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Citations for Takara Bio :
401 - 450 of 557 citations for Urotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Real-time quantification PCR was performed using the SYBR Premix Ex Taq II (Tli RNase H Plus) (Takara, Japan) with the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was reverse transcribed with a PrimeScript II 1st strand cDNA Synthesis Kit (no. 6210A; Takara Bio, Kyoto, Japan). Based on the information at RefSeq (http://www.ncbi.nlm.nih.gov/RefSeq) ...
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bioRxiv - Plant Biology 2022Quote: ... PCR reaction mixture was prepared at a final volume of 20µl which contains 10 µl of TB Green Premix Ex Taq II (Takara), 10pM of forward and reverse primers and 2µl of diluted cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Viral load was measured by RT-qPCR using One-Step SYBR® Primescript(tm) RT-PCR kit II (Takara). CT values from serum samples were used to calculate serum viral load according to regression equation built by a set of standard viral RNA extracted from dilutions of known titre virus preparation ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR analysis was performed with Light Cycler-480®II (Loche) using TB-Green (Takara, RR420) following the supplier’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... The reactions were carried out using TB Green® Premix Ex Taq™ II (TAKARA-Bio, Cat no. RR82WR) and incubated at 95 °C for 2 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using a Thermal Cycler Dice Real Time System II (Takara Bio Inc., Otsu, Japan) and SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Microbiology 2024Quote: ... was amplified with primers Fe-227S and Fe-204R using SYBR Premix Ex Taq II (Tli RNaseH Plus; Takara) (Table 2) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then cDNA was synthesized according to the PrimeScript TM II 1st Strand cDNA Synthesis Kit (Takara Bio, Dalian, China). Real-time PCR analysis was performed with the LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed using a Thermal Cycler Dice Real-Time System II TP900 (Takara Bio, Shiga, Japan), and the relative copy number of various gene transcripts per beta-actin transcript was determined using calibration standards for each tested molecule ...
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bioRxiv - Cancer Biology 2024Quote: ... and the remaining RNA isolation was done using the NucleoSpin RNA II Kit (Clontech Laboratories, Palo Alto, CA; 740955.50). 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... PrimeScript® RT Master Mix Perfect Real-Time and DRR820ASYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... at the C-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) for a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Molecular Biology 2020Quote: ... a fluorescent quantitative PCR was carried out in accordance with the manufacturer’s instructions of a SYBR® Premix Ex TaqTM II (Tli RNaseH Plus) kit (Takara). The primers were synthesized by RiboBio Company and were shown in Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of these mRNAs was assayed using TB Green™ Premix Ex Taq™ II (Tli RNase H Plus; Takara) according to manufacturer’s instructions with QuantiTect primer assays (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: Total RNA was collected from iPSC-derived day 12 cExN and cIN NPCs using the NucleoSpin RNA II kit (Takara) per the manufacturer’s instructions ...
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A Plant-Specific Polarity Module Establishes Cell Fate Asymmetry in the Arabidopsis Stomatal LineagebioRxiv - Plant Biology 2019Quote: ... Directed yeast two-hybrid constructs were generated by ligation of cDNAs into vector components of the Matchmaker II system (Clontech). Bait and prey clones were cotransformed into yeast strain AH109 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resultant cDNA was subjected to real-time PCR by using SYBR® Premix Ex Taq™ II (Takara Bio) and specific primer sets (Takara Bio) ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR analysis was performed with 5ng of template gDNA in a 25µl reaction using the TB Green Premix Ex Taq II (Takara, #RR820L) and the CFX Connect (Biorad CFX Connect ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCR was performed as previously described using TB Green Premix Ex Taq II (Takara Bio Inc, Japan) and a QuantStudio 7 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with T7-oligo(dT)24 as a primer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Then the poly(A ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA and miRNA from three independent biological samples were reverse transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) and the miRcute Plus miRNA First-Stand cDNA Synthesis Kit (Tiangen) ...
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bioRxiv - Microbiology 2020Quote: ... The viral load was measured by RT-qPCR using One-Step SYBR® Primescript™ RT-PCR kit II (Takara). CT values of serum samples were used to calculate serum viral titre according to regression equation built by RNA extracted from 10 µL of 102-106 pfu/mL of ZIKV (PRVABC59 or MP1751) ...
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bioRxiv - Cell Biology 2020Quote: ... 3C protease-cleavage site and a His12-tag at the N-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time reverse transcription-PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (TaKaRa, Japan) with the LightCycler® 96 system (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using 1/60 of the immunoprecipitated DNA and the TB Green Premix Ex Taq II (Takara). Relative quantification using standard curve method was performed for each primer pair and 96-well Optical Reaction plates were read in a StepOnePlus PCR instrument (Applied Biosystem) ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
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bioRxiv - Plant Biology 2021Quote: ... which was used to synthesize first-strand cDNA using the SuperScript II first-strand cDNA synthesis Kit™ (Takara, Japan). The Arabidopsis Actin2 gene was used as the internal reference gene.
