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Citations for Takara Bio :
201 - 250 of 2519 citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... modified for In-Fusion cloning (Clontech) using Kapa polymerase (Kapa biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... or In-Fusion cloning (Takara Bio) unless noted otherwise.
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bioRxiv - Cancer Biology 2022Quote: ... using In-Fusion HD (Takara Bio) following the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2022Quote: ... using In-Fusion HD (Takara Bio) following the manufacturer’s manual ...
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bioRxiv - Synthetic Biology 2020Quote: ... followed by In-Fusion (Takara Bio) or direct synthesis of shorter inserts followed by ligation into pL0 backbones ...
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bioRxiv - Bioengineering 2021Quote: ... using In-Fusion ligase (TaKaRa Bio). XLone-SOX7 and XLone-SOX18 were cloned into XLone by Genewiz ...
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bioRxiv - Neuroscience 2021Quote: ... using In-Fusion (Takara Bio #639649) or Gibson Assembly (NEB #E2611S ...
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bioRxiv - Neuroscience 2021Quote: ... or In-Fusion Cloning (Takara Bio). Details of primer sets ...
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bioRxiv - Microbiology 2021Quote: ... In Fusion HD Cloning (Takara #638920) was utilized to synthesize tat mutants on pHIV-1NL4-3-Δnef-eGFP background ...
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bioRxiv - Microbiology 2020Quote: ... and cloned using In-Fusion (Clontech) into the XhoI and SacII sites of pAMD179 to generate pAMD211 (targets codA+ protospacer ...
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bioRxiv - Microbiology 2020Quote: ... and cloning using In-Fusion (Clontech) into the HindIII site of pSDS009 to generate pSK014 (stp2 ...
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). pIZ-Siwi-D670A and pIZ-BmAgo3-D697A mutants were generated by site-directed mutagenesis.
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bioRxiv - Biochemistry 2022Quote: ... by In-fusion cloning kit (Takara). For Gtsf1L ...
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bioRxiv - Cell Biology 2022Quote: ... or by In-Fusion cloning (Takara). FRB-BFP control and FRB-Ubiquitin-BFP constructs were generated by fragment synthesis and cloned into the retroviral expression vector pQCXIH ...
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bioRxiv - Genetics 2022Quote: FIREWACh RE In-Fusion cloning (Takara) was carried out in a 2:1 insert/vector ratio ...
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bioRxiv - Neuroscience 2023Quote: ... In-Fusion HD Cloning Kit (Clontech), and primers which included NNK codons ...
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bioRxiv - Plant Biology 2023Quote: ... The In-Fusion system (TaKaRa Bio) was employed to assemble the amplified sequences and green fluorescent protein (GFP ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion Cloning kit (Takara).
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bioRxiv - Neuroscience 2023Quote: ... by In-Fusion recombinase (Clontech Laboratories). The ExRai-AKAR2 cDNA codon was optimized for Drosophila melanogaster (Eurofins Genomics) ...
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bioRxiv - Cell Biology 2023Quote: ... In-Fusion® Snap Assembly (Takara).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and In-Fusion Snap Assembly (Takara) site-directed mutagenesis as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: In Fusion HD cloning (Takara 638910) was used to switch full length WT mouse Myo10 in EGFP-C1 to mScarlet-C1 ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion cloning (Takara Bio) with primer pairs ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... or In-Fusion (Clontech Cat# 638947). A detailed list of the plasmids is provided in Supplementary Table S1 ...
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bioRxiv - Biophysics 2023Quote: ... by In-Fusion ligation (Takara Bio) and transformed into Rosetta 2 E ...
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bioRxiv - Molecular Biology 2023Quote: ... by In-fusion cloning kit (Takara).
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bioRxiv - Molecular Biology 2023Quote: ... by In-fusion cloning kit (Takara).
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion cloning kit (Takara). We verified the sequences of both constructs pHR-[SFFV/FLAG::TurboID] and pHR-[SFFV/FLAG::TurboID::SQSTM1] using Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... using In-Fusion HD (Takara Bio). ACs C-terminally tagged with muGFP44 were generated by removing EGFP from pEGFP-N1 vector by digestion with AgeI and NotI followed by PCR amplification of muGFP (a gift from Mullins lab ...
