Labshake search
Citations for Takara Bio :
401 - 450 of 6767 citations for ssc mir 323 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... cDNA was prepared from total RNA using the PrimeScript RT-PCR kit (Takara, #RR037Q). Relative gene expression of the cDNA was assayed using a 7500 Fast real-time PCR instrument (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
-
bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
-
bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was reverse transcribed using the Perfect real-time cDNA reverse transcription kit (TaKaRa). Quantitative PCR was performed with 1 μg of cDNA per sample per target gene using AceQ qPCR SYBR Green master mix (Vazyme ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from total RNA using a PrimeScript Perfect real-time reagent kit (Takara Bio) according to the instructions provided by the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... Reverse transcription of RNA was performed using PrimeScriptTM reagent Kit (Perfect Real Time) (Takara Bio Inc.) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reactions were performed with gene-specific forward and reverse primers using the PrimeScript RT-PCR kit (TaKaRa) on the StepOne Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Developmental Biology 2019Quote: ... and the Thermal Cycler Dice Real Time System (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... or Thermal Cycler Dice Real Time System III (Takara) using β-actin as an internal control ...
-
bioRxiv - Biochemistry 2022Quote: ... and the Thermal Cycler Dice Real Time System (Takara). The primer sequences for real-time PCR are listed in Table EV1.
-
bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). All primers used in this study are listed in Table S5 (No ...
-
bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). Three biologically independent experiments were performed and used for the analysis.
-
bioRxiv - Molecular Biology 2023Quote: ... on Thermal Cycler Dice Real Time System II (TaKaRa). The relative mitochondrial DNA content normalized by nuclear-genome content was determined using the ΔΔCt method.
-
bioRxiv - Molecular Biology 2024Quote: ... and the Thermal Cycler Dice Real-Time System (Takara).
-
bioRxiv - Plant Biology 2023Quote: ... and inserted into the Aor51HI-linearized pMpGWB123/223/323/423 vectors using In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan).
-
bioRxiv - Cell Biology 2020Quote: ... nested PCR was performed using PCR Mycoplasma Detection Set (Takara, 6601).
-
bioRxiv - Microbiology 2023Quote: ... or Omicron variant (G339D) using a SARS- CoV-2 Direct Detection RT-qPCR Kit (TaKaRa Bio Inc., Shiga, Japan) with each specific primer/probe ...
-
bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR detection was performed on each group of samples by using a reverse transcription kit (Takara Bio, Japan) and fluorescence quantitative kit (Takara Bio ...
-
bioRxiv - Cell Biology 2020Quote: ... Real-time PCR was performed on 15 ng of cDNA using SYBR Premix Ex Taq (Tli RNaseH Plus) (Takara RR420W) in a reaction volume of 20 μl ...
-
bioRxiv - Cancer Biology 2019Quote: ... The resultant cDNA was subjected to real-time PCR by using SYBR® Premix Ex Taq™ II (Takara Bio) and specific primer sets (Takara Bio) ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed with the ABI PRISM Fast 7500 Real-Time PCR engine using the TB Green Premix Ex TaqII (TIi RNaseH Plus) (TaKaRa) and the miRcute Plus miRNA qPCR kit (SYBR Green ...
-
bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCR was performed as previously described using TB Green Premix Ex Taq II (Takara Bio Inc, Japan) and a QuantStudio 7 Real-Time PCR system (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Quantitative real-time RCR (qRT-PCR) was carried-out using SYBR Premix Ex Taq™ (Tli RNaseH Plus) (TaKaRa, Japan) and CFX96TM real-time PCR system (Biorad ...
-
bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
-
bioRxiv - Genomics 2020Quote: ... and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus) (Clontech,USA). The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen ...
-
bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The results were analyzed based on standard curves from a serial dilution of cDNA ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative expression of genes was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using SYBR Green Supermix (TaKaRa); β-actin served as a housekeeping gene ...
-
bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using TOYOBO THUNDERBIRD SYBR qPCR Mix on a Thermal Cycler Dice Real Time System (Takara Bio). The average threshold cycle value (CT ...
-
bioRxiv - Genomics 2022Quote: Genomic DNA was used as a template for quantitative PCR analysis on a Thermal Cycler Dice Real Time System (TaKaRa) using KAPA SYBR Fast qPCR Kit (KAPA Biosystems ...
-
bioRxiv - Plant Biology 2023Quote: ... Synthesized cDNA was amplified by real-time quantitative PCR (qPCR) with TB Green Premix Ex Taq (Tli RNaseH Plus) (Takara) using the QuantStudio 3 system (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genes were analysed by quantitative real-time PCR in triplicate with at least three independent biological replicates using SYBR premix EX Taq (Takara). Quantification was performed as indicated in the figure legends ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA copies of IBVs were quantified by real-time PCR using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa) and the following primer pair ...
-
bioRxiv - Pathology 2023Quote: ... Expression levels of the genes were validated by quantitative real-time PCR analysis with SYBR Green (Takara Bio, CA, USA). Cycling parameters were 95°C for 20 seconds ...
-
bioRxiv - Molecular Biology 2024Quote: ... The qPCR reactions were carried out and the signals were detected using a real time PCR system (catalog no. TP950, TaKaRa). For RT-qPCR ...
-
bioRxiv - Molecular Biology 2024Quote: ... qRT–PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The data were normalized using 18S rRNA as a reference ...
-
bioRxiv - Microbiology 2023Quote: High-throughput qPCR was performed by an outside firm (Resistomap, Finland) using the qPCR SmartChip Real-Time PCR cycler (Takara). The qPCR cycling conditions and initial data processing were carried out as previously described by Wang et al ...
-
bioRxiv - Developmental Biology 2019Quote: ... Complementary DNA was synthesized with 1μgtotal RNA using a PrimeScript™ RT-PCR Kit (Takara). The real-time PCR with the mixture reagent SYBR Green (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1μg of RNA via the One Step RT-PCR Kit (TaKaRa). Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... Amplification was performed using a One Step PrimeScript RT-PCR Kit (Takara Bio, Otsu, Japan), and the following real-time PCR conditions were applied ...
-
bioRxiv - Neuroscience 2022Quote: ... For first-strand cDNA synthesis the Takara PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan) was used with 50 μM Oligo (dT ...
-
bioRxiv - Genomics 2020Quote: ... The cDNA first strand synthesis was done using a PrimeScript RT-PCR Kit (Clontech, USA) and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... total RNA was reverse transcribed to cDNA using PrimeScript RT-PCR Kit (Takara, Dalian, China). All primers used in this study were recorded in Table 1 ...
-
bioRxiv - Cancer Biology 2019Quote: ... The RNA were reverse transcribed using the Advantage® RT for PCR kit (Applied Clontech) and gene expression was analyzed by RT-qPCR with the LighCycler® 480 Real-Time PCR system ...
-
bioRxiv - Cell Biology 2021Quote: ... 1.0μg of total RNA was used to prepare cDNA using Titanium RT-PCR kit (TaKaRa). The real-time PCR was performed on the cDNA using mouse TERT and FST primers as described previously (27 ...
-
bioRxiv - Microbiology 2022Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
-
bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was performed with the PrimeScript High Fidelity RT-PCR Kit (Takara, Cat. R022A). RT-PCR was run on a 7900HT Fast RT-PCR instrument (Applied Biosystems ...
-
bioRxiv - Genetics 2023Quote: ... according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara). We used 2-ΔΔc to indicate the relative gene expression level of each sample.
-
bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and each RNA concentration was measured by quantitative PCR after reverse transcription using PrimeScript One Step RT-PCR Kit (TaKaRa) with each specific primer (Table S2).