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Citations for Takara Bio :
151 - 200 of 407 citations for Rubella Virus VLP strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio, 631231) to a titer sufficient to decrease pStat1 levels by 25% (Mean pStat1+ CD11b+ cells ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was produced by using Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (TaKaRa, Japan) with a reverse primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Virus was collected after 48 hours and concentrated with Lenti-X Concentrator (Clontech, Cat#631231) (16) ...
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bioRxiv - Microbiology 2022Quote: ... The purified virus titer was determined using an Adeno-X Rapid Titer kit (Takara Bio). Sex and age at sampling for the cells were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The virus titer was measured using Lenti-X GoStix Plus assay kit (Takara Bio, 631280).
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-X GoStix Plus were then used to determine virus titer (Takara Bio cat#631280). For lentiviral infection ...
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bioRxiv - Immunology 2024Quote: ... The virus particles were then purified using the Aneno-X Maxi Purification Kit (Takara Bio), and the buffer was replaced with 1X Formulation Buffer containing 2.5% glycerol (w/v) ...
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bioRxiv - Microbiology 2024Quote: ... A3 encapsidation was determined by concentrating the virus containing supernatant using Retro-X Concentrator (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Virus was plated on non-tissue culture treated 24-well plates precoated with retronectin (TaKaRa) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Virus titer was measured by using Lenti-X p24 Rapid Titer Kit (Cat #022261, Takara).
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bioRxiv - Plant Biology 2019Quote: ... Pairwise combinations of vectors were co-transformed into the yeast strain Y187 (Clontech) and plated onto the same selective medium ...
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bioRxiv - Cell Biology 2020Quote: ... The Y2H assays were performed in the Y2H Gold strain (PT4084-1, Takara 630489 ...
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bioRxiv - Genetics 2019Quote: ... Pairs of plasmids were transformed into strain Y187 (Clontech, Mountain View, CA, USA) and liquid β-galactosidase assays were performed using an OMEGASTAR plate reader (BMG Labtech ...
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bioRxiv - Plant Biology 2022Quote: ... Bait and prey constructs were co-transformed into the yeast strain Y2HGold (Clontech) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research ...
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bioRxiv - Microbiology 2019Quote: ... These constructs were amplified in the DH5 strain of E.coli (Takara, Dalian, China). Cells were seeded in 6-well plates at the concentration of 5 × 104 cells/well and cultured for 24 h before transfection ...
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bioRxiv - Genetics 2021Quote: ... Saccharomyces cerevisiae strains AH109 Gold and Y187 (Clontech, MATCHMAKER GAL4 Two-Hybrid System) were transformed according to the classical protocol (42 ...
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bioRxiv - Plant Biology 2022Quote: ... pairwise AD and BD were co-transformed into yeast strain Y2H gold (Clontech). Cells transformed with both plasmids were selected after growth 2 d at 30□ on synthetic dropout medium lacking leucine and tryptophan ...
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bioRxiv - Plant Biology 2022Quote: All yeast constructs were transformed into the Saccharomyces cerevisiae Y2H Gold strain (Clontech) using Yeastmaker™ Yeast Transformation System 2 (Clontech) ...
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bioRxiv - Plant Biology 2022Quote: The pGBKT7 and pGADT7 plasmids were co-transformed into yeast strain AH109 (Clontech) using the LiCl-PEG (polyethylene glycol ...
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bioRxiv - Plant Biology 2022Quote: The yeast strain Y2HGold and the Matchmaker Gold Yeast Two-Hybrid System (Clontech) were used for the yeast two-hybrid assay ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was collected at 48 hours and 72 hours and concentrated with Lenti-X Concentrator (Clontech). The Virus was resuspended in DMEM and stored at -80C.
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bioRxiv - Cell Biology 2019Quote: ... after which virus-containing supernatants were harvested and concentrated ∼40-fold using Lenti-X Concentrator (Clontech) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The virus was then quantified using the Lenti-X™ p24 Rapid Titer Kit (Takara Bio) and aliquots were frozen at −80° C for future use.
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... The virus supernatant was harvested 72 hrs post-transfection and concentrated using Lenti-X Concentrator (Clontech). Panc1 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Concentrated virus was plated on non-TC treated 6-well plates coated with retronectin (Takara T100B), and spun for 90 minutes at 1200xg ...
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bioRxiv - Immunology 2020Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Immunology 2020Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Microbiology 2023Quote: The virus RNA in the cell or culture supernatant was extracted with nucleospin RNA plus (TaKaRa) and quantified by RT‒qPCR using Primer/Probe N2 (NIHON GENE RESEARCH LABORATORIES ...
