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Citations for Takara Bio :
1 - 50 of 1323 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and the BrSCR-46 cassette was similarly cloned into the plasmid containing the BrSRK-46 chimera by InFusion cloning (TAKARA Bio). Similarly ...
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bioRxiv - Cell Biology 2020Quote: ... The tD-tomato-N1 vector was obtained from Clontech. The construct coding for a GFP-fusion of the SHANK3 SPN domain has been described previously 14,46 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 (TOYOBO, Osaka, Japan) or the In-Fusion cloning kit (Clontech, Mountain View, CA). To generate pCSIIpuro-Necl5-ScNeo ...
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bioRxiv - Cancer Biology 2021Quote: ... including a puromycin resistance cassette and a tet-responsive element for expression of shRNAs was established according to a publicly available protocol (46) using In-Fusion HD Cloning Kit (Clontech) (Supplementary Table 3) ...
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bioRxiv - Microbiology 2022Quote: ... These two amplified fragments were inserted into the pBh-EPS vector [46] at the PstI site using the In-FusionHD Cloning Kit (Takara). The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then amplified using the Advantage 2 PCR Enzyme System (Takara, Fremont, CA) for 6 cycles ...
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bioRxiv - Bioengineering 2022Quote: The transfer vector pABpaR2pX was constructed by inserting a DsRed2 reporter gene driven by pag promoter(46) (p-pag) into the EcoRV restriction enzyme site of pBacPAK8 (Clontech Laboratories, Inc.). cDNAs encoding HA7 and NA9 were synthesized by GenScript ...
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bioRxiv - Developmental Biology 2024Quote: ... using the same paired read adapter oligonucleotides described by 99 were prepared from the ChIP and Input DNAs on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification was performed using the Advantage 2 Polymerase Mix (Clonetech, now Takara Bio USA, Mountain View, CA) and the following primers ...
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bioRxiv - Genomics 2021Quote: ... Long range PCR was performed using Advantage 2 Polymerase following the manufacturer’s protocol (Clontech Laboratories, Mountain View CA).
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bioRxiv - Biochemistry 2022Quote: ... and ligated into the multiple cloning site 2 (MCS2) of the pTRE3G-BI vector (Clontech, Mountain View, CA, USA), resulting in the construct pTRE3G-BI/GPR83-LgBiT ...
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bioRxiv - Cell Biology 2023Quote: ... Fractionated CD34+ cells from Animals #2 and #3 were cultured overnight on RetroNectin-coated plates (Takara, T100B, Mountain View, CA) in X-VIVOTM 10 (Lonza ...
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bioRxiv - Bioengineering 2020Quote: ... The lysate was loaded onto a prepared column with 2 mL TALON Metal Affinity Resin (Clontech Laboratories, Inc., Mountain View, CA). After being washed twice with 5 column volumes (CV ...
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bioRxiv - Cancer Biology 2024Quote: ... and 2 μg was used to synthesize cDNA using the RNA to cDNA EcoDryTM PreMix (Double Primed) (Takara Bio, CA, USA). Reaction tubes were placed in a thermocycler and subjected to 42°C for 1 h and 70°C for 10 min ...
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bioRxiv - Immunology 2020Quote: Transferrin over-expression or knockdown vectors were constructed and HEK 293T cells (Conservation Genetics CAS Kunming Cell Bank, China) and EcoPack™ 2–293 cells (Clontech, USA) were used to package lentiviruses and retroviruses ...
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bioRxiv - Genetics 2023Quote: ... RNA from 2 retinae of a mouse was extracted using the NucleoSpin® RNA kits (Takara Bio USA, Inc., San Jose, CA). RNA sample concentration and quality was determined with NanoDrop Oneᶜ Microvolume UV-Vis Spectrophotometers (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... to a final concentration of 5 µg/mL and 2 µg/mL doxycycline (631311, Takara Bio USA, Inc., Mountain View, CA, USA) for the first 7 days after seeding ...
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bioRxiv - Cell Biology 2020Quote: ... and Evl (isoform 2) were amplified by PCR from a NIH 3T3 cDNA library and ligated into suitable sites of pEGFP-C1 (Clontech, Palo Alto, CA). VASP cDNA was additionally inserted into the BglII and SalI sites of pmCherry (Addgene ID ...
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bioRxiv - Cell Biology 2022Quote: ... Transformants were selected on synthetic media containing yeast nitrogen base (YNB, Difco) supplemented with 2 % glucose and DropOut supplements lacking uracil (Clontech, Mountain View, CA). Single colonies were used to inoculate 5 mL liquid YNB media supplemented with 2 % glucose and DropOut supplements lacking uracil ...
