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Citations for Takara Bio :
301 - 350 of 6767 citations for rno mir 101a Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Quantitative real time polymerase chain reaction (qRT-PCR) was performed using SYBR Premix ExTaq (Takara, Japan) in a TP800 Real-Time PCR machine (Takara ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time polymerase chain reaction (PCR) was performed using TB Green Premix Taq II (TaKaRa) and a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... according to the instructions of SYBR®Green Real-time PCR Master Mix (Takara, Dalian, China). Reaction conditions were 95°C for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Real-time PCR analysis was performed using the Probe qPCR Mix (Takara Bio, Otsu, Shiga, Japan) and Applied Biosystems 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: The threshold cycle (Ct) values were determined by the SmartChip Real-Time PCR software (Takara Bio). Standard curves were generated by plotting the Ct values vs ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
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bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
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bioRxiv - Neuroscience 2024Quote: ... The titer of AAV was determined with a real-time PCR thermal cycler (Dice, Takara Bio). The resultant AAV1-Syn-mCherry (2.8 × 1012 GC/ml) ...
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bioRxiv - Microbiology 2024Quote: ... Plasma viral RNA copy numbers were determined via SYBR green-based real-time quantitative PCR (Takara), using SIV gag-specific primers (table supplement 4) ...
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bioRxiv - Plant Biology 2021Quote: ... After DNA digestion with Perfect Real Time gDNA Eraser Kit (TaKaRa, Otsu, Japan), first strand cDNA was synthesized using PrimeScript™ RT reagent Kit (TaKaRa) ...
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bioRxiv - Cancer Biology 2019Quote: ... Amplification and detection of mRNA were conducted by using the Thermal Cycler Dice® Real Time System (Takara Bio, TP800), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... PrimeScript® RT Master Mix Perfect Real-Time and DRR820ASYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... were reverse transcribed into cDNA in a volume of 10 µl using the PrimeScript RT Master Mix (Perfect Real Time, TaKaRa) (37°C ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was generated from each sample employing the Prime Script RT Master Mix (Perfect Real Time) (Takara Bio Inc., Japan). Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR was performed as described (Pujol et al., 2008b) by using SYBR™ Green PCR Master Mix (TaKaRa). Values were normalized to those of act-1 and were analyzed by the cycling threshold method using the appropriate qRT-PCR primers (Supplementary Table S2) ...
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bioRxiv - Molecular Biology 2022Quote: ... by RT-PCR (TaKaRa, RR014) and cloned by Gibson assembly into the vector pK27SUMO (expressing a 14His-tagged version of the yeast SUMO protein Smt3 ...
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bioRxiv - Microbiology 2021Quote: ... Comparative RT-PCR (Takara, Japan) was performed using SYBR Green Supermix via an RT-PCR machine (RocheLightCycler480 ...
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bioRxiv - Biochemistry 2023Quote: ... by RT-PCR (Takara, RR014) and cloned by Gibson assembly into the vector pK27SUMO ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was performed with the One Step TB Green PrimeScript RT-PCR Kit II (TaKaRa), and SYBR Green was used as a fluorescent dye for detection ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed on three biological replicates using an ABI StepOne PCR detection system with SYBR Green (Takara). GmUbiquitin (SUBI-2.2 ...
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bioRxiv - Genomics 2019Quote: ... Reverse transcription was performed with a PrimeScript RT-PCR Kit (TaKaRa, Japan). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase was performed using the PrimeScript™ RT-PCR Kit (Takara). mRNA relative levels of SIGLEC1 were measured by two-step quantitative RT-PCR and normalized to GAPDH mRNA expression using the DDCt method ...
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bioRxiv - Genomics 2019Quote: ... The q-PCR was performed using a PrimeScript RT Reagent Kit (TaKaRa) in a 20-μL reaction volume with 10 μL of 2× SYBR Premix Ex Taq II (TaKaRa) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... by using One Step TB Green PrimeScript PLUS RT-PCR Kit (Takara) and host RNA-specific primers (Supplementary Table S3) ...
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bioRxiv - Microbiology 2020Quote: ... using the SYBR® PrimeScript™ RT-PCR Kit (Takara, Dalian, China).