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bioRxiv - Immunology 2022Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Immunology 2022Quote: ... Virus titer was determined by using Jurkat T cells and Lenti-X GoStix Plus (Takara Clontech).
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bioRxiv - Microbiology 2023Quote: ... RNA was purified from the medium with NucleoSpin® RNA Virus kit (U0956A, Takara Bio Inc.) by following the manufacture’s instruction and subjected to RT-qPCR with the primer pairs for EGFP (5′-CAAGCTGACCCTGAAGTTCATCTG-3′ and 5′-TTGAAGAAGTCGTGCTGCTTCATG-3′ ...
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bioRxiv - Plant Biology 2023Quote: ... under the control of cauliflower mosaic virus 35S promoter in the binary vector pBI121 (Clontech, USA) was transferred into Agrobacterium tumefaciens strain GV3101 and used to transform Arabidopsis by floral dip method and tobacco plants by leaf disk method (Horsch et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Adeno-associated virus (AAV) cell-lysates were produced using the AAVpro Purification Kit (All Serotypes) (Takara) with slight modifications ...
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bioRxiv - Plant Biology 2020Quote: ... All experiments were performed in Y2HGold yeast strain as per the manufacturer’s protocol (Clontech). The plates and liquid cultures were grown in incubator at 30°C with agitation (200 rpm ...
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bioRxiv - Plant Biology 2021Quote: pGBKT7-and pGADT7-based constructs were co-transformed into the Y2HGold yeast strain (Clontech) using Yeastmaker™ Yeast Transformation System 2 (Clontech ...
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bioRxiv - Plant Biology 2019Quote: ... Using the co-transformation techniques41 these constructs were transformed into the AH109 strain (Clontech).
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bioRxiv - Genetics 2019Quote: ... Plasmids were co-transformed into yeast strain EGY48 according to the manufacturer’s manual (Clontech). Transformed yeast was plated onto a synthetic medium DDO (-Ura/-Trp) ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids were co-transformed pairwise into AH109 yeast strains (Matchmaker 3 System, Clontech), and selected initially on double drop-out (DDO ...
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bioRxiv - Plant Biology 2023Quote: ... These bait and prey vectors were co-transfected into yeast strain Y2H GOLD (Clontech). The transfected yeast cells were screened on SD/-Leu/-Trp ...
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bioRxiv - Biochemistry 2023Quote: The plasmid library was transformed into Escherichia coli strain BL21(DE3)-pGro7/GroEL (TaKaRa). Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Cell Biology 2023Quote: ... Pairwise interactions were carried out in the Y2HGold Yeast strain (Cat. no. 630498, Clontech). For transformation of Y2HGold with bait and prey vectors ...
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bioRxiv - Cell Biology 2023Quote: ... Clones in pGADT7 and pGBKT7 were transformed into yeast strain AH109 (Takara Bio Inc.). The yeasts were grown on SD-Leu-Trp plates at 30°C ...
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bioRxiv - Genomics 2020Quote: ... The titer of each virus was determined using the Lenti-X Go-Stix Plus kit (Takara Bio), according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2019Quote: ... Whole genomic amplification of the influenza virus was conducted using Ex Taq™ Hot Start Version (TaKaRa). Forward primers were Uni-1.5 and Uni-0.5 mixed in a ratio of 3:2 ...
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bioRxiv - Synthetic Biology 2022Quote: Pantropic vesicular stomatitis virus G pseudotyped lentivirus was produced via transfection of LentiX 293T cells (Clontech #11131D) with a pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using FuGENE HD (Promega ...
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bioRxiv - Microbiology 2022Quote: ... LASVpp-BlaM virus was concentrated 10 times with Lenti-X concentrator (Clontech Laboratories, Mountain View, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 units of SMART Moloney murine leukemia virus reverse transcriptase (Takara Bio, Mountain View, CA, USA). The RT reaction was carried out at 42°C for 2 hrs ...
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bioRxiv - Cell Biology 2019Quote: Virus titer was determined by quantitative Polymerase Chain Reaction (qPCR) using Adeno-X qPCR Titration Kit (Clontech) on an Applied Biosystems 7900HT.
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bioRxiv - Immunology 2020Quote: ... and total RNA was reverse-transcribed to cDNA with Moloney Murine Leukemia Virus Reverse Transcriptase (2641B, TAKARA). Real-time quantitative PCR (qPCR ...