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Citations for Takara Bio :
1 - 50 of 939 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Samples showing the correct size band for heavy and light chain amplification in the respective wells were subsequently cloned into pAb20-hCHIgG1 (Synbio; for heavy chain) and pAb20-hCK (Synbio; for light chains) through in-fusion cloning (Takara). The ligated product was transformed to DH5-α competent cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Coding regions of the heavy-chain variable domains were amplified using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan) with two PAGE-purified primers (CALL001 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Polymerase chain reaction (PCR) fragments were generated using PrimeSTAR Max DNA Polymerase (TaKaRa). The method for plasmid construction is described below.
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bioRxiv - Microbiology 2022Quote: Sequence of the human variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma ...
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bioRxiv - Microbiology 2023Quote: Sequence of the mouse variable heavy and kappa chains were obtained by using SMARTer 5’ RACE technology (Takara Bio, USA) adapted for antibodies to amplify the variable genes from heavy and kappa chains for each hybridoma based on isotype ...
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bioRxiv - Immunology 2022Quote: ... RetroNectin® Recombinant Human Fibronectin Fragment (Takara Bio, Cat#T100A), Recombinant Murine Flt3-Ligand (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... and used to generate Illumina-ready heavy and light chain sequencing libraries using the SMARTer Mouse BCR IgG H/K/L Profiling Kit (Takara, Cat# 634422). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... downstream of the Myh6 (α- myosin heavy chain) promoter and upstream of a human growth hormone polyadenylation signal using In-Fusion HD (Takara Bio cat# 011614). The final transgenic targeting construct was verified by DNA sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus transduction was performed using RetroNectin® Recombinant Human Fibronectin Fragment (Takara, cat.no. T100A/B) according to the manufacturer’s instructions (with centrifugation) ...
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bioRxiv - Biophysics 2023Quote: Plasmids used in this study (Supplementary Table S1) were assembled by Polymerase Chain Reactions (PCR) amplifying insert and vector DNA fragments followed by In-Fusion cloning (Takara Biosciences, In-Fusion HD Cloning Kit). Oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2022Quote: Clontech SMARTer Polymerase Chain Reaction kit (Takara) was used to produce compatible polyA-transcript cDNA ...
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bioRxiv - Cell Biology 2019Quote: The plasmid pDendra2NatMX was constructed by ligation of the NheI-HpaI Dendra2 fragment from pDendra2-C (Clontech) and the PvuII linearized pAG25 vector (Addgene) ...
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bioRxiv - Microbiology 2020Quote: ... for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio). Next ...
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bioRxiv - Microbiology 2023Quote: ... for 15 min at 37°C in the presence of 80 U of recombinant RNase inhibitor (Takara Bio). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... and the total extracted RNA was reverse-transcribed into cDNA for polymerase chain reaction amplification using the real-time polymerase chain reaction SYBR Green kit (TaKaRa, Guangzhou, China). The reverse transcription steps were as follows ...
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bioRxiv - Microbiology 2023Quote: Polymerase chain reaction (PCR) was conducted using ExTaq (TaKaRa) to amplify the SLST locus ...
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bioRxiv - Molecular Biology 2024Quote: ... Polymerase chain reactions were performed using PrimeSTAR Max (Takara). Synthetic genes for mGreenLantern and mLychee were ordered from Integrated DNA Technologies.
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant RNase inhibitor (Takara) and protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Systems Biology 2023Quote: ... Recombinant DNase I (TaKaRa) was used to digest DNAs ...
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bioRxiv - Plant Biology 2019Quote: 6His-GST-CRK2cyto and 6His-MBP-RBOHD/C constructs for recombinant proteins were generated by using In-Fusion technology (Clontech). The coding regions of CRK2cyto (WT ...
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bioRxiv - Systems Biology 2021Quote: ... for about 60 minutes at 37°C and sorted into lysis buffer (4μl 0.5 U/μL Recombinant RNase Inhibitor (Takara Bio, 2313B), 0.0625% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... GFP CIZ1 C275 was made by ligating the 1 kb C-terminal XhoI fragment (Coverley et al., 2005) into the XhoI site of pEGFP-C2 (Clontech). GFP CIZ1 Δp8 (this study ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding C-terminally His-tagged Gtsf1 or Gtsf1L was amplified by PCR and cloned into pCold vector (Takara) by In-fusion cloning kit (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... and recombinant Cas9 proteins (Clontech) were introduced together into the KhES-1 cells by electroporation (Neon device ...
