular OmniTray plates (Singer Instruments ...
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bioRxiv - Genetics 2023Quote: ... plates containing 1536 colonies were pinned onto YES plates using a RoToR arraying robot (Singer Instruments) and incubated for 24 hours at 32°C ...
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bioRxiv - Microbiology 2021Quote: ... on rectangular OmnyTray plates (Singer Instruments) with solidified SB medium as the target plate ...
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bioRxiv - Microbiology 2024Quote: ... The plates were replicated onto agar YPD and solidified wort plates using a ROTOR HDA (Singer Instruments, UK), sealed and incubated at 20 °C for 210 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were moved from agar plates into liquid 384-well polystyrene growth plates using the ROTOR HDA (Singer Instruments). Liquid cultures were grown overnight in 50 μl of SD medium in a shaking incubator (LiCONiC Instruments ...
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bioRxiv - Cell Biology 2022Quote: Cells were moved from agar plates into liquid 384-well plates using the RoToR bench-top colony arrayer (Singer Instruments). Liquid cultures were grown overnight in synthetic medium with 2% glucose (SD ...
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bioRxiv - Cell Biology 2022Quote: Cells were moved from agar plates into liquid 384-well plates using a RoToR bench-top colony arrayer (Singer Instruments). Liquid cultures were grown overnight in low biotin 0.512nM d-biotin (sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... on rectangular OmniTray plates (Singer Instruments, United Kingdom) with solidified medium on the target plate as recently described8 ...
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bioRxiv - Genetics 2020Quote: ... deletion library was arrayed on solid agar EMM media plates in in 384 colony/plate format using a RoToR HDA robot (Singer Instruments). After two days of growth plates were scanned and raw colony sizes extracted using gitter R package [54] ...
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bioRxiv - Microbiology 2022Quote: ... colonies were pinned on the antibiotic-containing plates and once on a vehicle control plate at 1536 colony density using the ROTOR HDA (Singer Instruments). This experiment was done in duplicate using two different source plates ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The liquid cultures were briefly resuspended by pipetting and arrayed in a 384-position configuration on CM plates (2% w/v agar, 50 mL/plate) using a ROTOR HDA (Singer Instruments). These plates were grown for 2 days at 30°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Digested cells were plated onto a YPD plate and tetrads were dissected to individual spores arrayed on the plate using a SporePlay+ tetrad dissection microscope (Singer Instruments). Mating type of isolated haploids was determined by PCR as previously described (Huxley et al. ...
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bioRxiv - Microbiology 2023Quote: ... An ordered PA14 transposon mutant library was pinned from source plates in 1536 colony density onto the rectangular agar plates using a ROTOR HDA robotic colony replicator (Singer Instruments). The screen was performed in duplicates using different source plates ...
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bioRxiv - Microbiology 2021Quote: ... The working plate was used as a source plate for robotic spotting using a ROTOR HDA benchtop robot (Singer Instruments, United Kingdom) on rectangular OmnyTray plates (Singer Instruments ...
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bioRxiv - Systems Biology 2019Quote: ... and phenotyping plates using the Phenobooth (Singer Instruments, Somerset, UK) imaging platform and software ...
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bioRxiv - Evolutionary Biology 2019Quote: Using a Singer ROTOR robotic plate handler (Singer Instrument Co. Ltd), overexpression and reference strains where transferred from glycerol archives to agar plates and then robotically combined into 1536-density agar plates (SC-URA+GLU+G418 ...
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bioRxiv - Genetics 2021Quote: ... Plates were visualized on a dissection microscope (MSM System 400, Singer Instruments) immediately at 3 hrs and at 24 hrs ...
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bioRxiv - Genetics 2022Quote: ... The images of these plates were recorded using a Phenobooth (Singer Instruments) for subsequent SGA analyses.
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bioRxiv - Systems Biology 2022Quote: ... Plates were replica plated with an automated RoToR HDA system (Singer Instruments).
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bioRxiv - Plant Biology 2023Quote: ... the tetrads were re-arrayed into a rectangular agar plate (Singer Instruments) in a 96-array format and replicated using TAP plates or TAP + paromomycin plates.
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bioRxiv - Genetics 2024Quote: The plates were imaged with the PhenoBooth Colony Counter (Singer Instruments, UK) after 72 hours of incubation and the size of the individual colonies was used as a proxy for fitness ...
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bioRxiv - Cell Biology 2019Quote: ... Synthetic sick and lethal phenotypes were identified using PhenoBooth plate imager (Singer Instruments) and PhenoSuite colony counting and analysis software (Singer Instruments) ...
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bioRxiv - Cell Biology 2024Quote: ... or YPO agar plates using a Singer pinning robot (Singer Instruments and Control). The plates were incubated at 26 ...
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bioRxiv - Genomics 2019Quote: ... Ten individual cells were micromanipulated on YPD plates with the MSM400 micromanipulator (Singer Instruments) and grown at 30°C for 2 days ...
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bioRxiv - Molecular Biology 2022Quote: Using the benchtop RoToR HDA robotic plate handler (Singer Instruments Co Ltd, Roadwater, UK) strains were transferred from glycerol stocks to solid rich medium plates (YPDA ...
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bioRxiv - Synthetic Biology 2019Quote: Plates were imaged over two-hour time courses using a Phenobooth (Singer Instruments, Somerset, UK) imaging platform ...
