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Citations for Takara Bio :
701 - 750 of 6767 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using PrimeScript RT kit (Takara RR037A) with 100-500 ng RNA as input ...
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bioRxiv - Biochemistry 2022Quote: ... total RNA (1.9 mg) was used to synthesize first-strand cDNA using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR reactions were performed using a Thermal Cycler Dice Real-Time System TP800 (Takara Bio), and the relative amounts of each segment were calculated.
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bioRxiv - Genetics 2020Quote: ... the plate was applied to a Thermal Cycler Dice® Real Time System II (TP900; TAKARA) with the following amplification conditions ...
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bioRxiv - Neuroscience 2021Quote: ... 72°C for 15 s performed using a Takara Thermal Dice Real-time system III (Takara) or a QuantStudio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence threshold data (Ct) were analyzed using Thermal Cycler Dice Real-Time System Software (Takara, Japan) and then exported to Microsoft Excel for further analysis ...
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bioRxiv - Molecular Biology 2021Quote: Primers were designed using the Takara Bio Perfect Real Time Support System (Takara Bio, Table 2). Primers were diluted to 50 µM in ddH20 and stored at -20°C ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time qPCR was performed (in duplicates) using the SYBR-master mix (Takara, Otsu, Shiga, Japan) and the ABI 7500 Real-time PCR system (Applied Biosystem ...
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bioRxiv - Systems Biology 2022Quote: Following the transfer of samples into a 384-well plate containing RT-PCR buffer with 3’ SMART-Seq CDS Primer IIA (SMART-Seq® v4 PLUS Kit, TaKaRa, cat# R400753); the samples were immediately denatured at 72ºC for 3 min and chilled on ice for at least 2 min ...
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bioRxiv - Neuroscience 2022Quote: ... mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc., Shiga, Japan). Real-time RT-PCR was then performed using the KAPA SYBR FAST qPCR Master Mix (2x ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was then carried out using total RNA and oligo-dT primers to synthesize the first strand cDNA using the PrimeScript One-Step RT-PCR Kit (Ver.2, Takara Biotechnology, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min and then harvested for quantification of INF α mRNA using the One-step SYBR Prime script RT-PCR kit (TaKaRa, Dalian, China) according to the manufacturer’s protocol.Briefly the total RNA was isolated from TNF-α-treated with Trizol reagent (Takara Bio ...
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bioRxiv - Genomics 2022Quote: ... Extracted RNA from each juvenile (as described in Methods S1) was converted to cDNA using PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan). The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed by targeting the SARS-CoV-2 RdRp gene as follows: each 20 µl sample consisted of 12.5 µl One Step PrimeScript™ III RT-PCR Kit (Takara Bio, Shiga, Japan), 0.5 µM Sars-CoV-2 CRV forward primer (5’-TCACCTAATTTAGCATGGCCTCT-3’) ...
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bioRxiv - Cell Biology 2020Quote: ... Evo M-MLV RT Kit with gDNA Clean for qPCR (Accurate Biotech, Changsha, Hunan, China) and Mir-X™ miRNA First-Strand Synthesis Kit (Takara, Dalian, Liaoning, China) were applied for the transcription of RNA into complementary DNA (cDNA) ...
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bioRxiv - Immunology 2022Quote: ... Real-time RT-PCR analyses for viral RNA copy number was carried out with One Step PrimeScript™ III RT-qPCR Mix (Takara, Cat# RR600B) and reactions were performed by using LightCycler® 96 System (Roche Diagnostics GmbH ...
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bioRxiv - Genetics 2020Quote: ... and reverse transcription (RT) was performed using PrimeScript RT reagent Kit with gDNA Eraser (TAKARA Bio, Kusatsu, Japan). Primers were designed to specifically amplify cDNA from transfected vectors and endogenous RPGR from 293T and ARPE-19 cells respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... These cell lines were tested negative for mycoplasma (TaKaRa PCR Mycoplasma Detection Set; 6601), maintained in RPMI1640 (Nacalai Tesque ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was carried out with the SYBR Premix Ex Taq II (TaKaRa) on Applied Biosystems Step One Real-Time PCR system ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative RT-PCR were carried out using TB Green Premix Ex Taq II (Takara). 1 μL of diluted cDNA was used for each reaction in a final volume of 15 μL ...
