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Citations for Takara Bio :
51 - 100 of 3750 citations for bta mir 486 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... covering SIV env and nef were amplified from plasma viral RNA by nested RT-PCR using Prime-Script one-step RT-PCR kit v2 (TaKaRa) and KOD-Plus v2 (Toyobo) ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
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bioRxiv - Pathology 2023Quote: ... and reverse transcribed using PrimeScript RT Master Mix for RT-PCR (Takara Bio, Japan). Real-time PCR was performed using SYBR Select Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... One-Step PrimeScript RT-PCR Kit (Takara, RR064) was utilized for qRT-PCR (probe ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan)
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bioRxiv - Genetics 2023Quote: ... Then One-step PrimeScript RT-PCR kit (Takara) was used to generate cDNA ...
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bioRxiv - Genomics 2020Quote: ... 0.8μg of Total RNA was utilized as template for RT with random hexamer primers using PrimeScript RT reagent Kit (Takara). qRT-PCR was performed with respective gene-specific primers (PD GFD ...
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bioRxiv - Plant Biology 2020Quote: ... The remaining RT primers were digested on the beads with Exonuclease I (TAKARA) (37°C for 30 min ...
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bioRxiv - Genetics 2021Quote: Isolated RNA from OSF and control tissues was immediately subjected to realtime RT PCR (using Takara PrimescriptTM one step realtime RT PCR kit) (Takara, Japan) analysis for detection of changes in expression of NQO1 mRNA according to the manufacturers protocol ...
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bioRxiv - Microbiology 2020Quote: ... then subjected to quantitative real-time RT-PCR using a One-Step SYBR PrimeScript RT-PCR kit II (Takara Bio, Japan). The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38 ...
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bioRxiv - Microbiology 2022Quote: ... the viral RNA was isolated and amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio) and specific primers ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the OneStep TB Green PrimeScript PLUS RT-PCR kit (TAKARA, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... 90 μl of RNase free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara, Cat# RR096A) and primers for Nucleocapsid (N ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miR-122 or pre-miR-146a sequence cloned within pTRE-Tight-BI Vector (Clontech) in NheI and NotI sites or Bam HI and Hind III to make induced miR-122 (imiR-122 ...
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bioRxiv - Cell Biology 2021Quote: ... for the samples from the mouse heart or Human Mitochondrial DNA Monitoring Primer Set (TaKaRa Bio) for the cells following manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Pathology 2021Quote: ... Mycoplasma contamination was confirmed by PCR using the TaKaRa PCR Mycoplasma Detection Set (Takara, Shiga, Japan).
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Plastid double-strand RNA transgenes trigger small RNA-based gene silencing of nuclear-encoded genesbioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) was performed using Advantage RT-for-PCR kit (Takara, San Jose, CA) following the manufacturer’s instructions with 20 μM oligo (dT)18 ...
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bioRxiv - Biochemistry 2019Quote: ... RT was performed using Oligo(dT) random 6-mer primers from a Prime Script RT Master Mix kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA samples were reverse transcribed using an RT Primer Mix (oligo dT) and PrimeScript RT Enzyme Mix for qPCR (TaKaRa, Japan) following the manufacturer's protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 1× Universal Primer Mix (first PCR, Clontech Laboratories, Inc.) or 1× Nested Universal Primer (secondary PCR ...
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bioRxiv - Genomics 2019Quote: ... 0.48-μM IS PCR Primer (12 μM, Clontech, 639206), 2X Advantage 2 Polymerase Mix (50X ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was carried out using the One Step RNA PCR Kit (TaKaRa Biotechnology Dalian ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara; RR350A), while iTaq™ Universal SYBR® Green Supermix (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Real-time PCR was performed with SYBR RT-PCR kit (Takara, Dalian, China). The mRNA levels were normalized against β-Actin and presented as 2-ΔΔCt.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Real-time PCR was performed with SYBR RT-PCR kit (Takara, Dalian, China). The mRNA levels were normalized against β-Actin and presented as 2-ΔΔCt ...
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bioRxiv - Pathology 2020Quote: The viral loads of WHCV in BALF of patient 1 were determined by quantitative real-time RT-PCR with Takara One Step PrimeScript™ RT-PCR Kit (Takara RR064A) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The components of the master mix for the PCR and the PCR programs were set up according to the CloneAmp HIFI PCR protocol (Takara). All generated plasmids were sequenced before use (Eurofins Genomics).
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was performed using LA Taq (TAKARA Bio). The primer pairs employed for RT-PCR are listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... SYBR PrimeScript primary RT-PCR kit (Takara Bio, Japan) was used to evaluate the expression levels of METTL3 ...
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bioRxiv - Developmental Biology 2020Quote: ... retro-transcribed using the PrimeScript RT-PCR Kit (Takara) and analysed via qPCR using the SYBR qPCR Premix Ex Taq (Takara ...
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bioRxiv - Biochemistry 2020Quote: ... detected by the PCR Mycoplasma Detection Set (TAKARA BIO INC, Kusatsu, Japan).
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bioRxiv - Neuroscience 2024Quote: ... Cultures were tested regularly for mycoplasma (6601; TaKaRa PCR Mycoplasma Detection Set).
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bioRxiv - Microbiology 2023Quote: ... we used the following primer sets and In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) per the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of extracted RNA was subjected to one-step real-time RT-PCR using a One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (TaKaRa Bio Inc.) on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: The quantitative RT-PCR (qRT-PCR) was performed with SYBRGreen II (Takara, Dalian, China). The 7500 system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... The first PCR products were subsequently amplified by the nested Per2AS specific primers with Universal Primer (Clontech). The nested PCR products were cloned into pGEM-T vector (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... and complementary DNA of miR was synthesized using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa). RT-qPCR was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Neuroscience 2021Quote: ... or 1× Nested Universal Primer (secondary PCR, Clontech Laboratories, Inc.) and gene-specific primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The SARS-CoV-2 viral load was quantified in culture supernatant using RT-QPCR with primer probes targeting E gene of SARS-CoV-2 using PrimeDirect Probe RT-qPCR Mix (TaKaRa Bio USA, Inc) and Applied Biosystems QuantStudio3 real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse-transcribed using random primers (PrimeScript RT reagent kit with gDNA eraser, Takara). Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Plant Biology 2022Quote: ... (RT-qPCR) in the presence of SYBR Green (TaKaRa TB Green Primer Ex TaqII, #RR820Q) was carried out using a BioScript PCR kit (Bioline ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was amplified using the primer sets listed in Table S5 with SYBR Premix Ex Taq II (Takara) in accordance with the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed with PrimeSTAR GXL DNA polymerase (Takara) using gene-specific primers (Table S7) ...