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bioRxiv - Microbiology 2020Quote: ... per the manufacturer’s instructions for PrimeScript™ miRNA RT-PCR Kit (Takara). The PCR mixture contained 3 μl cDNA ...
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bioRxiv - Cell Biology 2021Quote: ... Q-PCR was carried out using the SYRB Premix Ex Taq™ Perfect Real-Time system (Takara). The expression levels were normalized to that of the housekeeping gene GADPH ...
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bioRxiv - Immunology 2020Quote: Quantitative real-time PCR was conducted to quantify the mRNA levels using Premix Ex Taq (Takara, Japan). Total RNA was extracted from mud crab hemocytes ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed using SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara) and complementary DNA was synthesized with a PrimeScript™ RT reagent Kit with gDNA Eraser (Takara) ...
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bioRxiv - Pathology 2022Quote: ... Quantitative real-time PCR was performed by TB Green Fast qPCR Mix (Takara Bio Inc. Kusatsu, Japan) on a PCR System (Roche ...
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bioRxiv - Microbiology 2022Quote: ... All PCR reactions were performed on a Thermal Cycler Dice Real Time System III (Takara Bio, Inc.). Previously sequenced E ...
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bioRxiv - Cancer Biology 2022Quote: ... Then real-time quantitative PCR was performed using TB Green® Premix Ex Taq™ II (Takara) on the Real-Time PCR Thermal Cycler qTOWER3 (Analytik Jena) ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time quantitative PCR was performed with TB Green® Premix Ex Taq™ II (TAKARA, Japan) using a Bio-Rad CFX 96 real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... and quantified by real-time PCR with TB Green Premix Ex Taq (TaKaRa Biomedical Technology Co., Ltd.) and ABI 7500 (Applied Biosystems Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... and then the isolated RNA (1 μg) was reverse transcribed into cDNA (20 μL) using PrimeScript™ RT Master Mix (Perfect Real Time, Takara). The qPCR reaction was initiated at 95 °C for 3 min ...
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized from purified total RNA using PrimeScript RT Master Mix (Perfect Real Time; Takara Bio, Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on an ABI Prism 7500 Fast Real-Time PCR System using the SYBR Premix Ex Taq kit (Takara Bio Inc., Shiga, Japan) according to the instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: The water-in-oil droplets after the incubation step were diluted 10000-fold with 1 mM EDTA (pH 8.0) and subjected to RT-qPCR (PrimeScript One Step RT-PCR Kit (TaKaRa)) with primer 1 and 2 after heating at 95 °C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Viral load was measured by RT-qPCR using One-Step SYBR® Primescript(tm) RT-PCR kit II (Takara). CT values from serum samples were used to calculate serum viral load according to regression equation built by a set of standard viral RNA extracted from dilutions of known titre virus preparation ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR was performed using a One Step TB Green PrimeScript RT–PCR Kit II (Takara, #RR086A) on a LightCycler 2.0 instrument (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR mix (15 ul) contained One step TB Green RT-PCR kit (RR096A, Takara Bio Inc.), each primer ...
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bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 500 ng of total RNA and PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Tokyo, Japan). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Genomics 2020Quote: ... qPCR was performed using One Step SYBR PrimeScript RT-PCR kit (Takara Bio) on a 7900HT real-time system (Applied Biosystems).
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bioRxiv - Immunology 2021Quote: ... utilizing the PrimeScript™ One-Step RT-PCR kit (Takara Bio, Shiga, Japan). PCR products were then separated and visualized by agarose gel electrophoresis ...
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bioRxiv - Genomics 2019Quote: ... Total RNA was reverse transcribed using a PrimeScript RT-PCR kit (TaKaRa Bio), and qRT-PCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2019Quote: ... RT-PCR was accomplished using the SYBR premix Ex Taq kit (Takara, China) on an ABI StepOnePlus Real-Time PCR System (Thermofisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... 12.5μl RNA was used for cDNA synthesis with Advantage RT-PCR kit (Clontech). Detection of pull-down product was performed with qPCR using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... and cDNA was synthesized using the one-step PrimeScript RT-PCR Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Then the purified RNA was reversed transcribed using RT-PCR Kit (TaKaRa, Japan) with an oligo dT-adaptor primer in accordance with the manufacturer’s instructions ...