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bioRxiv - Plant Biology 2024Quote: ... The quantitative RT-PCR was performed on a Thermal Cycler Dice® TP800 (TaKaRa) with the SYBER® Premix Ex Taq TM II kit (TaKaRa ...
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bioRxiv - Immunology 2019Quote: ... Cell-death was assayed using LDH Cytotoxicity Detection Kit (Takara). To normalize for spontaneous lysis ...
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bioRxiv - Microbiology 2020Quote: ... and reverse transcribed using the PrimeScript RT reagent kit (TaKaRa). The GPC segment was amplified and sequenced as previously described (9) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated by PrimeScript RT Reagent Kit (Takara RR037A). Real-time PCR was performed on 15 ng of cDNA using SYBR Premix Ex Taq (Tli RNaseH Plus ...
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bioRxiv - Developmental Biology 2020Quote: ... and young adult) using the PrimeScript RT Reagent Kit (Takara). RT-qPCR was performed in a StepOnePlus(tm ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara). qPCR was conducted using SYBR Premix Ex Taq (Takara) ...
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bioRxiv - Genomics 2021Quote: ... and reverse transcribed using the PrimeScript RT Reagent Kit (Takara). qPCR was performed using primers listed in Table 1.
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bioRxiv - Cell Biology 2021Quote: ... The cDNA was synthesized using Primescript RT reagent kit (TakaRa), and qPCR analysis was carried out (14 ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated by the PrimeScript RT reagent Kit (TaKaRa), and quantitative qPCR was conducted using SYBR Green qPCR Master Mix (TaKaRa ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed using PrimeScriptTM RT reagent kit (Takara), qPCR was performed using the SYBR Green Premix Ex TaqTM (Takara ...
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bioRxiv - Immunology 2020Quote: ... and cDNA was obtained using the PrimeScript RT Kit (Takara). qPCR was performed using TaqMan primers and TaqMan Universal Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... we used PrimeScript RT reagent kit with gDNA Eraser (TAKARA) and superreal premix plus (SYBR Green ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated by RT Master reverse transcription kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... First strand cDNA was synthesized using PrimeScript RT kit (TakaRa). Optimized ddPCR was used to detect the presence of SARS-CoV-2 viruses following our previous study.10 Analysis of the ddPCR data was performed with QuantaSoft software (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized using the PrimeScript RT reagent kit (Takara). Real-time quantitative PCR was performed in triplicate using the SYBR Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using the PrimeScript RT Reagent Kit (Takara). Quantification of transcripts was performed on a Mx3005P QPCR system (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). Real-time qPCR experiments were performed as described above ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa). qPCR was performed using the SYBR Green Master Mix (High ROX Premixed ...
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bioRxiv - Genetics 2023Quote: ... RT was carried out with the PrimeScript Kit from TaKaRa. Quantitative RT-PCR reactions were carried out on a Real-time PCR machine (QuantStudio 5 by Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... cDNAs were synthesized using PrimeScript RT reagent Kit (Takara, Dalian) and then diluted and subjected to quantitative PCR using TransStart Green qPCR SuperMix (TransGen Biotch ...
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bioRxiv - Genetics 2023Quote: ... cDNA was synthesized using the PrimeScript RT reagent kit (Takara) and used as templates for real-time PCR analysis using SYBR PreMix ExTaqII (Takara) ...
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Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growthbioRxiv - Developmental Biology 2019Quote: ... Six DE miRNAs were reverse transcribed into cDNA using Mir-X™ miRNA First-Strand Synthesis Kits (Takara, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and a portion (1 ng) of the RNA were subjected to reverse transcription (RT) with a SMARTer™ PCR cDNA Synthesis Kit (Clontech, Mountain View, CA). Then ...
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bioRxiv - Neuroscience 2021Quote: Reverse transcription (RT) was performed using a PrimeScript® RT Reagent Kit with gDNA Eraser (RR047A, TaKaRa, Dalian, China) in a 20 μL reaction mixture containing 1 μg of total RNA from each individual sample ...
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bioRxiv - Plant Biology 2020Quote: Takara TB GreenTM Premix Ex TaqTM I and a Thermal Cycler Dice Real Time System TP800 (Takara) were used for both quantitative RT–PCR and quantitative PCR using the primers listed in Supplementary Table 1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and TATA-binding protein (TBP) (MA050367) were obtained from the Perfect Real-Time Supporting System (Takara Bio). The mRNA expression level was standardised to that of TBP ...