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bioRxiv - Synthetic Biology 2023Quote: ... plates were imaged in white light with bottom lighting in a Phenobooth Imager (Singer instruments). Both raw colony size and fluorescence were extracted from plate pictures with the functions of the Pyphe Python package (Kamrad et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Solid media was cast in polystyrene plates from a single batch (Singer Instruments, SBS-format PlusPlates). Each plate was cast on top of the same perfectly level surface with precisely 50 mL of synthetic grape must medium at 50 C ...
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bioRxiv - Genetics 2022Quote: ... in a density of 1536 colonies per plate using the RoToR HDA system (Singer Instruments, UK). Each single deletion strain was plated in quadruplicates allowing replicates for consistent SGA analysis ...
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bioRxiv - Genetics 2020Quote: Tetrad dissection was performed on YPD plates using the MSM 400 dissection microscope (Singer Instrument Company) and isolated spores were incubated three days at 25°or two days at 30° ...
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bioRxiv - Microbiology 2021Quote: ... 50 mL of the medium were used for each plate (SBS-format PlusPlates, Singer Instruments, United Kingdom) and supplemented with black food dye (30 μL/plate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast populations were subsampled and transferred to fresh plates by robotic pinning (ROTOR HDA, Singer Instruments, UK).
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bioRxiv - Synthetic Biology 2022Quote: ... 10-fold dilution series were prepared in 96-well microtiter plates and the Rotor HDA+ (Singer Instruments) was used to perform 7x7 grit pinning on the described solid media ...
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bioRxiv - Cell Biology 2022Quote: ... a defined numbers of spores were dissected on YES plates using a Singer MSM micromanipulator (Singer Instruments). Between 50 and 100 spores per biological replicate were dissected ...
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bioRxiv - Genetics 2021Quote: ... high-resolution images of phenotypic plates were taken using Phenobooth after 3 days of incubation (Singer Instruments, UK). The colony sizes were calculated in pixels using Phenosuite software (Singer Instruments ...
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bioRxiv - Genomics 2020Quote: ... 24 tetrads were dissected on fresh YPD plates with a SporePlay™ dissection microscope (Singer Instruments, Somerset, UK). After 5 days of incubation at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Yeast strains were assembled in 1536-colony format on agar plates using a pinning robot (Rotor, Singer Instruments). For each tFT strain ...
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bioRxiv - Cell Biology 2020Quote: ... The bait and prey colonies were mated on YPD plates using a RoToR HDA-Robot (Singer Instruments Co. Ltd) and incubated overnight at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... Library was spotted on plates in 1536-colony format using a ROTOR colony pinning robot (Singer Instruments, Somerset, UK). Each library strain had four technical replicates spotted next to each other (2×2) ...
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bioRxiv - Genomics 2019Quote: ... glucose 20 g.l−1) matrix plate to a 1,536 density format using the replicating ROTOR™ robot (Singer Instruments). Two replicates of each parental strain were present on every plate and six replicates were present for each hybrid ...
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bioRxiv - Genetics 2020Quote: ... and then streaked onto a YPD plate for tetrad dissection using the MSM400 dissection microscope (Singer Instrument Company Ltd). Spores were grown on YPD plates at 30°C for two days ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Treated cells were then placed on a level YPD plate to perform tetrad dissection with the SporePlay (Singer Instrument). The dissection plates were incubated at room temperature for 5 days to make sure we didn’t underestimate growth ...
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bioRxiv - Genetics 2021Quote: ... Strains were pinned onto a solid YPD matrix plate to a 1,536-density format using the replicating ROTOR robot (Singer Instruments) and grown overnight at 30°C ...
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Efficient breeding of industrial brewing yeast strains using CRISPR/Cas9-aided mating-type switchingbioRxiv - Microbiology 2021Quote: ... adjacent to each other on a YPD agar plate with the aid of a MSM400 dissection microscope (Singer Instruments, UK). Plates were incubated at 25 °C for up to 5 days ...
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bioRxiv - Molecular Biology 2020Quote: ... and allowed to recover on YPD plates before being assayed for RLS which was performed using a micromanipulator (Singer instruments) to remove daughters from mother cells ...
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bioRxiv - Molecular Biology 2019Quote: ... Mating was performed on 1536-colony format YPD plates using a RoToR bench top colony arrayer (Singer Instruments; Somerset, UK). Resulting diploids were selected for deletion/DAmP libraries and ER-GFP markers KanR and URA3 ...
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bioRxiv - Cell Biology 2020Quote: ... The cultures were then diluted into fresh medium by pinning to a new 96-well plate using a RoToR pinning robot (Singer Instruments) and incubated at 23°C for 20-24 h to 1×106-8×106 cells/ml ...
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bioRxiv - Cell Biology 2020Quote: ... The cultures were then diluted into fresh medium by pinning to a new 96-well plate using a RoToR pinning robot (Singer Instruments) and incubated at 23°C for 20-24 h to 1×106-8×106 cells/ml ...
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bioRxiv - Genomics 2019Quote: ... strains were grown overnight on liquid YPD medium then pinned onto a solid SC medium with a 1,536 colony per plate density format using robot assisted pinning with ROTOR™ (Singer Instrument) and incubated overnight at 30°C ...
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bioRxiv - Systems Biology 2020Quote: ... The 2 1536-colony arrays were assembled by thawing copies of the 2 × 16 96-well plates and using a Rotor pinning robot (Singer Instruments) to spot the plates ...