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Citations for Takara Bio :
101 - 150 of 1728 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... non-tissue culture treated 48 well plates were coated with RetroNectin (Clontech) according to the manufacturer’s recommendations overnight at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Routine passaging was performed in N2B27 basal culture medium (NDIFF, Takara #y40002), supplemented with 1 μM PD0325901 and 3 μM CHIR99021 (both from Axon Medchem) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary hippocampal cultures were transfected using CalPhos mammalian transfection kit (Clontech, 631312) as previously (Jiang & Chen ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were tested regularly for mycoplasma (6601; TaKaRa PCR Mycoplasma Detection Set).
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bioRxiv - Cell Biology 2022Quote: ... The virus-containing media were harvested and concentrated with Lenti-X concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... after which media was collected and virus was concentrated with Lenti-X (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Transformants were selected on Synthetic Defined (SD) media lacking Leucine and Tryptophane (Clontech). Three individual clones were grown ON in liquid cultures (lacking Leucine and Tryptophane ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing media was harvested for virus isolation using Lenti-X concentrator (Takara) in lieu of ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... media was removed and concentrated using lenti-X concentrator solution (Takara Bio #631232). Concentrated viral particles were distributed into single-use aliquots and stored at −80°C until use ...
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bioRxiv - Plant Biology 2021Quote: ... Culture mediums SD/-Leu/-Trp and SD/-Ade/-His/-Leu/-Trp (Clontech, USA) with or without X-α-gal were used to select the positive transformants.
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bioRxiv - Microbiology 2020Quote: ... Monoclonal antibodies were purified from hybridoma culture supernatants using Thiophilic-Superflow Resin (Clontech) or Ab-Capcher MAG2 (ProteNova ...
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bioRxiv - Genomics 2023Quote: ... Parasite cultures were split into two flasks and 250 nM rapalog (AP21967, Clontech) was added to one of the two flasks to induce excision of endogenous EXP1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Clarified lysate was mixed with ∼0.5 ml/L of culture cobalt resin (TaKaRa) for one hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Untreated tissue culture plates were coated with 25mg/ml Retronectin (Takara Bio #T100B) overnight at 4°C to be used for retroviral transduction ...
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bioRxiv - Plant Biology 2021Quote: ... SD growth medium w/o Ade−His−Leu−Trp− (Himedia) and supplements media (Clontech) were used for the Y2H experiment.
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bioRxiv - Cell Biology 2021Quote: ... the media was incubated with ⅓ volume of Lenti-X Concentrator (Takara, Cat No. 631231) at 4°C for 1 hour ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... or media with Tet System Approved FBS (Takara Bio cat# 631107; Mountain View, USA) with and without doxycycline hyclate for 72 h and lysed in RIPA buffer (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... culture supernatants were collected and virus concentrated 10x overnight with Lenti-X concentrator (Clontech) according to the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... culture medium was collected filtered and concentrated using the Lenti-X™ Concentrator (Takara). Viral particles were suspended in fresh DMEM medium and stored at - 80°C ...
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bioRxiv - Plant Biology 2021Quote: ... Transformants were selected by cultivation for 2 days on minimal synthetic defined (SD) media (Clontech) lacking Leu (pACT2-GW ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Genetics 2022Quote: ... Colonies were grown on media lacking histidine and leucine (DO Supplement -His/-Leu, Takara Bio) to select for the presence of both vectors ...
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bioRxiv - Neuroscience 2023Quote: ... Lentivirus was harvested from the media at 72 hours concentrated by LentiX concentrator (Takara # 631232) per the protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara) and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...
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bioRxiv - Microbiology 2023Quote: ... the media was replaced and supplemented with 1 μg/mL aTc (Clontech catalog number 631310). After 24 h induction ...
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bioRxiv - Physiology 2023Quote: ... Harvested conditioned media were incubated with Talon Metal Affinity resin (Clontech, Silicon Valley, CA, USA) at 4 °C overnight ...
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bioRxiv - Immunology 2019Quote: ... non-tissue culture treated 24-well plates were coated with 50 μg/mL Retronectin (Takara) overnight at 4 °C and washed once with PBS ...
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bioRxiv - Biophysics 2020Quote: ... and allowed to bind to cobalt resin (Talon, Clontech; 1 mL bed volume/L culture). The column was washed sequentially with cobalt wash buffer (CoWB ...
