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Citations for Takara Bio :
1 - 50 of 243 citations for Triaryl Phosphate Isopropylated Grade 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... using calcium-phosphate (Takara). Media was changed at 24 hours and samples were collected 72 hours post-transfection.
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bioRxiv - Neuroscience 2020Quote: ... a Calcium Phosphate Transfection kit (Clontech) was used to transfect cells with a mixture of OXTR-iTango2 DNA plasmid vectors with a secreted alkaline phosphatase (SEAP ...
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bioRxiv - Bioengineering 2022Quote: ... were transiently transfected using calcium phosphate (Takara) and retrovirus-containing supernatants were collected 2 days later ...
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bioRxiv - Cell Biology 2020Quote: ... pre-equilibrated with phosphate-buffered saline (PBS; TAKARA). After the addition of the reaction mixture (100 μL ...
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bioRxiv - Cell Biology 2022Quote: ... and preserved in phosphate-buffered saline (PBS; TaKaRa) at 4 °C until use ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in phosphate-buffered saline (PBS; #T9181, Takara) was placed onto the gap created by removing flagellum ...
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bioRxiv - Neuroscience 2024Quote: ... using a calcium phosphate transfection kit (Takara #631312). The cell culture supernatant was collected at 48 hours post-transfection using centrifugation at low speed (1500×g ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293T cells were transfected with calcium phosphate (Clontech) in T75 flasks using a total of 30 μg plasmid DNA ...
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bioRxiv - Biophysics 2021Quote: ... using the calcium phosphate method (Takara Bio, Shiga, Japan). All animal experiments complied with the protocols approved by the Institutional Animal Care and Use Committee of Tohoku University (2016EgA-003 ...
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bioRxiv - Developmental Biology 2021Quote: ... pupal wings were fixed in PBS (phosphate buffered saline, Takara Bio) with 4% paraformaldehyde (PFA ...
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bioRxiv - Synthetic Biology 2023Quote: ... in 1x Phosphate-buffered saline (PBS) (Takara #T9181 or Gibco #70011044) was added to each well such that the liquid could cover the entire surface and be aspirated after one hour at 37°C before plating cells ...
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bioRxiv - Cell Biology 2020Quote: ... HBO can be cryopreserved by using STEM-CELLBANKER GMP grade (TaKaRa Bio).
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bioRxiv - Developmental Biology 2023Quote: ... The constructed plasmids then were purified using nucleospin plasmid-transfection grade (TAKARA) and transfected into piezo1-deficient N2A cells (Sugisawa et al. ...
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bioRxiv - Immunology 2021Quote: ... pMD2.G and the CAR-expression plasmid by calcium phosphate transfection (Clontech). At 16 hrs post-transfection cells were washed with PBS and incubated in complete DMEM media containing 6 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... was dissolved in phosphate buffered saline (PBS) (T900, Takara BIO Inc, Japan)13 ...
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bioRxiv - Genomics 2021Quote: ... by calcium phosphate transfection with CalPhos™ Mammalian Transfection Kit (Takara, 631312) following the manufacturer’s protocol into HEK293T cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were collected and suspended into phosphate-buffered saline (PBS) (Takara, T900) containing 20 mM imidazole (Nacalai Tesque ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified and purified by Nucleospin plasmid TF grade (Takara bio, Shiga, Japan). All plasmid sequences were verified using Sanger sequencing (3100 Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: Primers (PAGE grade, fasmac, Appendix Table S4) were labelled with 32P-γ-ATP by T4PNK (Takara), followed by filtering through MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... and the respective retroviral vector (pWPI) using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Molecular Biology 2021Quote: ... as internal control by the calcium phosphate transfection method according to the manufacturer’s protocol (Takara). Raji cells (3×106cells ...
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bioRxiv - Cell Biology 2022Quote: ... the cultured neurons were transfected using the High-Efficiency Ca2+ Phosphate Transfection Kit (Takara Bio). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... 661W cells were transfected with the calcium phosphate technique or the XfectTM transfection reagent (Takara). MEF cells were transfected using TurboFectTM Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized with isoflurane and perfused transcardially with 1X Phosphate Buffered Saline (PBS) (Takara) and 4% Paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized with isoflurane and perfused intracardially with 1X Phosphate Buffered Saline (PBS) (Takara, Japan) and 4% Paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized with isoflurane and perfused intracardially with 1X Phosphate Buffered Saline (PBS) (Takara, Japan) and 4% Paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2020Quote: LNA/DNA probes (PAGE grade, fasmac, Table SX) were labelled with 32P-γ-ATP by means T4PNK (Takara), followed to filter by MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (5′-GCACCGTCAAGGCTGAGAAC-3′ and 5′-TGGTGAAGACGCCAGTGGA-3′; Takara Bio Inc., Shiga, Japan) were used for RT-PCR.
