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Citations for Takara Bio :
1 - 50 of 119 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... small intestine (cat. no. 636539, Takara Bio USA, Inc), and mammary gland (cat ...
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bioRxiv - Genetics 2021Quote: ... Illumina small RNA-Seq kits (Clontech, Mountain View, CA, USA) were then used to generate cDNA libraries ...
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bioRxiv - Physiology 2024Quote: ... Three volumes of concentrated virus medium were subsequently mixed with one volume of Lenti-X concentrator (Takara Bio) and rotated at 4°C overnight before centrifugation (1500 g for 45 min at 4°C) ...
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bioRxiv - Bioengineering 2021Quote: ... 1/3 volume Retro-X concentrator (Takara) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagged proteins were bound to 6 (=3 mL bed volume) or 3 mL (=1.5 mL bed volume) pre-equilibrated Talon SuperFlow Metal Affinity Resin from TaKaRa (LarA wt ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 volume of Lenti-X Concentrator (TAKARA, 631231) was added to 3 volumes of supernatant and incubated at 4°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: ... 1x volume of Lenti-X concentrator (Takara Bio) was combined with 3x volumes of clarified viral media and stored at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 1 volume of Lenti-X concentrator (Clontech, 631231) was added to 3 volumes of clarified-lentivirus containing medium ...
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bioRxiv - Molecular Biology 2021Quote: Small RNA libraries were prepared using the SMARTer smRNA-seq kit (Takara, #635030) with 700ng total RNA ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 1/3 volume Lenti-X Concentrator (Takara 631232), mixed by gentle inversion ...
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bioRxiv - Biochemistry 2020Quote: ... Small RNA libraries were prepared using the SMARTer smRNA-Seq Kit for Illumina sequencing (Takara). Size-selection was performed using Blue Pippin 2% agarose gel cassettes (Sage Science) ...
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bioRxiv - Neuroscience 2023Quote: Small scale AAV cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Microbiology 2023Quote: ... and small intestinal organoids by Total RNA extraction reagent RNAiso Plus (TaKaRa Bio, Shiga, Japan) or NucleoSpin®RNA (MACHEREY-NAGEL ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA libraries were generated using the Small RNA-Seq Kit for Illumina (Takara, Shiga, Japan). cDNA fragments are sequenced by the read length using sequence by synthesis method on the Illumina platform ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNA libraries were prepared using the SMARTer smRNA-seq Kit for Illumina (Takara, 635031) with 12 amplification cycles followed by bead size selection (ratio 0.95x-0.7x) ...
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bioRxiv - Genetics 2022Quote: ... Small DNA fragments were removed by spin-column chromatography with Chroma-Spin-1000+TE columns (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction volume of 25 μl containing 1XPrimeStar Max DNA Polymerase (Takara), 300 nM of forward and reverse primers and 100 ng of DNA ...
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bioRxiv - Bioengineering 2022Quote: ... Capturem large volume filters (Takara Bio USA Inc., San Jose, CA, USA) were used to estimate the quantity of membrane required ...
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bioRxiv - Biophysics 2023Quote: Cells were lysed in the manufacturer’s recommended volume of Trizol (Takara, Japan). RNA was isolated by the chloroform-isopropanol extraction method ...
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bioRxiv - Immunology 2021Quote: ... A2058 and MDST8 as previously described (7) with small changes for adherent cell lines omitting RetroNectin (Clontech). LCL-1 was transduced with single minigenes ...
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bioRxiv - Microbiology 2020Quote: ... master mix was used to yield a final volume of 20 µL (Takara). The thermal cycling conditions were 5 min at 95 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Twenty μL reaction volumes containing 10 μL SYBR premix ExTaq (TAKARA, Dalian, China), 0.6 μL of each of the forward and reverse primers ...
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bioRxiv - Bioengineering 2024Quote: ... mixed with one-tenth of the volume of Lenti-X Concentrator (Takara Bio), and incubated at 4 °C for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribo-seq libraries were then prepared with the purified footprints and the SMARTer Small RNA-Seq Kit (Takara, 635029) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... the media was incubated with ⅓ volume of Lenti-X Concentrator (Takara, Cat No. 631231) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... 10 μL reaction volume of TB Green® Premix Ex TaqTM II (TaKara, Japan), which included 40ng fecal DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was purified from a small section of embryos using SimplePrep™ reagent for DNA (TaKaRa Bio, Kusatsu, Japan).
