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Citations for Singer Instruments :
51 - 85 of 85 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... in a density of 1536 colonies per plate using the RoToR HDA system (Singer Instruments, UK). Each single deletion strain was plated in quadruplicates allowing replicates for consistent SGA analysis ...
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bioRxiv - Genetics 2020Quote: Tetrad dissection was performed on YPD plates using the MSM 400 dissection microscope (Singer Instrument Company) and isolated spores were incubated three days at 25°or two days at 30° ...
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bioRxiv - Microbiology 2021Quote: ... 50 mL of the medium were used for each plate (SBS-format PlusPlates, Singer Instruments, United Kingdom) and supplemented with black food dye (30 μL/plate ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast populations were subsampled and transferred to fresh plates by robotic pinning (ROTOR HDA, Singer Instruments, UK).
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bioRxiv - Synthetic Biology 2022Quote: ... 10-fold dilution series were prepared in 96-well microtiter plates and the Rotor HDA+ (Singer Instruments) was used to perform 7x7 grit pinning on the described solid media ...
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bioRxiv - Cell Biology 2022Quote: ... a defined numbers of spores were dissected on YES plates using a Singer MSM micromanipulator (Singer Instruments). Between 50 and 100 spores per biological replicate were dissected ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to tetrad analyses on YEPD plates using a Singer MSM400 micromanipulator (Singer Instruments, Somerset, UK). Plates were incubated for 3-5 days at 30°C as required for the specific mutant combinations ...
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bioRxiv - Genetics 2021Quote: ... high-resolution images of phenotypic plates were taken using Phenobooth after 3 days of incubation (Singer Instruments, UK). The colony sizes were calculated in pixels using Phenosuite software (Singer Instruments ...
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bioRxiv - Genomics 2020Quote: ... 24 tetrads were dissected on fresh YPD plates with a SporePlay™ dissection microscope (Singer Instruments, Somerset, UK). After 5 days of incubation at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The bait and prey colonies were mated on YPD plates using a RoToR HDA-Robot (Singer Instruments Co. Ltd) and incubated overnight at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... Library was spotted on plates in 1536-colony format using a ROTOR colony pinning robot (Singer Instruments, Somerset, UK). Each library strain had four technical replicates spotted next to each other (2×2) ...
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bioRxiv - Genetics 2020Quote: ... and then streaked onto a YPD plate for tetrad dissection using the MSM400 dissection microscope (Singer Instrument Company Ltd). Spores were grown on YPD plates at 30°C for two days ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Treated cells were then placed on a level YPD plate to perform tetrad dissection with the SporePlay (Singer Instrument). The dissection plates were incubated at room temperature for 5 days to make sure we didn’t underestimate growth ...
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bioRxiv - Genetics 2021Quote: ... Strains were pinned onto a solid YPD matrix plate to a 1,536-density format using the replicating ROTOR robot (Singer Instruments) and grown overnight at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and allowed to recover on YPD plates before being assayed for RLS which was performed using a micromanipulator (Singer instruments) to remove daughters from mother cells ...
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bioRxiv - Cell Biology 2024Quote: All yeast manipulations were performed in high-density format (384–1,536 strains per plate) using a RoToR bench-top colony array instrument (Singer Instruments). In order to find key proteins involved in the formation of the peroxisome-peroxisome or peroxisome-lipid droplet contact sites (Figure 5A) ...
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bioRxiv - Cell Biology 2020Quote: ... The cultures were then diluted into fresh medium by pinning to a new 96-well plate using a RoToR pinning robot (Singer Instruments) and incubated at 23°C for 20-24 h to 1×106-8×106 cells/ml ...
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bioRxiv - Cell Biology 2020Quote: ... The cultures were then diluted into fresh medium by pinning to a new 96-well plate using a RoToR pinning robot (Singer Instruments) and incubated at 23°C for 20-24 h to 1×106-8×106 cells/ml ...
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bioRxiv - Systems Biology 2020Quote: ... The 2 1536-colony arrays were assembled by thawing copies of the 2 × 16 96-well plates and using a Rotor pinning robot (Singer Instruments) to spot the plates ...
