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Citations for Takara Bio :
151 - 200 of 4823 citations for Serum Zinc Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Y2H assays were carried out using the Matchmaker two-hybrid system (K1605, Clontech). One of the baits pGBT9 ...
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bioRxiv - Bioengineering 2024Quote: ... VLP were quantified by a LFA assay for HIV p24 (GoStix Plus, Takara). In this case the number of virions per volume is calculated by the approximate stoichiometry of p24 in VLP (∼2000/virion) ...
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bioRxiv - Plant Biology 2020Quote: Yeast two hybrid assay was performed using Matchmaker Gold Yeast Two-Hybrid System (Clontech). The OsSalT gene was cloned in-frame into the EcoRI and BamHI RE sites of the yeast vector ...
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bioRxiv - Plant Biology 2021Quote: ChIP-Seq assays were performed on whole seedlings using anti-GFP antibody (Clontech 632592). Seedlings at 14 DAS (5 g ...
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bioRxiv - Plant Biology 2021Quote: ... Liquid β-galactosidase assays were performed as described in the Yeast Protocols Handbook (Clontech). A Y2HGold yeast strain containing a bait vector was mated with the Y187 strain containing the pGADT7 vector and selected on SD/-Trp/-Leu media ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR assay was performed using the TB Green Fast qPCR Mix (Takara). The ribosomal protein L21 gene (RPL21 ...
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bioRxiv - Cell Biology 2019Quote: Total protein was extracted from cells by lysing with radioimmunoprecipitation assay buffer (Takara, Japan). Ten micrograms of protein were run on 4–20% gradient polyacrylamide gels (Thermo Fisher ...
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bioRxiv - Plant Biology 2022Quote: Yeast one-hybrid assays were performed using the Matchmaker Yeast One-Hybrid System (Clontech). The DNA fragment 1,000 bp upstream of the start codon ATG of DDRM2 was cloned into pHis2.1 vector and SOG1 was cloned into the pGADT7 vector ...
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bioRxiv - Microbiology 2023Quote: RNA obtained from the RIP assay sample was purified using NucleoSpin RNA Plus (TaKaRa), followed by library construction and RNA-seq analysis ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed with the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio). Saccharomyces cerevisiae strain AH109 was co-transformed with different pairs of pGADT7 and pGBKT7 harboring IREH1 or B1-Rafs ...
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bioRxiv - Plant Biology 2023Quote: ... the Y1H assays were performed following the manufacturer’s instruction (Clontech Laboratories, Inc., Kusatsu, Japan). The promoter sequence with and without mutation in ZDRE motif were artificially synthesized and cloned into the Kpn I-linearized pAbAi vector (Clontech Laboratories ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Molecular Biology 2021Quote: ... DMEM supplemented with 10% fetal bovine serum (FBS) and 10 μl DNase I (Takara Bio, Shiga, Japan) was then added to the samples and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... 3% normal goat serum (NGS) and then incubated overnight with 1:2000 rabbit-anti-DsRed (632496, Clontech) (Geerling et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The concentration of the purified protein was assessed by densitometry using bovine serum albumin (TaKaRa, cat. # T9310A) as a standard following SDS-PAGE and staining with the Q-stain ...
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bioRxiv - Plant Biology 2020Quote: Y1H assay was performed using Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The nucleotide sequences of promoter region of selected key defense genes having potential RAV1 binding motifs were retrieved by using online tool (https://bioinformatics.psb.ugent.be/plaza/) ...
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bioRxiv - Plant Biology 2021Quote: ... Yeast two hybrid assays were performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The standard protocol given in the kit was followed for Y2H assay ...
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bioRxiv - Plant Biology 2021Quote: ... β‐galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two‐Hybrid System; Takara Bio ...
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bioRxiv - Microbiology 2020Quote: The yeast two-hybrid assays were performed in strain Saccharomyces cerevisiae Y2HGold (Takara Bio USA). Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research) ...
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bioRxiv - Genetics 2019Quote: The yeast two-hybrid assays were performed using the Matchmaker Yeast Two-Hybrid System (Clontech). Various fragments from OsLUGLWT and OsLUGLkkx were cloned into pGBKT7 ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed by the Matchmarker GAL4-based two-hybrid System (Clontech). Constructs were co-transformed into the yeast strain AH109 by the lithium acetate method (Gietz & Woods ...
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bioRxiv - Microbiology 2019Quote: The dual-luciferase assay using an NF-□B–dependent firefly luciferase (pNF-□B-Luc; Clontech) and a Renilla luciferase driven by the thymidine kinase promoter (pRLTK ...
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bioRxiv - Genetics 2022Quote: ... and then yeast two-hybrid assays were performed in a Gal4-based system from Clontech in a diploid strain by mating as previously described (Rossignol et al ...