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bioRxiv - Evolutionary Biology 2023Quote: ... by In-Fusion cloning (Takara Bio). Verified plasmids were then introduced into Agrobacterium strain AGL1 by a freeze-thaw method (85) ...
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bioRxiv - Molecular Biology 2023Quote: ... In-Fusion HD Cloning Kit (Clontech) was used for this reaction.
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bioRxiv - Cell Biology 2023Quote: ... or In-Fusion cloning (Takara Bio). Full-length sequences of plasmids used in this study will become available at a public data share server upon publication.
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bioRxiv - Cell Biology 2019Quote: ... The expressed peptide was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... The expressed peptide was purified using hexa-histidine-tag and GST-tag by Talon-resin (Clontech/Takara) or Glutathione-sepharose (GE healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... downstream of the Myh6 (α- myosin heavy chain) promoter and upstream of a human growth hormone polyadenylation signal using In-Fusion HD (Takara Bio cat# 011614). The final transgenic targeting construct was verified by DNA sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were transfected with a pMXs-puro vector carrying hXkr8-GFP or a pCX4-bsr vector carrying hBSG cDNA tagged with HA or FLAG together with pGP for the gag-pol fusion protein (Takara Bio), and pCMV-VSV-G-RSV-Rev (RIKEN) ...
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bioRxiv - Plant Biology 2020Quote: ... All of the other genes encoding candidate RBOHD-associated proteins were cloned into epiGreenB5 by the same strategy using an In-Fusion HD Cloning Kit (Clontech, USA). To generate epiGreenB-pPB1CP::PB1CP-3-eGFP for transient expression in N ...
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bioRxiv - Immunology 2021Quote: ... and the NES sequence of the HIV-1 rev protein into pT2ADW vector (Komatsu et al., 2018) by In-Fusion cloning (Takara Bio).
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bioRxiv - Plant Biology 2023Quote: The gene encoding the PP1c1-293 protein (herein simply referred to as PP1c) was cloned into the pOPIN-SC3 vector (OPPF) via In-Fusion Cloning (Takara Bio), which provides a cleavable N-terminal 6xHIS-SUMO tag for purification and solubility ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... pCSIIhyg-R-GECO1 or pCSIIbsr-GCaMP6s was cotransfected with psPAX2 and pCMV-VSV-G-RSV-Rev into Lenti-X 293T cells (Clontech, Mountain View, CA) with Polyethylenimine “Max” (Mw 40,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... In-Fusion cloning was performed using the In-Fusion® HD Cloning Plus Kit (Takara Bio, Japan) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... 1.85U recombinant RNase Inhibitor (Takara), 1.85 μM template-switching oligo was aliquoted to each lysed cell using a Nanodrop II liquid handling system (BioNex ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilised metal affinity chromatography (IMAC using Talon resin; Clontech) as described previously (34) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The recombinant proteins were purified using a TALON Metal (Cobalt) Affinity Resin column (Clontech Laboratories, Inc., Palo Alto, CA, USA) and eluted with a linear gradient of imidazole (0–1,000 mM ...
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bioRxiv - Biochemistry 2019Quote: ... anti-GFP-tag (Living Colors 632592; Clontech), anti-Strep-tag (34850 ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were induced by 1 mM IPTG at 16°C for 20 h, then purified by a GST-tag Protein Purification Kit (Beyotime, Shanghai, China) or His TALON Purification Kit (Takara, Beijing, China). Corresponding primers are listed in Supplemental Table S2.
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bioRxiv - Developmental Biology 2024Quote: ... the C-terminal twin strep tag was removed by incubating with the 3C protease (Takara Bio, Japan; 1.5 unit/50 mg protein) and ran on a size-exclusion column ...
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bioRxiv - Microbiology 2020Quote: ... The virus was concentrated using Lenti-X Concentrator (Clontech). Lentivirus titers from 48h and 72h were determined by plaque assay (data not shown ...
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bioRxiv - Immunology 2019Quote: ... and virus was concentrated using Lenti-X Concentrator (Takara). Existing mIRAK1mCherry and Irak1−/− iBMDM were then transduced with concentrated lentivirus ...