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bioRxiv - Immunology 2022Quote: ... lentiviruses in the culture supernatants were concentrated by using lenti-X concentrator (Takara bio, 631231). iBMDMs were seeded into 6-well plates overnight at a density of 1×105 per well ...
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bioRxiv - Immunology 2023Quote: ... the non-tissue culture 24-well plates were coated with 7 μg/ml RetroNectin (TAKARA) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus in the culture medium was concentrated using a lentivirus concentrator (Clontech Labs 3P #631231). One-third of the concentrated virus was used to infect 2.5 x 105 293T cells in the presence of 0.8 mg/ml polybrene ...
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bioRxiv - Immunology 2024Quote: ... Virus was plated on non-tissue culture treated 24-well plates precoated with retronectin (TaKaRa) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatant was recovered and mixed with a third volume of LentiX Concentrator (Takara Bio), incubated at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ... and were routinely cultured on gelatin-coated plates in t2i/L media: NDiff (N2B27) (Takara #y40002) supplemented with titrated 2i (0.2μM PD0325901 ...
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bioRxiv - Developmental Biology 2020Quote: ... We collected viral media at 24 and 48 hours and concentrated using Lenti-X Concentrator (Clontech) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral media was then kept at 4°C before 100x concentration using LentiX concentrator (Takara #631232) and resuspension of virus in organoid seeding media ...
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bioRxiv - Genomics 2021Quote: ... The dCas9KRAB lentivirus was concentrated 20X the initial media volume using Lenti-X concentrator (Clontech, 631232), following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Co-transformants were selected by cultivation for 2 days on minimal synthetic defined (SD) media (Clontech) lacking Leu (pACT2-GW ...
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bioRxiv - Neuroscience 2022Quote: ... media was collected and concentrated 1:10 in 1xPBS using Lenti-X concentrator (Takara Bio, #631231), aliquoted ...
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bioRxiv - Synthetic Biology 2019Quote: ... Yeast Synthetic Defined (YSD) media was prepared according to the manufacturer recommendations (Takara Bio USA, Inc.). Commercially available woodchip substrate was purchased from Out Grow ...
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bioRxiv - Genetics 2023Quote: ... One-third of the total media volume of Lenti-X Concentrator (631232, Takara Bio, Shiga, Japan) was added to the viral media and refrigerated for 30 minutes at 4°C before being centrifuged for 45 minutes at 1500g and 4°C ...
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bioRxiv - Immunology 2019Quote: Non-tissue culture treated 12-well plates were coated overnight at 4C with 1mL Retronectin (Takara) at 25ug/mL in PBS ...
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bioRxiv - Genetics 2021Quote: ... prepare non-tissue culture treated plates by adding RetroNectin (1 μg/μL; Takara Bio, Otsu, Japan) to enhance transduction efficiency ...
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bioRxiv - Genetics 2022Quote: ... then washed in water and re-suspended in 125-250mL -leu - his galactose liquid culture (Takara Bio Minimal SD Bases ...
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bioRxiv - Microbiology 2020Quote: ... media (0.67% yeast nitrogen base, 0.5% glucose, 0.01% adenine hemisulfate salt) supplemented with -Leu DO supplement (Clontech) (SD-Leu ...
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bioRxiv - Immunology 2021Quote: ... 24-well non-tissue culture plate wells were pre-coated with RetroNectin® (Takara Bio USA, Inc.) according to manufacturer’s instructions and then day 10 cultured Tregs were added at 0.3 x 106 cells/well in 0.3mL of cell culture media ...
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bioRxiv - Neuroscience 2020Quote: ... IPSCs were passaged 15 times before being transferred to the Cellartis DEF-CS 500 culture system (Takara).
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bioRxiv - Microbiology 2020Quote: ... the His-tagged spike protein produced in the culture supernatants was purified with a Talon resin (Clontech).
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bioRxiv - Immunology 2023Quote: ... Transduction was performed on non-tissue culture 24-well plates previously coated with 0.5mL of RetroNectin (Takara) at a concentration of 25 μg/mL in PBS ...
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bioRxiv - Microbiology 2023Quote: ... The culture was then split in half and one tube received 40ul 2ug/ul anhydrotetracycline (Takara, 631310) to induce GFP expression ...