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Citations for Takara Bio :
551 - 600 of 6767 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The cDNAs used for RT-PCR were synthesized using PrimeScript First-Strand cDNA Synthesis Kits (Takara, Osaka, Japan). RT-PCR cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... and one μg RNA was converted to cDNA by using the PrimeScript™ RT-PCR Kit (Takara, Japan) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT-PCR Kit (Takara). Three technical replicates were measured for gene expression levels in each cDNA sample ...
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bioRxiv - Cell Biology 2021Quote: RNA was quantified (by Nanodrop) and retrotranscribed to cDNA (PrimeScript™ RT-PCR Kit - Takara Bio Cat. # RR014A). Real time PCR was performed with KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Neuroscience 2019Quote: ... Single stranded cDNA from the total RNA was synthesized with a RT-PCR kit (Clontech, Mountain View, CA) according to the kit’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and 1 µg was used for cDNA synthesis with the PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). Of the cDNA ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated using a template□switch anchored reverse□transcription polymerase chain reaction (RT□PCR) based on the SMARTer pico cDNA PCR Synthesis Kit (Clontech, Mountain View, CA, USA). The final cDNA product was then subjected to a clean□up step using PCR Clean DX Beads (Aline Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... and monitored with the Thermal Cycler Dice Real Time System II (TaKaRa). The relative gene expression was normalized to the expression of OsACT ...
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bioRxiv - Microbiology 2021Quote: ... was used with a Thermal Cycler Dice Real Time System TP800 (TaKaRa) for RT-qPCR ...
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bioRxiv - Biochemistry 2019Quote: ... using Thermal Cycler Dice® Real Time System Single (TaKaRa, Shiga, Japan) to determinate the amount of mitochondrial DNA (mtDNA ...
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bioRxiv - Cell Biology 2021Quote: ... and analysed on real-time qPCR using SYBR® Premix ExTaqII (Takara) on an AriaMx Realtime PCR system (Agilent Genomics ...
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bioRxiv - Cell Biology 2021Quote: ... and a Thermal Cycler Dice Real Time System TP800 (Takara Bio Inc.). The results were normalized to the Rpl26 expression ...
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bioRxiv - Genetics 2022Quote: ... using gene specific primers and Thermal Cycler Dice Real Time System (TAKARA). The sequences of gene specific primer set were shown as follows ...
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bioRxiv - Molecular Biology 2022Quote: ... and Thermal Cycler Dice Real Time System (Model TP800; TaKaRa Bio Inc). The sequences of the primers for qRT-PCR are shown in Supplemental Table 1 ...
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bioRxiv - Plant Biology 2024Quote: ... and 10 μ L SYBR premium ExTaq Perfect Real Time (TaKaRa Bio), adjusted to a final volume of 20 μL with water ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with PrimeSTAR GXL DNA polymerase (Takara) using gene-specific primers (Table S7) ...
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bioRxiv - Cell Biology 2021Quote: RT-PCR was conducted using PrimeSTAR Master Mix (Takara, Japan) according to manufacturer’s instructions along with PCR control ...
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bioRxiv - Microbiology 2021Quote: ... 10 μL of 2× One Step RT-PCR buffer (TaKaRa), and 5 μL ddH2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative RT-PCR was performed with SYBR Green regent (Takara) on StepOne Plus Real-Time PCR system (Applied Biosystem) ...
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bioRxiv - Microbiology 2021Quote: ... The extracellular SARS-CoV-2 RNA in the culture supernatant was analyzed using SARS-CoV-2 direct detection RT-qPCR kit (RC300A; TaKaRa-Bio, Shiga, Japan). The infectivity of SARS-CoV-2 in the culture supernatants was determined at 48 h post-infection ...
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bioRxiv - Molecular Biology 2019Quote: ... miRNA was reverse transcribed using a Mir-X miRNA First-Strand Synthesis Kit (Clontech, USA) and the following procedure ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using the TB GreenTM Premix Ex TaqTM II (Takara Bio Inc., Kusatsu, Japan) according to the manufacturer’s protocol for a total reaction volume of 20 μl ...
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bioRxiv - Microbiology 2021Quote: ... Real time PCR (qPCR) was carried out in triplicates for each sample using TB Green® Premix Ex Taq™ II (TaKaRa, Dalian, China) in The CFX96 touch Real-Time PCR System (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... The real-time PCR reaction system is as the followings: 10 µL of SYBR Premix Ex Taq™ (Takara Biom edical Technology, Beijing, China), 0.5 µM of each forward or reverses primer and 100-500 ng of DNA template ...
