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Citations for Takara Bio :
1 - 50 of 649 citations for Sheep Anti CMV Pentamer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... in which the CMV promoter of pAAV-CMV vector (Takara Bio) was replaced by gfaABC1D ...
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bioRxiv - Microbiology 2020Quote: ... CSII-CMV-MCS-IRES2-Bsd (RIKEN BRC) and pLVSIN-CMV Pur (Takara Bio), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... The CMV-MitoDsRed2 (pDsRed2-Mito) and CMV-MitoGFP (pAcGFP1-Mito) plasmids are from Clontech. Atoh7-MitoDsRed2 was designed using UGene software (Unipro) ...
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bioRxiv - Immunology 2020Quote: ... 1.3 μg CMV 8.91 (Clontech), and 1.5 μg of target vector were transfected using TransIT-293T transfection reagent (Mirus Bio) ...
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bioRxiv - Microbiology 2020Quote: ... The CMV-GFP construct was pEGFPC1 (Clontech). HIV-1 bearing a Ba-L envelope gene has been described (Rasaiyaah et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... These coding gene sequences were all cloned into CMV-based FLAG-tagging and Clover-tagging vectors (CMV-FLAG-J1 and CMV-Clover-J1; derived from commercially available vectors pEGFP-C1 and pEGFP-N1; Clontech) for ectopic expression in human cells.
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bioRxiv - Neuroscience 2022Quote: ... Mover-IRES-palmGFP (promoter: CMV; backbone: Clontech N1); mGFP-Mover (promoter ...
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bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
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bioRxiv - Cell Biology 2022Quote: ... the CMV promoter from pEGFP-C1 (Takara Bio Inc) was removed and replaced with the SEPTIN2 promoter using AseI and AgeI ...
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bioRxiv - Cancer Biology 2024Quote: ... pHTN HaloTag® CMV-neo and pHTC HaloTag® CMV-neo were digested with restriction enzymes and subsequently ligated (Takara, cat n°6023). Primers and corresponding restriction digests can be found in Table S4 ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFP and mCherry vectors were from Clontech (with CMV promoter) with either C1 or N1 cloning sites to obtain the tag either at the N-terminus or C-terminus of Arc ...
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bioRxiv - Neuroscience 2023Quote: ... Cousin lab was dropped into an pEGFP-N1 CMV mammalian expression vector (Clontech) by In-fusion cloning ...
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bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
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bioRxiv - Microbiology 2019Quote: ... was first produced by modifying a CMV-driven eGFP cassette from pEGFP-N1 (Clontech), such that the CMV immediate early promoter was partially duplicated with an iscei::kan cassette inserted between the duplicated sequences ...
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bioRxiv - Microbiology 2023Quote: ... and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB ...
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bioRxiv - Biophysics 2021Quote: ... Protein constructs were expressed under control of a CMV promoter using pEGFP-C1/N1 (Clontech) (enhanced GFP ...
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bioRxiv - Immunology 2022Quote: ... and then the expression construction of CMV-tdTomato was amplified from pCMV-tdTomato vector (Clontech, 632534) to inserted downstream of HA-R by GeneArt Gibson Assembly (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... coding regions were cloned downstream of the CMV promoter in derivatives of the pEGFP-N1 plasmid (Clontech) with an NLS and linker sequences to EGFP or mCherry (Supplemental Table 2) ...
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bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... a plasmid expressing mCherry under the CMV promoter (pmCherry) was cloned by removing EGFP from pEGFP-C1 (Clontech) with AgeI and BglII and assembling the vector with a synthesized mCherry gene fragment (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... We used IF cloning for the pBI dual expression vector containing a minimal CMV promoter (Takara Bio USA) (Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... which was cleaved with Xba1 and Cla1 and ~2.6 kbp CMV-IRES-puror Xba1+Cla1 fragment from pQCXIP (Clontech) was inserted to construct pHIV-CMV-IRES-puro ...
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bioRxiv - Molecular Biology 2019Quote: ... The fragment was excised from the pMD18-T vector and ligated into the pLVSIN CMV Puro destination vector (TaKaRa) resulting in the pLVSIN-miR-378/486 vector ...
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bioRxiv - Immunology 2021Quote: The cDNA for different FcRs was cloned in fusion with EGFP in the pLVX-CMV-IRES-Puro plasmid (Clontech). The cDNA coding for FcγRI was amplified from the NM_000566 cDNA (Origene ...
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bioRxiv - Cancer Biology 2021Quote: ... with PCR using the primers listed in Table 1 and cloned into pmCherry-C1 (CMV promoter; Takara Bio Inc.). pEGFP-C3 K19 WT ...
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bioRxiv - Cell Biology 2023Quote: AAV transfer plasmids were constructed by removing all the sequences between the ITRs of the pAAV-CMV vector (TaKaRa) and inserting the Mhck7 promoter ...
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bioRxiv - Developmental Biology 2020Quote: The ERK-KTR-mCherry construct for transient expression was prepared by first inserting the coding sequence for ERK-KTR (Regot et al., 2014) into a CMV-driven mCitrine C1 expression vector (TaKaRa), and then replacing the fluorophore for mCherry ...
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bioRxiv - Cancer Biology 2019Quote: ... All the amplicons were cloned into a lentiviral plasmid pCSII–CMV–MCS (RIKEN, RDB04377) by using the In-Fusion HD Cloning Kit (TaKaRa), to produce the pCSII– CMV:A3B–3×FLAG–IRES–EGFP donor DNA plasmid.