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bioRxiv - Plant Biology 2021Quote: ... Confirmation of interaction was performed by targeted transformation of the specific constructs using the small-scale yeast transformation protocol as described in the yeast protocol handbook (Clontech). Transformants were grown in YPDA media to OD600 de 0,5 – 0,8 ...
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bioRxiv - Immunology 2022Quote: ... RNA concentrations were determined using the Qubit high-sensitivity RNA assay and small RNA libraries were created using the Clontech SMARTer smRNA-Seq preparation kit according to manufacturer instructions (Takara). Small RNA inserts were further enriched using AMPure bead selection (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was then used for library preparation using the SMARTer small RNA (smRNA)-seq kit for Illumina (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Primers designed to flank the MLR of human CDHR5 were used for PCR reactions with Human Small Intestine QUICK-Clone cDNA (Clontech) as the template ...
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bioRxiv - Biophysics 2020Quote: ... and allowed to bind to cobalt resin (Talon, Clontech; 1 mL bed volume/L culture). The column was washed sequentially with cobalt wash buffer (CoWB ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of cell lysate was added to equal volume of LDH substrate (Takara, France) in a 96-well microplate and incubated at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the final volume was increased to 1 mL using SOC medium (cat. no. ST0215, Takara) pre-warmed to 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared from <=2.5ng input RNA using 1/4 volume SmartSeqv4 technology (Takara Bio) and sequenced on NextSeq High Output flow cells ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatant was recovered and mixed with a third volume of LentiX Concentrator (Takara Bio), incubated at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... spectrophotometer followed by reverse transcription reaction to produce cDNA using PrimeScript RT Reagent Kit (Takara, Clonetech, Japan). Specific primers against the genes of interest (Table 3 ...
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bioRxiv - Plant Biology 2021Quote: Yeast strain AH109 was transformed with bait (pGBKT7) and prey (pGADT7) constructs by following the small scale yeast transformation protocol from Yeastmaker™ Yeast Transformation System 2 (Clontech). Upon transformation ...
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bioRxiv - Molecular Biology 2022Quote: Synthesis of cDNA with biotinylated 3’ adaptor ligation was performed using a Small RNA Cloning Kit (Takara Bio Inc., Kusatsu, Japan) as previously reported (20 ...
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bioRxiv - Genomics 2020Quote: ... 250 nL volume ICELL8 chip (SMARTer™ ICELL8® 250v Chip, Takara Bio USA, CA, USA) using a Multi Sample Nano Dispenser (MSND ...
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bioRxiv - Genomics 2021Quote: ... The dCas9KRAB lentivirus was concentrated 20X the initial media volume using Lenti-X concentrator (Clontech, 631232), following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... The 20 µL PCR reaction volume included 10 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Genetics 2022Quote: ... in a 10 µL reaction volume with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) following the recommended protocol ...
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bioRxiv - Genetics 2023Quote: ... One-third of the total media volume of Lenti-X Concentrator (631232, Takara Bio, Shiga, Japan) was added to the viral media and refrigerated for 30 minutes at 4°C before being centrifuged for 45 minutes at 1500g and 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Amplifications were typically performed in 20 μL reaction volumes containing 1× EmeraldAmp GT PCR Master Mix (Takara Bio Inc. ...
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bioRxiv - Genetics 2022Quote: ... in a 25 µl reaction volume with buffer and dNTPs provided with the PrimeSTAR GXL polymerase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set, Clontech Laboratories, Cat# 635651). The filtered supernatant was loaded onto the HisTALON cartridge (Clontech Laboratories ...
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bioRxiv - Genomics 2019Quote: ... in a 20-μL reaction volume with 10 μL of 2× SYBR Premix Ex Taq II (TaKaRa), 2 μL of cDNA ...
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bioRxiv - Plant Biology 2020Quote: ... The 20-µL reaction volume comprised 10 µL 2× SYBR Green PCR Master Mix (Takara, Dalian, China), 0.2 µM each primer ...