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Citations for Takara Bio :
51 - 100 of 237 citations for Urotensin I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After being treated with DNase I (Takara Bio), the synthesized guide RNA was purified using the RNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Pathology 2020Quote: ... 100 μg/ ml Dnase I (Cat.#2270A, TaKaRa) and 100 μg/ml Rnase A (Cat.#AC118 ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol) containing DNase I (10 U, Takara) and incubated at 37 °C for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was removed using DNase I (TaKara). An RNA-seq transcriptome library was prepared using the TruSeq™ RNA sample preparation Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was eliminated by DNase I (Takara) treatment ...
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bioRxiv - Plant Biology 2022Quote: ... without DNA contamination upon DNase I treatment (Takara). After quality control (OD26/280=1.8-2.2 ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was removed with DNase I (Takara). cDNA was then reverse transcribed via SuperScript VILO cDNA Synthesis Kit (Invitrogen Life Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... Extracted RNA was treated with DNase I (Takara) to remove contaminating DNA ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was removed using DNase I (TaKara). RNA-seq transcriptome libraries were prepared using the TruSeq RNA sample preparation kit from Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The cloned fragment was inserted into the plasmid pPICZαA containing the AOX1 promoter by replacing the fragment between the EcoR I site and Xba I site using an In-Fusion HD cloning kit (Clontech, Japan). To express recombinant proteins with a fluorescent tag ...
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bioRxiv - Cell Biology 2023Quote: ... manuscript in preparation) The genetic sequence for H2B-mScarlet-I-DAAO and TOM20-mScarlet-I-DAAO was introduced by infusion cloning (Takara Bio) into a lentiviral backbone under control of a human EF-1α promoter and containing a puromycin selection cassette ...
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bioRxiv - Molecular Biology 2019Quote: ... The amplified fragment was cloned into the EcoR I and BamH I restriction enzyme sites of pMD18-T vector (TaKaRa, Kusatsu, Japan) and verified by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... The pCold-I vector (Takara Bio Inc., Shiga, Japan) was used to construct an expression vector to produce WT and mutant versions of recombinant GyrA and GyrB proteins from Mul shinshuense and Agy99 strains ...
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bioRxiv - Zoology 2021Quote: ... Genomic DNA was subsequently removed using DNase I (Takara). Meta-transcriptome library preparation of each pool was carried out using the TruSeq™ Stranded Total RNA Sample Preparation Kit from Illumina (San Diego ...
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bioRxiv - Microbiology 2021Quote: ... and genomic DNA was removed using DNase I (TaKaRa). The RNA-seq transcriptome library was prepared following the TruSeqTM RNA sample preparation kit from Illumina (San Diego ...
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bioRxiv - Neuroscience 2020Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent ...
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bioRxiv - Microbiology 2021Quote: ... Isolated RNA was then treated with DNase I (TaKaRa) to degrade contaminating gDNA ...
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bioRxiv - Genomics 2022Quote: ... and treated with DNase I (Cat# 2270A, Takara Bio). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Cat# 18080400 ...
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bioRxiv - Molecular Biology 2020Quote: ... isolated total RNA was treated with DNase I (Takara) for 0.5–1 hr at 37°C and re-purified using Acid-Phenol/Chloroform (Ambion) ...
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bioRxiv - Microbiology 2019Quote: ... After treatment with DNase I (Takara Bio, Shiga, Japan), RNA was cleaned-up with NucleoSpin RNA kit (Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: ... All RNA was digested with DNase I (Takara, Japan). 1 ug total RNA and PrimeScript RT Master Mix (Takara ...
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bioRxiv - Microbiology 2021Quote: ... TE buffer with SYBR Green I (TaKaRa-Bio, Inc.) was mounted on thin sections and covered with cover glasses ...
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bioRxiv - Genomics 2021Quote: ... with Quantitect SYBR I Green (TaKaRa Biotechnology [Dalian] Co.Ltd) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The remaining plasmids were treated with DNase I (Takara), and the transcribed RNAs were purified using the RNeasy Mini kit (Qiagen).