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bioRxiv - Neuroscience 2020Quote: ... in a 100 mm dish using a calcium phosphate transfection kit (CalPhos Mammalian Transfection kit, Clontech, 631312). Two days post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 2019) and 10 fmol JF646-LANA (see below) in 0.5 nL phosphate-buffered saline (PBS, Takara, T900) containing 0.05% phenol red (SIGMA ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were transfected at 11 days in vitro (DIV 11) using Calphos calcium phosphate transfection reagent (Takara) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and the psPAX2 (6.75 μg) vectors by the calcium phosphate transfection method according to the manufacturer’s protocol (Takara). 72 hours post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... male genitalia were dissected in phosphate-buffered saline (PBS: Takara Bio Inc., #T900; pH 7.4 at 25°C), kept in 50% VECTASHIELD mounting medium (Vector Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... and the psPAX2 (6.75 μg) packaging vectors by the calcium phosphate transfection method according to the manufacturer’s protocol (TaKaRa; Calphos). 72h post-transfection ...
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bioRxiv - Pathology 2022Quote: ... mouse brains were extracted after cardiac perfusion with cold phosphate buffered saline (PBS) and homogenized by RNAiso Plus (TAKARA) and homemade RIPA buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into human embryonic kidney (HEK) 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 hours after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters prior to transduction of cancer cell lines using centrifugation at 1000 g for 2 hours in the presence of 8 μg/mL of polybrene (Santa Cruz Biotechnology) ...
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bioRxiv - Biophysics 2024Quote: ... the solution of carboxylated beads was diluted into 400 µL of 1× Phosphate-Buffered Saline (PBS) (Takara Bio, T900) containing 100 mg/mL PLL-g-PEG (SuSoS ...
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bioRxiv - Bioengineering 2024Quote: ... The viral supernatant was then concentrated 100-fold into 1X phosphate-buffered saline (PBS) using LentiX Concentrator (Takara Bio), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Individual lentiCRISPRv2 vectors were introduced along with packaging vectors into HEK 293T cells via calcium phosphate transfection according to the manufacturer’s protocol (Clontech). Lentivirus was harvested at 48 and 72 h after transfection in RPMI media supplemented with 10% FBS and filtered with 45 μm filters before transduction of target cancer cell lines using centrifugation at 1000g for 2 hours in the presence of 8 mg/mL of polybrene (Santa Cruz Biotechnology) ...
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bioRxiv - Cancer Biology 2020Quote: ... primary lymphocytes were stimulated with IL-2 and anti-CD3 and retrovirally transduced with the anti-NY-ESO-1 TCR from clinical grade retroviral supernatants via RetroNectin (Takara Bio). Cells were expanded by a rapid expansion protocol involving soluble OKT3 ...
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bioRxiv - Neuroscience 2020Quote: ... 4 U RNAse inhibitor (Takara, 2313A), 10mM dNTPs (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... retroviral packaging construct pTG5349 and a reporter pCCLSIN.cPPT.hPGK.GFP.WPRE into HEK 293T cells by calcium-phosphate method (Calphos Mammalian Transfection kit, Clontech as per manufacturer’s instructions). Cells were seeded on 60×15mm dish a day before transfection to achieve 70 – 80% confluency ...
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bioRxiv - Cell Biology 2021Quote: Transfection of Halo-tagged AnkB440 plasmids were conducted in HEK293T cells grown in 10 cm culture plates using the calcium phosphate transfection kit (Takara) and 8 µg of plasmid ...
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bioRxiv - Immunology 2022Quote: ... or the expression vector LentiCRISPRv2 (coding for guideRNA) in a 3:1:3 ratio for 8 hours using calcium phosphate transfection (CalPhos Mammalian Transfection Kit, Takara Bio Europe ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiviral constructs pCMV-VSV-G and pCMV-dR8.2 dvpr 98 were transfected in HEK293T cells by the calcium-phosphate method (CalPhos Mammalian Transfection Kit, Takara, 631312) for virus production ...
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bioRxiv - Neuroscience 2024Quote: ... pLV.shRegγ) along with the helper vectors psPAX2 and pMD2.G using the Calcium Phosphate Mammalian Transfection Kit (631312-Takara bio). Lentivirus-containing supernatants were collected 48 hours post-transfection and used to infect target cells in the presence of 10 mg/ml polybrene ...
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bioRxiv - Neuroscience 2022Quote: AAVs were packaged via calcium phosphate transfection of HEK293 cells as detailed in (McClure et al 2011) and purified using an AAVpro purification kit (Takara Bio).
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bioRxiv - Biochemistry 2022Quote: ... cells were transfected with plasmids expressing either SARS-COV-2 S protein WT or mutants (E1182K, L1193G, E1182K/L1193G, L1182K/L1186G/L1193G) by using Calcium phosphate transfection kit (Takara-Bio). 24 h post transfection ...
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bioRxiv - Cancer Biology 2023Quote: Production of vesicular stomatitis virus (VSV-G) pseudotyped lentivirus was performed by calcium phosphate transfection of Lenti-XTM 293T cells (TaKaRa Clontech, 632180) with CROP-mCherry and helper plasmids pMD2.G and psPAX2 (Addgene plasmids 12259 and 12260) ...
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bioRxiv - Immunology 2024Quote: ... containing 0.5 ml of a 1:1 solution of phosphate-buffered saline (PBS):FBS supplemented with ribonuclease inhibitor (1:100; Takara Bio). For some samples ...