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Citations for Takara Bio :
351 - 400 of 1520 citations for Recombinant Human FCGRT & B2M Protein His Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... test genes (vascular connexins) in human vessels were normalized relative to human whole heart (RNA purchased from Clontech) and in mouse ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA of Human Phosphodiesterase type 10 (hPDE10) was amplified by conventional nested PCR using human brain cDNA (Clontech). cDNAs template encoding wild-type and D674A mutant form of hPDE10 catalytic domain (CD ...
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bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR templates were generated by PCR reaction with biotinylated primers using high fidelity ClonAmp HiFi PCR mix (Takara Inc.). A mixture of Cas9-mSA mRNA (75ng/μl) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR templates were generated by PCR reaction with biotinylated primers using high fidelity ClonAmp HiFi PCR mix (Takara Inc.). A mixture of Cas9-mSA mRNA (75ng/ml) ...
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bioRxiv - Genetics 2020Quote: ... Full length wild type NHR-25 was tagged with EGFP at its N-terminus using pEGFP-C2 plasmid vector (Clontech); wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: The cDNAs for HA-tagged ELF3 were amplified from the KhES-1 cDNA library by PCR using PrimeSTAR GXL (Takara) and were subcloned into the pENTR/D entry vector (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: Expression plasmid for mouse FLAG tagged BAG3 (pFLAG-BAG3) was constructed by cloning partial mouse BAG3 cDNA containing the whole CDS into pEGFP-N1 (Clontech). Forward primer sequence used to clone BAG3 plasmid is 5′-AAAGGATCCAGCGCCGCCACCC-3′ and the reverse primer sequence is 5′-GACTCTAGATCACTAGGGAGCCACCAGGTTGC-3′ ...
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bioRxiv - Cell Biology 2021Quote: The FKBP sequence was tagged to a 3xFLAG-LRRK2 vector using IN-FUSION HD cloning technology (Clontech, Takara, cat #638920). LYSO-LRRK2 was created by adding the N terminal domain of the LAMTOR1 sequence (aa 1-39 ...
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bioRxiv - Cell Biology 2021Quote: The FKBP sequence was tagged to a 3xFLAG-LRRK2 vector using IN-FUSION HD cloning technology (Clontech, Takara, cat #638920). LYSO-LRRK2 was created by adding the N terminal domain of the LAMTOR1 sequence (aa 1-39 ...
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bioRxiv - Cancer Biology 2019Quote: ... This plasmid was used as a template to generate GFP-tagged mutant versions Cdc42ep5GPS-AAA using In-Fusion cloning (Takara). To allow for lentiviral infection and stable expression ...
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bioRxiv - Cell Biology 2021Quote: ... WT TARPs and ncAA-tagged TARPs were subcloned into a bidirectional doxycycline-inducible expression vector pTRE3G-BI (Takara Bio, #631332) using the restriction sites KpnI/XbaI ...
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bioRxiv - Microbiology 2019Quote: ... TetON-IRE1-GFP MEFs were obtained by reconstituting Ire1-/- MEFs with GFP-tagged murine IRE1 under control of a ‘tight’ Tet-responsive element (Clontech) essentially as described (Bakunts et al ...
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bioRxiv - Cell Biology 2021Quote: ... pDAA-026 was generated by subcloning a DNA fragment encoding an N-terminus FLAG-tagged mouse eIF2α coding sequence flanked by BamHI and EcoRI sites into the BglII and EcoRI sites of pLPCX (Clontech) using standard molecular biology methods ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Neuroscience 2022Quote: ... with RNAse-free water with 0.4 U/μL Recombinant RNase Inhibitor (Takara Clontech, Ref. 2313A) and a fresh dilution of 1 in 300,000 was prepared before the first strand synthesis.
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bioRxiv - Microbiology 2024Quote: ... The recombinant construct was transformed into Escherichia coli StellarTM Competent Cells (dam–/dcm–) (Takara Bio) according to the supplier’s protocol (ClonTech ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in PBS with 0.3%BSA and 1% recombinant RNase inhibitor (RRI, Takara Bio). Next ...
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bioRxiv - Biophysics 2021Quote: ... SYBR Premix Ex Taq II (Takara, Otsu, Shiga, Japan) on StepOnePlus Real Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... using the SYBR premix ExTaq™ II (Takara, Japan) in 96-well optical reaction Roche plates ...