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bioRxiv - Pathology 2022Quote: Reverse transcription PCR (RT-PCR) was performed with 500 ng RNA per reaction by TaKaRa PrimeScriptTM RT Master Mix (Perfect Real Time ...
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bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Plant Biology 2022Quote: ... and then used for RT reactions using the PrimeScript RT Reagent Kit (Takara), with an equimolar mix of random hexamers and polyA primers ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed using the PrimeScript RT reagent kit (Takara) in conjugation with custom stem-loop primers specific to siRNA guide strands ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from plant samples with Purelink (Nacalai Tesque, Japan) and reverse transcribed with PrimeScript Reagent Kit Perfect Real Time (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted with a Bacterial RNA Extraction Kit and was immediately reverse-transcribed into cDNA using a PrimeScriptT™ RT-PCR Kit (TAKARA, Daliang, China). The common primer pair GSPilACF/GSPilACR was used to test genomic DNA contamination ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR and cDNA synthesis were performed using SMARTer Ultra Low Input RNA Kit v3 following manufacturer’s protocol (Clontech). RNA isolated from 1000 cells was used as starting material ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-PCR and cDNA synthesis were performed using SMARTer Ultra Low Input RNA Kit v3 following manufacturer’s protocol (Clontech). Single cells were used directly as starting material without RNA extraction to avoid extra loss of RNA ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using a PrimeScript II High Fidelity One Step RT-PCR Kit (R026A, Takara Bio, Shiga, Japan), purified using a QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2022Quote: ... Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa, #RR820A) using LightCycler 96 (Roche ...
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bioRxiv - Immunology 2020Quote: ... The RT products were amplified by nested PCR following the PrimeSTAR® HS DNA Polymerase kit protocol (Takara, Japan), with primers for TCRα and TCRβ ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... CDC-USA assay targeting gene N (IDT # 10006713) and One Step PrimeScript™ III RT-PCR Kit (TaKaRa #RR600A). Serial dilutions of reference material were prepared ranging from 1 to ~10M genome equivalents per reaction ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The measurement was performed after diluting the droplets 100-fold with 1 mM EDTA (pH 8.0) and using One Step TB Green PrimeScript PLUS RT-PCR Kit (Takara).
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bioRxiv - Immunology 2019Quote: LDH Cytotoxicity Detection kit (MK401, Takara Bio) was used as per manufacturer instructions to detect cell killing.
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bioRxiv - Plant Biology 2020Quote: ... Takara Prime Script™ RT kits (Takara Bio Inc. ...
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bioRxiv - Immunology 2021Quote: ... The PrimeScript RT Master Mix Kit (Takara) was used for cDNA generation ...
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bioRxiv - Cell Biology 2022Quote: ... The RT kit was obtained from Takara Bio USA (Moutain view ...
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bioRxiv - Pathology 2020Quote: ... The RT kit was obtained from Takara Bio USA (Mountain View ...
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bioRxiv - Immunology 2021Quote: ... and PrimeScript RT Reagent Kit (Takara, RR037A). Forward and reverse primers were designed to target SRBD (Forward ...
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bioRxiv - Neuroscience 2020Quote: ... and PrimeScript RT reagent kit (RR037, Takara), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... PrimeScript™ RT reagent kit (TaKaRa, RR036A) is used to generate cDNA from RNA extracted ...
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bioRxiv - Immunology 2023Quote: ... or PrimeScript RT reagent Kit (Takara, RR037A) and TB Green Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Pathology 2021Quote: ... Mycoplasma contamination was confirmed by PCR using the TaKaRa PCR Mycoplasma Detection Set (Takara, Shiga, Japan).
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bioRxiv - Molecular Biology 2021Quote: ... qPCR analyses were performed using the Thermal Cycler Dice Real Time System (Takara) or C1000 touch Thermal Cycler and CFX Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: ... and quantitative primers (Perfect Real Time Primer, Takara, Shiga, Japan, Supplemental Table S1). The expression was normalized to GAPDH within each sample.
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative real-time was carried out using TB Green Premix Ex TaqII (TaKaRa) in CFX96 real-time PCR system (Bio-Rad) ...