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bioRxiv - Plant Biology 2022Quote: ... 5 units of recombinant DNase I (TaKaRa, Kyoto, Japan), 200 units of RevertAid™ reverse transcriptase (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was removed using DNase I (TaKaRa). The transcriptome libraries were constructed by using the TruSeqTM RNA sample preparation Kit from Illumina (San Diego ...
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bioRxiv - Plant Biology 2023Quote: ... The restriction enzyme Dpn I (Takara-Bio, Kusatsu, Japan) was added to the PCR product and the solution was incubated at 37°C for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... each RNA sample was treated with DNase I (TAKARA) at 37
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bioRxiv - Plant Biology 2024Quote: ... DNA polymerase I was purchased from Takara (Dalian, China). Key instruments and equipment include the PDS-1000 desktop gene gun (Bio Rad ...
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bioRxiv - Pathology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined using Agilent 2100 BioAnalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA was removed using DNase I (Takara). Then ...
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bioRxiv - Biochemistry 2020Quote: ... Synthesized gene fragments went through cloning process after overnight digestion with restriction enzymes (Nde I, Xho I) at 37 °C and ligation (T4 DNA Ligase, Takara Bio, Shiga, Japan) into pET21 vector (Novagen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then treated with RNase-free DNase I (Takara, Japan). The purity and quantity of the extracted RNA were measured using a NanoVue spectrophotometer (GE Healthcare Bio-Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 U/mL DNase I (2212, Takara, Dalian, China) at 37 °C for 10 min with intermittent agitation every 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Genomic DNA was removed using DNase I (TaKaRa, Dalian, China). RNA degradation and contamination were monitored using 1% agarose gels ...
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bioRxiv - Microbiology 2021Quote: ... After pretreatment with RNase-free DNase I (TaKaRa, Dalian, China), total RNA (1 μg ...
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bioRxiv - Plant Biology 2020Quote: ... The extracted RNA was treated with Recombinant DNase I (TaKaRa), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... We inserted ptrA for pyrithiamine resistance from pPTR I (Takara) or hygB for hygromycin resistance from pBC-hygro (Creative Biogene ...
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bioRxiv - Microbiology 2020Quote: ... and is a pHEK293 UltraExpression I vector (pUE1-TSP) (Clontech) which encodes an N-terminal Twin-Strep tag and the coding region for ORF68 including its native start codon ...
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bioRxiv - Microbiology 2022Quote: ... After degradation of DNA using recombinant DNase I (Takara Bio), an equal quantity of total RNA (500 ng ...
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bioRxiv - Plant Biology 2022Quote: ... and then treated with RNase-free DNase I (Takara 2270B) to remove the potential DNA contamination ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of DNase I (15 K units, TaKaRa Bio), 0.2 U/μL of ribonuclease inhibitor (porcine liver ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was then removed using DNase I (Takara, Japan). CircRNA libraries were constructed using the TruSeqTM Stranded Total RNA Sample Preparation Kit from Illumina (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... the RNA samples were treated with recombinant DNase I (TaKaRa). The cDNA was amplified using SYBR Premix Ex Taq II (Tli RNase H Plus ...
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bioRxiv - Microbiology 2024Quote: ... the RNA samples were treated with recombinant DNase I (TaKaRa). The cDNA was amplified using Premix Ex Taq (Probe qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... and EeSGL1 were inserted into pCold I (Takara, Shiga, Japan) to fuse a His6-tag at the N-terminus ...
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara, Dalian). Only high quality RNA samples (OD260/280 = 1.8∼2.2 ...
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bioRxiv - Immunology 2024Quote: ... The full-length CD177 cDNA inserts were sub-cloned into pBMN-I-EGFP retroviral vector digested with Bam HI and EcoR I restriction enzymes using InFusion HD Cloning Kit (Takara Bio USA, Mountain View, Ca) according to the product manual ...
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bioRxiv - Cell Biology 2020Quote: ... and genomic DNA was removed using rDNase I RNase-free (Takara). RNA quality was verified by both ND-2000 (NanoDrop ...