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bioRxiv - Systems Biology 2020Quote: ... PCA strains were cherry-picked from the Yeast-Interactome Collection (Dharmacon, YSC5849) and mated on YPD plates using a ROTOR HDA (SINGER instruments). A negative control diploid strain that lacks DHFR was generated by mating a MATa (HO::NatMX ...
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bioRxiv - Genomics 2022Quote: ... MA lines were pre-cultured in YPD broth and pinned onto a solid YPD plate to a 1536 density format using the replicating ROTOR robot (Singer Instruments). The plates were incubated for 24 hours at 30°C and were scanned with a resolution of 600 dpi at 16-bit grayscale ...
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bioRxiv - Genetics 2020Quote: ... cells were plated to YPD at 23°C at which time individual colonies were cherry-picked into 96-well plates to allow for automated pinning using the Singer ROTOR (Singer Instruments). Next ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight in yeast extract peptone dextrose (YPD) in 96-well plates and inoculated onto solid YPD and spider media using a 96-pin replicator tool (Singer Instruments). Plates were then incubated at the indicated temperature and oxygen conditions for 7-9 days ...
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bioRxiv - Synthetic Biology 2024Quote: ... colony counts were obtained to determine total library size and plates were imaged for fluorescence using GFP imaging conditions on a Phenobooth+ (Singer Instruments) system (excitation LED ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated at 30 °C for 48 h and 72 h and photographed individually using a PhenoBooth+ (Singer Instrument, USA) apparatus ...
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bioRxiv - Systems Biology 2024Quote: The AID-v2 library was assembled in 1536-colony format on SC(MSG)-Leu + hygromycin/clonNAT plates using a pinning robot (Rotor, Singer Instruments), with four technical replicates per strain placed next to each other in a 2×2 group ...
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bioRxiv - Systems Biology 2024Quote: The AID-v1 library was assembled in 1536-colony format on SC(MSG)-Leu+Ade Raf/Gal + hygromycin plates using a pinning robot (Rotor, Singer Instruments). For each ORF ...
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bioRxiv - Microbiology 2024Quote: ... Precultures were grown overnight and diluted to an OD600 nm of 1 (WT) or 0.1 (deletion backgrounds) and used to inoculate 384 well plates using a Singer Rotor+ replicator (Singer Instruments, UK), resulting in further ∼1:600 dilution and starting OD of ∼0.003 and ∼0.0003 ...
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bioRxiv - Cell Biology 2024Quote: ... Mating was performed on SPA solid medium plates and the spores generated in the crosses were dissected using a dissection microscope (MSM 400; Singer Instruments) on YES agar plates.
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bioRxiv - Cell Biology 2021Quote: ... query and library strains were handled in high-density format (384-1536 strains per plate) using a RoToR bench-top colony arraying instrument (Singer Instruments, UK). In short ...
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bioRxiv - Genetics 2022Quote: ... The library was first organised in 96 and 384 density formats and subsequently used as a source to generate the final working copy of two Plus Plates (Singer Instruments, UK) in a density of 1536 colonies per plate using the RoToR HDA system (Singer Instruments ...
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bioRxiv - Cancer Biology 2022Quote: ... and pinned in quadruplicate to achieve a 384-colony density (i.e. 4 technical replicates for each position of the microtiter plate) using a Singer RoToR robot (Singer Instruments, Inc. Somerset UK). Strains were grown on YES solid agar media with the indicated camptothecin concentrations ...
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bioRxiv - Biochemistry 2023Quote: ... A small amount of cells was streaked out on YPD plates and spores were segregated using a Singer MSM400 micromanipulator (Singer Instruments, Somerset, UK). The plates were then incubated for 3 days at 30°C.
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bioRxiv - Synthetic Biology 2024Quote: ... 380 single colonies were picked onto 384-well microtiter plates with LB containing kanamycin utilizing the PIXL colony picking robot (Singer Instruments, Somerset, UK). Microtiter plates were incubated overnight on an Multitron HT (Infors AG ...
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bioRxiv - Cell Biology 2022Quote: ... Transformations were performed as described in [173] and were done with the aid of the Benchtop RoToR HAD robotic plate handler (Singer Instruments Co. Ltd, Roadwater, UK) and the Multidrop Combi (ThermoFisher Scientific ...