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Citations for Takara Bio :
101 - 150 of 177 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and DNA templates were digested by RNase-free DNase I (Takara Bio, Shiga, Japan).
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bioRxiv - Immunology 2022Quote: ... Lenti-X 293T packaging cells were obtained from Takara-Clontech and were cultured in DMEM supplemented with 10% tetracycline-free FBS (Takara-Clontech), 200 mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... Lenti-X 293T packaging cells were obtained from Takara-Clontech and were cultured in DMEM supplemented with 10% tetracycline-free FBS (Takara-Clontech), 200 mM L-glutamine ...
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bioRxiv - Neuroscience 2022Quote: AAV vectors (serotype DJ) were generated using the pHelper (AAVpro Helper-free system, Takara), pAAV-DJ (Cell Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa-KRAS(G12V) cells were maintained in 10% tetracycline-free FBS (Takara Bio, 631101) and KRAS(G12V ...
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bioRxiv - Cell Biology 2022Quote: ... and the samples were homogenized using an RNase-free tube and plunger (Takara, 9791A) and then vortexed for 5 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... Greenberg) were cultured in DMEM supplemented with 10% tetracycline-free fetal bovine serum (Takara) and antibiotics ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells were grown in HEK293T medium except 10% tetracycline-free FBS (Takara Bio) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... all supplemented with 10% tetracycline-free Fetal Bovine Serum (FBS) (Clontech and DSS Takara). Cells were trypsinized and sub-cultured in desired culture vessels when at 90% confluency ...
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bioRxiv - Cancer Biology 2023Quote: ... all supplemented with 10% tetracycline-free Fetal Bovine Serum (FBS) (Clontech and DSS Takara). Cells were trypsinized and sub-cultured in desired culture vessels when at 90% confluency ...
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bioRxiv - Immunology 2024Quote: ... and purified using the NucleoBond Xtra Midi endotoxin-free midi-prep kit (Takara Bio). For immunocytokines ...
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Exometabolite dynamics over stationary phase reveal strain-specific responses to nutrient limitationbioRxiv - Microbiology 2020Quote: ... filter plates were washed three times with sterile water using a vacuum apparatus (NucleoVac 96 vacuum manifold; Clontech Laboratories). The filter of well H12 was removed with a sterile pipette tip and forceps ...
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bioRxiv - Microbiology 2023Quote: ... filter plates were washed three times with sterile water using a vacuum apparatus (NucleoVac 96 vacuum manifold; Clontech Laboratories). The filter of well H12 was removed with a sterile pipette tip and tweezer ...
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bioRxiv - Genomics 2021Quote: ... U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech), 100U/ml penicillin and 100µg/ml streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... hygromycin B (300 µg/ml) and 10% tetracycline free fetal bovine serum (Takara Bio, USA) in a humidified incubator at 37°C in 5% CO2 in 6-well plates ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Immunology 2024Quote: ... the isolated RNAs underwent treatment with RNase-free DNase I (Takara Bio, Otsu, Shiga, Japan) to remove any contaminating DNA ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 200 uL Template Switch mix (88 uL water, 44 uL 5x Maxima buffer, 44 uL 20% Ficoll PM-400, 22 uL 10 mM Takara dNTPs ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated RNA was dissolved in 10 μl of DEPC-treated water and reverse-transcribed using a reverse transcription reagent kit (PrimeScript RT Reagent Kit with gDNA Eraser, Takara) and a thermal cycler (Mastercycler ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA contamination was removed by treating it with RNase-free DNase I (Takara Bio, China). Approximately 1 μg of total RNA was used for the first-strand cDNA synthesis using RevertAid cDNA synthesis kit (Thermo Fischer Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The trace amount of DNA was removed through incubation with RNase-free DNase I (TaKaRa, Japan) at 28 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from 500 ng of DNA-free RNA using PrimeScript RT reagent Kit (Takara), supplemented with RNase out (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... prepared freshly with 940 nL of Lysis Buffer 1 (1 μL of 10X reaction buffer is diluted in 2.75 μL of water) (SMART-Seq v4 Ultra Low input RNA kit; Takara Bio Inc.) per well ...
