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Citations for Takara Bio :
1 - 50 of 117 citations for Sodium 40 wt. % dispersion in oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: mCherry-2Xflag-mAlix wt (mCherry-Alix) was obtained by subcloning 2xflag-mAlix wt cDNA into a pmCherry-C1 vector (Clontech). Alix-YFP was obtained by subcloning wild type Alix cDNA into a pEYFP-N1 vector (Clontech) ...
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bioRxiv - Neuroscience 2020Quote: ... HE or WT mice using the Fast Pure RNA kit (Takara). The purified RNA samples were used as templates for reverse transcription reactions ...
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bioRxiv - Microbiology 2021Quote: 3xFLAG-D10 WT was cloned through an InFusion cloning reaction (Takara Bio) into the NotI site of pCDNA4 to generate pCDNA4-3xFLAG-D10 WT ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA for p53 WT was cloned into pRetroX-IRES-ZsGreen1 (Clontech). The cDNA for p53 WT or p53 QM was cloned together with the DNA sequence for an NH2-terminal FLAG tag into pRetroX-Tight-Pur (Clontech) ...
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bioRxiv - Genomics 2021Quote: ... 0.5U RNase Inhibitor (Takara; 40 U/μL). After dispensing ...
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bioRxiv - Biophysics 2024Quote: ... wild-type (WT) MDCK cells were transfected with pAcGFP1-Mem vector (Takara, 632509). To decouple the LINC complex by overexpressing dominant-negative KASH protein ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM ATP (Takara, sodium salt, pH 7.0), and 0.5 mM NADPH (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM ATP (Takara, sodium salt, pH 7.0). 500nM enzyme was added to start reaction ...
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bioRxiv - Biochemistry 2020Quote: ... After high speed centrifugation (40 000 x g/40 min/4 °C) the supernatant was loaded on a HiTrap (GE) TALON Crude (Takara) metal affinity column ...
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bioRxiv - Neuroscience 2022Quote: ... 0.25 μl RNAse inhibitor (40 U/μl, Clontech), 2 μl Superscript II First-Strand Buffer (5x ...
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bioRxiv - Genomics 2021Quote: ... 0.04 μL RNAse Inhibitor (Takara; 40 U/μL), 0.5μM oligo-dT (IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... in 40 μl of N2B27 medium (Takara Bio). The plate was centrifuged at 750 rpm for 2 minutes to promote deposition and aggregation of the cells and was then incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... Full length of WT mHVEM and mutants were cloned into pmCherry-N1 vector (Clontech), respectively ...
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bioRxiv - Biochemistry 2021Quote: Flag-GFP-tagged UCH37 (WT, C88A, C88S or EWI) were cloned into pQCXIP (Clontech). Retroviruses were produced by co-transfection of pCI-VSVG (a gift from Garry Nolan ...
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bioRxiv - Biochemistry 2021Quote: ... wt and mutants was induced by treatment with 2 μg/ml Doxycycline (631311, Clontech) for 24-48 hours ...
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bioRxiv - Neuroscience 2021Quote: ... The WT hPNPO cDNA was amplified from the human brain cDNA library (TaKaRa, Cat #637242) [24] ...
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bioRxiv - Cancer Biology 2021Quote: ... The wilde-type (WT) hTERT-immortalized retinal pigment epithelium (hTERT-RPE1, Clontech, Palo Alto, CA) and RPE1–MYCN-ER were cultured in DMEM/F-12 HEPES ...
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bioRxiv - Biophysics 2023Quote: WT and R203M N175-245were purified using the TALON His-tag purification protocol (Clontech Laboratories). Cell pellets were lysed in high salt buffer (50 mM tris ...
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bioRxiv - Molecular Biology 2024Quote: ... pLenti V HELLS WT or Mutant plasmids were constructed using In-Fusion cloning (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... human CALM1 cDNA within the pIRES2-EGFP vector was utilized for WT CaM (Clontech Laboratories). From this WT CaM-pIRES-EGFP construct ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated 40-fold with Lenti-X Concentrator (Takara). The concentrated lentivirus was used immediately or stored at −80°C ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... 0.25 μL 40 U/μL RNAse inhibitor (Takara #2313A), 0.25 μL SSRT IV ...
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bioRxiv - Cell Biology 2020Quote: ... WT ZAP-70 or C564R ZAP-70 was cloned into the pmCherry-C1 vector from Clontech (now Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... the cDNA encoding human Wt-syn was introduced into a vector from Clontech (Mountain View, CA) containing the mRFP sequence ...
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bioRxiv - Genomics 2021Quote: ... 0.48 μL Recombinant RNAse Inhibitor (40 U/μL, Takara Bio), Superscript IV Reverse Transcriptase (200 U/μL ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RNase Inhibitor (Takara Bio/Clontech, #2313A, 40 U/ul), and SMARTScribe RT (Takara Bio/Clontech ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RNase Inhibitor (Takara Bio/Clontech, #2313A, 40 U/ul), and SMARTScribe RT (Takara Bio/Clontech ...
