Labshake search
Citations for Takara Bio :
301 - 350 of 3750 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... 500-1000ng of total RNA was reverse transcribed using the Advantage RT-for-PCR Kit (Takara) and qPCR performed in quadruplet using SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... Quantitative real-time RT–PCR was carried out using the SYBR premix Ex Taq kit (TaKaRa). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR was performed with SYBR® Premix Ex Taq™ (Takara Bio, Shiga, Japan) in a 7300 Real-Time PCR system (Applied Biosystems ...
-
bioRxiv - Microbiology 2022Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on Applied Biosystems QuantStudio ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Samples were analyzed using a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan) on an Applied Biosystems QuantStudio ...
-
bioRxiv - Developmental Biology 2021Quote: ... PrimeScript RT-PCR and SYBR Premix ExTaq Kits were from Takara (Cat#2641A, Cat#RR420, Japan). BCA protein assay and ECL kits were from Beyotime Institute of Biotechnology (Shanghai ...
-
bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized with 100 ng RNA using Advantage RT-PCR Kit (Clontech, Mountain View, USA). Reaction condition for real time PCR was 95°C for 3min (1 cycle) ...
-
bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit ...
-
bioRxiv - Biochemistry 2021Quote: ... qRT-PCR analysis was performed using cDNA synthesized by the Prime Script RT Reagent Kit (TaKaRa). Each qRT-PCR was conducted in a 20-μL reaction mixture containing 2 μL of diluted template cDNA ...
-
bioRxiv - Genetics 2022Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).
-
bioRxiv - Cell Biology 2023Quote: ... Lentiviral titration was performed by RT-qPCR using the Lenti-X qRT-PCR Titration Kit (Takara) and the concentration of lentivirus used for this paper was found to be above 1 × 107 copies / mL ...
-
bioRxiv - Genetics 2023Quote: ... Reverse transcription and quantitative real-time PCR were performed with PrimeScriptTM RT Master Mix (Takara, RR037A) and TB Green Premix Ex TaqTM II (Takara ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time qPCR amplification was performed using the PrimeScript™ RT-PCR Kit (Takara, Catalog#RR420). Alp ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA from the total RNA was synthesized using the Advantage® RT-for-PCR kit (Takara) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (RR096A, Takara Bio Inc.), and primer pairs for Zcchc3 (5′-CTCTCTATGCCTTCTTAAACCGA-3′ and 5′-CATCTGCACGCTACAGTTCT-3′ ...
-
bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with a Takara Thermal Cycler Dice® Touch (Takara Bio, Kusatsu, Japan). The RT-PCR program was as follows ...
-
bioRxiv - Microbiology 2023Quote: ... Reverse transcription and amplification were performed using One Step PrimeScript™ RT-PCR Kit (Takara, Japan) and reaction performed and visualized using Stratagene Mx3000P qPCR System real-time PCR system (Agilent ...
-
bioRxiv - Neuroscience 2024Quote: ... RT–PCR was performed using a TB Green TM Premix Ex Taq Kit (Cat# RR820A, Takara) on a Light Cycler Real-Time PCR System (480II ...
-
bioRxiv - Cell Biology 2024Quote: ... RT-PCR was performed with the SYBR Premix Ex TaqTM kit (DRR041A, Takara Bio, Shiga, Japan) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... primer design was done using primer design tool (Takara). For inducible expression of the gene ...
-
bioRxiv - Plant Biology 2020Quote: ... Gene specific primers were used to amplify cDNA by PCR using Ex Taq Hot Start Version (Takara). The thermal cycling program consisted of initial 4-minute denaturation at 95°C followed by 30 to 40 cycles of 30 seconds at 95°C ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The PCR products were sequenced by primer walking and Sanger sequencing with Takara LA Taq (Takara, Japan), a dGTP BigDye Terminator Cycle Sequencing FS Ready Reaction Kit (Thermo) ...
-
bioRxiv - Biophysics 2019Quote: ... Excess primers were removed from the PCR products using NucleoSpin silica-membrane columns (Clontech, Mountain View, CA) and the products were further purified in 3% agarose-TBE gels and subsequently reisolated using gel-purification kits (Clontech).
-
bioRxiv - Physiology 2022Quote: ... Relative quantitative PCR was conducted with a TB Green Primer Ex Taq II (Takara Bio Inc., Japan) using LightCycler® 96 Real-Time PCR System (Roche ...
-
bioRxiv - Plant Biology 2023Quote: ... Primers flanking the region targeted for replacement were designed and used for Terra Direct genotyping PCR (Clontech) to identify transformants lacking the wild-type fragment ...
