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Citations for Takara Bio :
51 - 62 of 62 citations for Val4 Angiotensin III since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were PCR-re-amplified and finally subcloned into Hind III site of the pEYFP-C1 or pECFP-C1 vectors (Clontech).
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bioRxiv - Genomics 2020Quote: ... pseudoviridinutans was extracted from a 2-d-old culture using phenol-chloroform and NucleoBond buffer set III (TaKaRa, Shiga, Japan). The DNA was fragmented in an S2 sonicator (Covaris ...
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bioRxiv - Microbiology 2023Quote: ... We previously described the generation of psiRNA-U6GFP::Zeo and the nonsense negative control psiRNA-U6GFP::Zeo-shNS from the U6 RNA Polymerase III promoter in pSIREN-shuttle (Clontech) and psiRNA- 7SKGFP::Zeo (InvivoGen ...
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bioRxiv - Microbiology 2023Quote: ... and quantified by RT‒qPCR using Primer/Probe N2 (NIHON GENE RESEARCH LABORATORIES, INC. Miyagi, Japan) and One Step PrimeScript™ III RT‒qPCR Mix (TaKaRa) with CFX96 (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... was done using a SYBR Premix Ex TaqII Dimer Eraser and a Thermal Cycler Dice Real Time System III (Takara Bio). The gene encoding elongation factor 1α (EF1α ...
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bioRxiv - Microbiology 2024Quote: The WOo DNA methyltransferase was artificially synthesised with codon usage adaptation and cloned into the XhoI and SalI sites of the pCOLD III expression vector (Takara Bio). The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm– ...
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bioRxiv - Plant Biology 2021Quote: ... with each primer set and cloned into the BamHI and Hind III sites of the pCold I vector by In-Fusion Cloning (Takara Bio, Japan). The OsGH3-8 ORF was synthesized according to the protein sequence of OsGH3.8 in database [XP_015647797] and cloned into pCold I vector ...
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bioRxiv - Microbiology 2020Quote: pEX18Tc-hpdA was constructed for gene knockout by fusing the erythromycin resistance gene and two upstream and downstream fragments of the target gene amplified with the primers shown in Table 3 to Sac I/Hind III-digested pEX18Tc with the In-Fusion® HD Cloning Kit (TaKaRa, Dalian, China). The resulting plasmid pEX18Tc-hpdA was transformed into E ...
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bioRxiv - Immunology 2022Quote: ... Real-time RT-PCR analyses for viral RNA copy number was carried out with One Step PrimeScript™ III RT-qPCR Mix (Takara, Cat# RR600B) and reactions were performed by using LightCycler® 96 System (Roche Diagnostics GmbH ...
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bioRxiv - Microbiology 2023Quote: ... and real-time RT-PCR was performed by targeting the SARS-CoV-2 RdRp gene as follows: each 20 µl sample consisted of 12.5 µl One Step PrimeScript™ III RT-PCR Kit (Takara Bio, Shiga, Japan), 0.5 µM Sars-CoV-2 CRV forward primer (5’-TCACCTAATTTAGCATGGCCTCT-3’) ...
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bioRxiv - Physiology 2023Quote: ... The cDNA of each mouse gene was amplified and detected using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) and Thermal Cycler Dice Real Time System III (TAKARA BIO, Shiga, Japan). 18S ribosomal RNA was used for the reference gene ...
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bioRxiv - Microbiology 2022Quote: RT-PCR was performed in a single closed tube using a one-step RT-PCR kit (One Step PrimeScript III RT-qPCR Mix, with UNG; Takara Bio Inc., Kusatsu, Japan) in accordance with the manufacturer’s instructions ...