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Citations for Takara Bio :
201 - 237 of 237 citations for Val5 Angiotensin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... with 5’-cgGAATTCaccATGgagcagaagctc atctcagaagaagacctcggtAAGGATAACACCGTGCC-3’ and 5’-ataagaatGCGGCCGCtaaact ATTATTTTTCTGCACTACGCAGG-3’ followed by cloning into the EcoRI and Not I sites of pIRESpuro3 (Clontech) creating pIRESpuro3-myc-BirA ...
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bioRxiv - Microbiology 2020Quote: ... The restriction enzyme digested DNA fragments were purified and then cloned to the corresponding sites in pCold-I vector (TAKARA) followed by transformation to E ...
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bioRxiv - Genetics 2020Quote: ... the pooled cDNA samples of human foetal tissues (Fetal MTC Panel, 636747, Lot#1308272A) and adult tissues (MTC panel I, 636742, Lot#1303120A) were sourced from Clontech. The cDNA samples from whole human embryonic and foetal hearts of different developing stages were obtained from the MRC-Wellcome Human Developmental Biology Resource (HDBR) ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR purified integrin β5 with engineered flanking restriction sites (Xho-I/BamHI) was subcloned into the multi-cloning sites of pEGFP-N1 (Clontech) to encode an in-frame fusion protein with the carboxy-terminal EGFP-tag (pEGFP-N1-Integrin β5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA of the RepNDK-RNA clone and the plasmids for all other RNA clones were subjected to digestion with Sma I (Takara) and in vitro transcription with T7 RNA polymerase (Takara) ...
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bioRxiv - Genetics 2023Quote: ... The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara). PCR conditions were optimized for long fragments as follows ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA was isolated from cells using TRIzol Reagent following the instructions and genomic DNA was removed using DNase I (TaKara). Subsequently ...
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bioRxiv - Plant Biology 2023Quote: ... diluted to 1 µg/mL with Milli-Q ultra-pure water for 5 min at room temperature or with a pollen germination medium containing 2000-fold SYBR Green I (Cat#: 5760A, Takara) and 5 µg/mL DAPI (Cat# 10236276001 ...
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bioRxiv - Microbiology 2023Quote: ... These three amplified fragments were then seamlessly integrated into the Sac I site of YEP352-GAPII (Oka et al. 2007) using the In-Fusion HD Cloning Kit (Takara). Similarly ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using RNApure FFPE Kit (CWBIO, Jiangsu, China) and then treated with DNase I (TaKaRa, Kyoto, Japan) to remove DNA contaminants ...
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bioRxiv - Plant Biology 2023Quote: ... between the pU6 promoter and sgRNA scaffold via Bsa I digestion and T4 DNA ligase-mediated ligation (Takara, cat. 6023). For oligonucleotide-dependent mutation knock-in ...
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bioRxiv - Biochemistry 2024Quote: ... The codons S85 and N86 were designed as the recognition sequence (TTCGAA) of BspT104 I (Takara Bio Inc., Shiga, Japan) for further modification of the linker insertion ...
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bioRxiv - Biophysics 2019Quote: ... NG-Scarlet and NG-Cherry were created by deleting mRuby3 from NG-Ruby3 by inverse PCR and insertion of either mScarlet-I or mCherry using In-Fusion cloning (Takara Bio). NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... and inserted into Bgl II/Sal I-linearized pL7 mGFP or pL7 mCherry (Wagner, McCroskery and Hammer, 2011) using an In-Fusion cloning kit (Takara, 638920). The specific fragments generated using the appropriate template and indicated primers were ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were treated for 1 h by DNase I at 37°C and reverse-transcribed using a Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed as mentioned above ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was then performed using a Bio-Rad CFX-96 PCR detection system (USA) and a SYBR Green I mixture (Takara, Beijing). The MRSA housekeeping gene gyrB was used as an internal reference for normalization of the data.
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bioRxiv - Microbiology 2021Quote: ... The amplified fragments were cloned into the SmaI site of pPTR I using an In-Fusion HD cloning kit (Takara Bio). Transformants were selected on minimal agar medium with pyrithiamine.
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bioRxiv - Microbiology 2021Quote: ... The amplified fragment was cloned into the SmaI site of pPTR I using an In-Fusion HD cloning kit (Takara Bio). The pPTR I-GFP-nrdA plasmid was used to transform the A ...
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bioRxiv - Neuroscience 2019Quote: ... after which the pellet was re-suspended in 500 μL DMEM and added with 5 μL DNase I solution (Takara, M0303L) for a 30 min incubation at room temperature to avoid the viral particles aggregation ...