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bioRxiv - Genomics 2021Quote: ... 0.15 μL Recombinant RNAse Inhibitor (40 U/μL, Takara Bio), 0.06 μL Dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 μl Recombinant ribonuclease inhibitor (40 U/μl, #2313, Takara), and 0.1 μl ERCC RNA Spike-In Mix (107 dilution ...
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bioRxiv - Immunology 2021Quote: ... 0.1 µl of 40 U/µl RNase Inhibitor (Clontech Takara) and 2.8 µl of 1 M Trehalose (Life Sciences Advanced Technologies) ...
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bioRxiv - Immunology 2021Quote: ... 0.1 µl of 40 U/µl RNase Inhibitor (Clontech Takara) and 2.8 µl of 1 M Trehalose (Life Sciences Advanced Technologies) ...
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bioRxiv - Genomics 2024Quote: ... 75 µl Recombinant RNase Inhibitor (40 U/ml; Takara, 2313B)) without resuspension ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was amplified from WT genomic DNA by PCR using Advantage 2 Polymerase (#639202, Takara Bio, USA). Plasmid backbones were double-digested with the necessary restriction enzymes and purified using the Qiagen DNA purification kit (#28704X4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Undifferentiated hESC HUES4 WT and PDXeG clone 170-324 were adapted and maintained in DEF-CS (Takara). When reaching approximately 80% confluence ...
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bioRxiv - Physiology 2023Quote: ... with 1.6 µg WT or mutant pcDNA5/FRT/TO Opti-hERG and 0.4 µg peGFP (Clontech, France) for fluorescence-based cell selection in microscopy24 ...
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bioRxiv - Biochemistry 2024Quote: N-terminally HA-tagged IPMK (WT or the kinase deficient D144A mutant63) were cloned into pLPCX (Clontech). VSVg pseudotyped MLV was prepared by transfecting pLPCX-HA-IPMK along with pMLV-Gag-Pol and pVSVg into HEK293T cells using PolyJet transfection reagent (SignaGen) ...
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bioRxiv - Cancer Biology 2024Quote: ... DAXX mutant expression vectors were derived from the WT DAXX expression vector using In-Fusion mutagenesis (Takara) with primers as listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 0.01 µl of RNase inhibitor (40 U/µl; Takara). Immediately after sorting ...
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bioRxiv - Immunology 2020Quote: ... plates were coated with 40 μg/mL retronectin (TAKARA, # T100A/B) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara). The proteins were purified with the glutathione-Sepharose 4B protein chromatography purification kit (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... combined in tandem and integrated into the AscI site in pZH_OsU6sgRNA_SpCas-wt harboring SOG1-7D GT template by an In-Fusion reaction (Takara).
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bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the PacI site in pZH_OsU6sgRNA_SpCas-wt (Endo et al., 2019) using an In-Fusion HD cloning kit (Takara). Two pairs of 20-nt annealed oligonucleotides targeting exon 4 of the OsSOG1 gene were cloned into the BbsI site of the pU6gRNA-oligo (pU6::sgOsSOG1-2 and pU6::sgOsSOG1-3 ...
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bioRxiv - Plant Biology 2021Quote: ... HyPRP expression (in WT/ACD6-1HA) was analyzed by quantitative RT-PCR using SYBR Premix Ex Taq (Takara) in a Bio-Rad Real-Time CFX96TM C1000 system (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... pENTR1A SLX4 WT was used as a template and PCR was performed with the Advantage HD polymerase (Clontech). The PCR product was then digested by DpnI and subsequently phosphorylated and ligated using T4 PNK and T4 ligase (NEB ...
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bioRxiv - Immunology 2023Quote: Wt or mutated human (h)ALPK1 cDNA constructs were cloned into the pCMV-myc plasmid (Takara Bio Inc) and were all siRNA resistant against the hALPK1 siRNA (s37074 ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 5 μL of RNAse inhibitor (Takara 2313B; 40 U/uL). The contents of the dounce were moved to a pre-chilled 15 ml conical tube ...
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bioRxiv - Genomics 2020Quote: The amplified library was then recombined with pCMV FAS wt minigene exon 5-6-7 (Förch et al., 2000) using the In-Fusion HD Cloning kit (639649, Clontech) in a 1:8 vector:insert optimized ratio and transformed into Stellar competent cells (636766 ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Cell Biology 2023Quote: ... 13.32 (WT female) and 13.33 (WT male) were transduced with virus bearing either the empty pLVX-EF1a-IRES-ZsGreen1 vector (Takara), or the same plasmid expressing the coding sequence of C181 as described in lentivirus transduction ...