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bioRxiv - Evolutionary Biology 2022Quote: ... 100 ng RNA extracted from 12 male or female pharate larvae was reverse-transcribed using PrimeScript II reverse transcriptase (TaKaRa) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: The Yeast-Two-Hybrid screening for Jacob interaction partners was performed using a pretransformed human brain cDNA Library in pACT2 (Matchmaker-GAL4 Two-Hybrid II; Clontech) as described previously (Helmuth et al. ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 ng RNA was reverse transcribed into complementary DNA (cDNA) using a PrimeScript II 1st Strand cDNA Synthesis kit (TaKaRa) or a QuantiNova Reverse Transcript Kit (Qiagen ...
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bioRxiv - Cell Biology 2019Quote: ... The coding sequences of these small GTPase proteins were amplified with polymerase chain reaction (PCR) using KOD-Plus-Neo DNA polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as template cDNA ...
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bioRxiv - Cell Biology 2020Quote: Four hundred ng total RNA was employed for cDNA synthesis using ProtoScript II First Strand cDNA Synthesis kit(TaKaRa,#RR036A) according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA was used to synthesize cDNA with a PrimeScript II 1st Strand cDNA Synthesis Kit (D6210A; TaKaRa, Dalian, China) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... 5 µL of 1:10 diluted cDNA samples was used as the qRT-PCR template with 0.5 µM gene-specific primers and 10 µL SYBR Premix Ex Taq II (Takara, China) in a total volume of 20 µL ...
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bioRxiv - Neuroscience 2021Quote: Total RNAs from the mouse forebrain were reverse transcribed using the PrimeScript II first strand cDNA Synthesis Kit (Takara Bio) with the random hexamer primer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fluorescence intensities of bands corresponding to parasitic RNAs were quantified using ImageJ (NIH) after staining with SYBR Green II (Takara). The concentrations of parasitic RNAs were determined from the intensities based on a dilution series of a standard parasitic RNA (s222 [35]).
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bioRxiv - Synthetic Biology 2022Quote: ... qPCR was conducted with 10ng genomic DNA in duplicate in a 20 μl reaction using the TB Green Premix Ex Taq II (Tli RNase H Plus, Takara) and the StepOnePlus™ Real-Time PCR System (ABI) ...
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bioRxiv - Microbiology 2023Quote: ... and were quantified by real-time RT-PCR using One Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa) and QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNAs (1-5 ng RNA equivalents) were used for real-time PCR amplification using SYBR Premix Ex Taq II (Takara/Clontech) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2022Quote: ... was inserted upstream of a thrombin protease cleavable linker (LVPRGS) and Strep II epitope (WSHPQFEK) in a modified pFastBac vector by In-Fusion cloning (Clontech). Mutagenesis using the QuikChange Site-Directed Mutagenesis kit (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... and those targeting the Alu repeat sequence in the nuclear genome (5′-CTTGCAGTGAGCCGAGATT-3′ and 5′- GAGACGGAGTCTCGCTCTGTC-3′) (75) with TB Green Premix Ex Taq II (TaKaRa) on Thermal Cycler Dice Real Time System II (TaKaRa) ...
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bioRxiv - Microbiology 2023Quote: ... The resultant reaction mixture was then 10-fold diluted and subjected to quantitative PCR using TB Green Premix Ex Taq II kit (TaKaRa) in combination with a tag primer (no ...
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bioRxiv - Microbiology 2023Quote: ... cDNA copies of IBVs were quantified by real-time PCR using TB Green Premix Ex Taq II (Tli RNaseH Plus) (TaKaRa) and the following primer pair ...
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bioRxiv - Bioengineering 2023Quote: ... with each 20 μl of PCR mixture containing 10 μl of TB Green Premix Ex Taq II (Tli RNase H Plus, Takara), 0.4 μl of each PCR forward and reverse primers (10 μM) ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells (live singlet CD19+ CD4− IgDlo CD71+CD38int CD20+ CXCR5+) were sorted using a FACSAria II into 96-well plates containing 2 μL Lysis Buffer (Clontech) supplemented with 1 U μl−1 RNase inhibitor (NEB) ...
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bioRxiv - Genetics 2023Quote: ... and subjected to quantitative real-time polymerase chain reaction with a TB Green Premix Ex Taq II kit (Takara, RR820A). The PCR program consisted of 95°C for 1 minute ...
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bioRxiv - Microbiology 2023Quote: ... and the internal control feline peptidyl prolyl isomerase A (PPIA) was amplified using primers Fe-227S and Fe-204R via SYBR Premix Ex Taq II (Tli RNaseH Plus; Takara). Thermal cycling was performed according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... and axis sites (YBP1, GRR1) (see qPCR Primer Table) using the TB Green Premix Ex Taq II (Tli RNAse H Plus, Takara).