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Citations for Takara Bio :
351 - 400 of 2122 citations for Tonsoku Like DNA Repair Protein TONSL Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: For PCR reactions PrimeSTAR HS DNA Polymerase (Clontech), Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... the TaKaRa DNA Ligation Kit LONG (TaKaRa Bio) was used along with NEB Stable Competent E ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5 μL of SeqAmp DNA polymerase (Takara Bio), and 1.95 μL of DDW ...
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bioRxiv - Developmental Biology 2023Quote: ... and amplified with PrimeSTAR GXL DNA Polymerase (TaKaRa) or with SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the PrimeSTAR Max DNA Polymerase (Takara Bio Inc.) was used according to the manufacturer’s recommendation with a 25 µl reaction volume ...
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bioRxiv - Microbiology 2023Quote: ... PrimeSTAR Max DNA Polymerase was obtained from TaKaRa. The DNA purification kit was obtained from Axygen ...
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bioRxiv - Microbiology 2023Quote: ... and ligated with a DNA ligation kit (Takara). The constructs expressing GFP-BDI-bK6A and single amino acid substitutions in the SLiM sequence were generated by PCR-based site-directed mutagenesis using oligonucleotides and templates listed in Table S1 and inserted into the SalI site of pHE-C or phsp-GFP-Halo as described above ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was removed using DNase I (TaKara). RNA-seq transcriptome libraries were prepared using the TruSeq RNA sample preparation kit from Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... We used the GXL HiFi DNA polymerase (Takara) for PCR amplification ...
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bioRxiv - Synthetic Biology 2023Quote: ... PrimeSTAR HS DNA Polymerase was purchased from Takara Bio (Kusatsu ...
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bioRxiv - Genetics 2023Quote: ... and DNA Unique Dual Index Kit (Takara, R400666). Paired-end sequencing of ChIP-seq libraries was performed (150 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... with Takara Ex Premier DNA Polymerase (Takara Bio), are reported in Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA size marker ladders were from Takara. TRIzol reagent from Thermo Fisher Scientific was used for RNA isolation ...
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bioRxiv - Microbiology 2024Quote: ... DNA was removed using DNaseI (Takara Bio Inc.). Reverse transcription of RNA was performed using PrimeScriptTM reagent Kit (Perfect Real Time ...
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bioRxiv - Microbiology 2023Quote: ... with PrimeStar Taq DNA Polymerase (Takara, cat#R045A). Concentrations were measured using the Qubit dsDNA HS (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of PrimeSTAR GXL DNA polymerase (Takara), 200 uM of each dNTP ...
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR fragment was prepared with a normal PCR protocol using KOD-Plus-Neo DNA polymerase (KOD-401, Toyobo, Japan) or ExTaq DNA Polymerase (RR001, Takara, Japan), both of which had 3’-5’ exonuclease activities.
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bioRxiv - Genomics 2019Quote: ... We additionally PCR amplified the region from genomic DNA for sample NA12878 with 12 copies of AC (NIGMS Human Genetic Repository, Coriell) using PrimeSTAR max DNA Polymerase (Clontech R045B) and primers RFT1eSTR_F and RFT1eSTR_R (Supplementary Table 8 ...
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bioRxiv - Microbiology 2019Quote: ... discoideum were amplified from cDNA (Robinson and Spudich 2000) or genomic DNA of the Ax2 strain using PrimeStar Max DNA polymerase (Takara Bio) and the designed primers ...
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bioRxiv - Neuroscience 2020Quote: ... Mice produced were genotyped by genomic DNA purified from the tail and amplified with LA Taq HS DNA polymerase (Takara, RR042) with the upstream primer (5’-AGAATGGAGGCTGGCCTAAC-3’ ...
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bioRxiv - Molecular Biology 2020Quote: Specific DNA sequences were PCR amplified using gene specific primers and cloned into vectors using PrimeSTAR HS DNA polymerase (#R010A, Takara-bio) and T4 DNA ligase (#M0202S ...
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bioRxiv - Genomics 2022Quote: ... colonies were picked into 96-well plates and a small proportion of cells from each colony were used for DNA extraction using Quick Extract solution (Epicenter) and direct PCR with Prime STAR® GXL DNA Polymerase (Clontech). PCR amplicons were sequenced by Sanger to verify the deletion (SFigure 17).
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bioRxiv - Molecular Biology 2021Quote: ... One ng of DNA was used for real-time qPCR analysis using Premix Ex Taq DNA polymerase (Takara, cat. no. RR039W) on ABI Fast 7500 (Applied Biosystems® ...
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bioRxiv - Genetics 2020Quote: ... The three fragments were all amplified from the genomic DNA of Ki(th-P2A-EGFP) zebrafish by using the PrimeSTAR HS DNA polymerase (Takara, Japan). The kdrl-loxP-exon12-frt-SM-frt-loxP or tcf3a-loxP-exon4-frt-SM-frt-loxP replacement donor were constructed by ligating four fragments (a left arm ...
