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Citations for Takara Bio :
251 - 300 of 3750 citations for hsa mir 635 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... This region was PCR amplified with DinoSL and KbrSRP-U6R1 primer set (sequences and Tm in Table 2) using the high fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Shiga Prefecture, Japan) at 94°C for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... Mir-X™ miRNA First Strand Synthesis Kit was from Takara Co. ...
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bioRxiv - Cancer Biology 2024Quote: ... The Mir-X miRNA First-Strand Synthesis Kit (TAKARA, Shiga, Japan) was used to convert RNAs into complementary DNA (cDNA) ...
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bioRxiv - Pathology 2019Quote: ... The first-strand cDNA was synthesized from 1μg total RNA using an oligo dT primer according to the protocol of PrimeScript™RT reagent Kit with gDNA Eraser (TAKARA). The expression level of PDS of cucumber ...
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bioRxiv - Genomics 2020Quote: ... The cDNA synthesis was carried out by using 5 g of total RNA or 1 g of PAPed RNA with RT primer (5-TTTTTTTTUUUTTTTTVN-3) by PrimeScript II Reverse Transcriptase (TaKaRa Bio). The full-length cDNAs were selected by Cap Trapper method 60 ...
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bioRxiv - Plant Biology 2023Quote: ... were converted to cDNA with oligo dT primers according to the instructions of the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed in accordance with manufacturer’s instructions for THUNDERBIRD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... and quantified by RT‒qPCR using Primer/Probe N2 (NIHON GENE RESEARCH LABORATORIES, INC. Miyagi, Japan) and One Step PrimeScript™ III RT‒qPCR Mix (TaKaRa) with CFX96 (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... total RNAs were reverse transcribed to complementary DNA (cDNA) using both oligo dT and random primers with PrimeScript RT Reagent Kit (Takara, #RR037A) according to manufacturer’s instructions in the presence of a synthetic external non-homologous poly(A ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was prepared from the extracted RNAs with oligo (dT) and random primers using the PrimeScript RT reagent kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μL of treated RNA was reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto Japan). Then ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative RT-PCR was carried out using SYBR Premix ExTaq™ (Takara Biotechnology, Dalian, China). The primers used in this study were listed in Supplementary Table 3.
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bioRxiv - Cell Biology 2020Quote: ... Quantitative RT-PCR was performed by using SYBR Premix Ex Taq (Takara Bio, Dalian, China) and the following primers ...
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bioRxiv - Developmental Biology 2019Quote: ... Complementary DNA was synthesized with 1μgtotal RNA using a PrimeScript™ RT-PCR Kit (Takara). The real-time PCR with the mixture reagent SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was synthesized from 1μg of RNA via the One Step RT-PCR Kit (TaKaRa). Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time quantitative RT-PCR was performed with TB Green Premix Ex Taq™ (TakaRa) using a 7500 real-time PCR system (Applied Biosystems Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed using a One Step PrimeScript RT-PCR Kit (Takara Bio, Otsu, Japan), and the following real-time PCR conditions were applied ...
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bioRxiv - Plant Biology 2021Quote: ... Youren RT-PCR products were cloned into the pENTR™/D-TOPO™ vector (Takara) and sequenced by Sanger sequencing (Applied Biosystems 3500).
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bioRxiv - Neuroscience 2022Quote: ... For first-strand cDNA synthesis the Takara PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan) was used with 50 μM Oligo (dT ...
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bioRxiv - Genomics 2020Quote: ... The cDNA first strand synthesis was done using a PrimeScript RT-PCR Kit (Clontech, USA) and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... total RNA was reverse transcribed to cDNA using PrimeScript RT-PCR Kit (Takara, Dalian, China). All primers used in this study were recorded in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA were reverse transcribed using the Advantage® RT for PCR kit (Applied Clontech) and gene expression was analyzed by RT-qPCR with the LighCycler® 480 Real-Time PCR system ...