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A New Gene Set Identifies Senescent Cells and Predicts Senescence-Associated Pathways Across TissuesbioRxiv - Cell Biology 2021Quote: ... 86% of 2% Tween 20 in deionized Water) or AP20187 (B/B homodimerizer, Clontech; 10 mg of AP20187 per kg body mass) twice weekly at the age of 20 months for a total of 4 months (old mice were sacrificed at 24 months of age) ...
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bioRxiv - Microbiology 2021Quote: ... RNA-free cDNA (5μL) was used as a template for PCR with PrimeStar GXL Polymerase (Takara, Japan) and back-to-back PCR primers (LigSeq-F and LigSeq-R ...
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bioRxiv - Bioengineering 2021Quote: ... an endotoxin-free plasmid midiprep DNA purification took place using NucleoBond Xtra Midi EF kit (Takara Bio) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HuEpi library was sequenced and contains all 5,309 sgRNAs included in the synthesis (GSE215430).
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Biochemistry 2022Quote: ... were purchased from Biomatik and cloned into BstBI-digested PBRM1-TetO-FUW using the ligation-free In-Fusion HD cloning kit (Takara). The construct was packaged into lentivirus using HEK293T cells and delivered into target cells together with pLenti CMV rtTA3 Hygro (w785-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA to generate viral vectors was prepared using the NucleoBond Xtra Maxi endotoxin free kit (Takara Bio) prior to production ...
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bioRxiv - Microbiology 2022Quote: ... 2X ProteoGuard EDTA-free protease inhibitor cocktail (catalog no. 635673, Takara Bio USA, Inc., San Jose, CA, USA), and DNase and RNase-free RPI molecular grade water (catalog no ...
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bioRxiv - Neuroscience 2021Quote: ... All the surfaces of the environment were kept clean and RNase-free with DNA-OFF (Takara Cat. #9036) and RNase Zap (Life Technologies Cat ...
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bioRxiv - Cell Biology 2022Quote: ... was added per 50 mg feces and then homogenized using an RNase-free tube and plunger (Takara, 9791A). 100 µL chloroform (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: All cells were cultured in high glucose DMEM with 10% Fetal Bovine Serum (tetracycline free, Clontech or Gibco) and penicillin/streptomycin 50units/ml-50 mg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... KhES-1 was cultured at feeder-free conditions using StemFit® AK02N (Catalog #RCAK02N, TAKARA BIO, Kusatsu, Japan) with daily medium change ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 g RNA was used to synthesize the cDNA using reverse transcription M-MLV (RNase-free) kits (TaKaRa, Japan). qRT-PCR was performed with SYBR green PCR master mix (ToYoBo ...
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bioRxiv - Microbiology 2020Quote: ... lysed by sonication and the recombinant His-tagged protein purified from cell-free extracts using IMAC (Talon resin, Clontech) as previously described19.
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bioRxiv - Molecular Biology 2022Quote: ... the cell pellets were resuspended in the prepared cell lysis buffer (0.1% Triton-X, 20 mM Tris, 100 mM NaCl) and ProteoGuard EDTA-Free protease inhibitor cocktail (Takara) for 30 minutes followed by sonication in ice cold water for 40 minutes (75 amplitude ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were maintained at 37 °C in a humidified atmosphere at 5% CO2 in DMEM 4.5g/L Glucose with UltraGlutamine media supplemented with 10% of Tet-free FBS (Clontech) and 1% penicillin/streptomycin.
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bioRxiv - Neuroscience 2023Quote: ... GP2-293 cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% tetracycline-free FBS (Takara Bio).
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bioRxiv - Systems Biology 2020Quote: ... RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio USA, Mountain View, CA). 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases, Takara Bio DO Supplement -His/-Leu). Cultures were grown to saturation ...
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bioRxiv - Microbiology 2021Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Cells were seeded at 25-30% confluency onto the prepared coverslips placed in 6-well plates in cell imaging media comprising phenol-red free DMEM (HyClone) supplemented with 10% (v/v) FBS (ClonTech) and 1 mM sodium pyruvate and allowed to settle for at least 4h ...