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bioRxiv - Microbiology 2021Quote: ... The genome DNA was used as template for PCR amplification with the barcoded primers and Tks Gflex DNA Polymerase (Takara, USA). The hypervariable V3-V4 regions of bacterial 16S rRNA were amplified using universal primers 343F 5’-TACGGRAGGCAGCAG-3’ and 798R 5’-AGGGTATCTAATCCT-3’ (Nossa et al. ...
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bioRxiv - Physiology 2021Quote: ChIP-seq libraries were prepared on 5 ng of DNA from ChIP and input samples using the SMARTer ThruPlex DNA-seq kit along with DNA HT Dual Index Kit (both from Takara Bio) according the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: The proS gene was amplified from 50 ng population genomic DNA in a 25 μL PCR reaction using the high-fidelity polymerase PrimeSTAR Max DNA Polymerase (Takara Bio) and primers 134 and 138 (Supplementary Table 7) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA and DNA were extracted from the larvae using a NucleoSpin RNA XS and a NucleoSpin RNA/DNA Buffer Set (Takara Bio). If individuals were homozygous at two CSD loci (male genotype ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from all the MBS and soil samples using the NucleoSpin Soil DNA extraction kit (Takara Bio, USA), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... three primer sets were designed with overlapping sections,100ng of Genomic DNA was used per PCR reaction with primeSTAR GXL DNA polymerase (Takara Bio) using a touchdown PCR for the first 10 cycles from 72 to 60 followed by 35-40 cycles at the proper annealing temperature (Tm −2°C ...
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bioRxiv - Cell Biology 2019Quote: ... SMAD2 #3122 and SMAD3 #9523 antibodies (Cell Signalling Technology) and anti-FLAG antibody (Clontech). Horseradish peroxidase-conjugated secondary antibodies and anti-rabbit and anti-mouse antibodies were acquired from Molecular Probes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The following antibodies were used: Anti-GFP antibody (JL-8 from Takara, Shiga, Japan), anti-Flag antibody (M2 from Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... or anti-GFP antibody (Clontech) for 1 hour at room temperature followed by a second incubation with HRP-conjugated secondary antibody (Santa Cruz Biotechnology) ...
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bioRxiv - Microbiology 2024Quote: ... Monoclonal Antibody (JL-8) (Clontech). Anti-mouse IgG was used as a secondary antibody.
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with TALON beads (Clontech) pre-equilibrated with purification buffer at RT for 1.5 h with overhead rotation ...
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bioRxiv - Plant Biology 2020Quote: ... the proteins were purified by TALON Metal Affinity Resin (Clontech) and filtered with Amicon Ultra 0.5ml (30K ...
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bioRxiv - Synthetic Biology 2021Quote: ... concentrated proteins were determined those concentrations with Bradford (Takara Bio) or BCA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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bioRxiv - Cell Biology 2020Quote: ... The solubilized protein was purified by Talon-resin (Clontech/Takara) using the hexa-histidine-tag fused at the N-terminus of the protein ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was purified on a His60 Ni Superflow column (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... which encodes the chaperone protein tag (TaKaRa Bio, Shiga, Japan), following the manufacturer protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cyan fluorescent protein (CFP) expression plasmid was pECFP-C1 (Clontech). Plasmids pCAGGS-CD4-Myc (Addgene ...
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bioRxiv - Genomics 2023Quote: ... and tdTomato fluorescent protein using in-Fusion cloning (Takara Bio). The 5-plasmid system includes a packaging vector (pHAGE-H2B-NanoLuc-T2A-tdTomato) ...
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bioRxiv - Biochemistry 2023Quote: ... protein complexes were purified from by Ni2+-NTA (Takara Bio) affinity chromatography (Dsl1:Qb:Qc and His7-Tip20:Sec20 ...
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bioRxiv - Molecular Biology 2023Quote: ... sfGFP-tagged proteins were visualized with monoclonal anti-GFP (Takara). Anti-Sty1 polyclonal antibody (Jara et al ...
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bioRxiv - Cell Biology 2024Quote: ... and protein concentration was estimated by BCA Kit (Takara-#T9300A). For the assay ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA samples from the patients’s paired tumor tissues and WBCs were used to prepare DNA libraries for DNA sequencing with the ThruPLEX Tag-seq Kit (Takara Bio, USA). The libraries were then pooled and hybridized with pre-designed probes for 95 targeted genes (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 µl used as a source of genomic DNA in 20 µl PCR reactions with PrimeStar Hot Start DNA polymerase (Takara Bio, R010A). Amplified DNA was subcloned with StrataClone Blunt PCR cloning kit (Agilent Technologies ...