-
bioRxiv - Genomics 2024Quote: ... except that an alternative oligo-dT primer from the SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.), which also included a random 10mer sequence as a unique molecule identifier (UMI ...
-
bioRxiv - Plant Biology 2020Quote: ... Then the first-strand cDNA synthesis was primed with an oligo (dT) primer by using a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated by standard procedure from 20-30 heads of homozygous Aprt5 flies and reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto, Japan). At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was generated using a template□switch anchored reverse□transcription polymerase chain reaction (RT□PCR) based on the SMARTer pico cDNA PCR Synthesis Kit (Clontech, Mountain View, CA, USA). The final cDNA product was then subjected to a clean□up step using PCR Clean DX Beads (Aline Biosciences ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNAs bound by miR-2861 were purified with Trizol reagent (Takara, Shiga, Japan), and assessed through qRT-PCR.
-
bioRxiv - Immunology 2021Quote: ... using One Step TB Green(tm) PrimeScript(tm) RT-PCR Kit II (SYBR Green) (RR086B, TaKaRa, JAPAN). Relative copy number was determined by calculating the fold-change difference in the gene of interest relative to GAPTH ...
-
bioRxiv - Molecular Biology 2020Quote: ... Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara; Kusatsu, Shiga, Japan) and used as templates for quantitative PCR ...
-
bioRxiv - Genetics 2021Quote: ... and One Step TB Green™ PrimeScript™ RT-PCR Kit II (SYBR Green) (TaKaRa RR086B, Japan).
-
bioRxiv - Physiology 2021Quote: ... 1 μg RNA was used to synthesize first strand of cDNA using PrimeScriptTM RT-PCR Kit (TaKaRa). qPCR was conducted using PrimeScriptTMRT Master Mix (TaKaRa ...
-
bioRxiv - Cancer Biology 2020Quote: ... and used for first-strand cDNA synthesis using the First-Strand Synthesis System for RT-PCR (Takara). Each sample was processed in triplicate ...
-
bioRxiv - Plant Biology 2022Quote: ... One microgram DNAse-treated RNA was converted to cDNA using Prime RT-PCR kit (Takara Bio Inc.). The qRT-PCR was performed on 11 selected DEGs containing DRE elements ...
-
bioRxiv - Plant Biology 2021Quote: ... The cDNA used for RT-PCR was synthesized using the PrimeScrip First-Strand cDNA Synthesis Kit (TaKaRa). RT-PCR cycling conditions were as follows ...
-
bioRxiv - Microbiology 2021Quote: ... Each RNA sample was reverse transcribed to 50 μl cDNA with RT-PCR Prime Script Kit (Takara). The cDNA (5 μl ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... qRT-PCR assays were carried out using the SYBR PrimeScript™ RT reagent Kit (TaKaRa, Kusatsu, Japan) following manufacturer‟s instructions and ABI 7500 Software v2.0.6 (Applied Biosystems ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... SARS-COV-2 virus detection was performed using the One Step PrimeScript RT-PCR kit (TaKaRa, Japan) on the LightCycler 480 Real-Time PCR system (Roche ...
-
bioRxiv - Neuroscience 2020Quote: ... RNA was extracted and cDNA was synthesized by PCR (PrimeScript RT reagent kit with gDNA Eraser, TaKaRa). SaCas9 was amplified by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2023Quote: ... For quantitative RT-PCR 1ug of total RNA was treated with 5 Units of DNase I (Takara) and cDNA synthesized with SuperScriptIII (Life Technologies ...
-
bioRxiv - Immunology 2023Quote: Quantitative RT-PCR was performed with a SYBR Premix Ex Taq II (Tli RNaseH Plus) (RR820; Takara) on a 7500 Real-Time PCR system (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the PimeScript RT-PCR kit (TAKARA) and analyzed by qPCR on LightCycler96 system (Roche ...
-
bioRxiv - Microbiology 2023Quote: Amplification and detection were performed using the One Step PrimeScript™ RT-PCR Kit (RR064A, Takara Bio) with the following primers targeting the IAV M segment ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
-
bioRxiv - Microbiology 2023Quote: ... using the Takara One Step TB Green PrimeScript™ RT-PCR kit (Takara Bio Inc., Shiga, JP) and the Roche LightCycler® 96 real-time PCR instrument (Roche Applied Science ...
-
bioRxiv - Cancer Biology 2023Quote: ... RT-PCR was performed using diluted cDNA (1:5 in water) and PrimeStar DNA Polymerase (Takara, R010A) with primers and PCR conditions listed in Table 1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Primers were designed using the primer design tool from Takara Bio (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools) ...
-
bioRxiv - Cell Biology 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate using SYBR Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa), Fast SYBR Green Master Mix (Invitrogen ...