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bioRxiv - Plant Biology 2022Quote: Nuclear two-hybrid interaction assays were performed using the MATCHMAKER GAL4 two-hybrid system (Clontech). The CWLP coding region without signal peptide (SP ...
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bioRxiv - Plant Biology 2022Quote: ... The Y2H assays were carried out in the Matchmaker Gold Yeast Two-Hybrid System (Clontech). The pairs of corresponding recombinant constructs fused with pGBKT7 BD and pGADT7 AD were co-transformed into the Y2H Gold yeast competent cells ...
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bioRxiv - Plant Biology 2023Quote: ... Yeast one-hybrid (Y1H) assays were performed according to the manufacturer’s instruction (Clontech, CA, USA). The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA isolation and real-time PCR assay Total RNA was extacted by RNAiso Plus (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pre-treated 1 hour prior to the assay with A/C Heterodimerizer (Takara) diluted at 1:200 in complete media concurrent with JF-Halo or -SNAP dye labeling ...
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bioRxiv - Microbiology 2023Quote: Y2H assays were performed according to Yeastmaker™ Yeast Transformation System 2 User Manual (Clontech). The coding sequences corresponding to HCPro1 ...
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bioRxiv - Cell Biology 2024Quote: Following plasmids were used in this assay to generate stable cell lines: pCMV-HRasG12V (Clontech), Piggyback vectors ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A) in Bio-Rad iQ5 system ...
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bioRxiv - Immunology 2022Quote: ... Seq Kit (Takara), as per the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio), GAPDH was used as internal reference ...
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bioRxiv - Neuroscience 2023Quote: All cells were cultured in high glucose DMEM with 10% Fetal Bovine Serum (tetracycline free, Clontech or Gibco) and penicillin/streptomycin 50units/ml-50 mg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... In-fusion HD kit and In-Fusion Snap Assembly kit (TaKaRa). pRSETa_mEos4b was a gift from Loren Looger (Addgene plasmid #51073 ...
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bioRxiv - Cancer Biology 2023Quote: ... LipofectamineTM 2000 kit and reverse transcription kit were purchased from TaKaRa Company ...
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bioRxiv - Plant Biology 2019Quote: Yeast two-hybrid assays were performed using the Matchmaker™ gold Yeast two-Hybrid Systems (Clontech). Different truncated CDS of EcaICE1 without transcriptional activation activity and EcaSIZ1 were subcloned into pGBKT7 and pGADT7 vectors to fuse with the DNA-binding domain (DBD ...
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bioRxiv - Plant Biology 2020Quote: Yeast two-hybrid assays were performed according to the Yeast Protocol Handbook (Clontech, catalog no. P3024). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
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bioRxiv - Cancer Biology 2021Quote: Yeast two-hybrid assay was conducted using Matchmaker GAL4 two-hybrid system 3 (Clontech/Takara, USA). Bait and prey vectors were co-transformed into the yeast strain AH109 (Clontech/Takara ...
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bioRxiv - Molecular Biology 2019Quote: Yeast two-hybrid assays were performed using the Matchmaker GAL4-based two-hybrid system 3 (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Y1H assays were performed using the Matchmaker Gold Yeast One-Hybrid Library Screening System (Clontech, USA). The sequence of LhWRKY44 was inserted into the vector pGADT7 as prey ...
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bioRxiv - Developmental Biology 2023Quote: Yeast two-hybrid assays were performed according to the Yeastmaker Yeast Transformation System 2 manual (Clontech). The yeast strain AH109 was co- transformed with an AD-fused and a BD-fused construct using the lithium acetate method ...
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bioRxiv - Bioengineering 2024Quote: ... SEAP production was analyzed by collecting medium and running a chemiluminescent assay on samples (Takara Bio) using a Tecan Infinite M1000 Pro plate reader.
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bioRxiv - Developmental Biology 2022Quote: ... followed by the addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100 [Nacalai Tesque], 0.7 μL of 2% bovine serum albumin [BSA] [Takara Bio]) and incubation at 70°C for 90 sec to quench the denaturing effect of SDc ...
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bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
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Diversified expression of gamma-protocadherins regulates synaptic specificity in the mouse neocortexbioRxiv - Neuroscience 2021Quote: LATaq Kit (RR002B, TaKaRa) was used in nested PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’RACE kit (Takara) was used to convert RNAs of the prostate tumors into cDNAs by a reverse transcriptase and oligo-dT adapter primer ...
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bioRxiv - Plant Biology 2020Quote: ... β-galactosidase assay was performed according to the manufacturer’s protocol (Matchmaker Two-Hybrid System; Takara, https://www.takarabio.com).