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Citations for Takara Bio :
301 - 350 of 805 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies: Living Colors Polyclonal anti-mCherry/dsRed (rabbit, 1:500, Clontech 632496), anti-GFP (chicken ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry-labeled PACSIN2 was prepared by subcloning PACSIN2 cDNA into pmCherry-C1 vector (Clontech), in which the GFP in pEGFP-C1 was replaced with mCherry ...
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bioRxiv - Neuroscience 2019Quote: ... or IMPA1L 120nt fragment were labeled using Random Priming Labeling Kits (Takara or Roche) and [α−32P] dCTP ...
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bioRxiv - Immunology 2023Quote: ... Probes (50 ng) were labeled with 32P using the Ladderman DNA labeling kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 32P-labeled dsRNAs were synthesized using the in vitro T7 Transcription kit (Takara Bio) with [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Biophysics 2021Quote: ... A cDNA coding for 128QHTT with C-terminal fusion to a FLAG-His affinity tag was cloned into the vector pTRE-tight-BI-AcGFP1 (Clontech) for expression of 128QHTT upon induction with Dox ...
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bioRxiv - Biochemistry 2020Quote: ... After high speed centrifugation (40 000 x g/40 min/4 °C) the supernatant was loaded on to a gravity TALON metal affinity column (Takara), equilibrated and washed with 10 CV buffer A/20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The precipitated material was removed by centrifugation and the soluble fraction was loaded onto a column (5 mL) containing Talon affinity resin (Clontech) equilibrated in buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... The induction of His-and GST-fusion proteins and their subsequent purification using TALON® Metal Affinity Resin (Clontech Laboratories) and Glutathione SepharoseTM 4 Fast Flow beads (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... Non-solubilised material was removed by ultracentrifugation and the soluble fraction was incubated with Talon metal affinity resin (Takara Bio) for 16 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... was then added to the supernatant to a final concentration of 5 mM and the solution was subjected to immobilized metal affinity chromatography (IMAC) by incubation with TALON cobalt resin (Takara) for 1 hr at 4°C ...
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bioRxiv - Biochemistry 2019Quote: Hexa-histidine-tagged scaffolding protein (6 mg) was loaded on a 1 ml immobilized metal affinity chromatography column charged with cobalt (Clontech). Coat protein monomers (0.2 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Purification procedure was similar to that for HEK 293 cells except for the usage of cobalt-based affinity resin (Talon, Clontech) to capture His-tagged proteins.
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bioRxiv - Cell Biology 2021Quote: ... Co-immunoprecipitation and SDS-PAGE followed by western blotting was carried out as mentioned above except TALON metal affinity resin (Takara) was used instead of α-GFP beads for 6His-Skb1 enrichment.
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bioRxiv - Molecular Biology 2022Quote: ... Lysate was cleared by centrifugation at 43 550×g and afterwards bound to 1 mL 50% slurry of Talon Metal Affinity Resin (TaKaRa) equilibrated in lysis buffer without PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were clarified by centrifugation at 3,000 x g and incubated with Talon® immobilized metal affinity resin (Takara #635502) for 2 hours at 4ºC ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged AmiC was isolated from the protein sample using a TALON® metal affinity resin (Takara Bio USA, Inc) as previously described (51 ...
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bioRxiv - Molecular Biology 2022Quote: ... the NaCl concentration of the cleared lysate was adjusted to 500 mM and the cleared lysate was applied to 10 mL (=5 mL bed volume) equilibrated Talon SuperFlow Metal Affinity Resin (TaKaRa) per L culture on ice for 45 min ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 2 mL of washed TALON® Metal Affinity Resin beads (Takara). The mixture was allowed to bind under nutation at 4℃ for 1 hr ...
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bioRxiv - Biophysics 2024Quote: ... The cell lysate was then clarified with centrifugation and the supernatant was collected and loaded onto a gravity column manually loaded with TALON Metal Affinity Resin (Takara), washed 5 times with 10 column volumes (CV ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was collected after centrifugation at 65,000 g for 35 min and was incubated with TALON® Metal Affinity Resin (TaKaRa) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cfr was purified by Talon chromatography (Clontech) from clarified lysates ...