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bioRxiv - Molecular Biology 2019Quote: ... with SYBR Premix Ex Taq II (TaKaRa, Dailian, China). The reaction was carried out at a final volume of 15.0 μL ...
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bioRxiv - Plant Biology 2021Quote: ... USA) using SYBR Premix Ex Tap II kit (Takara). Data represent the calculation based on the mean threshold cycle (Ct ...
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bioRxiv - Plant Biology 2020Quote: ... using the SYBR Premix Ex Taq II kit (TAKARA). The primers used for qPCR were reported previously45 ...
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bioRxiv - Neuroscience 2020Quote: ... with SYBR Premix Ex Taq TM II (Takara Bio). The specificity of amplicons was examined by melting curve analysis and agarose gel electrophoresis ...
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bioRxiv - Plant Biology 2021Quote: ... and TB Green Premix Ex Taq II (Takara, Japan). The following PCR conditions were used ...
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bioRxiv - Immunology 2022Quote: ... using SYBR Premix Ex Taq II mix (TaKaRa Bio) as described [8] ...
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bioRxiv - Plant Biology 2022Quote: ... 10 μL of SYBR Premix Ex TaqTM II (Takara), and 0.4μL of reference dye ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... and TB Green Premix EX Taq II (TAKARA Bio) (Fujimoto et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... and PrimeScript II 1st strand cDNA Synthesis Kit (Takara). The coding sequences of the corresponding genes were amplified by PrimeSTAR Max DNA Polymerase (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... and TB Green Premix Ex Taq II (Takara Bio). Gene expression levels were measured using TaqMan® gene expression assay kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... using the SYBR Premix Ex Taq II (Takara, Japan), using a BioRad Chromo4 real-time PCR machine ...
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bioRxiv - Immunology 2020Quote: ... with TB Green Premix Ex Taq II (RR820A; Takara). The primer sequences used in this study are listed in Table 2.
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bioRxiv - Cell Biology 2021Quote: ... or TB Green Premix Ex Taq II (Takara, RR820A) was used according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... using TB Green Premix Ex Taq II (Takara, Japan). The qRT-PCR assays were performed with technical triplicates of three biological replicates ...
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bioRxiv - Immunology 2022Quote: ... or TB Green Premix Ex Taq II (TaKaRa Bio). All data are presented as relative expression levels normalized to Hprt expression ...
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bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). All primers used in this study are listed in Table S5 (No ...
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bioRxiv - Microbiology 2022Quote: ... and Thermal Cycler Dice Real Time System II (Takara). Three biologically independent experiments were performed and used for the analysis.
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bioRxiv - Plant Biology 2022Quote: ... 10 μl SYBR Premix Ex Taq II (Takara, Japan), and nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... on Thermal Cycler Dice Real Time System II (TaKaRa). The relative mitochondrial DNA content normalized by nuclear-genome content was determined using the ΔΔCt method.
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bioRxiv - Genetics 2023Quote: ... and TB Green Premix Ex TaqTM II (Takara, RR820A) in a StepOne real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... and SYBR Premix Ex Taq II (Takara, Shiga, Japan) with gene-specific primers (S4 Table ...
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bioRxiv - Cell Biology 2023Quote: ... and TB Green Premix Ex Taq II (TaKaRa Bio). In each sample ...
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bioRxiv - Cell Biology 2023Quote: ... using SYBR® Premix Ex Taq™ II (TaKaRa). Specific primers for RPL13 ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µL SYBR Premix Ex Taq II (Takara, China), 1 µL (200 nM final concentration ...
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bioRxiv - Cell Biology 2024Quote: ... using SYBR® Premix Ex TaqTM II (TaKaRa, Japan). All primer information for each target gene was obtained from the Primerbank website(https://pga.mgh.harvard.edu/primerbank/) ...
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bioRxiv - Plant Biology 2022Quote: ... and His (-HTLA) but containing 5-bromo-4-chloro-3-indolyl α-D-galactopyranoside (Clontech, Madison, WI, USA). As a control ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
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bioRxiv - Cell Biology 2023Quote: ... RNA-seq libraries were prepared with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara #634875). 75 bp single-end sequencing was performed using an Illumina NextSeq500 system at the CRI at UT Southwestern Sequencing Facility ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio USA, Inc.), which incorporates both RiboGone and SMART (Switching Mechanism At 5’ end of RNA Template ...