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bioRxiv - Immunology 2019Quote: ... 1.85U recombinant RNase Inhibitor (Takara), 1.85 μM template-switching oligo was aliquoted to each lysed cell using a Nanodrop II liquid handling system (BioNex ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant Cas9 protein (TAKARA) and the sgRNA were incubated for 10 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... DNA fragments corresponding to MV-C or CH coding sequences were amplified using pTM3-MVSchw or pmCherry (Clontech, Palo Alto, USA) as a template ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA fragments were generated in the reaction with Klenow fragment (Takara-Bio, Japan) and a single primer ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplified fragments were gel purified and used for In-Fusion Multiple Fragment Cloning (Clontech) according to manufacturer’s directions ...
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bioRxiv - Immunology 2021Quote: ... 0.25 μL Recombinant RNase Inhibitor (Clontech), 2 μL Betaine (5 M Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... and Recombinant RNAse i nhibitor (TaKaRa). cDNA was prepared from total RNAs using ReverTra Ace (TOYOBO) ...
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bioRxiv - Neuroscience 2020Quote: ... 1.85 U recombinant RNase Inhibitor (Takara), 1.85 µM template-switching oligo ...
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bioRxiv - Cancer Biology 2020Quote: ... and Recombinant RNase Inhibitor (Takara, Japan) were added into the cell pellets ...
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bioRxiv - Neuroscience 2022Quote: ... 0.25 μL Recombinant RNase Inhibitor (Clontech), 2 μL Betaine (5 M Sigma) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.85 U recombinant RNase Inhibitor (Takara), 1.85 µM template-switching oligo was aliquoted to each lysed cell using a Nanodrop II liquid handling system (BioNex ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... 0.25 μL Recombinant Ribonuclease Inhibitor (Takara), and 10 U/μL Enzscript Moloney-Murine Leukemia Virus Reverse Transcriptase (Enzymatics) ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 U recombinant inhibitor (TaKaRa) for samples containing biological inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 U recombinant inhibitor (TaKaRa) for samples containing biological inhibitor ...
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bioRxiv - Molecular Biology 2020Quote: ... A polymerase chain reaction (PCR) master mix containing ExTaq DNA polymerase (Clontech), ExTaq buffer (Clontech) ...
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bioRxiv - Cell Biology 2021Quote: ... A polymerase chain reaction (PCR) master mix containing ExTaq DNA polymerase (Clontech), ExTaq buffer (Clontech) ...
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bioRxiv - Bioengineering 2023Quote: ... ssDNA fragments were transformed to dsDNA by Klenow fragment DNA polymerase (Takara Bio, Shiga, Japan) with primer 1 ...
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bioRxiv - Zoology 2019Quote: ... Polymerase chain reactions (PCRs) were performed using Tks Gflex™ DNA Polymerase (Takara) and conducted in a T100™ Thermal Cycler (Bio-Rad) ...
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bioRxiv - Bioengineering 2021Quote: Polymerase Chain Reactions (PCR) were conducted using PrimeSTAR GXL DNA Polymerase (Takara Bio) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Polymerase chain reaction (PCR) was performed using PrimeSTAR GXL DNA Polymerase (TaKaRa, R050A), following the recommended Rapid PCR protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Polymerase chain reaction (PCR) was performed using Primer STAR Max DNA Polymerase (Takara) to amplify the target gene ...
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bioRxiv - Microbiology 2020Quote: ... 160 μl recombinant RNase inhibitor (Takara Clonetech), 1.6 ml of 10 mM dNTP (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... and treated with recombinant DNase I (Takara). The treated RNA was purified with an RNeasy Mini kit (Qiagen) ...
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bioRxiv - Bioengineering 2020Quote: ... 160 µL recombinant RNase inhibitor (Takara Clonetech), 1.6 mL of 10 mM dNTP (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... and 1ul recombinant RNase inhibitor (Takara, 2313B) incubated on ice for 5min ...