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bioRxiv - Neuroscience 2023Quote: ... were transiently cotransfected with a 2:1 mixture of Nav1.7-expressing plasmid8 and a pIRES2-ZsGreen bicistronic plasmid (Takara Bio, San Jose, CA) expressing ZsGreen and mouse FHF2B proteins.10 The same pIRES2-ZsGreen plasmid without FHF2 coding sequence served as the control ...
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bioRxiv - Microbiology 2021Quote: ... The knockout construct was developed through In-Fusion using the purified amplicons based on the manufacturer’s instructions with the insert to vector ratio of 2:1 (Takara Bio, Mountain View, CA, USA) and transformed into E ...
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bioRxiv - Bioengineering 2024Quote: ... To synthesize cDNA, total RNA (8 µL, ca. 500 ng) was mixed with 2 µL of PrimeScript™ RT Master Mix (Takara Bio, Kusatsu, Japan) and incubated at 37°C for 15 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 µL of 2 mM dNTPs (Takara #4025), and 0.6 µL of 100% DMSO (NEB #12611P) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) and purified using ProbeQuant G-50 Micro Columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: DNA encoding the PX domain from the Qc SNARE Vam7 (Amino acyl residues 2-123) was amplified by PCR with CloneAMP HiFi PCR premix (Takara Bio USA, Mountain View, CA, USA). The amplified DNA fragment was cloned into BamHI and SalI digested pGST parallel1 vector (Sheffield et al.,1999 ...
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bioRxiv - Neuroscience 2021Quote: ... pDsRed-ER (Takara Bio, CA), RUSH-GPI-mApple ...
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bioRxiv - Neuroscience 2020Quote: ... pDsRed-ER (Takara Bio, CA), RUSH-GPI-mApple ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFP (Clontech, Mountain View, CA), pEGFP2 and pEGFP3 ((Pack et al. ...
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bioRxiv - Immunology 2020Quote: ... pDsRed-mito was purchased from Clontech (Takara, Mountain View, CA, USA). Each construct was verified by sequencing.
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bioRxiv - Neuroscience 2023Quote: ... and eGFP (Clontech. CA, USA) in tsA201 cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 (Takara, #6045) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... version 2 (Takara). Fastq files were generated from bcl files using BCL2FASTQ v2.17.1.14 with default parameters ...
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bioRxiv - Neuroscience 2022Quote: ... 2 (Takara Bio).
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bioRxiv - Molecular Biology 2023Quote: ... 2 (Takara Bio).
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bioRxiv - Microbiology 2021Quote: ... or Stellar (TaKaRa, Mountain View, CA) cells and purified using QIAprep spin and midi kits (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... GP2-293 cell line (Takara, CA) was used as the viral packing cell line for plasmid encoding CAR construct ...
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bioRxiv - Cell Biology 2023Quote: ... or pEGFPC1 (Clontech, Mountain View, CA). Mouse Fcho2 was cloned into pCMV-Tag3B-myc or pmCherry-C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Inc (Takara Bio, San Jose, CA). Converted cDNA products were next column purified and subsequently amplified using universal primers designed relative to an internal consensus sequence derived from analysis of 800 patient samples ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 (Takara, Shiga, Japan) and 0.32 µM of each primer according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... from pTRE-Tight (Clontech, Mountain View, CA) to generate CSII-TRE-Tight-RfA ...
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bioRxiv - Cell Biology 2020Quote: ... WT ZAP-70 or C564R ZAP-70 was cloned into the pmCherry-C1 vector from Clontech (now Takara Bio; 632524; Mountain View, CA) using the NEBuilder high-fidelity DNA assembly cloning (NEB ...
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bioRxiv - Genomics 2021Quote: ... (Takara Bio USA, Inc. Mountain View, CA). Libraries were sequenced in-house using a NextSeq 500 High- Throughput Sequencing System (Illumina San Diego ...
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bioRxiv - Genomics 2020Quote: ... (Takara Bio USA, Inc, Mountain View, CA). Libraries were sequenced in-house using a NextSeq 500 High-Throughput Sequencing System (Illumina ...
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bioRxiv - Genomics 2020Quote: ... and TRH (Takara Bio USA, CA, USA). The chip was sealed with Microseal A film and recentrifuged as above ...
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bioRxiv - Neuroscience 2021Quote: ... The ribo-depletion kit (RiboGone, Takara, CA) and SMARTer Stranded total RNA sample prep kit were used to remove excess ribosomal RNA and synthesize cDNA library ...
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bioRxiv - Neuroscience 2022Quote: ... pEGFP-C3 (Clontech, Mountain View, CA, USA), and pFLAG-CMV2 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... pVSV-G (631530, Clontech, Mountain View, CA) and psPAX2 ...