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Citations for Takara Bio :
351 - 400 of 423 citations for Yeast Extracts since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech), followed by a selection of transformants on 2% SD (-Leu/-Trp ...
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bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
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bioRxiv - Plant Biology 2024Quote: ... two-hybrid assay was conducted according to the manufacturer’s instructions for the Match-maker Gold Yeast Two-Hybrid System (Clontech, USA). To generate the bait construct BD-GhCPK28 ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Cell Biology 2021Quote: Total genomic DNA of HeLa cells was extracted after irradiation using Total genomic DNA extract kit (TAKARA, China). Human nuclear GAPDH gene (forward ...
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bioRxiv - Immunology 2020Quote: ... The clarified extract was loaded onto a column containing TALON Metal Affinity Resin (BD Clontech, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and PCR inhibitors were removed from the DNA extracts using CHROMA SPIN+TE-400 Columns (Takara Bio, Inc.) and Amicon Ultra-0.5 (100K ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA was purified using the standard phenol-chloroform extract method and subjected to DNase I (Takara, 2270 B) treatment and reverse transcription for DRIPc-seq library construction ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Biochemistry 2020Quote: ... pOBD plasmids were then transformed into PJ69-4α yeast and were selected on tryptophan-dropout medium (Clontech, Mountain View CA, #630413); pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant constructs was transformed into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...
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bioRxiv - Physiology 2021Quote: ... the pSTT91 and pGAD424 plasmids cloned with potential interactors were introduced into the Saccharomyces cerevisiae strain L40 and grown for 2 days at 30°C on a leucine- and tryptophan- deficient synthetic defined premixed yeast growth medium (SD-Leu-Trp, TaKaRa Clontech)(Hollenberg et al. ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmid was used as bait to screen the cDNA library using the Matchmaker™ Gold Yeast Two-Hybrid Library Screening System kit (Cat. no. 630489, Clontech).
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bioRxiv - Plant Biology 2019Quote: Y1H assays were carried out using the Matchmaker Gold yeast one hybrid Library Screening System (Biosciences Clontech, Palo Alto, CA, USA). The PRH1 promoter was inserted into the pAbAi vector ...
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bioRxiv - Plant Biology 2019Quote: ... and the Gal4 AD fused with SV40 large T-antigen (pGADT7-T) from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as positive control ...
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bioRxiv - Plant Biology 2019Quote: ... The yeast containing Gal4 DNA-BD fused with lamin (pGBKT7-Lam) and the pGADT7-T from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as a negative control.
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bioRxiv - Plant Biology 2019Quote: ... HYL1 and DDL1 were prepared in pGADT7 and pGBKT7 and the experiment was performed following the protocol of Matchmaker® Gold Yeast Two-Hybrid system (Clontech). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Mated yeast clones were selected using minimal synthetic defined (SD) medium with double dropout (Leu− and Trp−) supplement (Clontech Takara Bio) containing Aureobasidin A and X-α-Gal as the blue-colored colonies ...
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bioRxiv - Neuroscience 2020Quote: ... Mated yeast clones were selected using minimal synthetic defined (SD) medium with double dropout (Leu− and Trp−) supplement (Clontech Takara Bio) containing Aureobasidin A and X-α-Gal as the blue-colored colonies ...
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bioRxiv - Plant Biology 2021Quote: ... The Y2H assay was performed following the manual provided in the kit “Matchmaker Gold Yeast Two-Hybrid” (Clontech, Mountain View, USA). The list of VirE2 interacting clones has been given in Fig.S1.
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bioRxiv - Biophysics 2020Quote: ... Transformed yeast cells were cultured at 30°C in uracil dropout synthetic defined medium (-Ura dropout supplement was purchased from Takara Bio). To induce expression of genes under the control of the GAL1 promoter ...
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bioRxiv - Microbiology 2021Quote: ... The amplified PCR product was verified by sequencing and then cloned into modified XW55 yeast dual expression vector65 in the highlighted SpeI and PmlI sites downstream of the ADH2 promoter by yeast recombinase technology [protocolYeastmaker™ YeastTransformation System 2 (Clontech)] and according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... StNPR1 or StNPR3/4 cds) construct combinations were transformed into the Y2H Gold strain using the Matchmaker Gold Yeast Two-Hybrid System (Clontech, USA) and the transformants selected on control SD media without Leu and Trp (-L-W) ...
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bioRxiv - Plant Biology 2022Quote: ... in pGADT7 were combined with each other and with empty control plasmids using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The vectors were co-transformed into the Y2Hgold MATa Yeast strain ...
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bioRxiv - Plant Biology 2022Quote: ... The yeast strain Y2H AH109 was cotransformed with the prey and corresponding bait vectors following the protocol provided by the Yeastmaker™ yeast transformation system (Clontech). The yeast transformants were grown on SD/-Trp/-Leu medium ...
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bioRxiv - Plant Biology 2023Quote: OsbZIP1.1 and OsbZIP1.2 CDS were cloned in pDEST-GBKTT7 (CD3-764) obtained from TAIR (https://www.arabidopsis. org) and transformed in the AH109 yeast strain (Clontech, TaKaRa Bio, USA). Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant constructs were introduced into Y1H gold strain and Y1H assay was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: The recombinant pGBKT7-PGRP3 bait construct was transformed into yeast strain Y2HGold via the lithium acetate method per manufacturer protocol (Takara Bio). Transformants were cultured on SD/-Trp (SDO ...