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bioRxiv - Biochemistry 2021Quote: ... DNA was gel purified and inserted into pCDNA 3.1 vectors (CMV promoter) by making use of In-Fusion HD Cloning Kits (Takara Bio) as described in previous section ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was gel purified and inserted into pCDNA 3.1 vectors (CMV promoter) by making use of In-Fusion HD Cloning Kits (Takara Bio). EcoR1 and HindIII digested vector was incubated with overlapping PCR fragments (of various different recombinant DNAs ...
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bioRxiv - Developmental Biology 2020Quote: ... It was subsequently cloned into BamHI site in the pLenti CMV-IRES-GFP by In-Fusion cloning kit (Clontech, 639649) to generate lentiviral packaging plasmids(pLentiCMV-Y437H-IRES-GFP) ...
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bioRxiv - Biochemistry 2022Quote: ... at the NotI/BamHI sites or into 8xHis-MBP pFastBac1 modified with a CMV promoter (obtained from David Julius’ lab) at the BamHI/NotI sites using In-Fusion EcoDry cloning (Takara) according to manufacturer protocols ...
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bioRxiv - Microbiology 2022Quote: ... gRNAs targeting the selected region of the CMV and bGH sequences (235-310 and 1917-2029, respectively in pEGFP-N3, Takara), were designed accordantly to each ROI (Table S3 and Supplementary Methods) ...
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bioRxiv - Cancer Biology 2019Quote: ... The cDNA was then ligated into pCDH-CMV-MCS-EF1-GFP using Takara ligation Kit (Clontech, Mountain View, California, USA) and was confirmed in the expression vector by DNA sequencing at The Centre for Applied Genomics (SickKids ...
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bioRxiv - Microbiology 2020Quote: ... For analysis of the NAD metabolome HEK293T cells were transfected with 1µg of pEGFP-C1 Empty Vector or pEGFP-C1-CMV-PARP10 using CalPhos Mammalian Transfection Kit (Takara Bio). 6 hours later the cells were treated with chemical treatments in DMEM + 10% FBS at 37°C 5% CO2 for 18h.
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNAs were subcloned into the pCK-FLAG vector (CMV promoter-driven vector) at the BamHI and XhoI sites using In-Fusion HD Cloning Kit (Clontech). For dsRBD-deleted DICER ...
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bioRxiv - Cell Biology 2021Quote: ... Expression from the CMV-TetO2 promoter in Flp-In T-REx cells was induced by treatment with 10 ng/mL doxycycline (Clontech) for 24 hours ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... ORFV120 and ORFV113 coding sequences were PCR-amplified from orf virus strain OV-IA82 genome and cloned into p3xFlag-CMV-10 vector (pFlag) (Clontech).
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bioRxiv - Immunology 2021Quote: Variable heavy chain and light chain sequences were PCR amplified from yeast plasmid DNA and cloned into CMV/R IgG expression vectors using InFusion (Takara). Plasmid DNA for mammalian expression of the His-tagged SARS-CoV-2 RBD in a pCAGGS vector was kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: ... The PGK promoter-puromycin resistance gene cassette in the vector was further replaced by the CMV-eGFP cassette from the pEGFP-C1 vector (Clontech). The sequence-verified plasmid was transfected into Lenti-X 293T cells (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... The biosensor expression cassette was transduced into the stable tet-OFF MCF10A cell line under the regulation of a tet-inducible minimal CMV promoter (pRetro-X-Puro; Clontech) and selected using puromycin for a stably integrated population ...
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bioRxiv - Cell Biology 2022Quote: ... All above sequences along with MBNL1 and MBNL2 isoforms imaged in primary neurons were cloned downstream of GFP in a pEGFP-C1 plasmid backbone under control of a CMV promoter (Takara). Full-length kinesin sequences were amplified from mouse cDNA and inserted upstream of an mScarlet-containing plasmid in a pcDNA3.1 backbone under the control of CMV promoter.
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bioRxiv - Immunology 2022Quote: ... PGDM1400 heavy and light chain sequences were synthesized (Integrated DNA Technologies) and cloned into a mammalian expression vector under a CMV promoter using InFusion (Takara) and sequence verified.
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bioRxiv - Neuroscience 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA at the BstXI and XhoI restriction enzyme sites using the In-Fusion HD cloning kit (Clontech). rAAV vectors were generated using similar plasmids and cloning methods as was referenced in (Matharu et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Mammalian expression vector for Flag-EBNA1 contained B95-8 EBNA1 lacking the GA repeats (aa 101-324) under the control of CMV-3XFLAG promoter in a plasmid derived from pREP10 (Clontech) containing ...
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bioRxiv - Synthetic Biology 2023Quote: ... each for a 50-µL reaction containing ∼30 fmol of EcoRI-XbaI digested pLVSIN-CMV-Pur backbone plasmid (Takara #6183), ∼300 fmol of the insert fragment ...
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bioRxiv - Biochemistry 2024Quote: ... an attB recombination site was inserted in place of the CMV promoter by InFusion HD Cloning (Takara Bio, Shiga, Japan). To facilitate the creation of these libraries using nicking mutagenesis ...
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bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
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bioRxiv - Immunology 2024Quote: An adenovirus plasmid encoding the first 30 amino acids of KRAS with the G12V mutation (KRAS G12V-30) was created following the manufacturer’s instructions (Adeno-X CMV, Takara Bio). Adenovirus particles were produced by transfecting the adenoviral plasmid into HEK293 cells ...
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bioRxiv - Neuroscience 2021Quote: ... These were then packaged into lentivirus via cotransfection with CMV-Gag-Pol (Harvard #dR8.91) and pVSV-G (Clontech, part of #631530) plasmids into production HEK293T cells adapted to grow in serum-free conditions (OHN media ...