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bioRxiv - Biochemistry 2022Quote: The expression plasmid for N-terminal 6×His-tagged mCherry-Nter was constructed by inserting the sequence encoding DmAgo2 Nter (residues 1–398) amplified from the native DmAgo2 sequence into pCold I (TAKARA Bio) using the InFusion HD cloning kit (Clontech) ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was extracted using the TRIzol® reagent (R0016, Beyotime, Shanghai) followed by the removal of genomic DNA using DNase I (TaKara). The 16S and 23S ribosomal RNA (rRNA ...
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bioRxiv - Microbiology 2023Quote: ... Total RNAS were extracted from these sediments using TRIzol® Reagent according the manufacturer’s instructions and genomic DNA was removed using DNase I (TaKara, Japan). Then RNA quality was determined using 2100 Bioanalyser (Agilent ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA used for qRT-PCR were synthesized using PrimeScript reverse transcriptase with oligo dT primer and Prime Script RT Enzyme MIX I (Takara, Osaka, Japan). qRT-PCRs was performed using the ChamQ SYBR Color qPCR Master Mix (Q411 ...
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bioRxiv - Plant Biology 2021Quote: ... with each primer set and cloned into the BamHI and Hind III sites of the pCold I vector by In-Fusion Cloning (Takara Bio, Japan). The OsGH3-8 ORF was synthesized according to the protein sequence of OsGH3.8 in database [XP_015647797] and cloned into pCold I vector ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total RNA was extracted using Sepasol-RNA I Super G (Nacalai Tesque) according to the manufacturer’s instructions and reverse-transcribed using PrimeScript Reverse Transcriptase (Takara Bio, Shiga, Japan). Expression of cytokines was quantified using a Thermal Cycler Dice Real Time System (Takara Bio ...
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bioRxiv - Plant Biology 2019Quote: The isolated RNA by using the modified phenol chloroform method as previously described [33] was treated with RNase-free DNase I (TaKaRa, Dalian, China) at 37°C for 1 h to eliminate DNA contamination in real-time quantitative RT-PCR analysis ...
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bioRxiv - Plant Biology 2022Quote: ... Gaillardia × grandiflora ‘Arizona Apricot’ and Antirrhinum majus using Sepasol RNA I Super G (Nacalai Tesque) and purified with a high-salt solution for precipitation (Takara Bio Inc.). A five-micro gram of total RNA was used for the gel blot analysis ...
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bioRxiv - Plant Biology 2023Quote: Total RNAs from inflorescences or seedlings were treated with DNase I followed by reverse transcription using PrimeScript™ II 1st Strand cDNA Synthesis Kit (TAKARA, 6210A) with oligo-d(T ...
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bioRxiv - Genomics 2024Quote: We applied three distinct cDNA library enrichment protocols for the RNA sample extracted from the same mice individual: i) standard PacBio Clontech SMARTer PCR cDNA Synthesis kit (Clontech Laboratories, Inc.); ii ...
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bioRxiv - Biochemistry 2021Quote: The amplified DNA products of the expected size were digested with the appropriate restriction enzymes and cloned into the pCOLD I expression vector (Takara Bio, Kusatsu, Japan), which allows for the production of proteins with an N-terminal His6 tag ...
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bioRxiv - Microbiology 2020Quote: pEX18Tc-hpdA was constructed for gene knockout by fusing the erythromycin resistance gene and two upstream and downstream fragments of the target gene amplified with the primers shown in Table 3 to Sac I/Hind III-digested pEX18Tc with the In-Fusion® HD Cloning Kit (TaKaRa, Dalian, China). The resulting plasmid pEX18Tc-hpdA was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was subjected to three freeze-thaw cycles at -80 °C and 37 °C and treated with 5 units of DNase I (TaKaRa Bio, Otsu, Japan) and 20 ng RNase (Nippon Gene ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extraction and genomic DNA removal were performed using a Total RNA Extraction Kit (Plant) (RBC Bioscience, New Taipei, Taiwan) and DNase I (Takara Bio, Shiga, Japan) following the manufacturers’ protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories, Inc., Kusatsu, Japan) to generate the bait vectors ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted by using Sepasol®-RNA I Super G (nacalai, Tokyo, Japan) according to the manufacturer’s instructions and DNase-treated (Takara Bio Inc., Tokyo, Japan). cDNA was synthesized using SuperScript VILO (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The EcoRI sites of the ChR2(T159C)-EYFP fragment was blunt ended with the Klenow fragment of Escherichia coli DNA polymerase I (Takara Bio Inc., Shiga, Japan, 2140A). The resulting ChR2(T159C)-EYFP fragment was subcloned into pLenti-TRE3G::IRES at the BglII-EcoRV restriction sites ...