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bioRxiv - Cell Biology 2023Quote: ... and of human FAM104B isoform 3 (NM_001166700.2) were PCR- amplified from a yeast two-hybrid human testis cDNA library (Clontech, cat. no. 638810) and cloned via appropriate restriction sites into pGAD-C1 (James et al ...
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bioRxiv - Plant Biology 2023Quote: ... were used to co-transform yeast strain AH109 and colonies carrying both vectors were selected on SD medium without tryptophan (-W) and leucine (-L) (TaKaRa 630317) at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... lysed by sonication and the recombinant His-tagged protein purified from cell-free extracts using IMAC (Talon resin, Clontech) as previously described19.
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bioRxiv - Neuroscience 2022Quote: ... coli cells induced with 100 mM IPTG and purified from bacterial extracts using TALON beads (Takara Bio, Catalog # 635502) according to the vendor’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Verification of positive colonies was achieved by co-transforming wild-type or YR-mutant TMEM39A loop domain (in pGBKT7 Vector) with genes of interest (in pGADT7 Vector) following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) as well as plasmids from re-cloned cDNA.
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bioRxiv - Cell Biology 2020Quote: The yeast strain AH109 was co-transfected with bait vector full-length human SNX4 or Lamin cloned into pGBKT7 (Clontech, Oxford, UK) against a pray library of FL SNX-BARs ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... NRC3 and NRC4 CC and NB-ARC domain truncations and ran a yeast two-hybrid experiment using the Matchmaker Gold system (Takara Bio USA). Plasmid DNA encoding AVRcap1b (negative control ...
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bioRxiv - Plant Biology 2021Quote: ... For expressing the GAL4BD-SMXL5 fusion protein in yeast the open reading frame of the SMXL5 gene was cloned into XmaI/BamHI sites of the pGBKT7 plasmid (Clontech, Palo Alto) resulting in pEW6 ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid techniques were performed according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany) using the yeast reporter strains AH109 and Y187 ...
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bioRxiv - Plant Biology 2021Quote: ... Y2H screen was performed to screen the apple library according to the user manual of Matchmaker™ Gold Yeast Two Hybrid System (Clontech, Japan) by using Saccharomyces cerevisiae strain Y2H Gold.
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bioRxiv - Genetics 2020Quote: ... Verification of positive colonies was achieved by co-transformation of extracted bait and prey plasmids following the instruction of YeastMaker™ Yeast Transformation System 2 (Clontech, 630439) and by bait/prey plasmids with re-cloned cDNA.
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bioRxiv - Plant Biology 2020Quote: ... and LacZ reporter genes was examined with yeast AH109 transformants on SD/−Trp/−His and SD/−Trp/−Ade media (Clontech Inc., USA). The fix composition of SD plates contained 0.17 g yeast nitrogen base (YNB) ...
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bioRxiv - Cell Biology 2022Quote: ... Matings between the Y2HGold and Y187 strains carrying the appropriate constructs were performed in 0.5 mL of 2X YPDA in the presence of the corresponding antibiotics following manufacturer’s instructions (Clontech’s Matchmaker Gold Yeast Two-Hybrid System, Clontech by Takara Bio, Mountain View, USA). Yeast cells were cultured at 30 ºC for 24 h and plated in SD-Trp/-Leu/X-alpha-Gal agar plates ...
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bioRxiv - Cell Biology 2022Quote: ... positive control (pGBKT7-53) and negative control bait plasmids (pGBKT7-Lam) were transformed into yeast strain Y2HGold (Takara Bio, Mountain View, USA) while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: Direct protein-protein interaction was tested by Y2H technique according to the yeast protocols handbook and the Matchmaker GAL4 Two-hybrid System 3 manual (both Clontech, Heidelberg, Germany). Yeast strain Y190 was co-transformed with respective plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD) of pGADT7-Rec2 (Clontech, CA, USA) prey vector ...
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bioRxiv - Plant Biology 2023Quote: A Y2H library screen was performed as outlined in the Matchmaker Gold “Mate and Plate” Yeast Two-Hybrid System (Takara Bio Inc.) using pGBKT7-CML13 as the bait in yeast strain AH109 ...
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bioRxiv - Plant Biology 2023Quote: OsbZIP1.1 and OsbZIP1.2 CDS were cloned in pDEST-GBKTT7 (CD3-764) obtained from TAIR (https://www.arabidopsis. org) and transformed in the AH109 yeast strain (Clontech, TaKaRa Bio, USA). Yeast transformation was performed as described in the Yeastmaker Yeast Transformation System 2 User Manual (Clontech ...
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bioRxiv - Plant Biology 2023Quote: For Yeast-two-Hybrid (Y2H) experiments coding DNA sequences were cloned into pDONR207 and recombined into pGDAT7® or pGBKT7® vectors (Clontech). Plasmids were transformed into the AH109 yeast strain by the lithium acetate (LiAc ...