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bioRxiv - Cancer Biology 2019Quote: ... purified with NucleoBond Xtra Midi kit (Clontech), and sequenced (Genewiz).
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using Talon resin (Clontech), eluted in 200 mM imidazole ...
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bioRxiv - Evolutionary Biology 2021Quote: ... purified plasmids with NucleoSpin Plasmid EasyPure (TaKaRa), and sequenced one plasmid for each NSP and MA gene from each of the 218 larval samples ...
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bioRxiv - Neuroscience 2022Quote: ... from purified RNA (NucleoSpin RNA kit; Takara). Raw data from the QuantStudio 5 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... and purified by gel extraction (Clontech Libraries).
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bioRxiv - Genomics 2021Quote: ... and purified on a Chromaspin column (Clontech). All constructs were verified by Sanger sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting band was gel-purified (Clontech) and sequenced by Sanger Sequencing ...
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bioRxiv - Microbiology 2022Quote: ... and purified by Lenti-X Concentrator (TaKaRa). To measure the infectivity ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Fabs were purified by cobalt chromatography (Takara) and further purified over an S200 column on an AKTA pure (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... purified using the AAVpro purification kit (Takara), and the titer was determined using ddPCR ...
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bioRxiv - Bioengineering 2023Quote: ... or purified with Lenti-X Concentrator (Takara). All virus was titrated with Lenti-X GoStix Plus (Takara ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was incubated in primary antibody (DsRed rabbit polyclonal 1:400, Takara, Kusatsu, Japan) overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... guinea pig polyclonal anti-RAX (1:200; M229 Takara Bio Europe Ab, Goteborg, Sweden); rabbit polyclonal anti-activated caspase 3 (AC3 ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3) in ExpressHyb solution (Clontech) and scanned after exposition with Typhoon FLA 9500 scanner (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were centrifuged (1h, 4°C, 14000 xg) and the soluble fraction was passed through 1mL of Talon Metal Affinity Resin (Clontech #635509). The resin was washed for four times with 4mL Wash Buffer (50mM Tris pH7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... The lysate was loaded onto a prepared column with 2 mL TALON Metal Affinity Resin (Clontech Laboratories, Inc., Mountain View, CA). After being washed twice with 5 column volumes (CV ...
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bioRxiv - Biochemistry 2022Quote: ... Polyhistidine tagged IDE was isolated from the soluble lysate fraction by immobilized nickel affinity chromatography (NI-IDA resin, Takara Clontech Inc.) in accordance with manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Polyhistidine tagged IDE was isolated from the soluble lysate fraction by immobilized nickel affinity chromatography (NI-IDA resin, Takara Clontech Inc.) in accordance with manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were centrifuged (1h, 4°C, 14000 xg) and the soluble fraction was passed through 1mL of Talon Metal Affinity Resin (Clontech #635509). The resin was washed for four times with 4mL Wash Buffer (50mM Tris pH7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... the clarified supernatant from the crude extract was carefully collected and mixed with TALON Metal Affinity Resin (Takara Bio, Cat# 635502) for 1 hour to allow for the binding of His-tagged AlkB protein to resin ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were then cleared by centrifugation at 12,000xg for 12 minutes and added to 1ml of pre-equilibrated TALON metal affinity resin (Takara Bio, 635502). The slurry was rotated at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified RNA fragments were treated with CIP (Takara) and ligated with 3’ linker adaptor by using T4 RNA ligase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... The transposed DNA fragments were purified by TaKaRa MiniBEST DNA Fragment Purification Kit (No ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using amylose-agarose resin (Clontech) and eluted in 10 mM maltose ...
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bioRxiv - Microbiology 2021Quote: ... and protein purified using TALON resin (Takara Bio) using standard protocols in 50 mM phosphate buffer pH7.4 containing 300mM NaCl.
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bioRxiv - Molecular Biology 2020Quote: ... The complex was purified using TALON beads (Clontech) after nucleic acid precipitation using 0.05% of PolyEthylImine (PEI ...
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bioRxiv - Immunology 2019Quote: ... Fabs were purified using Cobalt-TALON resin (Takara) followed by gel filtration chromatography on Superdex 200 Increase (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... proteins were purified using Talon resin (Takara Bio) by resuspending the cell pellet in lysis buffer (50 mM phosphate buffer and 300 mM NaCl ...