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bioRxiv - Cell Biology 2021Quote: ... 1.0μg of total RNA was used to prepare cDNA using Titanium RT-PCR kit (TaKaRa). The real-time PCR was performed on the cDNA using mouse TERT and FST primers as described previously (27 ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was performed with the PrimeScript High Fidelity RT-PCR Kit (Takara, Cat. R022A). RT-PCR was run on a 7900HT Fast RT-PCR instrument (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... RT–PCR and agarose gel electrophoresis were performed after RNA extraction with TRIzol (Takara, China). The primers are listed in the Supplementary Table 4.
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bioRxiv - Genetics 2023Quote: ... according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara). We used 2-ΔΔc to indicate the relative gene expression level of each sample.
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bioRxiv - Neuroscience 2023Quote: ... Quantitative RT-PCR was performed using Premix Ex Taq II (Takara Bio Inc., Shiga, Japan) on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... HpCTCF cDNA was amplified by RT-PCR with TaKaRa LA-taq (TaKaRa Bio Inc., Japan) using the following primers (5’-GTTCGCCATTCATTTGAGTGGATATTTC-3’ and 5’- CTGATCAGGTAAACTCTAAATCAGATTCC- 3’).
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bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Bioengineering 2023Quote: ... Quantitative RT-PCR was performed using TB Green Premix Ex Taq II (Takara Bio Inc.) in an Applied biosystems Quantstudio 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... which then subjected to PCR using Taq Master Mix (Vazyme, p112-01) for RT-PCR or TB Green Premix EX Taq Ⅱ (Takara, RR820A) for quanti tative PCR ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Plant Biology 2023Quote: ... The expression of marker genes was detected by qRT-PCR using the One-step TB Green PrimeScriptTM RT-PCR kit II (Takara, Osaka, Japan). All genes were normalized against the level of an actin reference gene (Foo et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of ATG14 transcripts was verified by Reverse Transcription PCR (RT-PCR) on cDNA synthesized from total RNA extracted from the cell samples using Terra™ PCR Direct Polymerase Mix (TAKARA, 639270). Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel) ...
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bioRxiv - Neuroscience 2021Quote: ... Mutagenesis of pMU2-Camk2b was conducted by inverse PCR with Mighty Cloning Reagent Set (Blunt End) (Takara Bio, Japan) following to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... Cells were originally obtained from the ATCC and routinely tested negative for mycoplasma contamination (PCR Mycoplasma Detection Set, Takara).
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bioRxiv - Microbiology 2021Quote: ... specific primers were designed and PCR reactions were performed using SYBR Green Premix Ex Taq (Takara), according to the manufacturer’s protocol and the primers listed in Table S4 ...
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bioRxiv - Molecular Biology 2022Quote: ... EBIV M/L segment-specific sequences were amplified by PCR including Universal Primer A Mix (Takara), Gene-Specific Primers (GSPs ...
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bioRxiv - Evolutionary Biology 2021Quote: Amplified with V4 16S rRNA primers as above using EmeraldAmp GT PCR Master Mix (TAKARA BIO).
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was amplified with V3d(T)24 and R2SP primers and Terra PCR Direct Polymerase (Clontech) for 16 cycles ...
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bioRxiv - Immunology 2024Quote: ... Real-Time PCR was performed by using specific primers and SYBR Green ROX Mix (RR420A, Takara). β-actin was used as the internal quantitative control.
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bioRxiv - Neuroscience 2023Quote: ... AAV was titrated using quantitative PCR (qPCR) with primers for the ITR sequence (TaKaRa Bio Inc).
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bioRxiv - Genetics 2019Quote: ... A single primer (primer IIA from the Clontech SMARTer kit 5’ AAG CAG TGG TAT CAA CGC AGA GTA C 3’ ...
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bioRxiv - Microbiology 2022Quote: ... A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RT Reagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 µL reaction ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA and 200 ng of a random primer were used in reverse transcription (RT) through the cDNA synthesis kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse-transcribed into cDNA using random hexamer primers and the PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time; Takara, Dalian, China). For the target genes ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNAs were subjected to one-step real-time PCR using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.).
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription for quantitative PCR (qPCR) analyses was performed with PrimeScript RT Master Mix kit (Takara). qPCR was performed with TransStart Top Green qPCR SuperMix kit (TransGen).
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time RT-PCR analysis was performed using TB Green Premix Ex Taq (Clontech, RR420A) and following primers ...